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The map label for this gene is oprN [H]

Identifier: 21241209

GI number: 21241209

Start: 518627

End: 520138

Strand: Reverse

Name: oprN [H]

Synonym: XAC0436

Alternate gene names: 21241209

Gene position: 520138-518627 (Counterclockwise)

Preceding gene: 21241215

Following gene: 21241206

Centisome position: 10.05

GC content: 69.91

Gene sequence:

>1512_bases
ATGTCGTCACTCCGCACCCTCGCAGCGCTCGTTGTCGCCAGCCTCGTCACAGGTTGCGCGGTTGGTCCCGACTACGCCCG
GCCGGAGGCGCCACTGCCGGACCGCTACATCGGCCAGGCGGATGTGGCACAGCGACAGGCCGCAGTGCCAACCGAGCTGT
CGACCTGGAGGGACGGCTTCAACGATCCCGACCTAAGCCGCTACGTCAACGCGGCGCTGGCACAAAACCTGGACCTGGCC
CAAGCCGGCGCGCGCGTGGACCCGGCGCGCGCCGGGCTGAGTGCCGCCACCGCCGCGCTGCTACCGGCGGGCAATGTGAG
CGGCAGCGCCACGCGTGCCTACCAATCGCTGGAAACACCGTTGGGCCAGGTGCTCAACAGCACGCCCAACTTCGACCGCC
ATGCCAGTGTCTACGAAGCCAACCTGGGCGCCAGCTGGGAGCTCGATCTGTTCGGGGGCCTGCGCCGCGGCCGCCAGGCG
GCCTTGGCCGACTACCAGGCCACCGAAGCAGGCGTCGCCGCCACCCGCCTGGCAGTCGCCGCGCAGACCGCCGATATCTA
CATCGGCGTCCGCGGCTTGCAGGCGCGGCTGGATGTGGCGCAGCGCCAGGTGGCCACCCAGCAGGGCCTGCTCTCGATGG
TGACGCTCCTGCACGGCAAGGGCCTGGCCGCCGAGCTGCAACTGCAGCAGGCCCAGGGCGCGCTCGCGCAGGCGCAAGCC
GGCGTGCCAGGTCTGGAAGCAGGCCTGGTTGCGGCAATGAACGCGCTGGACGCCATGCTTGGCGTGCCGCCGGGCACCCA
CCGCGCCGAGCTGTCGGCGGCGATCAAACCCATTCCGCCGGCCCCGCAGATCGCCGACAGCGGCGCCCCCGGCGACCTGC
TACGGCGCCGGCCCGACCTGATCGTGGCCGAGCGCCGGCTGGCCGCCTCCAACGCGCGCATCGGCGTGGCGATGGCCGAG
TACTACCCCAAATTTTCGCTCAGCGGCCTGCTTGGCAGCGCCACGGCCATCTCCGGTGCGAACCTGTTTACCGGCGACGC
CGCGCAATCGTCCGGCGTGCTCGGCCTGCGCTGGCGGCTGTTCGACTTCGGCCGCATCAATGCACAGATCGCACAGGCCA
AAGGCCAGCAAGCCGAACAACTGGCGGCTTATCGCCTGGCGGTGTTGCGCGCCACCGAAGACGTCGAAAATGCGTTCACC
GCACTGATCAAACGCGAGCAGCAGGCAGTGGTGCTGACGCAAGGCGTGGACGCACTCGGCAAGGCGCGCGGCGCTTCTGC
GCTGGCCTACGAAAAAGGCACGGTGAGCCTGATCGAAGTGCTCAACGCCGACGACAGCCTGCTAAGCGCCTCCGACGCGC
TGGTGCAGGCGCGTACCGACGCGGCGCACAGCGCGGTGGCGACCTTCAAGGCGCTGGGCGGCGGTTGGCAGGCCGGCGGA
GCGGATGCGGGTGCAATCGCTGTCGCAGTGGACAGCAATCGTCGACACGACGACACGACGGCAACCGAGTGA

Upstream 100 bases:

>100_bases
GATGCTTTGCTCATCGCGACAGCTTAGCGCCGTCGCTACCGACTTTCAAAAGTGACTACTTCGTATTATAGTCACTGAAC
TTTCTCGGTCCGGCCCCGCC

Downstream 100 bases:

>100_bases
ATGACGCGGCACGCTGCCGTTGAGGGGTAACTGCGCACGTGGCACCCCACTGCTGCCGATGCCGCCGCGCGCATGCAACC
GCAAGAGCTGACCAACCGCG

Product: outer membrane protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 503; Mature: 502

Protein sequence:

>503_residues
MSSLRTLAALVVASLVTGCAVGPDYARPEAPLPDRYIGQADVAQRQAAVPTELSTWRDGFNDPDLSRYVNAALAQNLDLA
QAGARVDPARAGLSAATAALLPAGNVSGSATRAYQSLETPLGQVLNSTPNFDRHASVYEANLGASWELDLFGGLRRGRQA
ALADYQATEAGVAATRLAVAAQTADIYIGVRGLQARLDVAQRQVATQQGLLSMVTLLHGKGLAAELQLQQAQGALAQAQA
GVPGLEAGLVAAMNALDAMLGVPPGTHRAELSAAIKPIPPAPQIADSGAPGDLLRRRPDLIVAERRLAASNARIGVAMAE
YYPKFSLSGLLGSATAISGANLFTGDAAQSSGVLGLRWRLFDFGRINAQIAQAKGQQAEQLAAYRLAVLRATEDVENAFT
ALIKREQQAVVLTQGVDALGKARGASALAYEKGTVSLIEVLNADDSLLSASDALVQARTDAAHSAVATFKALGGGWQAGG
ADAGAIAVAVDSNRRHDDTTATE

Sequences:

>Translated_503_residues
MSSLRTLAALVVASLVTGCAVGPDYARPEAPLPDRYIGQADVAQRQAAVPTELSTWRDGFNDPDLSRYVNAALAQNLDLA
QAGARVDPARAGLSAATAALLPAGNVSGSATRAYQSLETPLGQVLNSTPNFDRHASVYEANLGASWELDLFGGLRRGRQA
ALADYQATEAGVAATRLAVAAQTADIYIGVRGLQARLDVAQRQVATQQGLLSMVTLLHGKGLAAELQLQQAQGALAQAQA
GVPGLEAGLVAAMNALDAMLGVPPGTHRAELSAAIKPIPPAPQIADSGAPGDLLRRRPDLIVAERRLAASNARIGVAMAE
YYPKFSLSGLLGSATAISGANLFTGDAAQSSGVLGLRWRLFDFGRINAQIAQAKGQQAEQLAAYRLAVLRATEDVENAFT
ALIKREQQAVVLTQGVDALGKARGASALAYEKGTVSLIEVLNADDSLLSASDALVQARTDAAHSAVATFKALGGGWQAGG
ADAGAIAVAVDSNRRHDDTTATE
>Mature_502_residues
SSLRTLAALVVASLVTGCAVGPDYARPEAPLPDRYIGQADVAQRQAAVPTELSTWRDGFNDPDLSRYVNAALAQNLDLAQ
AGARVDPARAGLSAATAALLPAGNVSGSATRAYQSLETPLGQVLNSTPNFDRHASVYEANLGASWELDLFGGLRRGRQAA
LADYQATEAGVAATRLAVAAQTADIYIGVRGLQARLDVAQRQVATQQGLLSMVTLLHGKGLAAELQLQQAQGALAQAQAG
VPGLEAGLVAAMNALDAMLGVPPGTHRAELSAAIKPIPPAPQIADSGAPGDLLRRRPDLIVAERRLAASNARIGVAMAEY
YPKFSLSGLLGSATAISGANLFTGDAAQSSGVLGLRWRLFDFGRINAQIAQAKGQQAEQLAAYRLAVLRATEDVENAFTA
LIKREQQAVVLTQGVDALGKARGASALAYEKGTVSLIEVLNADDSLLSASDALVQARTDAAHSAVATFKALGGGWQAGGA
DAGAIAVAVDSNRRHDDTTATE

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=474, Percent_Identity=26.7932489451477, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI2367348, Length=496, Percent_Identity=25, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 51949; Mature: 51818

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLRTLAALVVASLVTGCAVGPDYARPEAPLPDRYIGQADVAQRQAAVPTELSTWRDGF
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHCCC
NDPDLSRYVNAALAQNLDLAQAGARVDPARAGLSAATAALLPAGNVSGSATRAYQSLETP
CCCHHHHHHHHHHHCCCCHHHCCCCCCHHHHCHHHHHHHHCCCCCCCCHHHHHHHHHHHH
LGQVLNSTPNFDRHASVYEANLGASWELDLFGGLRRGRQAALADYQATEAGVAATRLAVA
HHHHHCCCCCCCCCCHHEECCCCCCEEEHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
AQTADIYIGVRGLQARLDVAQRQVATQQGLLSMVTLLHGKGLAAELQLQQAQGALAQAQA
EEECEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHC
GVPGLEAGLVAAMNALDAMLGVPPGTHRAELSAAIKPIPPAPQIADSGAPGDLLRRRPDL
CCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCC
IVAERRLAASNARIGVAMAEYYPKFSLSGLLGSATAISGANLFTGDAAQSSGVLGLRWRL
EEHHHHHHHCCCCEEEEHHHHCCCHHHHHHHCCHHHCCCCCEEECCCCCCCCEEEEEEEE
FDFGRINAQIAQAKGQQAEQLAAYRLAVLRATEDVENAFTALIKREQQAVVLTQGVDALG
EECCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHH
KARGASALAYEKGTVSLIEVLNADDSLLSASDALVQARTDAAHSAVATFKALGGGWQAGG
HHCCCCEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
ADAGAIAVAVDSNRRHDDTTATE
CCCCEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
SSLRTLAALVVASLVTGCAVGPDYARPEAPLPDRYIGQADVAQRQAAVPTELSTWRDGF
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHCCC
NDPDLSRYVNAALAQNLDLAQAGARVDPARAGLSAATAALLPAGNVSGSATRAYQSLETP
CCCHHHHHHHHHHHCCCCHHHCCCCCCHHHHCHHHHHHHHCCCCCCCCHHHHHHHHHHHH
LGQVLNSTPNFDRHASVYEANLGASWELDLFGGLRRGRQAALADYQATEAGVAATRLAVA
HHHHHCCCCCCCCCCHHEECCCCCCEEEHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
AQTADIYIGVRGLQARLDVAQRQVATQQGLLSMVTLLHGKGLAAELQLQQAQGALAQAQA
EEECEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHC
GVPGLEAGLVAAMNALDAMLGVPPGTHRAELSAAIKPIPPAPQIADSGAPGDLLRRRPDL
CCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCC
IVAERRLAASNARIGVAMAEYYPKFSLSGLLGSATAISGANLFTGDAAQSSGVLGLRWRL
EEHHHHHHHCCCCEEEEHHHHCCCHHHHHHHCCHHHCCCCCEEECCCCCCCCEEEEEEEE
FDFGRINAQIAQAKGQQAEQLAAYRLAVLRATEDVENAFTALIKREQQAVVLTQGVDALG
EECCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHH
KARGASALAYEKGTVSLIEVLNADDSLLSASDALVQARTDAAHSAVATFKALGGGWQAGG
HHCCCCEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
ADAGAIAVAVDSNRRHDDTTATE
CCCCEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]