Click here to switch to the map view.

The map label for this gene is smeC [H]

Identifier: 21241103

GI number: 21241103

Start: 392943

End: 394409

Strand: Direct

Name: smeC [H]

Synonym: XAC0329

Alternate gene names: 21241103

Gene position: 392943-394409 (Clockwise)

Preceding gene: 21241102

Following gene: 21241108

Centisome position: 7.59

GC content: 65.03

Gene sequence:

>1467_bases
GTGATGCGCTACGGCTTTGCAAGTGTCGCAGTGGCCTGCGCCATCGCCTTGAGTGGCTGCGTCAGCATGGCGCCGCATTA
CGCACGCCCGGATGCGCCGGTGCCCAGCGTGATCGGCAGCACCGGCAGCACCGGCAACGCGGATACCGGCACGCCGAATG
CAGCCGCTCTGGATTGGCGGCAGGTGGTGACCGATGCGCGTCTGCAGCAGGTGGTCGCGCTGGCGCTGGACAACAACCGC
GACCTGCGCGTAGCAGTACTCAATATCGAGCAGGCACGCGCGCAGTACCGCATTCAACGTGCGAACCTGTTTCCCTCCGT
CGATGCCACCGTCTCGCACACCGCGCAGCGCGCCGCGGCAGCGACCAGTTTCACCGGCGCGGCGCAGGTCATCCGCAGCG
CCAGCGGCGAGGTGGGCATCAGCAGCTGGGAGCTGGATCTGTTCGGTCGCATCCGCAGCCTCAAGAACGAGGCCCTGGAA
AGCTATCTCGCCAGTGAGCAAACCCAGCGCAGCACGCGTCTGAGCCTGGTGGCTGAAGTGGCCAACGACTGGTTGACCGT
GGCTGCCGATCAACAGCGCCTGGCGCTCGCGCAGCAGACCCTGGACAGCCAACGCAAGACGCTGCGTCTGACCGAGCTGC
AGCACGACAACGGCATCGTCTCCGGTCTGGATCTGGCGCAGATCCAGACCAGCGTGGAAAGCGCGCGCGCTGATGTGGCC
AACTACGCCACCCAGCTGGCGCAGTCGCGCAACGCCTTGAATCTGGTGGTGGGCACGTCGGTGCCGGTTGCGTTGTTGCC
TGCCAGCGATGCGATCGAGACAGGCGTGGCGCTTGCGCCGTTGCCTGCGCAGCTGGAATCGCGGGTGTTGTTGCAGCGCC
CGGACGTGCTGCCGGCCGAACACACGCTCAAGGCCGCCAATGCAGATATCGGCGCGGCACGTGCGGCCTTCTTCCCCACC
ATCTCGTTGACCGCCAATGCCGGGCGCGGCAGCGATGCGTTATCCAGCCTGTTCGATGGCGACCATACGTGGTCGTTCTC
GCCCAGCGTGAGCGTGCCGATCTTCCGTGCCGGCGCGTTGAAGGCCGAACTGGATGTGGCCACGTTGCAAAAGGACATCA
CCATTGCGCAGTACGAATTGGCGATCCAGACCGCCTTCAGTGAAGTGGCCGATGCACTCGCCGAACGCACGCAGCTTGAA
GAACGGATGCATGCGCAACAGGCAATGGTGGACGCCGCACAACGCAGCTTCAGCCTATCGGATGCGCGCTATCGCAATGG
CGTGGACAGTTACCTGGTGGCGCTGGATGCGCAGCGCACGCTCTACAGCGCACAACAGACACTGATCAGCTTGCGGCTGA
CCGAGGCGAACAATCGGGTGACCTTGTACAAGGTGCTGGGTGGTGGCGCCGATGCACAATCGGCCGTGGCATCAACCCAA
GGCCCGGCGCCAGCCCTGCAGCGCTAG

Upstream 100 bases:

>100_bases
GCATCTTCTTCGTGCCGTTGTTCTACGTGATGGTGCGCAGCGTGTTTCCTGGTAGGGCGTCTGATCACGCGACAGCCGCT
GAGACGTCCGAGGTGGCCCC

Downstream 100 bases:

>100_bases
CGCCGGTTGCAACGCGATGGCCGGCGCACGCCCCGGCCATCGCACCGCAGGTCAACCCGCCTTACCTCGCCGCATTCGCA
TTCATCACTGCATTGCGGTT

Product: outer membrane protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MMRYGFASVAVACAIALSGCVSMAPHYARPDAPVPSVIGSTGSTGNADTGTPNAAALDWRQVVTDARLQQVVALALDNNR
DLRVAVLNIEQARAQYRIQRANLFPSVDATVSHTAQRAAAATSFTGAAQVIRSASGEVGISSWELDLFGRIRSLKNEALE
SYLASEQTQRSTRLSLVAEVANDWLTVAADQQRLALAQQTLDSQRKTLRLTELQHDNGIVSGLDLAQIQTSVESARADVA
NYATQLAQSRNALNLVVGTSVPVALLPASDAIETGVALAPLPAQLESRVLLQRPDVLPAEHTLKAANADIGAARAAFFPT
ISLTANAGRGSDALSSLFDGDHTWSFSPSVSVPIFRAGALKAELDVATLQKDITIAQYELAIQTAFSEVADALAERTQLE
ERMHAQQAMVDAAQRSFSLSDARYRNGVDSYLVALDAQRTLYSAQQTLISLRLTEANNRVTLYKVLGGGADAQSAVASTQ
GPAPALQR

Sequences:

>Translated_488_residues
MMRYGFASVAVACAIALSGCVSMAPHYARPDAPVPSVIGSTGSTGNADTGTPNAAALDWRQVVTDARLQQVVALALDNNR
DLRVAVLNIEQARAQYRIQRANLFPSVDATVSHTAQRAAAATSFTGAAQVIRSASGEVGISSWELDLFGRIRSLKNEALE
SYLASEQTQRSTRLSLVAEVANDWLTVAADQQRLALAQQTLDSQRKTLRLTELQHDNGIVSGLDLAQIQTSVESARADVA
NYATQLAQSRNALNLVVGTSVPVALLPASDAIETGVALAPLPAQLESRVLLQRPDVLPAEHTLKAANADIGAARAAFFPT
ISLTANAGRGSDALSSLFDGDHTWSFSPSVSVPIFRAGALKAELDVATLQKDITIAQYELAIQTAFSEVADALAERTQLE
ERMHAQQAMVDAAQRSFSLSDARYRNGVDSYLVALDAQRTLYSAQQTLISLRLTEANNRVTLYKVLGGGADAQSAVASTQ
GPAPALQR
>Mature_488_residues
MMRYGFASVAVACAIALSGCVSMAPHYARPDAPVPSVIGSTGSTGNADTGTPNAAALDWRQVVTDARLQQVVALALDNNR
DLRVAVLNIEQARAQYRIQRANLFPSVDATVSHTAQRAAAATSFTGAAQVIRSASGEVGISSWELDLFGRIRSLKNEALE
SYLASEQTQRSTRLSLVAEVANDWLTVAADQQRLALAQQTLDSQRKTLRLTELQHDNGIVSGLDLAQIQTSVESARADVA
NYATQLAQSRNALNLVVGTSVPVALLPASDAIETGVALAPLPAQLESRVLLQRPDVLPAEHTLKAANADIGAARAAFFPT
ISLTANAGRGSDALSSLFDGDHTWSFSPSVSVPIFRAGALKAELDVATLQKDITIAQYELAIQTAFSEVADALAERTQLE
ERMHAQQAMVDAAQRSFSLSDARYRNGVDSYLVALDAQRTLYSAQQTLISLRLTEANNRVTLYKVLGGGADAQSAVASTQ
GPAPALQR

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=463, Percent_Identity=38.6609071274298, Blast_Score=303, Evalue=2e-83,
Organism=Escherichia coli, GI2367348, Length=485, Percent_Identity=24.7422680412371, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 51835; Mature: 51835

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRYGFASVAVACAIALSGCVSMAPHYARPDAPVPSVIGSTGSTGNADTGTPNAAALDWR
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHH
QVVTDARLQQVVALALDNNRDLRVAVLNIEQARAQYRIQRANLFPSVDATVSHTAQRAAA
HHHHHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
ATSFTGAAQVIRSASGEVGISSWELDLFGRIRSLKNEALESYLASEQTQRSTRLSLVAEV
HHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ANDWLTVAADQQRLALAQQTLDSQRKTLRLTELQHDNGIVSGLDLAQIQTSVESARADVA
HHCCEEEECCHHHHHHHHHHHHHHHHHEEHHHEECCCCCCCCCHHHHHHHHHHHHHHHHH
NYATQLAQSRNALNLVVGTSVPVALLPASDAIETGVALAPLPAQLESRVLLQRPDVLPAE
HHHHHHHHCCCCEEEEEECCCCEEEECCCHHHHCCCEECCCCHHHHHHHHHCCCCCCCHH
HTLKAANADIGAARAAFFPTISLTANAGRGSDALSSLFDGDHTWSFSPSVSVPIFRAGAL
HHHHHCCCCCCHHHHHHCCEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCEEECCCC
KAELDVATLQKDITIAQYELAIQTAFSEVADALAERTQLEERMHAQQAMVDAAQRSFSLS
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DARYRNGVDSYLVALDAQRTLYSAQQTLISLRLTEANNRVTLYKVLGGGADAQSAVASTQ
HHHHHCCHHHHHEEHHHHHHHHHHHHHHHEEEEECCCCEEEEEEEECCCCCHHHHHHCCC
GPAPALQR
CCCCCCCC
>Mature Secondary Structure
MMRYGFASVAVACAIALSGCVSMAPHYARPDAPVPSVIGSTGSTGNADTGTPNAAALDWR
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHH
QVVTDARLQQVVALALDNNRDLRVAVLNIEQARAQYRIQRANLFPSVDATVSHTAQRAAA
HHHHHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
ATSFTGAAQVIRSASGEVGISSWELDLFGRIRSLKNEALESYLASEQTQRSTRLSLVAEV
HHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ANDWLTVAADQQRLALAQQTLDSQRKTLRLTELQHDNGIVSGLDLAQIQTSVESARADVA
HHCCEEEECCHHHHHHHHHHHHHHHHHEEHHHEECCCCCCCCCHHHHHHHHHHHHHHHHH
NYATQLAQSRNALNLVVGTSVPVALLPASDAIETGVALAPLPAQLESRVLLQRPDVLPAE
HHHHHHHHCCCCEEEEEECCCCEEEECCCHHHHCCCEECCCCHHHHHHHHHCCCCCCCHH
HTLKAANADIGAARAAFFPTISLTANAGRGSDALSSLFDGDHTWSFSPSVSVPIFRAGAL
HHHHHCCCCCCHHHHHHCCEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCEEECCCC
KAELDVATLQKDITIAQYELAIQTAFSEVADALAERTQLEERMHAQQAMVDAAQRSFSLS
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DARYRNGVDSYLVALDAQRTLYSAQQTLISLRLTEANNRVTLYKVLGGGADAQSAVASTQ
HHHHHCCHHHHHEEHHHHHHHHHHHHHHHEEEEECCCCEEEEEEEECCCCCHHHHHHCCC
GPAPALQR
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]