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The map label for this gene is rluE [H]

Identifier: 21241064

GI number: 21241064

Start: 344222

End: 344800

Strand: Direct

Name: rluE [H]

Synonym: XAC0290

Alternate gene names: 21241064

Gene position: 344222-344800 (Clockwise)

Preceding gene: 21241063

Following gene: 21241076

Centisome position: 6.65

GC content: 67.53

Gene sequence:

>579_bases
TTGGCAATGTTGGTGTTGTTGAACAAACCTTACGGTGTGCTCTCGCAATTCAGCGACCGCTCGCAGCCGCCCAAGCGCAC
GCTGGCCGAATTCGGCTTGCCGCCCGATGTCTATGCCGCAGGCCGTCTGGACCATGACAGCGAAGGCTTGCTGGTGCTGA
CCGACGACGGTGCATTGGCGCATCGGCTGACCGACCCGCGCCATAAACAGCCCAAGACGTACTGGGTACAAGTCGAAGGC
GCGCCACAAGCCGAACACCTGCAGGCATTGCGCGACGGGGTGGCGCTCAACGATGGCCCCACCCGGCCGGCACAGGTGCG
CATGCTCGACCCGGCACCGCAGCTGTGGCCGCGCGATCCACCGGTGCGCGTGCGCAAGACGGTGCCCGATGCCTGGCTGG
AGCTGCAGATCACCGAAGGCCGCAACCGGCAGGTGCGGCGCATGACCGCAGCGGTGGGCTTGCCGACCTTGCGCCTGGTG
CGCGTGGCCATCGGCGATTGGCGGCTGGACGCGCTTGCGCCCGGGCAGTGGCGCGCTGAAGCAACAACCAACGCACGGCC
GAGTCGGAGCCGGCGCTGA

Upstream 100 bases:

>100_bases
TCGTCAAGCGCTGACGCAGACCTGCATGCCGGCGCGTTGCGCGTGTCGGCATGCAGATGTTTGACGGCATCGGGTTCGTG
CAGCGCGTCGCGCAACAGGA

Downstream 100 bases:

>100_bases
ACCGACGCGCACGCGGTGTGCGAACCGACGGCATCAGCACCGGCCGCGCGTGCCTGGCGGCTGCATAGCGCTGTATGAGC
TGATCTCAAGCGCAGAGTGG

Product: pseudouridylate synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 192; Mature: 191

Protein sequence:

>192_residues
MAMLVLLNKPYGVLSQFSDRSQPPKRTLAEFGLPPDVYAAGRLDHDSEGLLVLTDDGALAHRLTDPRHKQPKTYWVQVEG
APQAEHLQALRDGVALNDGPTRPAQVRMLDPAPQLWPRDPPVRVRKTVPDAWLELQITEGRNRQVRRMTAAVGLPTLRLV
RVAIGDWRLDALAPGQWRAEATTNARPSRSRR

Sequences:

>Translated_192_residues
MAMLVLLNKPYGVLSQFSDRSQPPKRTLAEFGLPPDVYAAGRLDHDSEGLLVLTDDGALAHRLTDPRHKQPKTYWVQVEG
APQAEHLQALRDGVALNDGPTRPAQVRMLDPAPQLWPRDPPVRVRKTVPDAWLELQITEGRNRQVRRMTAAVGLPTLRLV
RVAIGDWRLDALAPGQWRAEATTNARPSRSRR
>Mature_191_residues
AMLVLLNKPYGVLSQFSDRSQPPKRTLAEFGLPPDVYAAGRLDHDSEGLLVLTDDGALAHRLTDPRHKQPKTYWVQVEGA
PQAEHLQALRDGVALNDGPTRPAQVRMLDPAPQLWPRDPPVRVRKTVPDAWLELQITEGRNRQVRRMTAAVGLPTLRLVR
VAIGDWRLDALAPGQWRAEATTNARPSRSRR

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=175, Percent_Identity=58.8571428571429, Blast_Score=209, Evalue=1e-55,
Organism=Escherichia coli, GI1788510, Length=176, Percent_Identity=36.9318181818182, Blast_Score=95, Evalue=3e-21,
Organism=Escherichia coli, GI1787524, Length=128, Percent_Identity=36.71875, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1790453, Length=177, Percent_Identity=27.683615819209, Blast_Score=64, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 21507; Mature: 21376

Theoretical pI: Translated: 10.91; Mature: 10.91

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMLVLLNKPYGVLSQFSDRSQPPKRTLAEFGLPPDVYAAGRLDHDSEGLLVLTDDGALA
CEEEEEECCCHHHHHHHHCCCCCHHHHHHHCCCCCCCEECCCCCCCCCCEEEEECCCCHH
HRLTDPRHKQPKTYWVQVEGAPQAEHLQALRDGVALNDGPTRPAQVRMLDPAPQLWPRDP
HHCCCCCCCCCCEEEEEEECCCCHHHHHHHHCCEEECCCCCCCEEEEEECCCCCCCCCCC
PVRVRKTVPDAWLELQITEGRNRQVRRMTAAVGLPTLRLVRVAIGDWRLDALAPGQWRAE
CEEEEECCCCCEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHCCEEEEEECCCCEECC
ATTNARPSRSRR
CCCCCCCCCCCC
>Mature Secondary Structure 
AMLVLLNKPYGVLSQFSDRSQPPKRTLAEFGLPPDVYAAGRLDHDSEGLLVLTDDGALA
EEEEEECCCHHHHHHHHCCCCCHHHHHHHCCCCCCCEECCCCCCCCCCEEEEECCCCHH
HRLTDPRHKQPKTYWVQVEGAPQAEHLQALRDGVALNDGPTRPAQVRMLDPAPQLWPRDP
HHCCCCCCCCCCEEEEEEECCCCHHHHHHHHCCEEECCCCCCCEEEEEECCCCCCCCCCC
PVRVRKTVPDAWLELQITEGRNRQVRRMTAAVGLPTLRLVRVAIGDWRLDALAPGQWRAE
CEEEEECCCCCEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHCCEEEEEECCCCEECC
ATTNARPSRSRR
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]