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The map label for this gene is proP [H]

Identifier: 21240884

GI number: 21240884

Start: 127200

End: 128672

Strand: Direct

Name: proP [H]

Synonym: XAC0110

Alternate gene names: 21240884

Gene position: 127200-128672 (Clockwise)

Preceding gene: 21240883

Following gene: 21240886

Centisome position: 2.46

GC content: 65.65

Gene sequence:

>1473_bases
ATGCGTTCGCACTTTGGCTGGTTCAAACGCCGCCGCCAGCTGCAACTGGATGAGGTCACCGTGGTGGACCGCAGCATGCT
GCGCAGGGCCGTCGGCGCTGCGGCGCTCGGCAATGCGATGGAGTGGTTCGACTTCGGCGTGTACGGCTATCTGGCGGTGA
CGCTGGGCCAGGTGTTCTTCCCGGCAAGCAACCCTACCGCACAATTGATCGCGACCTTCGCCACCTTCACCGTGGCCTTT
CTGGTGCGGCCGATCGGCGGCATGGTCTTCGGACCGCTCGGCGACCGCTACGGGCGCCAGAAAGTCCTGGCCGCCACGAT
GATCCTGATGGCGCTGGGCACCTTCAGTATCGGACTGATTCCGGCCTACCAGCGCATTGGCCTGTGGGCGCCTGCACTGC
TGTTGCTGGCGCGCGTGCTGCAGGGATTTTCCACCGGCGGCGAATACGGTGGCGCGGCCACCTTCATTGCCGAATACGCC
ACCGACCGCAATCGCGGCCTGATGGGCAGCTGGCTGGAGTTCGGCACGCTGGGTGGCTACATCGCCGGCGCCGCCACCGT
GACCGCCTTGCACATGAGCGTGACTCCGGCGCAGATGCTCGATTGGGGCTGGCGCGTGCCGTTCCTGATCGCCGGGCCGT
TGGGGCTGCTGGGCCTGTACATGCGCATGAAGCTGGAGGAGACCCCGGCATTTCGCGCCCATACCGAACAATCCGAACAG
CGCGAGCGGCAGACCGCCGCGCAGGGCCTGACCACGCTGCTGCGCCTGCATTGGCCGCAACTGCTCAAGTGCGTGGGCCT
GGTACTGGTGTTCAACGTCACCGACTACATGCTGCTGACCTACATGCCCAGCTACCTCAGCGTCACGATGGGTTACGCCG
AGAGCAAGGGCTTGTTGCTGATCATCCTGGTGATGCTGGTGATGATGCCGCTCAACATCGTCGGTGGCATGTTCAGCGAC
AAACTGGGCCGGCGGCCGATGATCATCGGTGCCTGCATCGCACTGTTTGCGTTGGCGATCCCGTGCCTGTTGCTGGTCGG
CAGCGGCAACGATGGGCTGATCTTCGCCGGACTGATGCTGCTTGGCCTGGCCCTGGTGTGCTTCACCAGCTCGATGCCGT
CCACCTTGCCGGCGCTGTTCTACACGCCGGTGCGCTACAGCGCGTTGTCGATTGCCTTCAACGTTTCGGTGTCGTTGTTT
GGCGGCACCACGCCGTTGGTCACTGCCTGGCTGGTGGAGCGCACCGGCGACCCGCTGGTGCCGGCCTACTACCTGATGGG
CGCAGCGGCGATCGGCTTGTTGACCATGCTGTTCGTGCGCGAAACCGCAGGATTGCCGCTACGCGGCTCGCCGCCGGCGG
TGGCCAGCGATGCCGAAGCGCGCGCCCTGCTGCAGGCCGACAGCCCGGTGACGGTGGATGCGCAGTTGCCCGTCACCACC
GCAGCCTCGATGGGCCAACCGCGCACGGCGTGA

Upstream 100 bases:

>100_bases
GTCCTGTGCGTTGCCAGGGCGAGCGGGTAGCGACGGCCTGCGCCCGGCGAGATATCCCGGGCGCAATTTACGGAGACCAT
AGATGCACGATGCCCGCGCG

Downstream 100 bases:

>100_bases
GTTGTCAGCTTTTCCGCACGACATGCATCCGCGCCACAGGCGCATATCGTGCTGTTGCAGCAACCGGTGACATAACGCAA
GACGCGATGCCGGCGATGGC

Product: proline-betaine transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 490; Mature: 490

Protein sequence:

>490_residues
MRSHFGWFKRRRQLQLDEVTVVDRSMLRRAVGAAALGNAMEWFDFGVYGYLAVTLGQVFFPASNPTAQLIATFATFTVAF
LVRPIGGMVFGPLGDRYGRQKVLAATMILMALGTFSIGLIPAYQRIGLWAPALLLLARVLQGFSTGGEYGGAATFIAEYA
TDRNRGLMGSWLEFGTLGGYIAGAATVTALHMSVTPAQMLDWGWRVPFLIAGPLGLLGLYMRMKLEETPAFRAHTEQSEQ
RERQTAAQGLTTLLRLHWPQLLKCVGLVLVFNVTDYMLLTYMPSYLSVTMGYAESKGLLLIILVMLVMMPLNIVGGMFSD
KLGRRPMIIGACIALFALAIPCLLLVGSGNDGLIFAGLMLLGLALVCFTSSMPSTLPALFYTPVRYSALSIAFNVSVSLF
GGTTPLVTAWLVERTGDPLVPAYYLMGAAAIGLLTMLFVRETAGLPLRGSPPAVASDAEARALLQADSPVTVDAQLPVTT
AASMGQPRTA

Sequences:

>Translated_490_residues
MRSHFGWFKRRRQLQLDEVTVVDRSMLRRAVGAAALGNAMEWFDFGVYGYLAVTLGQVFFPASNPTAQLIATFATFTVAF
LVRPIGGMVFGPLGDRYGRQKVLAATMILMALGTFSIGLIPAYQRIGLWAPALLLLARVLQGFSTGGEYGGAATFIAEYA
TDRNRGLMGSWLEFGTLGGYIAGAATVTALHMSVTPAQMLDWGWRVPFLIAGPLGLLGLYMRMKLEETPAFRAHTEQSEQ
RERQTAAQGLTTLLRLHWPQLLKCVGLVLVFNVTDYMLLTYMPSYLSVTMGYAESKGLLLIILVMLVMMPLNIVGGMFSD
KLGRRPMIIGACIALFALAIPCLLLVGSGNDGLIFAGLMLLGLALVCFTSSMPSTLPALFYTPVRYSALSIAFNVSVSLF
GGTTPLVTAWLVERTGDPLVPAYYLMGAAAIGLLTMLFVRETAGLPLRGSPPAVASDAEARALLQADSPVTVDAQLPVTT
AASMGQPRTA
>Mature_490_residues
MRSHFGWFKRRRQLQLDEVTVVDRSMLRRAVGAAALGNAMEWFDFGVYGYLAVTLGQVFFPASNPTAQLIATFATFTVAF
LVRPIGGMVFGPLGDRYGRQKVLAATMILMALGTFSIGLIPAYQRIGLWAPALLLLARVLQGFSTGGEYGGAATFIAEYA
TDRNRGLMGSWLEFGTLGGYIAGAATVTALHMSVTPAQMLDWGWRVPFLIAGPLGLLGLYMRMKLEETPAFRAHTEQSEQ
RERQTAAQGLTTLLRLHWPQLLKCVGLVLVFNVTDYMLLTYMPSYLSVTMGYAESKGLLLIILVMLVMMPLNIVGGMFSD
KLGRRPMIIGACIALFALAIPCLLLVGSGNDGLIFAGLMLLGLALVCFTSSMPSTLPALFYTPVRYSALSIAFNVSVSLF
GGTTPLVTAWLVERTGDPLVPAYYLMGAAAIGLLTMLFVRETAGLPLRGSPPAVASDAEARALLQADSPVTVDAQLPVTT
AASMGQPRTA

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=460, Percent_Identity=52.3913043478261, Blast_Score=507, Evalue=1e-145,
Organism=Escherichia coli, GI1789941, Length=425, Percent_Identity=35.7647058823529, Blast_Score=191, Evalue=6e-50,
Organism=Escherichia coli, GI1788942, Length=434, Percent_Identity=30.8755760368664, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1788292, Length=436, Percent_Identity=31.8807339449541, Blast_Score=185, Evalue=7e-48,
Organism=Escherichia coli, GI87082231, Length=438, Percent_Identity=23.0593607305936, Blast_Score=79, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 52736; Mature: 52736

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSHFGWFKRRRQLQLDEVTVVDRSMLRRAVGAAALGNAMEWFDFGVYGYLAVTLGQVFF
CCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHC
PASNPTAQLIATFATFTVAFLVRPIGGMVFGPLGDRYGRQKVLAATMILMALGTFSIGLI
CCCCCHHHHHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
PAYQRIGLWAPALLLLARVLQGFSTGGEYGGAATFIAEYATDRNRGLMGSWLEFGTLGGY
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCHH
IAGAATVTALHMSVTPAQMLDWGWRVPFLIAGPLGLLGLYMRMKLEETPAFRAHTEQSEQ
HHHHHHHHHHHCCCCHHHHHCCCCCCCCEEECHHHHHHHHHHHHHCCCCCHHHHCHHHHH
RERQTAAQGLTTLLRLHWPQLLKCVGLVLVFNVTDYMLLTYMPSYLSVTMGYAESKGLLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
IILVMLVMMPLNIVGGMFSDKLGRRPMIIGACIALFALAIPCLLLVGSGNDGLIFAGLML
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHH
LGLALVCFTSSMPSTLPALFYTPVRYSALSIAFNVSVSLFGGTTPLVTAWLVERTGDPLV
HHHHHHHHHCCCCCHHHHHHHCCHHHHHEEEEEEEEEEEECCCHHHHHHHHHHCCCCCCH
PAYYLMGAAAIGLLTMLFVRETAGLPLRGSPPAVASDAEARALLQADSPVTVDAQLPVTT
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEECCCCCHH
AASMGQPRTA
HHCCCCCCCC
>Mature Secondary Structure
MRSHFGWFKRRRQLQLDEVTVVDRSMLRRAVGAAALGNAMEWFDFGVYGYLAVTLGQVFF
CCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHC
PASNPTAQLIATFATFTVAFLVRPIGGMVFGPLGDRYGRQKVLAATMILMALGTFSIGLI
CCCCCHHHHHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
PAYQRIGLWAPALLLLARVLQGFSTGGEYGGAATFIAEYATDRNRGLMGSWLEFGTLGGY
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCHH
IAGAATVTALHMSVTPAQMLDWGWRVPFLIAGPLGLLGLYMRMKLEETPAFRAHTEQSEQ
HHHHHHHHHHHCCCCHHHHHCCCCCCCCEEECHHHHHHHHHHHHHCCCCCHHHHCHHHHH
RERQTAAQGLTTLLRLHWPQLLKCVGLVLVFNVTDYMLLTYMPSYLSVTMGYAESKGLLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
IILVMLVMMPLNIVGGMFSDKLGRRPMIIGACIALFALAIPCLLLVGSGNDGLIFAGLML
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHH
LGLALVCFTSSMPSTLPALFYTPVRYSALSIAFNVSVSLFGGTTPLVTAWLVERTGDPLV
HHHHHHHHHCCCCCHHHHHHHCCHHHHHEEEEEEEEEEEECCCHHHHHHHHHHCCCCCCH
PAYYLMGAAAIGLLTMLFVRETAGLPLRGSPPAVASDAEARALLQADSPVTVDAQLPVTT
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEECCCCCHH
AASMGQPRTA
HHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]