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The map label for this gene is hsdS [H]

Identifier: 21228805

GI number: 21228805

Start: 3230195

End: 3231517

Strand: Reverse

Name: hsdS [H]

Synonym: MM_2703

Alternate gene names: 21228805

Gene position: 3231517-3230195 (Counterclockwise)

Preceding gene: 21228806

Following gene: 21228799

Centisome position: 78.89

GC content: 38.25

Gene sequence:

>1323_bases
ATGCAGCAGAAGAGTGAACTGCCAGAAGGGTGGGCTGAATGTCAAATAAAAGACATAGTGGTAATTAATTATGGGAAGGG
TTTAAAAAAAAGCGATAGGGTAGAAGGTCAGTTTGATGTATTTGGATCAAATGGAATTGTTGGGAAACACAATCAATCTC
TAACAAATGGTCCTACGGTAATAATTGGAAGAAAAGGCAGTGTTGGTGAAATAAATTTATCTTCAGAACCCTGCTGGCCA
ATTGATACAACTTATTATATTGATAATTTTTACGGGATAAATCGCATTTTTTTATATTATTTACTTAAAACTCTAAATCT
TGCAAATTATGATACTTCTACTGCAATTCCGGGAATTAACCGTAATGACATCTATTCACAATTAGTGCCTCTTCCTCCTC
TTTCCGAACAGCACCGCATCGTTTCAGCTATTGAAGCACTTTTCGCCCGTCTGGATGCAACAAATGAGAAACTTGACAGG
GTGCAGGAGATTCTGAAGAAATTCAGAGAATCGGTGCTTGCGGCTGCGTGTGATGGAAGGTTGACCGAAGAATGGAGAAA
AGAAAACTTACACTGTAACGAATATTTTGCTATAGATGAAGATCAATTTAACTTAGTTAAACAGTGGAGAATTCCTACCG
TATGGTCATGGTCTACTTTAGAAGACTCTTGTTCCCATGTTGTAGATTGTCCTCATTCTACGCCAAAGTGGACTGATATT
GGTGTCTACTGTGTTCGGACTTCTGAACTGAAATGTGGACATATTGATTTTAGCAATGCCAAATATGTATCAGAAGCAAC
CTACTTAGAGAGAATTAAGCGTTTAAAACCGCAAGAAGGAGATATATTATATAGTCGTGAAGGAACGGTTGGTATAGCTT
CTCTTGTGCCTTCAAATGTGAAAATTTGCTTAGGACAACGTCTCATGCTATTTAGAACTAAAAATAACTTAATTCCCAGC
TTTTTTGTTAAAGTTTTGAATTCTCCGTATATCTATGATTCTGTCAAGAAAAGTACTATGGGAAGTACTGCTCCACGGTT
TAATGTAGCCGATATTAAGAAATTTCCAACCCCTCTTCCCCCACTCCCCGAACAGCAAGAAATTGTCCGTAGAGTTGATG
CACTCTTTGCTTTTGCAGATTCGATTGAAACAAAAGTTGCAGCGGCAAGAGAAAAAACGGAAAAACTAAGGCAGTCAATT
CTTGCAAAAGCATTTTCCGGTCAACTTGTTGAGACACAGGCTGAGATTGTAAGAAGGGAAGGAAGGGATTATGAGACGGC
AGAGGTTTTGATTGAGAGGATAAAGGAAGAGAGGAAACAATAA

Upstream 100 bases:

>100_bases
GACCTGCCCGATCCTGAAGAGCTTGCAGCAGAGGCAATTGATGAGCTTGAAGCGGCGCTGGGTGAGTTAAATGCTATTCT
TGATTTGATGGGGAATGGGT

Downstream 100 bases:

>100_bases
CTATGTATTTTGAAGCTCAGTTTTCCCTTTATTTATCAGGAAAAGCTGGCTGTGAAGCATCTGATGAAATTGGGATTGAT
ATATTGAAAGTTTGATCTTC

Product: type I restriction-modification system specificity subunit

Products: NA

Alternate protein names: S.EcoBI; Type I restriction enzyme EcoBI specificity protein; S protein [H]

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MQQKSELPEGWAECQIKDIVVINYGKGLKKSDRVEGQFDVFGSNGIVGKHNQSLTNGPTVIIGRKGSVGEINLSSEPCWP
IDTTYYIDNFYGINRIFLYYLLKTLNLANYDTSTAIPGINRNDIYSQLVPLPPLSEQHRIVSAIEALFARLDATNEKLDR
VQEILKKFRESVLAAACDGRLTEEWRKENLHCNEYFAIDEDQFNLVKQWRIPTVWSWSTLEDSCSHVVDCPHSTPKWTDI
GVYCVRTSELKCGHIDFSNAKYVSEATYLERIKRLKPQEGDILYSREGTVGIASLVPSNVKICLGQRLMLFRTKNNLIPS
FFVKVLNSPYIYDSVKKSTMGSTAPRFNVADIKKFPTPLPPLPEQQEIVRRVDALFAFADSIETKVAAAREKTEKLRQSI
LAKAFSGQLVETQAEIVRREGRDYETAEVLIERIKEERKQ

Sequences:

>Translated_440_residues
MQQKSELPEGWAECQIKDIVVINYGKGLKKSDRVEGQFDVFGSNGIVGKHNQSLTNGPTVIIGRKGSVGEINLSSEPCWP
IDTTYYIDNFYGINRIFLYYLLKTLNLANYDTSTAIPGINRNDIYSQLVPLPPLSEQHRIVSAIEALFARLDATNEKLDR
VQEILKKFRESVLAAACDGRLTEEWRKENLHCNEYFAIDEDQFNLVKQWRIPTVWSWSTLEDSCSHVVDCPHSTPKWTDI
GVYCVRTSELKCGHIDFSNAKYVSEATYLERIKRLKPQEGDILYSREGTVGIASLVPSNVKICLGQRLMLFRTKNNLIPS
FFVKVLNSPYIYDSVKKSTMGSTAPRFNVADIKKFPTPLPPLPEQQEIVRRVDALFAFADSIETKVAAAREKTEKLRQSI
LAKAFSGQLVETQAEIVRREGRDYETAEVLIERIKEERKQ
>Mature_440_residues
MQQKSELPEGWAECQIKDIVVINYGKGLKKSDRVEGQFDVFGSNGIVGKHNQSLTNGPTVIIGRKGSVGEINLSSEPCWP
IDTTYYIDNFYGINRIFLYYLLKTLNLANYDTSTAIPGINRNDIYSQLVPLPPLSEQHRIVSAIEALFARLDATNEKLDR
VQEILKKFRESVLAAACDGRLTEEWRKENLHCNEYFAIDEDQFNLVKQWRIPTVWSWSTLEDSCSHVVDCPHSTPKWTDI
GVYCVRTSELKCGHIDFSNAKYVSEATYLERIKRLKPQEGDILYSREGTVGIASLVPSNVKICLGQRLMLFRTKNNLIPS
FFVKVLNSPYIYDSVKKSTMGSTAPRFNVADIKKFPTPLPPLPEQQEIVRRVDALFAFADSIETKVAAAREKTEKLRQSI
LAKAFSGQLVETQAEIVRREGRDYETAEVLIERIKEERKQ

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

Organism=Escherichia coli, GI1790807, Length=479, Percent_Identity=28.39248434238, Blast_Score=151, Evalue=7e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 50070; Mature: 50070

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQKSELPEGWAECQIKDIVVINYGKGLKKSDRVEGQFDVFGSNGIVGKHNQSLTNGPTV
CCCCCCCCCCHHCCEEEEEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEE
IIGRKGSVGEINLSSEPCWPIDTTYYIDNFYGINRIFLYYLLKTLNLANYDTSTAIPGIN
EEECCCCCCEEECCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCC
RNDIYSQLVPLPPLSEQHRIVSAIEALFARLDATNEKLDRVQEILKKFRESVLAAACDGR
CHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
LTEEWRKENLHCNEYFAIDEDQFNLVKQWRIPTVWSWSTLEDSCSHVVDCPHSTPKWTDI
HHHHHHHCCCCCCCEEEECCHHHHHHHHCCCCCEEECCCHHHHHHHHCCCCCCCCCCCCC
GVYCVRTSELKCGHIDFSNAKYVSEATYLERIKRLKPQEGDILYSREGTVGIASLVPSNV
EEEEEEECCCEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEHHHCCCCC
KICLGQRLMLFRTKNNLIPSFFVKVLNSPYIYDSVKKSTMGSTAPRFNVADIKKFPTPLP
EEHHCCEEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHCCCCCC
PLPEQQEIVRRVDALFAFADSIETKVAAAREKTEKLRQSILAKAFSGQLVETQAEIVRRE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHC
GRDYETAEVLIERIKEERKQ
CCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQQKSELPEGWAECQIKDIVVINYGKGLKKSDRVEGQFDVFGSNGIVGKHNQSLTNGPTV
CCCCCCCCCCHHCCEEEEEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEE
IIGRKGSVGEINLSSEPCWPIDTTYYIDNFYGINRIFLYYLLKTLNLANYDTSTAIPGIN
EEECCCCCCEEECCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCC
RNDIYSQLVPLPPLSEQHRIVSAIEALFARLDATNEKLDRVQEILKKFRESVLAAACDGR
CHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
LTEEWRKENLHCNEYFAIDEDQFNLVKQWRIPTVWSWSTLEDSCSHVVDCPHSTPKWTDI
HHHHHHHCCCCCCCEEEECCHHHHHHHHCCCCCEEECCCHHHHHHHHCCCCCCCCCCCCC
GVYCVRTSELKCGHIDFSNAKYVSEATYLERIKRLKPQEGDILYSREGTVGIASLVPSNV
EEEEEEECCCEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEHHHCCCCC
KICLGQRLMLFRTKNNLIPSFFVKVLNSPYIYDSVKKSTMGSTAPRFNVADIKKFPTPLP
EEHHCCEEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHCCCCCC
PLPEQQEIVRRVDALFAFADSIETKVAAAREKTEKLRQSILAKAFSGQLVETQAEIVRRE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHC
GRDYETAEVLIERIKEERKQ
CCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6304321 [H]