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The map label for this gene is trm1

Identifier: 21228630

GI number: 21228630

Start: 3013819

End: 3014985

Strand: Reverse

Name: trm1

Synonym: MM_2528

Alternate gene names: 21228630

Gene position: 3014985-3013819 (Counterclockwise)

Preceding gene: 21228631

Following gene: 21228629

Centisome position: 73.6

GC content: 48.84

Gene sequence:

>1167_bases
ATGATCTGTAGAACTATAGTGGAAGGGACAACAAAGATATCGGTTCCGGTCCCGCCTCCGGATGCAAATTTCCCTCCCTC
GGCAGCGCCGGTGTTCTATAACCCGGAAATGGAGCTTAACCGTGATATTAATGTTGCAGCAACTGCAGCATTTGTGGAGA
GGCTTCTTTCAAAAAAAGACATACTGAGAGAGGAGATACGTTATGTGGATGCTTTTTCGGCATCAGGGATCCGTGGACTG
AGGATTGCAGGAGAAGTAGGGATTCACTCGACCATGAATGACTGGAGCCACGAAGCGTTTGAACTGATAAAGGAAAATAT
TAAAATTAACGGGCTTGAGGAAAAAGCCCAGGCTACTCGCAGGAATGCAAATGTCCTGCTTCATGAGCAGAGGTTTCATA
TTGTTGATGTTGACCCCTTTGGCACTCCTTCGCCTTACCTTGATGCGGCTGCAAGTTCGGCATACAGCATGCTTTCGGTT
ACTGCTACAGACACAGCACCTCTCTGTGGCGCACACCTTAACTCAGGGATCAGAAAATACGCATCCGTGCCACTAAATAC
GGAATACCACAGCGAAATGGGATTGAGGGTACTGCTCGGAGCCTGTGCAAGGGAGCTTGCAAAACACGAAAAAGGCATGT
TGCCCCTGCTCTCCCATGTTACACGCCACTACGTCCGCACTTACCTTGAAGTCCTGCCCGGGTCCAGAAAGGCTGATAAG
ACCCTTAAGTCCATGGGATTCGTGGCTCACTGCCCCAGATGCGGTTTCAGGAAACCCGTCTATGGGCTTGCTGTCCATAT
TGAAAAGGAATGCCCTGAATGCGGAGGATTGACAAAAATCGCAGGCCCTCTCTGGCTCGGACCTTATAGAGAGCCTGAGT
TCTGCAATGAAGTGATTTCCGAACTTGAGGCGCATCCCCTGAATACAAAAGAGAAGGTAAGGAAAATAATTACGTTCTGC
AGGGATGAACTCGACATTCCTATGTTCTACGACCAGCACGTAATCTGCAAAGAACTCGGGGCTTCAGCAACAGGTATAGA
GAGCCTTATAGAGGCGCTCAGGGCAGGCGGGTTTGAAGCTTCGAGAACTCATTTCACAGGGACCTCTTTCAAAACAGATG
CTCCCATAGCCGAAATAAAGAAGATAATCCTGGCACTTTCAGGATGA

Upstream 100 bases:

>100_bases
ATTCACTGAAACCGGCAACTACAGTTTCAGCGTTAGCGGATATCCGAACATGCGAATGACCATCACTGTAAAATAATATC
CCAAAGTATAGCAACTTTAG

Downstream 100 bases:

>100_bases
AGAGTATACTCATTTAAAAAATGGAGACATAAAAACTGTTCCAATAATATATAAACTATTAACGAATAATAAACGCCTGA
GCTCAGATACAGAAATGATC

Product: N(2),N(2)-dimethylguanosine tRNA methyltransferase

Products: NA

Alternate protein names: tRNA 2,2-dimethylguanosine-26 methyltransferase; tRNA(guanine-26,N(2)-N(2)) methyltransferase; tRNA(m(2,2)G26)dimethyltransferase

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MICRTIVEGTTKISVPVPPPDANFPPSAAPVFYNPEMELNRDINVAATAAFVERLLSKKDILREEIRYVDAFSASGIRGL
RIAGEVGIHSTMNDWSHEAFELIKENIKINGLEEKAQATRRNANVLLHEQRFHIVDVDPFGTPSPYLDAAASSAYSMLSV
TATDTAPLCGAHLNSGIRKYASVPLNTEYHSEMGLRVLLGACARELAKHEKGMLPLLSHVTRHYVRTYLEVLPGSRKADK
TLKSMGFVAHCPRCGFRKPVYGLAVHIEKECPECGGLTKIAGPLWLGPYREPEFCNEVISELEAHPLNTKEKVRKIITFC
RDELDIPMFYDQHVICKELGASATGIESLIEALRAGGFEASRTHFTGTSFKTDAPIAEIKKIILALSG

Sequences:

>Translated_388_residues
MICRTIVEGTTKISVPVPPPDANFPPSAAPVFYNPEMELNRDINVAATAAFVERLLSKKDILREEIRYVDAFSASGIRGL
RIAGEVGIHSTMNDWSHEAFELIKENIKINGLEEKAQATRRNANVLLHEQRFHIVDVDPFGTPSPYLDAAASSAYSMLSV
TATDTAPLCGAHLNSGIRKYASVPLNTEYHSEMGLRVLLGACARELAKHEKGMLPLLSHVTRHYVRTYLEVLPGSRKADK
TLKSMGFVAHCPRCGFRKPVYGLAVHIEKECPECGGLTKIAGPLWLGPYREPEFCNEVISELEAHPLNTKEKVRKIITFC
RDELDIPMFYDQHVICKELGASATGIESLIEALRAGGFEASRTHFTGTSFKTDAPIAEIKKIILALSG
>Mature_388_residues
MICRTIVEGTTKISVPVPPPDANFPPSAAPVFYNPEMELNRDINVAATAAFVERLLSKKDILREEIRYVDAFSASGIRGL
RIAGEVGIHSTMNDWSHEAFELIKENIKINGLEEKAQATRRNANVLLHEQRFHIVDVDPFGTPSPYLDAAASSAYSMLSV
TATDTAPLCGAHLNSGIRKYASVPLNTEYHSEMGLRVLLGACARELAKHEKGMLPLLSHVTRHYVRTYLEVLPGSRKADK
TLKSMGFVAHCPRCGFRKPVYGLAVHIEKECPECGGLTKIAGPLWLGPYREPEFCNEVISELEAHPLNTKEKVRKIITFC
RDELDIPMFYDQHVICKELGASATGIESLIEALRAGGFEASRTHFTGTSFKTDAPIAEIKKIILALSG

Specific function: Dimethylates a single guanine residue at position 26 of a number of tRNAs using S-adenosyl-L-methionine as donor of the methyl groups

COG id: COG1867

COG function: function code J; N2,N2-dimethylguanosine tRNA methyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TRM1 family

Homologues:

Organism=Homo sapiens, GI8923219, Length=454, Percent_Identity=26.8722466960352, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI209862871, Length=454, Percent_Identity=26.8722466960352, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI216548445, Length=438, Percent_Identity=26.9406392694064, Blast_Score=162, Evalue=5e-40,
Organism=Homo sapiens, GI163792194, Length=332, Percent_Identity=29.2168674698795, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17564890, Length=400, Percent_Identity=29.25, Blast_Score=156, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6320325, Length=435, Percent_Identity=26.2068965517241, Blast_Score=135, Evalue=1e-32,
Organism=Drosophila melanogaster, GI19921190, Length=427, Percent_Identity=27.4004683840749, Blast_Score=163, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRM1_METMA (Q8PU28)

Other databases:

- EMBL:   AE008384
- RefSeq:   NP_634552.1
- ProteinModelPortal:   Q8PU28
- SMR:   Q8PU28
- GeneID:   1480870
- GenomeReviews:   AE008384_GR
- KEGG:   mma:MM_2528
- NMPDR:   fig|192952.1.peg.2528
- HOGENOM:   HBG577872
- OMA:   DAPVFYN
- ProtClustDB:   PRK04338
- BioCyc:   MMAZ192952:MM2528-MONOMER
- BRENDA:   2.1.1.32
- HAMAP:   MF_00290
- InterPro:   IPR022923
- InterPro:   IPR002905
- PANTHER:   PTHR10631
- TIGRFAMs:   TIGR00308

Pfam domain/function: PF02005 TRM

EC number: =2.1.1.32

Molecular weight: Translated: 42785; Mature: 42785

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MICRTIVEGTTKISVPVPPPDANFPPSAAPVFYNPEMELNRDINVAATAAFVERLLSKKD
CCCCHHHCCCEEEEEECCCCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
ILREEIRYVDAFSASGIRGLRIAGEVGIHSTMNDWSHEAFELIKENIKINGLEEKAQATR
HHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
RNANVLLHEQRFHIVDVDPFGTPSPYLDAAASSAYSMLSVTATDTAPLCGAHLNSGIRKY
CCCCEEEEECCEEEEECCCCCCCCHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHH
ASVPLNTEYHSEMGLRVLLGACARELAKHEKGMLPLLSHVTRHYVRTYLEVLPGSRKADK
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHH
TLKSMGFVAHCPRCGFRKPVYGLAVHIEKECPECGGLTKIAGPLWLGPYREPEFCNEVIS
HHHHCCHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHH
ELEAHPLNTKEKVRKIITFCRDELDIPMFYDQHVICKELGASATGIESLIEALRAGGFEA
HHHCCCCCHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHCCCCCCHHHHHHHHHHCCCCC
SRTHFTGTSFKTDAPIAEIKKIILALSG
CCCEECCCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MICRTIVEGTTKISVPVPPPDANFPPSAAPVFYNPEMELNRDINVAATAAFVERLLSKKD
CCCCHHHCCCEEEEEECCCCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
ILREEIRYVDAFSASGIRGLRIAGEVGIHSTMNDWSHEAFELIKENIKINGLEEKAQATR
HHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
RNANVLLHEQRFHIVDVDPFGTPSPYLDAAASSAYSMLSVTATDTAPLCGAHLNSGIRKY
CCCCEEEEECCEEEEECCCCCCCCHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHH
ASVPLNTEYHSEMGLRVLLGACARELAKHEKGMLPLLSHVTRHYVRTYLEVLPGSRKADK
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHH
TLKSMGFVAHCPRCGFRKPVYGLAVHIEKECPECGGLTKIAGPLWLGPYREPEFCNEVIS
HHHHCCHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHH
ELEAHPLNTKEKVRKIITFCRDELDIPMFYDQHVICKELGASATGIESLIEALRAGGFEA
HHHCCCCCHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHCCCCCCHHHHHHHHHHCCCCC
SRTHFTGTSFKTDAPIAEIKKIILALSG
CCCEECCCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12125824