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The map label for this gene is fadH [C]

Identifier: 21228497

GI number: 21228497

Start: 2856716

End: 2857813

Strand: Reverse

Name: fadH [C]

Synonym: MM_2395

Alternate gene names: 21228497

Gene position: 2857813-2856716 (Counterclockwise)

Preceding gene: 21228498

Following gene: 21228496

Centisome position: 69.76

GC content: 46.81

Gene sequence:

>1098_bases
ATGATTTTTGACCCTACCACAGTCTGTAACATGGAACTTCAAAACCGCTTTGTAAGGTCCGCAACGCACGAGTTTATGGC
AGAAAAAGATGGGACTCCAACTTCCAGGCTCGGAGATCTCTATGAGGAACTTGCAAGAAGTGAAGTCGGGTTGATAGTAA
CCGGCTATTCCTGTATCCATCCATGCGGGTGGAGTGATGCCAACCAGCAGGGAATTTATGATGACCGTTTCATCGAGCCT
TACAGGAGAATTACTGAGAGGGTACATAAATACAGGAGCAGGATAGTGCTCCAGATAGTGCATGGAGGGAGGCAGGCAGA
CGTTTCCCAGAAAAATCCTGTCCCCATAGCTCCCTCGGCGGTCAAAGACGGGCATTCTGCAACTGTCCCGAAAGAAATGA
CAGAAGAAGAAATCCTGATAGTAATAGAGGCATTCACACAGGCAGCTGTAAGGGCAAAAAAGGCAGGCTTTGACGGGGTT
CAGCTTCACTGTGCCCATGGCTTCCTGTTAAGCAACTTCATTTCTCCTTACACAAACAGGCGGACTGACAGGTGGGGAGG
GTCCGTAGAAAATCGGGCAAGAATAGTAACAGAGATCGTCAGGCGCATAAAAGAGGAGACAGGCAGCAACTTTCCCATCT
TTGTCAAAATGAATGCAACAGACGGTTTCCAGCCCGGAACGGCAAAAGCAGAACTTGGGCTTACCATATCTCAGGCTATG
GAAACTGCAAAACTTCTGGAAAAGGCAGGCGTATGTGCAATCGAAGTCAGTGGAGGGATAAGCGAAGCAGGCGGAGTGAC
CATAAAGACTGCGATCAATTCTCCTTCTAAAGAGGCTTACTTTAAAGAATATTCTAAAAACATAAAGAGTGCAGTGAGTA
TTCCTGTTATCCTCGTTGGTGGGATCAGGTCTCTTTCCGTAATGGAAGGGCTTCTTGAGAATGGGTTTGCAGACCTTATT
TCCATGAGCAGGGCATTCATCAGCGAACCGGATCTTATTTTAAAACTTAAATCTGGAGAGGCTGAAAAGGCAAGATGCGT
ATCCTGCAACCTGTGTTTTGACCCGGAAGGAATAAGGTGCAATTTCGAGTTTGATTGA

Upstream 100 bases:

>100_bases
GGCCAGGTTTTGCAGCTAATCCGGGTGGTTCAGAGAAATTGCATCTTACTATCCGGGAATCCGGGATAATTATTTACCTT
TCCCTCGCCTATTTTTTCTC

Downstream 100 bases:

>100_bases
TCCTGAAATCTAGCTATTTTCACTTTACAGGTTTTCTTTTCCTTTTTCTGACCAGATCCTCTTTTGTTTTTCTCTTTCCT
TTTTATATTTTCCCGTTTCG

Product: NADH:flavin oxidoreductase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MIFDPTTVCNMELQNRFVRSATHEFMAEKDGTPTSRLGDLYEELARSEVGLIVTGYSCIHPCGWSDANQQGIYDDRFIEP
YRRITERVHKYRSRIVLQIVHGGRQADVSQKNPVPIAPSAVKDGHSATVPKEMTEEEILIVIEAFTQAAVRAKKAGFDGV
QLHCAHGFLLSNFISPYTNRRTDRWGGSVENRARIVTEIVRRIKEETGSNFPIFVKMNATDGFQPGTAKAELGLTISQAM
ETAKLLEKAGVCAIEVSGGISEAGGVTIKTAINSPSKEAYFKEYSKNIKSAVSIPVILVGGIRSLSVMEGLLENGFADLI
SMSRAFISEPDLILKLKSGEAEKARCVSCNLCFDPEGIRCNFEFD

Sequences:

>Translated_365_residues
MIFDPTTVCNMELQNRFVRSATHEFMAEKDGTPTSRLGDLYEELARSEVGLIVTGYSCIHPCGWSDANQQGIYDDRFIEP
YRRITERVHKYRSRIVLQIVHGGRQADVSQKNPVPIAPSAVKDGHSATVPKEMTEEEILIVIEAFTQAAVRAKKAGFDGV
QLHCAHGFLLSNFISPYTNRRTDRWGGSVENRARIVTEIVRRIKEETGSNFPIFVKMNATDGFQPGTAKAELGLTISQAM
ETAKLLEKAGVCAIEVSGGISEAGGVTIKTAINSPSKEAYFKEYSKNIKSAVSIPVILVGGIRSLSVMEGLLENGFADLI
SMSRAFISEPDLILKLKSGEAEKARCVSCNLCFDPEGIRCNFEFD
>Mature_365_residues
MIFDPTTVCNMELQNRFVRSATHEFMAEKDGTPTSRLGDLYEELARSEVGLIVTGYSCIHPCGWSDANQQGIYDDRFIEP
YRRITERVHKYRSRIVLQIVHGGRQADVSQKNPVPIAPSAVKDGHSATVPKEMTEEEILIVIEAFTQAAVRAKKAGFDGV
QLHCAHGFLLSNFISPYTNRRTDRWGGSVENRARIVTEIVRRIKEETGSNFPIFVKMNATDGFQPGTAKAELGLTISQAM
ETAKLLEKAGVCAIEVSGGISEAGGVTIKTAINSPSKEAYFKEYSKNIKSAVSIPVILVGGIRSLSVMEGLLENGFADLI
SMSRAFISEPDLILKLKSGEAEKARCVSCNLCFDPEGIRCNFEFD

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=356, Percent_Identity=33.4269662921348, Blast_Score=186, Evalue=1e-48,
Organism=Escherichia coli, GI1787939, Length=354, Percent_Identity=29.0960451977401, Blast_Score=135, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17540738, Length=351, Percent_Identity=31.6239316239316, Blast_Score=157, Evalue=9e-39,
Organism=Caenorhabditis elegans, GI17559802, Length=351, Percent_Identity=32.4786324786325, Blast_Score=154, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI17565138, Length=345, Percent_Identity=32.7536231884058, Blast_Score=154, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI17564188, Length=359, Percent_Identity=31.7548746518106, Blast_Score=152, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI72001454, Length=360, Percent_Identity=30.8333333333333, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17559804, Length=359, Percent_Identity=30.6406685236769, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17566914, Length=346, Percent_Identity=28.0346820809249, Blast_Score=114, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17566916, Length=330, Percent_Identity=26.0606060606061, Blast_Score=78, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6321973, Length=359, Percent_Identity=25.9052924791086, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6325086, Length=359, Percent_Identity=25.9052924791086, Blast_Score=97, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 40178; Mature: 40178

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFDPTTVCNMELQNRFVRSATHEFMAEKDGTPTSRLGDLYEELARSEVGLIVTGYSCIH
CCCCCCCEECHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEECHHHHC
PCGWSDANQQGIYDDRFIEPYRRITERVHKYRSRIVLQIVHGGRQADVSQKNPVPIAPSA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEHEECCCCCCCCCCCCCCCCCCHH
VKDGHSATVPKEMTEEEILIVIEAFTQAAVRAKKAGFDGVQLHCAHGFLLSNFISPYTNR
HCCCCCCCCCHHCCCCCEEEHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHCHHHCC
RTDRWGGSVENRARIVTEIVRRIKEETGSNFPIFVKMNATDGFQPGTAKAELGLTISQAM
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHCCHHHHHH
ETAKLLEKAGVCAIEVSGGISEAGGVTIKTAINSPSKEAYFKEYSKNIKSAVSIPVILVG
HHHHHHHHCCCEEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEC
GIRSLSVMEGLLENGFADLISMSRAFISEPDLILKLKSGEAEKARCVSCNLCFDPEGIRC
CCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEEEECCCCCEE
NFEFD
EEECC
>Mature Secondary Structure
MIFDPTTVCNMELQNRFVRSATHEFMAEKDGTPTSRLGDLYEELARSEVGLIVTGYSCIH
CCCCCCCEECHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEECHHHHC
PCGWSDANQQGIYDDRFIEPYRRITERVHKYRSRIVLQIVHGGRQADVSQKNPVPIAPSA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEHEECCCCCCCCCCCCCCCCCCHH
VKDGHSATVPKEMTEEEILIVIEAFTQAAVRAKKAGFDGVQLHCAHGFLLSNFISPYTNR
HCCCCCCCCCHHCCCCCEEEHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHCHHHCC
RTDRWGGSVENRARIVTEIVRRIKEETGSNFPIFVKMNATDGFQPGTAKAELGLTISQAM
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHCCHHHHHH
ETAKLLEKAGVCAIEVSGGISEAGGVTIKTAINSPSKEAYFKEYSKNIKSAVSIPVILVG
HHHHHHHHCCCEEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEC
GIRSLSVMEGLLENGFADLISMSRAFISEPDLILKLKSGEAEKARCVSCNLCFDPEGIRC
CCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEEEECCCCCEE
NFEFD
EEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]