Click here to switch to the map view.

The map label for this gene is gph [C]

Identifier: 21228451

GI number: 21228451

Start: 2807738

End: 2808532

Strand: Direct

Name: gph [C]

Synonym: MM_2349

Alternate gene names: 21228451

Gene position: 2807738-2808532 (Clockwise)

Preceding gene: 21228448

Following gene: 21228454

Centisome position: 68.54

GC content: 42.77

Gene sequence:

>795_bases
ATGAAACAGATATGTGAAAGTCAAGAAATCATTCGAATGAATGTGCACCAGACCCATCAGAAAGCTCTGAAAGCTATTCT
CTTTGATATGGATAATACTCTTTTTGATTTCGTAGCCGTGAAACTTATCGCTTGCAGGGAGATTCTTTCCTACCTTGGGG
GCGAGGACAGGGATTTTAAGGCAGATCCCGGGGAACTTTTCAGTTATTTTCTCAGGGGGGCTTACGGGTTTGAAGATTAT
GAAAACATAAGGGACTACATGCAGGAGAGAAATCTTTTCACAGATCAGGGGTATAGGGATTGCTGTGAGATTTATGAAAG
AGAAAAACTGGAAAACCTCGAACTGTATCCAGAAGTAAGGGACACACTTGAGGAATTGAAGAAACTTGGTTTCAGGCTTG
TAATTATAACAGATGCCGACAGGCCCCACGCTCGTGCAAGGCTTGCAAAAGTAGACCTTCTTGATTGTTTTGACCTTCTC
GTATCTGCAGATATGACAGGCACAAAGAAACCGGACCCTGCCCATTTTCTCTTTGCTCTTGATGCTCTCGGGATAAAGCC
TGAAGAAAGTCTCGTAGTTGGCGATAGCATAAAAAGGGACATGGCTCCTGCTCGCAGATTGGGCCTGAAAACTGCTTATG
CTTCATACGGAGACTGGAGGCAAGCAGAAGAAACAGAAAGGTGTTTTGACTTCCAGCTAAATGTATTTCAGGACCTGATG
AAATGTATCTATATATTTAATAGGTCTCAGGTTAAAACAGATCCTCCAGGCAAAAACGGACTTAACAGGCATTAA

Upstream 100 bases:

>100_bases
GTACGTAAAAGGAAAAAGGAATGAGAAAAAGATCTGAAAAATCCGGAAAACATAAAATACATTTTAAAAATATTTAATTC
GAAGAAATATGCCACGAAAA

Downstream 100 bases:

>100_bases
AATATCGAAGGATGAACAGTAAAAGTAAAGCAGAAACCAAATAAAATGGTTTTGAAAGGAAAAGCATTTCTTAGAATAAT
ATTTTAAAAACTTCTTGAAG

Product: putative phosphatase

Products: Glycolate; Phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKQICESQEIIRMNVHQTHQKALKAILFDMDNTLFDFVAVKLIACREILSYLGGEDRDFKADPGELFSYFLRGAYGFEDY
ENIRDYMQERNLFTDQGYRDCCEIYEREKLENLELYPEVRDTLEELKKLGFRLVIITDADRPHARARLAKVDLLDCFDLL
VSADMTGTKKPDPAHFLFALDALGIKPEESLVVGDSIKRDMAPARRLGLKTAYASYGDWRQAEETERCFDFQLNVFQDLM
KCIYIFNRSQVKTDPPGKNGLNRH

Sequences:

>Translated_264_residues
MKQICESQEIIRMNVHQTHQKALKAILFDMDNTLFDFVAVKLIACREILSYLGGEDRDFKADPGELFSYFLRGAYGFEDY
ENIRDYMQERNLFTDQGYRDCCEIYEREKLENLELYPEVRDTLEELKKLGFRLVIITDADRPHARARLAKVDLLDCFDLL
VSADMTGTKKPDPAHFLFALDALGIKPEESLVVGDSIKRDMAPARRLGLKTAYASYGDWRQAEETERCFDFQLNVFQDLM
KCIYIFNRSQVKTDPPGKNGLNRH
>Mature_264_residues
MKQICESQEIIRMNVHQTHQKALKAILFDMDNTLFDFVAVKLIACREILSYLGGEDRDFKADPGELFSYFLRGAYGFEDY
ENIRDYMQERNLFTDQGYRDCCEIYEREKLENLELYPEVRDTLEELKKLGFRLVIITDADRPHARARLAKVDLLDCFDLL
VSADMTGTKKPDPAHFLFALDALGIKPEESLVVGDSIKRDMAPARRLGLKTAYASYGDWRQAEETERCFDFQLNVFQDLM
KCIYIFNRSQVKTDPPGKNGLNRH

Specific function: Unknown

COG id: COG1011

COG function: function code R; Predicted hydrolase (HAD superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=217, Percent_Identity=24.8847926267281, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR011950
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: 3.1.3.18 [C]

Molecular weight: Translated: 30680; Mature: 30680

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQICESQEIIRMNVHQTHQKALKAILFDMDNTLFDFVAVKLIACREILSYLGGEDRDFK
CCCHHCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
ADPGELFSYFLRGAYGFEDYENIRDYMQERNLFTDQGYRDCCEIYEREKLENLELYPEVR
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHH
DTLEELKKLGFRLVIITDADRPHARARLAKVDLLDCFDLLVSADMTGTKKPDPAHFLFAL
HHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
DALGIKPEESLVVGDSIKRDMAPARRLGLKTAYASYGDWRQAEETERCFDFQLNVFQDLM
HHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
KCIYIFNRSQVKTDPPGKNGLNRH
HHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKQICESQEIIRMNVHQTHQKALKAILFDMDNTLFDFVAVKLIACREILSYLGGEDRDFK
CCCHHCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
ADPGELFSYFLRGAYGFEDYENIRDYMQERNLFTDQGYRDCCEIYEREKLENLELYPEVR
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHH
DTLEELKKLGFRLVIITDADRPHARARLAKVDLLDCFDLLVSADMTGTKKPDPAHFLFAL
HHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
DALGIKPEESLVVGDSIKRDMAPARRLGLKTAYASYGDWRQAEETERCFDFQLNVFQDLM
HHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
KCIYIFNRSQVKTDPPGKNGLNRH
HHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.57 {phosphoglycolate}} [C]

Substrates: D-Glycerate 2-phosphatelyc; H2O [C]

Specific reaction: D-Glycerate 2-phosphatelyc + H2O --> Glycolate + Phosphate [C]

General reaction: Additional information:

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9679194 [H]