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The map label for this gene is echA [H]

Identifier: 21228422

GI number: 21228422

Start: 2778411

End: 2780327

Strand: Direct

Name: echA [H]

Synonym: MM_2320

Alternate gene names: 21228422

Gene position: 2778411-2780327 (Clockwise)

Preceding gene: 21228421

Following gene: 21228423

Centisome position: 67.83

GC content: 41.99

Gene sequence:

>1917_bases
ATGATAGAAACAACAGTAATGCTATTAATCGCAGTACCCCTACTTTTTTCGTTTTTATTCCTGGGTCTTGGCAAAAGTAT
GTACAAAGTTCTTGCCTGGGCTTTCTTTGTATTTGGGGTAATTGCTTCAGTTCAGTTAGTTCGGGATGGTGTTGGTGTTT
ATGAAATAACTGGACCAGATTTTGCAATGTTAGAAAATGTTGTCCTTGTACTCGAAGTACTGGTTATACTATTCATTCTT
GCTGTGTCAGCAAAATACAAAAACTGGCCTACACTTGTACTCGGAATAATTTCAGCAGCTGTATTTGCCTACACTTTTAC
TAATGCTCCGCATGCTGAAAGCGCTGTTTTTAATATTGATCAATTTTCTCAGGTAATGATATTAATTGTCAATATAGTCG
GTACTGCGATCATACTATTCGCAACCGGTTATATGGATGAATATGAAGAACACAGGCATTTAAACAGACAGAGAACATTC
TATTTCACAATGAGTTTCTTCCTGGCATCCATGAACGGTCTTGTCATGGCTGACACTCTCGGATGGCTGTTTCTCTTCTG
GGAACTGACAACTCTTTGTTCATTCGTCTTGATCTCATACAACATGGACCAGGAAGGAATAAACAATGGTTTCAGAGCTC
TGGCTTTAAACCTGGTTGGTGGAATTGCTTTATCTGTAGGCATAATTATTCTAGCTCTAAATTATGATATAGGCACCCTT
TCCGGAATTGGAATTTACGCTGGAACTGATGCAACAGCGATGGCCGCTGTAGCCCTTCCCGTTGCCTTACTCTGTATCGG
AGGCTTTGCGAAATCCGCTCAGATGCCCTTCCAGAGCTGGCTCCTGGGTGCAATGGTAGCTCCAACTCCGGTTTCTGCCT
TACTGCACTCAAGCACAATGGTTAATGCCGGTGTGTTTTTAGTCGTCAAACTTGTACCAGCTTTTGCGGGCTCAAGTCTC
GGTACAGCTATTGCAGTTTACGGAAGCTTTACCTTCGTCATGTGCTCGGCTTTAGCCTTATCCCAGAGAAACGCAAAAAG
AGTTCTCGCTTATTCTACAATTGCAAACCTGGGATTAATTATCGCAAGTGCAGGAATTGGTACACCTCTGGCTGTAGCTG
CTGCAATTATGCTTATCCTGTTCCACGCAATATCCAAAGCTCTTCTCTTCCTCTGCACAGGTGAAATTGAGCACACCATA
GGAAGCAGAGATATTGAAGACATGGCAGGGTTAATTAAAAAGGCTCCATATCTCACAACCCTTGCAGCGCTGGGAATGGT
ATCCATGCTGTTGCCTCCTTTTGGTGTGCTGCTCACAAAATGGGTGTCGATGGAAGCTGCTTCTAACAACCCTATTGTGA
TAGTCTTCCTTATACTCGGAAGTGCACTCACAACAGTGTATTATGCTAAATGGCTCGGAACAATCCTGTCCTCAACCATG
GATAAATATGCAGTTCACAACAAGAGAGCGGAAACATATTTCCCACTTTCAGTCCTCGGGTTTTCAATTGTAGTTTCAAG
CGTTCTTGTGTTTTCAATATATGACTACTTCATCAAACCTCAGGTTGAAATACTGCTCAATACTGCTCCTGCTATTTCAG
GAGAAGCCGGACAATTCACCTCTGAAATAGGGGCATTTGCTTATGCAGCAATATTCGCAGTGCTTGCTCTGGCTATTATC
CTCTATCTCGCTACAAGAAGCATGTTCACTCCACGTATGGCTGGATATTACATGTGTGGAGAAAACAACATGGAAAGGGA
CAAATTAATGTTCAGAAATGGGCTTTGCACCTACGATAAAAGTAATGTCTCCAATATATATCTCCAGAACACTTTTGGAG
AAAATAAACTTACAACGCTTGGATATGCAATTTCCATAATTCTTATTGTAATTGCATTGGCAGGAGGGGTACTATAA

Upstream 100 bases:

>100_bases
AAAGCTTATTGAGTTTGATTTTATTCTTCAAGTGCGTAAGTCCCAACAGTATAGGTCAAAGGTAAATCGATAGGAGGAAA
TAGGTCGGAGGATACGTTGT

Downstream 100 bases:

>100_bases
TGGTAGCTATGAACGATGTTTTGCTAGCTATTCTTGTTCTGATTGGTGCTCCTATCATAGGCTGTTTAGCTTCGGGGATT
GACAGGAAAATCACTGCAAG

Product: ech hydrogenase subunit

Products: NA

Alternate protein names: Mrp complex subunit A; Multiple resistance and pH homeostasis protein A [H]

Number of amino acids: Translated: 638; Mature: 638

Protein sequence:

>638_residues
MIETTVMLLIAVPLLFSFLFLGLGKSMYKVLAWAFFVFGVIASVQLVRDGVGVYEITGPDFAMLENVVLVLEVLVILFIL
AVSAKYKNWPTLVLGIISAAVFAYTFTNAPHAESAVFNIDQFSQVMILIVNIVGTAIILFATGYMDEYEEHRHLNRQRTF
YFTMSFFLASMNGLVMADTLGWLFLFWELTTLCSFVLISYNMDQEGINNGFRALALNLVGGIALSVGIIILALNYDIGTL
SGIGIYAGTDATAMAAVALPVALLCIGGFAKSAQMPFQSWLLGAMVAPTPVSALLHSSTMVNAGVFLVVKLVPAFAGSSL
GTAIAVYGSFTFVMCSALALSQRNAKRVLAYSTIANLGLIIASAGIGTPLAVAAAIMLILFHAISKALLFLCTGEIEHTI
GSRDIEDMAGLIKKAPYLTTLAALGMVSMLLPPFGVLLTKWVSMEAASNNPIVIVFLILGSALTTVYYAKWLGTILSSTM
DKYAVHNKRAETYFPLSVLGFSIVVSSVLVFSIYDYFIKPQVEILLNTAPAISGEAGQFTSEIGAFAYAAIFAVLALAII
LYLATRSMFTPRMAGYYMCGENNMERDKLMFRNGLCTYDKSNVSNIYLQNTFGENKLTTLGYAISIILIVIALAGGVL

Sequences:

>Translated_638_residues
MIETTVMLLIAVPLLFSFLFLGLGKSMYKVLAWAFFVFGVIASVQLVRDGVGVYEITGPDFAMLENVVLVLEVLVILFIL
AVSAKYKNWPTLVLGIISAAVFAYTFTNAPHAESAVFNIDQFSQVMILIVNIVGTAIILFATGYMDEYEEHRHLNRQRTF
YFTMSFFLASMNGLVMADTLGWLFLFWELTTLCSFVLISYNMDQEGINNGFRALALNLVGGIALSVGIIILALNYDIGTL
SGIGIYAGTDATAMAAVALPVALLCIGGFAKSAQMPFQSWLLGAMVAPTPVSALLHSSTMVNAGVFLVVKLVPAFAGSSL
GTAIAVYGSFTFVMCSALALSQRNAKRVLAYSTIANLGLIIASAGIGTPLAVAAAIMLILFHAISKALLFLCTGEIEHTI
GSRDIEDMAGLIKKAPYLTTLAALGMVSMLLPPFGVLLTKWVSMEAASNNPIVIVFLILGSALTTVYYAKWLGTILSSTM
DKYAVHNKRAETYFPLSVLGFSIVVSSVLVFSIYDYFIKPQVEILLNTAPAISGEAGQFTSEIGAFAYAAIFAVLALAII
LYLATRSMFTPRMAGYYMCGENNMERDKLMFRNGLCTYDKSNVSNIYLQNTFGENKLTTLGYAISIILIVIALAGGVL
>Mature_638_residues
MIETTVMLLIAVPLLFSFLFLGLGKSMYKVLAWAFFVFGVIASVQLVRDGVGVYEITGPDFAMLENVVLVLEVLVILFIL
AVSAKYKNWPTLVLGIISAAVFAYTFTNAPHAESAVFNIDQFSQVMILIVNIVGTAIILFATGYMDEYEEHRHLNRQRTF
YFTMSFFLASMNGLVMADTLGWLFLFWELTTLCSFVLISYNMDQEGINNGFRALALNLVGGIALSVGIIILALNYDIGTL
SGIGIYAGTDATAMAAVALPVALLCIGGFAKSAQMPFQSWLLGAMVAPTPVSALLHSSTMVNAGVFLVVKLVPAFAGSSL
GTAIAVYGSFTFVMCSALALSQRNAKRVLAYSTIANLGLIIASAGIGTPLAVAAAIMLILFHAISKALLFLCTGEIEHTI
GSRDIEDMAGLIKKAPYLTTLAALGMVSMLLPPFGVLLTKWVSMEAASNNPIVIVFLILGSALTTVYYAKWLGTILSSTM
DKYAVHNKRAETYFPLSVLGFSIVVSSVLVFSIYDYFIKPQVEILLNTAPAISGEAGQFTSEIGAFAYAAIFAVLALAII
LYLATRSMFTPRMAGYYMCGENNMERDKLMFRNGLCTYDKSNVSNIYLQNTFGENKLTTLGYAISIILIVIALAGGVL

Specific function: Mnh complex is a Na(+)Li(+)/H(+) antiporter involved in Na(+) and/or Li(+) excretion and Na(+) resistance. Na(+)/H(+) antiport consumes a transmembrane electrical potential, and is thus inferred to be electrogenic. Does not transport K(+), Ca(2+) or Mg(2+

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit A family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=330, Percent_Identity=33.9393939393939, Blast_Score=137, Evalue=3e-32,
Organism=Escherichia coli, GI1788829, Length=382, Percent_Identity=29.3193717277487, Blast_Score=140, Evalue=4e-34,
Organism=Escherichia coli, GI1788614, Length=307, Percent_Identity=34.5276872964169, Blast_Score=132, Evalue=9e-32,
Organism=Escherichia coli, GI1788827, Length=473, Percent_Identity=24.3128964059197, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI1788831, Length=353, Percent_Identity=26.0623229461756, Blast_Score=102, Evalue=1e-22,
Organism=Escherichia coli, GI1788613, Length=317, Percent_Identity=23.9747634069401, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI2367154, Length=208, Percent_Identity=29.3269230769231, Blast_Score=66, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005663
- InterPro:   IPR001750
- InterPro:   IPR001516 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 68797; Mature: 68797

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIETTVMLLIAVPLLFSFLFLGLGKSMYKVLAWAFFVFGVIASVQLVRDGVGVYEITGPD
CCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
FAMLENVVLVLEVLVILFILAVSAKYKNWPTLVLGIISAAVFAYTFTNAPHAESAVFNID
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHEECHH
QFSQVMILIVNIVGTAIILFATGYMDEYEEHRHLNRQRTFYFTMSFFLASMNGLVMADTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEHHHHH
GWLFLFWELTTLCSFVLISYNMDQEGINNGFRALALNLVGGIALSVGIIILALNYDIGTL
HHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCC
SGIGIYAGTDATAMAAVALPVALLCIGGFAKSAQMPFQSWLLGAMVAPTPVSALLHSSTM
CCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHH
VNAGVFLVVKLVPAFAGSSLGTAIAVYGSFTFVMCSALALSQRNAKRVLAYSTIANLGLI
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
IASAGIGTPLAVAAAIMLILFHAISKALLFLCTGEIEHTIGSRDIEDMAGLIKKAPYLTT
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHCHHHHH
LAALGMVSMLLPPFGVLLTKWVSMEAASNNPIVIVFLILGSALTTVYYAKWLGTILSSTM
HHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
DKYAVHNKRAETYFPLSVLGFSIVVSSVLVFSIYDYFIKPQVEILLNTAPAISGEAGQFT
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCCCCCHHH
SEIGAFAYAAIFAVLALAIILYLATRSMFTPRMAGYYMCGENNMERDKLMFRNGLCTYDK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHCCCCCCCC
SNVSNIYLQNTFGENKLTTLGYAISIILIVIALAGGVL
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIETTVMLLIAVPLLFSFLFLGLGKSMYKVLAWAFFVFGVIASVQLVRDGVGVYEITGPD
CCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
FAMLENVVLVLEVLVILFILAVSAKYKNWPTLVLGIISAAVFAYTFTNAPHAESAVFNID
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHEECHH
QFSQVMILIVNIVGTAIILFATGYMDEYEEHRHLNRQRTFYFTMSFFLASMNGLVMADTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEHHHHH
GWLFLFWELTTLCSFVLISYNMDQEGINNGFRALALNLVGGIALSVGIIILALNYDIGTL
HHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCC
SGIGIYAGTDATAMAAVALPVALLCIGGFAKSAQMPFQSWLLGAMVAPTPVSALLHSSTM
CCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHH
VNAGVFLVVKLVPAFAGSSLGTAIAVYGSFTFVMCSALALSQRNAKRVLAYSTIANLGLI
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
IASAGIGTPLAVAAAIMLILFHAISKALLFLCTGEIEHTIGSRDIEDMAGLIKKAPYLTT
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHCHHHHH
LAALGMVSMLLPPFGVLLTKWVSMEAASNNPIVIVFLILGSALTTVYYAKWLGTILSSTM
HHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
DKYAVHNKRAETYFPLSVLGFSIVVSSVLVFSIYDYFIKPQVEILLNTAPAISGEAGQFT
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCCCCCHHH
SEIGAFAYAAIFAVLALAIILYLATRSMFTPRMAGYYMCGENNMERDKLMFRNGLCTYDK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHCCCCCCCC
SNVSNIYLQNTFGENKLTTLGYAISIILIVIALAGGVL
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11356194 [H]