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The map label for this gene is gatD

Identifier: 21228408

GI number: 21228408

Start: 2760600

End: 2761877

Strand: Reverse

Name: gatD

Synonym: MM_2306

Alternate gene names: 21228408

Gene position: 2761877-2760600 (Counterclockwise)

Preceding gene: 21228409

Following gene: 21228407

Centisome position: 67.42

GC content: 48.2

Gene sequence:

>1278_bases
ATGGAATTCAAACAGGGCGATCGGGTACGCATTGAAAAGAACGGCACTGTCTATGAAGGCAAAGTAATGCCTTCCATGGA
GGGATATATCACAATAAAAATGAAAAGCGGTTACAACGCCGGTTTTTCCATAGATAAGGTCAGTATAACTCCCCTGGAAA
ATAACGGGGAAGCTGCAAACGGAGGAAATGGGGGAAAAAACGGGCAAAAAGAGCCTGAGCCGGCAAAAGAAAAAGTTTCT
AAACCTGGGCTCCCGAAAGTTTCTATCCTTTCCACAGGAGGGACAATTGCCAGCAAAATAGACTACAGGACAGGTGCGGT
TACATCCCAGTTTACTGCGGACGATATCCTCGCTGCAATTCCTGAATTGAAAGAAATTGCAGACTTTAAAGGGAGGGCAA
TCTCAAGCATCCTTTCCGAAAACATGGACCCTGATTCCTGGCAAAACCTCGCCAGAGCAGTCGTTGAAGAAATCGAAGCC
GGAGCTGATGGAATAATTGTCACTCACGGGACGGACACCATGATGTATTCTGCTGCAGCCCTTTCTTTTATGATAGAAAC
TCCTGTGCCTATCGTTTTTGTAGGTTCCCAGAGAAGCGCAGACCGCCCCAGCAGCGACAATGCCATGAATGCCATCTGTG
CAGCAAGGGTTGCCATAAGCGATATTGCCGAAGTTGTGGTGGTTATGCACGGCACAAGCTCGGATGACTACTGTGAAATC
CATAGAGGGACAAAGGTCAGGAAAATGCATACCTCCCGCAGGGACGCTTTCAAGTCAGTGAATTCTCTTCCGATAGGGAC
AGTGGATTACGATACAGGAGAAATAAAGACATTTATCGAATACACCGGACGCGGAGAAAAGTCCCTGAAATTCAAACCCG
GAATGGAACCGAAGTGCGCCATTGTGAAGTTTACTCCCGGAGCGGACCCCTCAGTCCTTGACTGTTATGTTGACGGCGGC
TATAAGGGGCTTGTCCTTGAAGGTACCGGCCTGGGGCACGTTTCCACAAAATGGATTCCTTTTATCCGGAGAGCTGTGGA
CGCAAAAATGCCTGTAATAGTCACATCCCAGTGCCTTAACGGCAGGGTTTGTGACCGTGTTTACGATACAGGCCGTGACA
TGCTGAAAGCCGGTGCAATCGAAGGAGAAGACACTCTTCCCGAAACAGCCCTTGTAAAGCTTATGTGGGTACTCGGTCAG
ACAGATGAGTTTGAAAAAGCTGTCAGTATGCTCGGAGAGAATCTCAGCGGGGAAATCAATGAGTGCACTCTGAGATAA

Upstream 100 bases:

>100_bases
ACTAAAACTAAACTTTCAGATTATATACTTATTACAATTACCAAACTTACAGATTGTAACTCATTACTATGCTAAATGAT
CATAAGAAAAGGGATTATTC

Downstream 100 bases:

>100_bases
AATTCCCGAAAACTCTAAAAATCATACAAAGAACCCTGAATCGGGTTCATCTTTAAGTTTTTTCATCTTTAAGCTCTTTC
ATCTTTAAGCTCTTTCATCT

Product: glutamyl-tRNA(Gln) amidotransferase subunit D

Products: L-aspartate; NH3

Alternate protein names: Glu-ADT subunit D

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MEFKQGDRVRIEKNGTVYEGKVMPSMEGYITIKMKSGYNAGFSIDKVSITPLENNGEAANGGNGGKNGQKEPEPAKEKVS
KPGLPKVSILSTGGTIASKIDYRTGAVTSQFTADDILAAIPELKEIADFKGRAISSILSENMDPDSWQNLARAVVEEIEA
GADGIIVTHGTDTMMYSAAALSFMIETPVPIVFVGSQRSADRPSSDNAMNAICAARVAISDIAEVVVVMHGTSSDDYCEI
HRGTKVRKMHTSRRDAFKSVNSLPIGTVDYDTGEIKTFIEYTGRGEKSLKFKPGMEPKCAIVKFTPGADPSVLDCYVDGG
YKGLVLEGTGLGHVSTKWIPFIRRAVDAKMPVIVTSQCLNGRVCDRVYDTGRDMLKAGAIEGEDTLPETALVKLMWVLGQ
TDEFEKAVSMLGENLSGEINECTLR

Sequences:

>Translated_425_residues
MEFKQGDRVRIEKNGTVYEGKVMPSMEGYITIKMKSGYNAGFSIDKVSITPLENNGEAANGGNGGKNGQKEPEPAKEKVS
KPGLPKVSILSTGGTIASKIDYRTGAVTSQFTADDILAAIPELKEIADFKGRAISSILSENMDPDSWQNLARAVVEEIEA
GADGIIVTHGTDTMMYSAAALSFMIETPVPIVFVGSQRSADRPSSDNAMNAICAARVAISDIAEVVVVMHGTSSDDYCEI
HRGTKVRKMHTSRRDAFKSVNSLPIGTVDYDTGEIKTFIEYTGRGEKSLKFKPGMEPKCAIVKFTPGADPSVLDCYVDGG
YKGLVLEGTGLGHVSTKWIPFIRRAVDAKMPVIVTSQCLNGRVCDRVYDTGRDMLKAGAIEGEDTLPETALVKLMWVLGQ
TDEFEKAVSMLGENLSGEINECTLR
>Mature_425_residues
MEFKQGDRVRIEKNGTVYEGKVMPSMEGYITIKMKSGYNAGFSIDKVSITPLENNGEAANGGNGGKNGQKEPEPAKEKVS
KPGLPKVSILSTGGTIASKIDYRTGAVTSQFTADDILAAIPELKEIADFKGRAISSILSENMDPDSWQNLARAVVEEIEA
GADGIIVTHGTDTMMYSAAALSFMIETPVPIVFVGSQRSADRPSSDNAMNAICAARVAISDIAEVVVVMHGTSSDDYCEI
HRGTKVRKMHTSRRDAFKSVNSLPIGTVDYDTGEIKTFIEYTGRGEKSLKFKPGMEPKCAIVKFTPGADPSVLDCYVDGG
YKGLVLEGTGLGHVSTKWIPFIRRAVDAKMPVIVTSQCLNGRVCDRVYDTGRDMLKAGAIEGEDTLPETALVKLMWVLGQ
TDEFEKAVSMLGENLSGEINECTLR

Specific function: Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-ph

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family. GatD subfamily

Homologues:

Organism=Homo sapiens, GI122937331, Length=287, Percent_Identity=29.2682926829268, Blast_Score=90, Evalue=4e-18,
Organism=Escherichia coli, GI1788065, Length=343, Percent_Identity=29.7376093294461, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1789327, Length=343, Percent_Identity=29.4460641399417, Blast_Score=101, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI193207374, Length=313, Percent_Identity=29.3929712460064, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI193207372, Length=314, Percent_Identity=29.9363057324841, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17558278, Length=313, Percent_Identity=29.3929712460064, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17558280, Length=313, Percent_Identity=29.3929712460064, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6320527, Length=295, Percent_Identity=29.1525423728814, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6323189, Length=263, Percent_Identity=28.5171102661597, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323187, Length=263, Percent_Identity=28.5171102661597, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323186, Length=263, Percent_Identity=28.5171102661597, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323184, Length=263, Percent_Identity=28.5171102661597, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24639609, Length=296, Percent_Identity=27.7027027027027, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24645530, Length=260, Percent_Identity=28.4615384615385, Blast_Score=98, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATD_METMA (Q8PUM7)

Other databases:

- EMBL:   AE008384
- RefSeq:   NP_634330.1
- ProteinModelPortal:   Q8PUM7
- SMR:   Q8PUM7
- GeneID:   1480648
- GenomeReviews:   AE008384_GR
- KEGG:   mma:MM_2306
- NMPDR:   fig|192952.1.peg.2306
- HOGENOM:   HBG497495
- OMA:   KMKSGYN
- ProtClustDB:   PRK04183
- BioCyc:   MMAZ192952:MM2306-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00586
- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827
- InterPro:   IPR011878
- PANTHER:   PTHR11707
- PRINTS:   PR00139
- SMART:   SM00870
- TIGRFAMs:   TIGR00519
- TIGRFAMs:   TIGR02153

Pfam domain/function: PF00710 Asparaginase; SSF53774 Asp/Glutamnse

EC number: 3.5.1.1

Molecular weight: Translated: 45846; Mature: 45846

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00144 ASN_GLN_ASE_1; PS00917 ASN_GLN_ASE_2

Important sites: ACT_SITE 95-95 ACT_SITE 171-171 ACT_SITE 172-172 ACT_SITE 248-248

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFKQGDRVRIEKNGTVYEGKVMPSMEGYITIKMKSGYNAGFSIDKVSITPLENNGEAAN
CCCCCCCEEEEECCCEEEECEECCCCCCEEEEEEECCCCCCCEEEEEEEEEECCCCCCCC
GGNGGKNGQKEPEPAKEKVSKPGLPKVSILSTGGTIASKIDYRTGAVTSQFTADDILAAI
CCCCCCCCCCCCCHHHHHHCCCCCCEEEEEECCCCEEHEECCCCCCCCCCCCHHHHHHHH
PELKEIADFKGRAISSILSENMDPDSWQNLARAVVEEIEAGADGIIVTHGTDTMMYSAAA
HHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHH
LSFMIETPVPIVFVGSQRSADRPSSDNAMNAICAARVAISDIAEVVVVMHGTSSDDYCEI
HHEEEECCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEEEECCCCCCEEEE
HRGTKVRKMHTSRRDAFKSVNSLPIGTVDYDTGEIKTFIEYTGRGEKSLKFKPGMEPKCA
CCCCCHHHHHHHHHHHHHHHCCCCCCEEECCCCHHEEEEEECCCCCCCEEECCCCCCCEE
IVKFTPGADPSVLDCYVDGGYKGLVLEGTGLGHVSTKWIPFIRRAVDAKMPVIVTSQCLN
EEEECCCCCCCEEEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEHHHCC
GRVCDRVYDTGRDMLKAGAIEGEDTLPETALVKLMWVLGQTDEFEKAVSMLGENLSGEIN
CHHHHHHHHCCHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCC
ECTLR
CEECC
>Mature Secondary Structure
MEFKQGDRVRIEKNGTVYEGKVMPSMEGYITIKMKSGYNAGFSIDKVSITPLENNGEAAN
CCCCCCCEEEEECCCEEEECEECCCCCCEEEEEEECCCCCCCEEEEEEEEEECCCCCCCC
GGNGGKNGQKEPEPAKEKVSKPGLPKVSILSTGGTIASKIDYRTGAVTSQFTADDILAAI
CCCCCCCCCCCCCHHHHHHCCCCCCEEEEEECCCCEEHEECCCCCCCCCCCCHHHHHHHH
PELKEIADFKGRAISSILSENMDPDSWQNLARAVVEEIEAGADGIIVTHGTDTMMYSAAA
HHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHH
LSFMIETPVPIVFVGSQRSADRPSSDNAMNAICAARVAISDIAEVVVVMHGTSSDDYCEI
HHEEEECCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEEEECCCCCCEEEE
HRGTKVRKMHTSRRDAFKSVNSLPIGTVDYDTGEIKTFIEYTGRGEKSLKFKPGMEPKCA
CCCCCHHHHHHHHHHHHHHHCCCCCCEEECCCCHHEEEEEECCCCCCCEEECCCCCCCEE
IVKFTPGADPSVLDCYVDGGYKGLVLEGTGLGHVSTKWIPFIRRAVDAKMPVIVTSQCLN
EEEECCCCCCCEEEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEHHHCC
GRVCDRVYDTGRDMLKAGAIEGEDTLPETALVKLMWVLGQTDEFEKAVSMLGENLSGEIN
CHHHHHHHHCCHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCC
ECTLR
CEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-asparagine; H2O

Specific reaction: L-asparagine + H2O = L-aspartate + NH3

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: 5-Bromo-4-oxo-L-norvaline; Hg2+; NH3 [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12125824