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The map label for this gene is glnH [H]

Identifier: 21228041

GI number: 21228041

Start: 2307683

End: 2308537

Strand: Direct

Name: glnH [H]

Synonym: MM_1939

Alternate gene names: 21228041

Gene position: 2307683-2308537 (Clockwise)

Preceding gene: 21228040

Following gene: 21228042

Centisome position: 56.34

GC content: 49.36

Gene sequence:

>855_bases
ATGAAAAAAATGTTAAAGATCATGGCAATGCTTTTTGTGATTGCTACTGTTGTCTTTGCAGCCGGCTGTGCTGAACAGGG
AGGAGAGGAAACAGTAGAGGAAGCTGCTGAAGAAAGCGCTCCCGTTGAAGAGACAGCCAGCGCTGAAGTGAATGCTTCCG
GCGAAGAAATGCCCACCTATATCGTAGGTACTGAAGCCCAGTTCCCCCCCTTTGAGATTGTAGACTCCCGCGGGAAAGTG
GTCGGCTTTGACGTTGACCTCCTGAACGCAATTGCTGAAGATCAGGGCTTTAAAGTCCAGTATCTTGACCAGGACTTTGC
AGGCCTCATCCCCGCCCTTCAGACAGGAAACATTGACATCATTGCTTCAGGCATGACAATTACCGAGGAACGCGAACAGG
AAGTCGATTTCAGCGAGCCTTATATTACCGCAGGCCTGGCTCTTGCCGTACCCATTAACAATGAAGAGATCCAGAGTGTT
GCTGATCTTCAGGGCAAGACCGTAGCTGTCCAGACTGGATCCACCGGTTTCATGAAAGCAGAAGAACTCAAAGAAGCCGG
AGTCATTGCTGAGATCAAAGACTTCCCTCACGTCAATGAAGCCATCGAAGAACTCAAGATTGGCGGCGCGGATGTAATGA
TCAATGATCTTCCAGTGACAGAAGCCTTTATTGCAGCCCAGCCAGACGTGATTAAGATTGTGGGAGAGCCTCTCAACAGC
GAGTCCTATGGGTTTGCTGTCCGCACAGGCAACACAGAGCTCCTTCAGAAGATCAACACCGGCCTTGAAAACGTCAAAGC
AAGCGGCGAGTATGACGAACTTCTGACAAACCTGCCCAAATATATGGAAGAATAA

Upstream 100 bases:

>100_bases
TTTTAAACTCTAAAGCTATTAATCTGTTGTTTTACTATTTATAAAAGTTTAATTATCTTACATAATTAATATTAAGGAAG
AGTTTTCAGAGGTAAAATTA

Downstream 100 bases:

>100_bases
ACTTAACCAGATTTACTTCGAGAGCTGCATAATTTTTCTGCTCTCAATTTCTTTTCTTATTAATTTTGTTTTTTCATTCT
CTCTTTTTTGATATCATCGG

Product: glutamine-binding protein

Products: ADP; phosphate; L-glutamine [Cytoplasm] [C]

Alternate protein names: GlnBP [H]

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MKKMLKIMAMLFVIATVVFAAGCAEQGGEETVEEAAEESAPVEETASAEVNASGEEMPTYIVGTEAQFPPFEIVDSRGKV
VGFDVDLLNAIAEDQGFKVQYLDQDFAGLIPALQTGNIDIIASGMTITEEREQEVDFSEPYITAGLALAVPINNEEIQSV
ADLQGKTVAVQTGSTGFMKAEELKEAGVIAEIKDFPHVNEAIEELKIGGADVMINDLPVTEAFIAAQPDVIKIVGEPLNS
ESYGFAVRTGNTELLQKINTGLENVKASGEYDELLTNLPKYMEE

Sequences:

>Translated_284_residues
MKKMLKIMAMLFVIATVVFAAGCAEQGGEETVEEAAEESAPVEETASAEVNASGEEMPTYIVGTEAQFPPFEIVDSRGKV
VGFDVDLLNAIAEDQGFKVQYLDQDFAGLIPALQTGNIDIIASGMTITEEREQEVDFSEPYITAGLALAVPINNEEIQSV
ADLQGKTVAVQTGSTGFMKAEELKEAGVIAEIKDFPHVNEAIEELKIGGADVMINDLPVTEAFIAAQPDVIKIVGEPLNS
ESYGFAVRTGNTELLQKINTGLENVKASGEYDELLTNLPKYMEE
>Mature_284_residues
MKKMLKIMAMLFVIATVVFAAGCAEQGGEETVEEAAEESAPVEETASAEVNASGEEMPTYIVGTEAQFPPFEIVDSRGKV
VGFDVDLLNAIAEDQGFKVQYLDQDFAGLIPALQTGNIDIIASGMTITEEREQEVDFSEPYITAGLALAVPINNEEIQSV
ADLQGKTVAVQTGSTGFMKAEELKEAGVIAEIKDFPHVNEAIEELKIGGADVMINDLPVTEAFIAAQPDVIKIVGEPLNS
ESYGFAVRTGNTELLQKINTGLENVKASGEYDELLTNLPKYMEE

Specific function: Involved in a glutamine-transport system glnHPQ [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787031, Length=217, Percent_Identity=39.63133640553, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1787088, Length=220, Percent_Identity=35.9090909090909, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1788228, Length=216, Percent_Identity=26.8518518518519, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787085, Length=234, Percent_Identity=28.6324786324786, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1788649, Length=226, Percent_Identity=30.0884955752212, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788648, Length=222, Percent_Identity=28.8288288288288, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786876, Length=234, Percent_Identity=27.7777777777778, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: 1440 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 460 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 30532; Mature: 30532

Theoretical pI: Translated: 3.78; Mature: 3.78

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKMLKIMAMLFVIATVVFAAGCAEQGGEETVEEAAEESAPVEETASAEVNASGEEMPTY
CHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCEE
IVGTEAQFPPFEIVDSRGKVVGFDVDLLNAIAEDQGFKVQYLDQDFAGLIPALQTGNIDI
EEECCCCCCCHHEECCCCCEEEECHHHHHHHHCCCCCEEEECCCHHHHHHCHHCCCCEEE
IASGMTITEEREQEVDFSEPYITAGLALAVPINNEEIQSVADLQGKTVAVQTGSTGFMKA
EECCCEECCHHHHCCCCCCCEEECCEEEEEECCHHHHHHHHHCCCCEEEEEECCCCCHHH
EELKEAGVIAEIKDFPHVNEAIEELKIGGADVMINDLPVTEAFIAAQPDVIKIVGEPLNS
HHHHHCCCCHHHHCCCCHHHHHHHHHCCCCEEEEECCCCHHHHHHCCCCEEEEECCCCCC
ESYGFAVRTGNTELLQKINTGLENVKASGEYDELLTNLPKYMEE
CCCEEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKMLKIMAMLFVIATVVFAAGCAEQGGEETVEEAAEESAPVEETASAEVNASGEEMPTY
CHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCEE
IVGTEAQFPPFEIVDSRGKVVGFDVDLLNAIAEDQGFKVQYLDQDFAGLIPALQTGNIDI
EEECCCCCCCHHEECCCCCEEEECHHHHHHHHCCCCCEEEECCCHHHHHHCHHCCCCEEE
IASGMTITEEREQEVDFSEPYITAGLALAVPINNEEIQSVADLQGKTVAVQTGSTGFMKA
EECCCEECCHHHHCCCCCCCEEECCEEEEEECCHHHHHHHHHCCCCEEEEEECCCCCHHH
EELKEAGVIAEIKDFPHVNEAIEELKIGGADVMINDLPVTEAFIAAQPDVIKIVGEPLNS
HHHHHCCCCHHHHCCCCHHHHHHHHHCCCCEEEEECCCCHHHHHHCCCCEEEEECCCCCC
ESYGFAVRTGNTELLQKINTGLENVKASGEYDELLTNLPKYMEE
CCCEEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamine [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamine [Periplasm] + H2O = ADP + phosphate + L-glutamine [Cytoplasm] [C]

General reaction: Translocates substrate across the membrane [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]