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The map label for this gene is bar [H]

Identifier: 21228004

GI number: 21228004

Start: 2261603

End: 2262094

Strand: Reverse

Name: bar [H]

Synonym: MM_1902

Alternate gene names: 21228004

Gene position: 2262094-2261603 (Counterclockwise)

Preceding gene: 21228005

Following gene: 21228002

Centisome position: 55.22

GC content: 44.51

Gene sequence:

>492_bases
GTGGGATACAAATTTTCTCCAATCTCTTCCGAAGATAGGGAGGCTGTGATTGACACTTTCAATTACTATATTGAGAACAG
TTTTGCTGCTTATCCTGAGACCAGGGTTCCTTACGAATTCTTCGAGTTCTTTCTTCAGGTTACTTCAGGATACCCGAATG
CTGTTGTAAGAGATGAAAATAAAAACTTCGTGGGCTTTTGTATGCTCCGCCCCTATTCTCCAATGTCTGCATTTTCCGGA
ACAGCCGAGATATCTTATTTCATCAGGCCCGAGTTCACGGGAAAAGGAACTGGTTCCTTAATGCTCGAACACCTCCTTTC
AGGCGCCAGGGAAAAAGGGCTGCACTCTATCCTGGCAAGCATTTCTTCTCTCAATGAAGGAAGCATCCGTTTCCACAGCC
ACCACGGGTTCTCGGAATGTGGAAGATTCAGGGAAGCGGGAAAAAAGAACGGGAAGTTCTTTGATACGGTATGGATGCAA
AAATTACTTTAA

Upstream 100 bases:

>100_bases
GTACTATATAATTTAGAACCGGTAATAGATTAGCAAATCAGAAAAAAGATTACGGACAGGCAAAATAAAATTTTTGAAAC
ATGGAAAACAGGTGAGTAAA

Downstream 100 bases:

>100_bases
TTACCAGACAAAATGATAAAAAAGTAAAAAGCTAATCCCGGCTTGCTTTGAATGTTACAGATCATCATCAGGATTTATAC
AATATAATGAAATTTCGTAA

Product: hypothetical protein

Products: NA

Alternate protein names: PPT N-acetyltransferase; Phosphinothricin-resistance protein [H]

Number of amino acids: Translated: 163; Mature: 162

Protein sequence:

>163_residues
MGYKFSPISSEDREAVIDTFNYYIENSFAAYPETRVPYEFFEFFLQVTSGYPNAVVRDENKNFVGFCMLRPYSPMSAFSG
TAEISYFIRPEFTGKGTGSLMLEHLLSGAREKGLHSILASISSLNEGSIRFHSHHGFSECGRFREAGKKNGKFFDTVWMQ
KLL

Sequences:

>Translated_163_residues
MGYKFSPISSEDREAVIDTFNYYIENSFAAYPETRVPYEFFEFFLQVTSGYPNAVVRDENKNFVGFCMLRPYSPMSAFSG
TAEISYFIRPEFTGKGTGSLMLEHLLSGAREKGLHSILASISSLNEGSIRFHSHHGFSECGRFREAGKKNGKFFDTVWMQ
KLL
>Mature_162_residues
GYKFSPISSEDREAVIDTFNYYIENSFAAYPETRVPYEFFEFFLQVTSGYPNAVVRDENKNFVGFCMLRPYSPMSAFSGT
AEISYFIRPEFTGKGTGSLMLEHLLSGAREKGLHSILASISSLNEGSIRFHSHHGFSECGRFREAGKKNGKFFDTVWMQK
LL

Specific function: This enzyme is an effector of phosphinothricin tripeptide (PTT or bialaphos) resistance. Inactivates PTT by transfer of an acetyl group [H]

COG id: COG1247

COG function: function code M; Sortase and related acyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 N-acetyltransferase domain [H]

Homologues:

Organism=Escherichia coli, GI1787719, Length=154, Percent_Identity=27.9220779220779, Blast_Score=73, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000182
- InterPro:   IPR016181 [H]

Pfam domain/function: PF00583 Acetyltransf_1 [H]

EC number: =2.3.1.183 [H]

Molecular weight: Translated: 18551; Mature: 18420

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGYKFSPISSEDREAVIDTFNYYIENSFAAYPETRVPYEFFEFFLQVTSGYPNAVVRDEN
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECC
KNFVGFCMLRPYSPMSAFSGTAEISYFIRPEFTGKGTGSLMLEHLLSGAREKGLHSILAS
CCEEEEEEECCCCCHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ISSLNEGSIRFHSHHGFSECGRFREAGKKNGKFFDTVWMQKLL
HHCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHC
>Mature Secondary Structure 
GYKFSPISSEDREAVIDTFNYYIENSFAAYPETRVPYEFFEFFLQVTSGYPNAVVRDEN
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECC
KNFVGFCMLRPYSPMSAFSGTAEISYFIRPEFTGKGTGSLMLEHLLSGAREKGLHSILAS
CCEEEEEEECCCCCHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ISSLNEGSIRFHSHHGFSECGRFREAGKKNGKFFDTVWMQKLL
HHCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1916276; 12000953; 2160942 [H]