Click here to switch to the map view.

The map label for this gene is kefA [C]

Identifier: 21227992

GI number: 21227992

Start: 2244715

End: 2245707

Strand: Reverse

Name: kefA [C]

Synonym: MM_1890

Alternate gene names: 21227992

Gene position: 2245707-2244715 (Counterclockwise)

Preceding gene: 21227993

Following gene: 21227990

Centisome position: 54.82

GC content: 44.01

Gene sequence:

>993_bases
ATGAAGCGCCTGTTCTGGCCCAGGATCAAAAAGACCAGAACAGAGCTAGATGACAGAATCTTTCATCTGTTTGAGAGTTT
CCTGTTAATAATTATCATCCTTTTTGGCATACAGGCGATGATCCGACTTTTAGAGGAATATCTTGGAATTTATGCAGAAT
TCACGGATGACATTTTCTTTCTGGTATACTGGATCACTGGGGTTTATATCAGCTTCCGCATAATATCGATAGTTTCAAAC
TGGTACCTTTCCAGGGTGCCCCTCAGAGATCGCGAAACTATTGACAAACGTTTAATCTACTCCATACGAAATATCCTTAT
GTTTACAGTATCTTTTCTTGCTTTCCTTGTACTTCTCGATTATTTCGAGATTACCGGAGCCGCGTTGACCGCAATGCTTG
CTGCGCTGGGAATAGGAGGGATTATTGTAGGTCTGGCTGCCCAGAACACTCTTAGCAATATCATCACTGGGATAGTACTT
CTTATCGACCGCCCTTTCAGGATAGGAGACCGAATCCATATTGAGAAACTTGACACATGGGGAGATGTGATCGAGATAGG
ATGGCGATCAACTCGCATCATGACTATGGACAACCGGCTCGTGGCAATCCCGAATTCCATTATCGGTACAGAACTCGTCA
TAAATTATTCAACGCCAGAAAAGATGTTTCGCATTGAGACTGATGTAATAGTATCCTATGGACCGGACGCAGAGTATGTA
AGAGGCCTTATCGTGGATGCCCTGAAAAGTGAGAAATGGGTCATGCAGGAAAGACCTGTGCAGGCTCTACTTTTCGAATT
TACAGAGTCCGGGATAAAGTTCAGGGTCAGGTGCTGGATAGAGGACTATGTGGAAAAGCGAATCTCTGAGGACAGGCTCA
ATACTGCCGTCTATAAAGCCCTTATTGACAAAAAGATTGCCATGCCATCCTCGTATCTGGTCTTCTACTTCGCCAATCCT
GAAGATAACAGGATGGTTTACACGAAGGAGTGA

Upstream 100 bases:

>100_bases
GATAACCACAGATACCCTGATAACAATTGGCAACATCCTCATAAGGATTGTCGCAATTATTGTACTCATAATAAGTGCAA
TAATTGTAAGCAGGCTGATA

Downstream 100 bases:

>100_bases
GTATATCACTCGTTTCCGGGTATCAGGTCAGACCCGGCATCGATGTTATCAATTTCCTGTTGTCGAATATCCTGCTCCCG
AAGCTGCTTTCGAAGCTTCA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MKRLFWPRIKKTRTELDDRIFHLFESFLLIIIILFGIQAMIRLLEEYLGIYAEFTDDIFFLVYWITGVYISFRIISIVSN
WYLSRVPLRDRETIDKRLIYSIRNILMFTVSFLAFLVLLDYFEITGAALTAMLAALGIGGIIVGLAAQNTLSNIITGIVL
LIDRPFRIGDRIHIEKLDTWGDVIEIGWRSTRIMTMDNRLVAIPNSIIGTELVINYSTPEKMFRIETDVIVSYGPDAEYV
RGLIVDALKSEKWVMQERPVQALLFEFTESGIKFRVRCWIEDYVEKRISEDRLNTAVYKALIDKKIAMPSSYLVFYFANP
EDNRMVYTKE

Sequences:

>Translated_330_residues
MKRLFWPRIKKTRTELDDRIFHLFESFLLIIIILFGIQAMIRLLEEYLGIYAEFTDDIFFLVYWITGVYISFRIISIVSN
WYLSRVPLRDRETIDKRLIYSIRNILMFTVSFLAFLVLLDYFEITGAALTAMLAALGIGGIIVGLAAQNTLSNIITGIVL
LIDRPFRIGDRIHIEKLDTWGDVIEIGWRSTRIMTMDNRLVAIPNSIIGTELVINYSTPEKMFRIETDVIVSYGPDAEYV
RGLIVDALKSEKWVMQERPVQALLFEFTESGIKFRVRCWIEDYVEKRISEDRLNTAVYKALIDKKIAMPSSYLVFYFANP
EDNRMVYTKE
>Mature_330_residues
MKRLFWPRIKKTRTELDDRIFHLFESFLLIIIILFGIQAMIRLLEEYLGIYAEFTDDIFFLVYWITGVYISFRIISIVSN
WYLSRVPLRDRETIDKRLIYSIRNILMFTVSFLAFLVLLDYFEITGAALTAMLAALGIGGIIVGLAAQNTLSNIITGIVL
LIDRPFRIGDRIHIEKLDTWGDVIEIGWRSTRIMTMDNRLVAIPNSIIGTELVINYSTPEKMFRIETDVIVSYGPDAEYV
RGLIVDALKSEKWVMQERPVQALLFEFTESGIKFRVRCWIEDYVEKRISEDRLNTAVYKALIDKKIAMPSSYLVFYFANP
EDNRMVYTKE

Specific function: Unknown

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1787591, Length=271, Percent_Identity=30.9963099630996, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1786670, Length=265, Percent_Identity=25.2830188679245, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1789291, Length=154, Percent_Identity=33.1168831168831, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI2367355, Length=180, Percent_Identity=26.1111111111111, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014
- InterPro:   IPR008910 [H]

Pfam domain/function: PF00924 MS_channel; PF05552 TM_helix [H]

EC number: NA

Molecular weight: Translated: 38484; Mature: 38484

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLFWPRIKKTRTELDDRIFHLFESFLLIIIILFGIQAMIRLLEEYLGIYAEFTDDIFF
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVYWITGVYISFRIISIVSNWYLSRVPLRDRETIDKRLIYSIRNILMFTVSFLAFLVLLD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YFEITGAALTAMLAALGIGGIIVGLAAQNTLSNIITGIVLLIDRPFRIGDRIHIEKLDTW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCC
GDVIEIGWRSTRIMTMDNRLVAIPNSIIGTELVINYSTPEKMFRIETDVIVSYGPDAEYV
HHHHHCCCCCEEEEEECCEEEECCCCCCCCEEEEEECCHHHHHEEEEEEEEEECCCHHHH
RGLIVDALKSEKWVMQERPVQALLFEFTESGIKFRVRCWIEDYVEKRISEDRLNTAVYKA
HHHHHHHHHCCHHHHHHCCHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHH
LIDKKIAMPSSYLVFYFANPEDNRMVYTKE
HHHHHHCCCCCEEEEEEECCCCCEEEEECC
>Mature Secondary Structure
MKRLFWPRIKKTRTELDDRIFHLFESFLLIIIILFGIQAMIRLLEEYLGIYAEFTDDIFF
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVYWITGVYISFRIISIVSNWYLSRVPLRDRETIDKRLIYSIRNILMFTVSFLAFLVLLD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YFEITGAALTAMLAALGIGGIIVGLAAQNTLSNIITGIVLLIDRPFRIGDRIHIEKLDTW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCC
GDVIEIGWRSTRIMTMDNRLVAIPNSIIGTELVINYSTPEKMFRIETDVIVSYGPDAEYV
HHHHHCCCCCEEEEEECCEEEECCCCCCCCEEEEEECCHHHHHEEEEEEEEEECCCHHHH
RGLIVDALKSEKWVMQERPVQALLFEFTESGIKFRVRCWIEDYVEKRISEDRLNTAVYKA
HHHHHHHHHCCHHHHHHCCHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHH
LIDKKIAMPSSYLVFYFANPEDNRMVYTKE
HHHHHHCCCCCEEEEEEECCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12522265 [H]