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The map label for this gene is nikA [H]

Identifier: 21227961

GI number: 21227961

Start: 2209487

End: 2211268

Strand: Reverse

Name: nikA [H]

Synonym: MM_1859

Alternate gene names: 21227961

Gene position: 2211268-2209487 (Counterclockwise)

Preceding gene: 21227962

Following gene: 21227960

Centisome position: 53.98

GC content: 45.34

Gene sequence:

>1782_bases
ATGAAAAAGAGTAAAGGTTTCAGAGGATTGCAATTAAAGAGAGGGTTAAAAAGCTGGTTGATATTTACCGCACTCATGCT
TGTTTTTTGTTCGGTCATACACCTGGCAGGAGCAGCAGAGCCTGAAAATTCAGGAGAAAGCATTACTCCCTCAAACGACG
GAATTATGGACAGAATTATGACATATATAAAAGGGCTGCTGGGAGAAGAAAATATTCAGGGCTCCGGAGCAGTTTCCGAA
GCAGCAATCTCAGATATTCAGCAGTCGGCAGATTCCGAAAAAGCAGTCCTGAAAATTGCAACTCCGGGAATGGTAAAATC
CACGTCCTTTATAGGGGACTCAAATCTCGGAGTTTTTGCCCATCTCTCAAACCCTCCTCTCATGAAAATGGACTCAGGTG
GGAAACTTACAGGCCAGCTTGTCGAAAATTATGAAGTCTCGGAAAATAACACCTGCTGGACATTTTACCTTAAAAATAAC
CTCTACTGGAGCGACGGAGAACCTGTGACTCCGGAAGACGTTGACTTTTCAATCAGGTACTACGGAAAAAAGACTCCCTG
GGCAAGCTGGATAAACGATACTCTTGAAAGCTCGGCTGTTTCGGACGCAAACAACTCAGTAACCTTCAAATTTAACAAGC
CTTACACCCGCATCGACCTGGAATTTGCCACCTACAATATTCTCCCTGCCCATGTCTGGGAAACAATTGAAAACCCGATG
GAATACACGAATAACGGCCCTTACACAGGCTGCGGGCCTTATTACCTCAAACAGATAGACCTCAATGCCGGGAAGCTTGT
TTTTGAGAAAAACCCTTACTGGAAAGGAAAAGCTCCGGAATTCGAAACTGTGGAGGTCCATTTTTACTCAAGCGCTGACG
TTGCTACCCTTTCCCTTAAAAACGGCGAGGTTGACACCTATTACAAATATGCAGGTTCATACCCCTATTCCGGAATCGAG
CAGCTTGAAGGAACTGGAAATTTCGACTTTATCGAAAAGACGGATATAGGCCTTGTTTTCCTTGCATTCAATCTGGAAAA
AGCACCTTTTTCGGACCCGGAATTCAGAGAAGCTCTGGCTTATGCAATAAATTATGAAGAGATCGTAAACCTTGAGATTC
TGGGTCACGGGGAAGTACCTAACCGCGGTTTTGTGCCACCTGCCATGGAAAACTTCAAGGAAACCGAAAAACTTGAGTAC
AGCCCTGAAAAAGCCAGAAAAACTCTGGAAAAAGCAGGATATAAGGATAGTAATGGAAACGGGATACTTGAAGGAAAAGA
CGGGAAAGACATCAAGCTTGAAATCCTTATCCGGCCGGAGTATGCCCGTACAGGCGAACTTCTTGAAGAATACTTCGAAC
ATGTGGGCCTTGCTTCAGACCTGAGGACTGCGGATTCCGATACCTGGATGACACTCAAGGATAATTACGAGTATGACATG
ACAGTAACCCGCTCCACCCCCTGGGGCATGTTAATGCACGCGAGCTGGGGAAGCGGCTACTTTGATTCCAGAAGGACAGG
GCAGGGAGTTATGCATAACCTGGATGACACCGAATTCCTGAAGCTCTGCGATGATGTCCTCGCAATCACGGATCCCGAAG
AGCTTCAGGCCTATGCATATGAAATTCAGGACTACTATGCAGAAAACCTGCCGGCAGTTCCTCTCTACTGGAACAGTGTT
GTTACCCCGTATAACAGGCACATTGAGGGCTGGTATACCGATCCCCTTTACGGCATTTATAACCTTGATAACTTTGTAAA
TGTGACAAAAATAGATGCATAA

Upstream 100 bases:

>100_bases
GGGAAAAAATAAGTGGAGTAGAAATCTTTTCAAAGTAACCGAACCTCTGGCGCCATCTGTGAAATCAAGGAGAAAAGTCG
AAACAGGAAGAGGCAGCATG

Downstream 100 bases:

>100_bases
AACATAACTGGAGGTATCAAAAAAATTCTGAAAAAGAAATCCTGGAAGTTAAAAAGGTAGAAAAAGAAGTAGAAAGTACG
GCAAAATAGTGCAGGAAATA

Product: dipeptide/oligopeptide binding protein

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 593; Mature: 593

Protein sequence:

>593_residues
MKKSKGFRGLQLKRGLKSWLIFTALMLVFCSVIHLAGAAEPENSGESITPSNDGIMDRIMTYIKGLLGEENIQGSGAVSE
AAISDIQQSADSEKAVLKIATPGMVKSTSFIGDSNLGVFAHLSNPPLMKMDSGGKLTGQLVENYEVSENNTCWTFYLKNN
LYWSDGEPVTPEDVDFSIRYYGKKTPWASWINDTLESSAVSDANNSVTFKFNKPYTRIDLEFATYNILPAHVWETIENPM
EYTNNGPYTGCGPYYLKQIDLNAGKLVFEKNPYWKGKAPEFETVEVHFYSSADVATLSLKNGEVDTYYKYAGSYPYSGIE
QLEGTGNFDFIEKTDIGLVFLAFNLEKAPFSDPEFREALAYAINYEEIVNLEILGHGEVPNRGFVPPAMENFKETEKLEY
SPEKARKTLEKAGYKDSNGNGILEGKDGKDIKLEILIRPEYARTGELLEEYFEHVGLASDLRTADSDTWMTLKDNYEYDM
TVTRSTPWGMLMHASWGSGYFDSRRTGQGVMHNLDDTEFLKLCDDVLAITDPEELQAYAYEIQDYYAENLPAVPLYWNSV
VTPYNRHIEGWYTDPLYGIYNLDNFVNVTKIDA

Sequences:

>Translated_593_residues
MKKSKGFRGLQLKRGLKSWLIFTALMLVFCSVIHLAGAAEPENSGESITPSNDGIMDRIMTYIKGLLGEENIQGSGAVSE
AAISDIQQSADSEKAVLKIATPGMVKSTSFIGDSNLGVFAHLSNPPLMKMDSGGKLTGQLVENYEVSENNTCWTFYLKNN
LYWSDGEPVTPEDVDFSIRYYGKKTPWASWINDTLESSAVSDANNSVTFKFNKPYTRIDLEFATYNILPAHVWETIENPM
EYTNNGPYTGCGPYYLKQIDLNAGKLVFEKNPYWKGKAPEFETVEVHFYSSADVATLSLKNGEVDTYYKYAGSYPYSGIE
QLEGTGNFDFIEKTDIGLVFLAFNLEKAPFSDPEFREALAYAINYEEIVNLEILGHGEVPNRGFVPPAMENFKETEKLEY
SPEKARKTLEKAGYKDSNGNGILEGKDGKDIKLEILIRPEYARTGELLEEYFEHVGLASDLRTADSDTWMTLKDNYEYDM
TVTRSTPWGMLMHASWGSGYFDSRRTGQGVMHNLDDTEFLKLCDDVLAITDPEELQAYAYEIQDYYAENLPAVPLYWNSV
VTPYNRHIEGWYTDPLYGIYNLDNFVNVTKIDA
>Mature_593_residues
MKKSKGFRGLQLKRGLKSWLIFTALMLVFCSVIHLAGAAEPENSGESITPSNDGIMDRIMTYIKGLLGEENIQGSGAVSE
AAISDIQQSADSEKAVLKIATPGMVKSTSFIGDSNLGVFAHLSNPPLMKMDSGGKLTGQLVENYEVSENNTCWTFYLKNN
LYWSDGEPVTPEDVDFSIRYYGKKTPWASWINDTLESSAVSDANNSVTFKFNKPYTRIDLEFATYNILPAHVWETIENPM
EYTNNGPYTGCGPYYLKQIDLNAGKLVFEKNPYWKGKAPEFETVEVHFYSSADVATLSLKNGEVDTYYKYAGSYPYSGIE
QLEGTGNFDFIEKTDIGLVFLAFNLEKAPFSDPEFREALAYAINYEEIVNLEILGHGEVPNRGFVPPAMENFKETEKLEY
SPEKARKTLEKAGYKDSNGNGILEGKDGKDIKLEILIRPEYARTGELLEEYFEHVGLASDLRTADSDTWMTLKDNYEYDM
TVTRSTPWGMLMHASWGSGYFDSRRTGQGVMHNLDDTEFLKLCDDVLAITDPEELQAYAYEIQDYYAENLPAVPLYWNSV
VTPYNRHIEGWYTDPLYGIYNLDNFVNVTKIDA

Specific function: Involved in a nickel transport system, probably represents the nickel binder [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789887, Length=378, Percent_Identity=23.5449735449735, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1787762, Length=460, Percent_Identity=22.1739130434783, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI1787052, Length=471, Percent_Identity=22.5053078556263, Blast_Score=86, Evalue=1e-17,
Organism=Escherichia coli, GI1789966, Length=478, Percent_Identity=21.7573221757322, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI87081878, Length=316, Percent_Identity=24.0506329113924, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI1787551, Length=308, Percent_Identity=25, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1787495, Length=315, Percent_Identity=24.7619047619048, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011980
- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 66827; Mature: 66827

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSKGFRGLQLKRGLKSWLIFTALMLVFCSVIHLAGAAEPENSGESITPSNDGIMDRIM
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHH
TYIKGLLGEENIQGSGAVSEAAISDIQQSADSEKAVLKIATPGMVKSTSFIGDSNLGVFA
HHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCEECCEECCCCCCEEEE
HLSNPPLMKMDSGGKLTGQLVENYEVSENNTCWTFYLKNNLYWSDGEPVTPEDVDFSIRY
EECCCCEEEECCCCCEEHHHHCCEEECCCCEEEEEEEECCEEECCCCCCCCCCCCEEEEE
YGKKTPWASWINDTLESSAVSDANNSVTFKFNKPYTRIDLEFATYNILPAHVWETIENPM
ECCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEEEEEEEEECCHHHHHHHHCCHH
EYTNNGPYTGCGPYYLKQIDLNAGKLVFEKNPYWKGKAPEFETVEVHFYSSADVATLSLK
HHCCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCEEEEEEEECCCCEEEEEEC
NGEVDTYYKYAGSYPYSGIEQLEGTGNFDFIEKTDIGLVFLAFNLEKAPFSDPEFREALA
CCCCCEEEEECCCCCCCCHHHHCCCCCCCEEEECCCEEEEEEEECCCCCCCCHHHHHHHH
YAINYEEIVNLEILGHGEVPNRGFVPPAMENFKETEKLEYSPEKARKTLEKAGYKDSNGN
HHCCHHHEEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCC
GILEGKDGKDIKLEILIRPEYARTGELLEEYFEHVGLASDLRTADSDTWMTLKDNYEYDM
EEEECCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCHHHHCCCCCCCEEEEECCCEEEE
TVTRSTPWGMLMHASWGSGYFDSRRTGQGVMHNLDDTEFLKLCDDVLAITDPEELQAYAY
EEECCCCCCEEEEECCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHH
EIQDYYAENLPAVPLYWNSVVTPYNRHIEGWYTDPLYGIYNLDNFVNVTKIDA
HHHHHHHHCCCCCEEECCCCCCCCCCCCCCEECCCCCEEECCCCCEEEEEECC
>Mature Secondary Structure
MKKSKGFRGLQLKRGLKSWLIFTALMLVFCSVIHLAGAAEPENSGESITPSNDGIMDRIM
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHH
TYIKGLLGEENIQGSGAVSEAAISDIQQSADSEKAVLKIATPGMVKSTSFIGDSNLGVFA
HHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCEECCEECCCCCCEEEE
HLSNPPLMKMDSGGKLTGQLVENYEVSENNTCWTFYLKNNLYWSDGEPVTPEDVDFSIRY
EECCCCEEEECCCCCEEHHHHCCEEECCCCEEEEEEEECCEEECCCCCCCCCCCCEEEEE
YGKKTPWASWINDTLESSAVSDANNSVTFKFNKPYTRIDLEFATYNILPAHVWETIENPM
ECCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEEEEEEEEECCHHHHHHHHCCHH
EYTNNGPYTGCGPYYLKQIDLNAGKLVFEKNPYWKGKAPEFETVEVHFYSSADVATLSLK
HHCCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCEEEEEEEECCCCEEEEEEC
NGEVDTYYKYAGSYPYSGIEQLEGTGNFDFIEKTDIGLVFLAFNLEKAPFSDPEFREALA
CCCCCEEEEECCCCCCCCHHHHCCCCCCCEEEECCCEEEEEEEECCCCCCCCHHHHHHHH
YAINYEEIVNLEILGHGEVPNRGFVPPAMENFKETEKLEYSPEKARKTLEKAGYKDSNGN
HHCCHHHEEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCC
GILEGKDGKDIKLEILIRPEYARTGELLEEYFEHVGLASDLRTADSDTWMTLKDNYEYDM
EEEECCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCHHHHCCCCCCCEEEEECCCEEEE
TVTRSTPWGMLMHASWGSGYFDSRRTGQGVMHNLDDTEFLKLCDDVLAITDPEELQAYAY
EEECCCCCCEEEEECCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHH
EIQDYYAENLPAVPLYWNSVVTPYNRHIEGWYTDPLYGIYNLDNFVNVTKIDA
HHHHHHHHCCCCCEEECCCCCCCCCCCCCCEECCCCCEEECCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7934931; 8041620; 9278503 [H]