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The map label for this gene is rfc [H]

Identifier: 21227923

GI number: 21227923

Start: 2172935

End: 2173951

Strand: Direct

Name: rfc [H]

Synonym: MM_1821

Alternate gene names: 21227923

Gene position: 2172935-2173951 (Clockwise)

Preceding gene: 21227922

Following gene: 21227924

Centisome position: 53.05

GC content: 46.31

Gene sequence:

>1017_bases
GTGCAAGCACAAATGGAGGACTTTAAGATTAAAGAAGAGATCTGGATTGAAAAATACAGGCCTGTCAGGCTTAATCAGGT
AGCAGGACAGGAAGAAACAATCGAGCGCCTGATGTCTTATGTGGCAACTAAAAACCTTCCTCACCTCCTCTTTTCCGGCC
CTCCCGGTGTAGGAAAAACAGCTTCTGCAGTTTCAATAGCAAGGGAGATCTTCGGAGAAGATTTATGGCGGGAAAATTTT
ACGGAACTCAATGCCTCCGATGAAAGGGGTATTGATATTGTCCGAAATAAAATCAAAAACTTTGCAAAAACCGCTCCTAT
GGGCGGAGCCCCTTTCAAAATCATTTTCCTTGATGAAGCTGATGCCCTCACCTCGGATGCCCAGTCCGCACTCCGCAGGA
CGATGGAAAAATTCAGCAGCAATTGCCGTTTTATTCTTTCCTGTAACTATTCCTCCAAGATCATTGAGCCTATCCAGTCC
AGATGTGCGGTTTACAGGTTCAGGAGGCTTTCCGATAAGGCCATCAGAGAACGTCTTGAGTATATTGCTAAAGAACAGGA
CCTGTCCATCACAGATGGTGGGTATGAAGCCCTGATTTATGTAGCTCAGGGGGATATGCGTAAAGCTGTCAACTCTCTCC
AGGCTGCCGCTTTCATTGATGTCGAAAAACCTATCTCCAGGGAAACCATTTACAGGACCACGGCAACTGCGAACCCTGAG
GAGATTAAAAACCTGATTGAAACCGCTTTGCGCGGAAACTTCAGGGTTGCCAGAAAAGAGCTTAACAGGCTTCTTTATGA
AGAAGGGCTTTCCGGAGAAGATATCGTGGGGCAGATATACAGGGTGGTTTCCGAAATGGATAACCTCATGATTCTGGACC
TCGGGCTTTCGGAAAGGGATATTGTCGGACTTGTGGATATTATCGGGGAAACAGACTTCAGACTGACTGAAGGAGCAAGC
GAAAAAATCCAGCTGGAAGCCCTGCTTGCACATTTTGCTCTATCGAGAGAAGAATGA

Upstream 100 bases:

>100_bases
GGGATGGCGTTCTCTTTAGTATTCTTCTTTCGAAATGCGGTCGATATGCCAGAAATATCGAAAACATTCGAAAGTTTAAA
TATTATCTTCTCTAATTAAG

Downstream 100 bases:

>100_bases
AAGTAAGTTATGAAAAAATAAGTTATGAAAAAATAAGTTATGAAAAAATAAGTTGTGAAAAAATAAGTTGTGAAAAAATA
AGTTGTGAAAAAATAAGTTG

Product: replication factor C small subunit

Products: N Pyrophosphate; DNA(N). [C]

Alternate protein names: RFC small subunit; Clamp loader small subunit [H]

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MQAQMEDFKIKEEIWIEKYRPVRLNQVAGQEETIERLMSYVATKNLPHLLFSGPPGVGKTASAVSIAREIFGEDLWRENF
TELNASDERGIDIVRNKIKNFAKTAPMGGAPFKIIFLDEADALTSDAQSALRRTMEKFSSNCRFILSCNYSSKIIEPIQS
RCAVYRFRRLSDKAIRERLEYIAKEQDLSITDGGYEALIYVAQGDMRKAVNSLQAAAFIDVEKPISRETIYRTTATANPE
EIKNLIETALRGNFRVARKELNRLLYEEGLSGEDIVGQIYRVVSEMDNLMILDLGLSERDIVGLVDIIGETDFRLTEGAS
EKIQLEALLAHFALSREE

Sequences:

>Translated_338_residues
MQAQMEDFKIKEEIWIEKYRPVRLNQVAGQEETIERLMSYVATKNLPHLLFSGPPGVGKTASAVSIAREIFGEDLWRENF
TELNASDERGIDIVRNKIKNFAKTAPMGGAPFKIIFLDEADALTSDAQSALRRTMEKFSSNCRFILSCNYSSKIIEPIQS
RCAVYRFRRLSDKAIRERLEYIAKEQDLSITDGGYEALIYVAQGDMRKAVNSLQAAAFIDVEKPISRETIYRTTATANPE
EIKNLIETALRGNFRVARKELNRLLYEEGLSGEDIVGQIYRVVSEMDNLMILDLGLSERDIVGLVDIIGETDFRLTEGAS
EKIQLEALLAHFALSREE
>Mature_338_residues
MQAQMEDFKIKEEIWIEKYRPVRLNQVAGQEETIERLMSYVATKNLPHLLFSGPPGVGKTASAVSIAREIFGEDLWRENF
TELNASDERGIDIVRNKIKNFAKTAPMGGAPFKIIFLDEADALTSDAQSALRRTMEKFSSNCRFILSCNYSSKIIEPIQS
RCAVYRFRRLSDKAIRERLEYIAKEQDLSITDGGYEALIYVAQGDMRKAVNSLQAAAFIDVEKPISRETIYRTTATANPE
EIKNLIETALRGNFRVARKELNRLLYEEGLSGEDIVGQIYRVVSEMDNLMILDLGLSERDIVGLVDIIGETDFRLTEGAS
EKIQLEALLAHFALSREE

Specific function: Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the activator 1 small subunits family. RfcS subfamily [H]

Homologues:

Organism=Homo sapiens, GI31563534, Length=326, Percent_Identity=42.0245398773006, Blast_Score=249, Evalue=2e-66,
Organism=Homo sapiens, GI4506491, Length=332, Percent_Identity=41.8674698795181, Blast_Score=246, Evalue=2e-65,
Organism=Homo sapiens, GI31881687, Length=332, Percent_Identity=41.8674698795181, Blast_Score=246, Evalue=2e-65,
Organism=Homo sapiens, GI6677723, Length=338, Percent_Identity=37.8698224852071, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI194306571, Length=324, Percent_Identity=37.962962962963, Blast_Score=235, Evalue=4e-62,
Organism=Homo sapiens, GI194306567, Length=324, Percent_Identity=37.962962962963, Blast_Score=235, Evalue=4e-62,
Organism=Homo sapiens, GI28882049, Length=323, Percent_Identity=35.6037151702786, Blast_Score=200, Evalue=2e-51,
Organism=Homo sapiens, GI194306569, Length=258, Percent_Identity=39.922480620155, Blast_Score=186, Evalue=2e-47,
Organism=Homo sapiens, GI108773789, Length=231, Percent_Identity=33.3333333333333, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI4506489, Length=231, Percent_Identity=33.3333333333333, Blast_Score=123, Evalue=2e-28,
Organism=Escherichia coli, GI1786676, Length=260, Percent_Identity=25.7692307692308, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71986063, Length=321, Percent_Identity=38.6292834890966, Blast_Score=247, Evalue=5e-66,
Organism=Caenorhabditis elegans, GI17541988, Length=323, Percent_Identity=40.2476780185758, Blast_Score=238, Evalue=3e-63,
Organism=Caenorhabditis elegans, GI17554730, Length=211, Percent_Identity=42.654028436019, Blast_Score=187, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI17541990, Length=227, Percent_Identity=30.8370044052863, Blast_Score=122, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6324478, Length=318, Percent_Identity=39.3081761006289, Blast_Score=237, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6322528, Length=338, Percent_Identity=35.5029585798817, Blast_Score=224, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6324039, Length=328, Percent_Identity=36.890243902439, Blast_Score=221, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6319562, Length=240, Percent_Identity=30.4166666666667, Blast_Score=105, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6324791, Length=233, Percent_Identity=25.7510729613734, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17647857, Length=329, Percent_Identity=41.9452887537994, Blast_Score=251, Evalue=6e-67,
Organism=Drosophila melanogaster, GI19921076, Length=324, Percent_Identity=38.5802469135802, Blast_Score=229, Evalue=2e-60,
Organism=Drosophila melanogaster, GI18859927, Length=322, Percent_Identity=38.1987577639752, Blast_Score=221, Evalue=5e-58,
Organism=Drosophila melanogaster, GI19921136, Length=357, Percent_Identity=27.7310924369748, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003959
- InterPro:   IPR008921
- InterPro:   IPR013748 [H]

Pfam domain/function: PF00004 AAA; PF08542 Rep_fac_C [H]

EC number: 2.7.7.7 [C]

Molecular weight: Translated: 38276; Mature: 38276

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAQMEDFKIKEEIWIEKYRPVRLNQVAGQEETIERLMSYVATKNLPHLLFSGPPGVGKT
CCCCCHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCH
ASAVSIAREIFGEDLWRENFTELNASDERGIDIVRNKIKNFAKTAPMGGAPFKIIFLDEA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCC
DALTSDAQSALRRTMEKFSSNCRFILSCNYSSKIIEPIQSRCAVYRFRRLSDKAIRERLE
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YIAKEQDLSITDGGYEALIYVAQGDMRKAVNSLQAAAFIDVEKPISRETIYRTTATANPE
HHHHHCCCCEECCCCEEEEEEECCHHHHHHHHHHHHHEEECCCCCCHHHHEEECCCCCHH
EIKNLIETALRGNFRVARKELNRLLYEEGLSGEDIVGQIYRVVSEMDNLMILDLGLSERD
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCH
IVGLVDIIGETDFRLTEGASEKIQLEALLAHFALSREE
HHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQAQMEDFKIKEEIWIEKYRPVRLNQVAGQEETIERLMSYVATKNLPHLLFSGPPGVGKT
CCCCCHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCH
ASAVSIAREIFGEDLWRENFTELNASDERGIDIVRNKIKNFAKTAPMGGAPFKIIFLDEA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCC
DALTSDAQSALRRTMEKFSSNCRFILSCNYSSKIIEPIQSRCAVYRFRRLSDKAIRERLE
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YIAKEQDLSITDGGYEALIYVAQGDMRKAVNSLQAAAFIDVEKPISRETIYRTTATANPE
HHHHHCCCCEECCCCEEEEEEECCHHHHHHHHHHHHHEEECCCCCCHHHHEEECCCCCHH
EIKNLIETALRGNFRVARKELNRLLYEEGLSGEDIVGQIYRVVSEMDNLMILDLGLSERD
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCH
IVGLVDIIGETDFRLTEGASEKIQLEALLAHFALSREE
HHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Dimethyl sulfoxide epsilon,; Polymerase alpha accessory factors; Proliferating cell nuclear antigen; Replication factor A; Replication factor C; Thiol [C]

Metal ions: K+; Mg2+; Mn2+; Na+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0063 {dATP}} [C]

Substrates: N Deoxynucleoside Triphosphate; DNA [C]

Specific reaction: N Deoxynucleoside Triphosphate = N Pyrophosphate + DNA(N). Protein + DNA = Protein-DNA [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; 2', 3'-Dideoxythymidine5'-triphosphate polymerase beta, gamma, delta, epsilon; Aphidicolin polymerase alpha; Ara-ATP; Ara-CTP; Benzyl oxycarbonyl -Leu-Leu-al; Carbonyl diphosphonate delta, alpha,; Dideoxynucleoside 5'-triphosphate;

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]