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The map label for this gene is cysG [C]

Identifier: 21227842

GI number: 21227842

Start: 2080921

End: 2081592

Strand: Direct

Name: cysG [C]

Synonym: MM_1740

Alternate gene names: 21227842

Gene position: 2080921-2081592 (Clockwise)

Preceding gene: 21227841

Following gene: 21227843

Centisome position: 50.8

GC content: 46.73

Gene sequence:

>672_bases
ATGGCTGAAACAAACAATTTTCTCCCCCTCATGCTTGACCTTTCAGGCAGGAAAATAGTGATTTTCGGAGGCGGGTCGGT
CGGGGAGCGCAAAGCCGAACTTTTCTGCGGCTGCGCGGACACAGTTGTCGTCAGCCTCGACTTTTCCGAAAGGCTTCAGG
AGCTTGAGGCATCGGGTCAGGTCCGTTTGTCAAAGCTTGACCTTACGGCAGCCACGGACTCCAAACTCAGGGAAATCGTT
TCAGGGGCTTTTCTTGTCATCCCTGCGACCAGCAGCTCCGAGCTTAACCGGAAGATAAGTAATATTGCCGGGGAAAGCGA
TATTTTGATTAATCAGGTGGATGCTTTAGGTAGTGTTGTAATCCCCTCGGTCATCAAAAGAGGAGACCTTGTAATAGGGA
TTTCCACCCTCGGGCATAGCCCTGCGGTCTCTAAATATACCCGTAAACAGATAGAAAATGTGATAACTCCTGCATATTCC
GATATGATCCGGCTTCAGGACGAGCTCAGGAGTTTCCTAAAACTGCATATAAAAGAACAGAAAAAAAGGAAAGTTCTTCT
CTGGAAGGTCCTTGAAAATGAAAAAGTCTGGAACGGCTTTTCCGAATCCTACGAAAAAGCGGCAGAAGAAGCATATAAGA
TAATTTCGGACAATGTCGGTAATTTCGAATAA

Upstream 100 bases:

>100_bases
AACACTATTATTAAACACCAATCTATCATTAAACACCAATCTATCGTTTACCGTAGCTTTCATTTGTTTACCGTAGCTTT
CATTTGCGAGGTCTCTGAAA

Downstream 100 bases:

>100_bases
TATTGAGTAATCAAATAACCTGGAACAATCTCGAATCACCCTGAGTAATCTCGGGCATTCGTTCTTTAATGGAAACCCTT
TAGTGAAAAACGGCTGAGGA

Product: siroheme synthase

Products: sirohydrochlorin; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 223; Mature: 222

Protein sequence:

>223_residues
MAETNNFLPLMLDLSGRKIVIFGGGSVGERKAELFCGCADTVVVSLDFSERLQELEASGQVRLSKLDLTAATDSKLREIV
SGAFLVIPATSSSELNRKISNIAGESDILINQVDALGSVVIPSVIKRGDLVIGISTLGHSPAVSKYTRKQIENVITPAYS
DMIRLQDELRSFLKLHIKEQKKRKVLLWKVLENEKVWNGFSESYEKAAEEAYKIISDNVGNFE

Sequences:

>Translated_223_residues
MAETNNFLPLMLDLSGRKIVIFGGGSVGERKAELFCGCADTVVVSLDFSERLQELEASGQVRLSKLDLTAATDSKLREIV
SGAFLVIPATSSSELNRKISNIAGESDILINQVDALGSVVIPSVIKRGDLVIGISTLGHSPAVSKYTRKQIENVITPAYS
DMIRLQDELRSFLKLHIKEQKKRKVLLWKVLENEKVWNGFSESYEKAAEEAYKIISDNVGNFE
>Mature_222_residues
AETNNFLPLMLDLSGRKIVIFGGGSVGERKAELFCGCADTVVVSLDFSERLQELEASGQVRLSKLDLTAATDSKLREIVS
GAFLVIPATSSSELNRKISNIAGESDILINQVDALGSVVIPSVIKRGDLVIGISTLGHSPAVSKYTRKQIENVITPAYSD
MIRLQDELRSFLKLHIKEQKKRKVLLWKVLENEKVWNGFSESYEKAAEEAYKIISDNVGNFE

Specific function: Multifunctional Enzyme That Catalyze The Sam-Dependent Methylation Of Uroporphyrinogen III At Position C-2 And C-7 To Form Precorrin-2 And Then Position C-12 Or C-18 To Form Trimethylpyrrocorphin 2. It Also Catalyzes The Conversion Of Precorrin-2 Into Sir

COG id: COG1648

COG function: function code H; Siroheme synthase (precorrin-2 oxidase/ferrochelatase domain)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789768, Length=220, Percent_Identity=25.9090909090909, Blast_Score=78, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011128
- InterPro:   IPR016040
- InterPro:   IPR006367 [H]

Pfam domain/function: PF01210 NAD_Gly3P_dh_N [H]

EC number: 1.3.1.76; 4.99.1.4

Molecular weight: Translated: 24590; Mature: 24458

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAETNNFLPLMLDLSGRKIVIFGGGSVGERKAELFCGCADTVVVSLDFSERLQELEASGQ
CCCCCCCEEEEEECCCCEEEEECCCCCCCHHHHHHCCCCCCEEEEECHHHHHHHHHCCCC
VRLSKLDLTAATDSKLREIVSGAFLVIPATSSSELNRKISNIAGESDILINQVDALGSVV
EEEEEEEEECCCHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHH
IPSVIKRGDLVIGISTLGHSPAVSKYTRKQIENVITPAYSDMIRLQDELRSFLKLHIKEQ
HHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
KKRKVLLWKVLENEKVWNGFSESYEKAAEEAYKIISDNVGNFE
HHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AETNNFLPLMLDLSGRKIVIFGGGSVGERKAELFCGCADTVVVSLDFSERLQELEASGQ
CCCCCCEEEEEECCCCEEEEECCCCCCCHHHHHHCCCCCCEEEEECHHHHHHHHHCCCC
VRLSKLDLTAATDSKLREIVSGAFLVIPATSSSELNRKISNIAGESDILINQVDALGSVV
EEEEEEEEECCCHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHH
IPSVIKRGDLVIGISTLGHSPAVSKYTRKQIENVITPAYSDMIRLQDELRSFLKLHIKEQ
HHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
KKRKVLLWKVLENEKVWNGFSESYEKAAEEAYKIISDNVGNFE
HHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Precorrin 2; NAD+

Specific reaction: precorrin-2 + NAD+ = sirohydrochlorin + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]