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The map label for this gene is mttC [H]

Identifier: 21227792

GI number: 21227792

Start: 2032885

End: 2033535

Strand: Direct

Name: mttC [H]

Synonym: MM_1690

Alternate gene names: 21227792

Gene position: 2032885-2033535 (Clockwise)

Preceding gene: 21227791

Following gene: 21227793

Centisome position: 49.63

GC content: 49.62

Gene sequence:

>651_bases
ATGGCAAACAAAGAAGAAATAATTGCAAAAGCAAAAGACGCAATCACTGATTTTGATGATGAACTTGCAGCAGAAGTCGC
AGCAGAAGCTCTCGCTGCAGGGGTTGACCCGGTAGAACTCATTGAGAAAGGCTTTACTGCTGGTATGCAGGAAGTCGGCG
AACAGTTCGAACAGGGTACCCTTTTCCTCCCTCACGTGCTTGCAGCCGCAGAAGCGATGAATGCAGGCATAGAGGTAATT
AAGCCGGAAATGGAAAAGCGCAAGTCTCAGACCAAGAGCCTCGGAACCATTGTCATCGGGACCATCGAAGGCGATATCCA
CTCCATTGGAAAAGACATTGTTGCCTCCATGCTGAATATTGCAGGCTTTAAAGTTGTGGACCTCGGAAGAGATGTCCCGA
TAAAAACCTTCGTTGAAAAGGCAAAGGAAATCAAACCACAGATAATCGCATCCTCCGCCCTTATGACCACAACCATGGTC
AACCAGATCCAGATCGAGGAACAGCTTAAAGAAGCAGGTATCCGCGGCCAGGTCAAGACCATGGTCGGTGGCGCCCCCGT
TACCCAGGACTGGGCTGACAAGATCGGCGCAGACATCTATGGGGAAAGTGCCACAGATGTCGTCAGCAAGGTAAAAGCAG
CCTTACTCTAA

Upstream 100 bases:

>100_bases
TCGATGCAGACATCCTCAAGGACATGAAAGCTGTTGTGGACAAAGCTGACAAAGCTTTCAGAGGCATGTAATTATACCAG
GATATTCAGGAGATCTAAAA

Downstream 100 bases:

>100_bases
AGGCTCAATAAGCAGAAAATGCAGGAGATTTTGTCTAGTGCATTTTCTGTTCAGCTTTATTTTGTACAGTTGCTGCGAAA
CCTGTAGTTGACGGCATAGG

Product: dimethylamine corrinoid protein

Products: L-Methionine; 5,6,7,8-Tetrahydrofolate [C]

Alternate protein names: TCP 1 [H]

Number of amino acids: Translated: 216; Mature: 215

Protein sequence:

>216_residues
MANKEEIIAKAKDAITDFDDELAAEVAAEALAAGVDPVELIEKGFTAGMQEVGEQFEQGTLFLPHVLAAAEAMNAGIEVI
KPEMEKRKSQTKSLGTIVIGTIEGDIHSIGKDIVASMLNIAGFKVVDLGRDVPIKTFVEKAKEIKPQIIASSALMTTTMV
NQIQIEEQLKEAGIRGQVKTMVGGAPVTQDWADKIGADIYGESATDVVSKVKAALL

Sequences:

>Translated_216_residues
MANKEEIIAKAKDAITDFDDELAAEVAAEALAAGVDPVELIEKGFTAGMQEVGEQFEQGTLFLPHVLAAAEAMNAGIEVI
KPEMEKRKSQTKSLGTIVIGTIEGDIHSIGKDIVASMLNIAGFKVVDLGRDVPIKTFVEKAKEIKPQIIASSALMTTTMV
NQIQIEEQLKEAGIRGQVKTMVGGAPVTQDWADKIGADIYGESATDVVSKVKAALL
>Mature_215_residues
ANKEEIIAKAKDAITDFDDELAAEVAAEALAAGVDPVELIEKGFTAGMQEVGEQFEQGTLFLPHVLAAAEAMNAGIEVIK
PEMEKRKSQTKSLGTIVIGTIEGDIHSIGKDIVASMLNIAGFKVVDLGRDVPIKTFVEKAKEIKPQIIASSALMTTTMVN
QIQIEEQLKEAGIRGQVKTMVGGAPVTQDWADKIGADIYGESATDVVSKVKAALL

Specific function: Acts probably as a methyl group carrier between mttB and either mtbA or mtaA [H]

COG id: COG5012

COG function: function code R; Predicted cobalamin binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=164, Percent_Identity=32.9268292682927, Blast_Score=96, Evalue=3e-20,
Organism=Escherichia coli, GI1790450, Length=196, Percent_Identity=31.1224489795918, Blast_Score=105, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17535405, Length=176, Percent_Identity=31.8181818181818, Blast_Score=95, Evalue=3e-20,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR003759
- InterPro:   IPR012741 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2 [H]

EC number: 2.1.1.13 [C]

Molecular weight: Translated: 22950; Mature: 22819

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANKEEIIAKAKDAITDFDDELAAEVAAEALAAGVDPVELIEKGFTAGMQEVGEQFEQGT
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCC
LFLPHVLAAAEAMNAGIEVIKPEMEKRKSQTKSLGTIVIGTIEGDIHSIGKDIVASMLNI
CHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHHHHHHHHH
AGFKVVDLGRDVPIKTFVEKAKEIKPQIIASSALMTTTMVNQIQIEEQLKEAGIRGQVKT
CCCEEEECCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
MVGGAPVTQDWADKIGADIYGESATDVVSKVKAALL
HHCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
ANKEEIIAKAKDAITDFDDELAAEVAAEALAAGVDPVELIEKGFTAGMQEVGEQFEQGT
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCC
LFLPHVLAAAEAMNAGIEVIKPEMEKRKSQTKSLGTIVIGTIEGDIHSIGKDIVASMLNI
CHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHHHHHHHHH
AGFKVVDLGRDVPIKTFVEKAKEIKPQIIASSALMTTTMVNQIQIEEQLKEAGIRGQVKT
CCCEEEECCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
MVGGAPVTQDWADKIGADIYGESATDVVSKVKAALL
HHCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: ATP; Cobinamide; S-adenosyl-L-methionine [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 500-780 [C]

Specific activity: 3.8

Km value (mM): 0.035 {S-methyltetrahydrofolate}} 0.0016 {S-adenosyl-L-methionine}} 0.00065 {S-adenosyl-L-methionine}} 0.06 {methyltetrahydrofolate}} 0.03 {methyltetrahydrofolate}} 0.016 {homocysteine}} [C]

Substrates: 5-Methyltetrahydrofolate; L-Homocysteine [C]

Specific reaction: 5-Methyltetrahydrofolate + L-Homocysteine --> L-Methionine + 5,6,7,8-Tetrahydrofolate [C]

General reaction: Methyl group transfer [C]

Inhibitor: 5, 6-Dimethyl benzimidazole; alpha-Ribazole 3'-phosphate; Be2+; Cobinamide; Propyl iodide; Sr2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]