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The map label for this gene is lacA [C]

Identifier: 21227775

GI number: 21227775

Start: 2005764

End: 2006465

Strand: Reverse

Name: lacA [C]

Synonym: MM_1673

Alternate gene names: 21227775

Gene position: 2006465-2005764 (Counterclockwise)

Preceding gene: 21227776

Following gene: 21227772

Centisome position: 48.98

GC content: 40.6

Gene sequence:

>702_bases
ATGTGTAGTTTTAAGTCCACAGTATTCCACAGTATAACTCTTACTCTTCTTAACGAAGATCTTCTTAAAGAAGAATTAAA
TAAACAGGATTATCATTTTACAAAGGAGGGAACAACTATGGTTGAAATGACTGAAAAAGAAAAAGCAGCTCAGGGCTTAT
TATACAATGCATACTATGATGAAGAGCTGATCTCTCAACGGGCTTATTGCAAAGCAATTTGCTACGAGTATAATCAGCTT
CATCCAGCAAAAATTGAGGAAAGGACAGCTTTAATTCAGAAACTCTTTGGCAAGACCGGAGGTTCTTTTCTTATTGAGCC
GCCATTTATATGCGACTACGGTTACAACATCGAAATAGGCAGCAATTTCTATGCAAATCACAACTGCATCATCCTCGACG
GAGCAAAGGTTGTTTTTGGGGATAATGTGATGATCGCTCCAAACTGCAGCTTCTACACAGCCGGGCATCCGCTTGATGTA
GAACGTCGCAATGCAGGACTGGAATACGCTTATCCAGTAAAAGTCGGCAGCAATGTATGGATAGGCGGCAATGTTTCTGT
ACTTCCCGGAGTAACTATAGGGGATAATGCTGTCATAGGCGCCGGGAGTATTGTGACAAAAGATATTCCTTCCGACGTTG
TTGCTTTTGGGAATCCCTGCAGGGTTATTCGTGAGATTAGTGATGAAGATAAAAAATTTTAA

Upstream 100 bases:

>100_bases
TCGGTTTTGTTTTGATTCCCCTTATTTTCGGACTCAAAGAATGTACTGAGCTTCTTTTCCAATGTTTCTATCTTGCTTTG
AGGCAGGATTTTTCTAAGGG

Downstream 100 bases:

>100_bases
TCCTTTTTTTGCTTGAGCGTTTAAGTTCTATCCAAATAATAAGGAAAACGGAATTCAGAGAGGCTTTTAGAGTTTATAAT
ACGCTTCTGGCTTTTAGAAC

Product: galactoside-O-acetyltransferase

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: NA

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MCSFKSTVFHSITLTLLNEDLLKEELNKQDYHFTKEGTTMVEMTEKEKAAQGLLYNAYYDEELISQRAYCKAICYEYNQL
HPAKIEERTALIQKLFGKTGGSFLIEPPFICDYGYNIEIGSNFYANHNCIILDGAKVVFGDNVMIAPNCSFYTAGHPLDV
ERRNAGLEYAYPVKVGSNVWIGGNVSVLPGVTIGDNAVIGAGSIVTKDIPSDVVAFGNPCRVIREISDEDKKF

Sequences:

>Translated_233_residues
MCSFKSTVFHSITLTLLNEDLLKEELNKQDYHFTKEGTTMVEMTEKEKAAQGLLYNAYYDEELISQRAYCKAICYEYNQL
HPAKIEERTALIQKLFGKTGGSFLIEPPFICDYGYNIEIGSNFYANHNCIILDGAKVVFGDNVMIAPNCSFYTAGHPLDV
ERRNAGLEYAYPVKVGSNVWIGGNVSVLPGVTIGDNAVIGAGSIVTKDIPSDVVAFGNPCRVIREISDEDKKF
>Mature_233_residues
MCSFKSTVFHSITLTLLNEDLLKEELNKQDYHFTKEGTTMVEMTEKEKAAQGLLYNAYYDEELISQRAYCKAICYEYNQL
HPAKIEERTALIQKLFGKTGGSFLIEPPFICDYGYNIEIGSNFYANHNCIILDGAKVVFGDNVMIAPNCSFYTAGHPLDV
ERRNAGLEYAYPVKVGSNVWIGGNVSVLPGVTIGDNAVIGAGSIVTKDIPSDVVAFGNPCRVIREISDEDKKF

Specific function: May Assist Cellular Detoxification By Acetylating Nonmetabolizable Pyranosides, Thereby Preventing Their Reentry Into The Cell. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786537, Length=191, Percent_Identity=51.3089005235602, Blast_Score=197, Evalue=7e-52,
Organism=Escherichia coli, GI1786664, Length=183, Percent_Identity=45.9016393442623, Blast_Score=173, Evalue=1e-44,
Organism=Escherichia coli, GI1788367, Length=145, Percent_Identity=31.0344827586207, Blast_Score=70, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6322243, Length=184, Percent_Identity=59.7826086956522, Blast_Score=233, Evalue=2e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001451
- InterPro:   IPR011004 [H]

Pfam domain/function: PF00132 Hexapep [H]

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 25907; Mature: 25907

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCSFKSTVFHSITLTLLNEDLLKEELNKQDYHFTKEGTTMVEMTEKEKAAQGLLYNAYYD
CCCCHHHHHHHHHEEHHHHHHHHHHCCCCCCEEECCCCEEEEEHHHHHHHCCEEEEEECC
EELISQRAYCKAICYEYNQLHPAKIEERTALIQKLFGKTGGSFLIEPPFICDYGYNIEIG
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCEEECCCCEEEEC
SNFYANHNCIILDGAKVVFGDNVMIAPNCSFYTAGHPLDVERRNAGLEYAYPVKVGSNVW
CCEECCCCEEEEECCEEEECCCEEECCCCCEEECCCCCCCHHHCCCCEEEEEEEECCEEE
IGGNVSVLPGVTIGDNAVIGAGSIVTKDIPSDVVAFGNPCRVIREISDEDKKF
ECCCEEEECCEEECCCCEEECCCEEECCCCCHHHHCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MCSFKSTVFHSITLTLLNEDLLKEELNKQDYHFTKEGTTMVEMTEKEKAAQGLLYNAYYD
CCCCHHHHHHHHHEEHHHHHHHHHHCCCCCCEEECCCCEEEEEHHHHHHHCCEEEEEECC
EELISQRAYCKAICYEYNQLHPAKIEERTALIQKLFGKTGGSFLIEPPFICDYGYNIEIG
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCEEECCCCEEEEC
SNFYANHNCIILDGAKVVFGDNVMIAPNCSFYTAGHPLDVERRNAGLEYAYPVKVGSNVW
CCEECCCCEEEEECCEEEECCCEEECCCCCEEECCCCCCCHHHCCCCEEEEEEEECCEEE
IGGNVSVLPGVTIGDNAVIGAGSIVTKDIPSDVVAFGNPCRVIREISDEDKKF
ECCCEEEECCEEECCCCEEECCCEEECCCCCHHHHCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10708580 [H]