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The map label for this gene is hsdS [H]

Identifier: 21227769

GI number: 21227769

Start: 1995523

End: 1996761

Strand: Direct

Name: hsdS [H]

Synonym: MM_1667

Alternate gene names: 21227769

Gene position: 1995523-1996761 (Clockwise)

Preceding gene: 21227768

Following gene: 21227770

Centisome position: 48.71

GC content: 39.06

Gene sequence:

>1239_bases
TTGCTGGTTGCTTTATATGGTGCTACAATTGGAAAACTTGCGTTTTTGGGTGTTGATGCTGCAACAAATCAAGCTGTATG
TGCAATTTTTAAAAATGGAATTTTTGAATCAAAGTTTTTGTATTATTTGTTTTTCCATAGAAAGCAGGACTTGATTAAAG
AAGCAATTGGTGGGGCACAACCTAACATTAGCCAGACAATTCTTAAAAACTTGGAAGTAACAATTTGCCCTCTTCCCGAA
CAACGCGCCATAGTCTCAAAAATCGAGCAGCTCTTCAGCGAACTGGAAAACGGCATTGCCAACCTCAAACTGGCAAAGGA
ACAGCTTAAAGTATATAGGCAGGCAGTGCTGAAAAAAGCATTTGAAGGGGAGCTTACGAAAAAATGGCGGGAGCAGCAGA
CGGATTTGCCGGATGCAGGGGGGTTGCTGGAGCAAATCAGAAAGGAAAAAGAGAAAGCTGCAAAAAAAGCCGGCAAAAAA
TTGAAACAGGTTAAACCTTTCACTGAGGATGAATTGGAAGATTTGAACAGGTTGCCAAAAGAGTGGAATTGGGTAAAAAT
TGGAAATCTTACACTTGGCGTAGAATATGGAACATCTGCGAAATCAAAAGAATCAGGTGATGTTGCTGTATTGCGCATGG
GAAATATTCAAAACGGAAGGTTTGATTGGAGTGATCTAGTTTATACAAGTGATAAGACCGAAATTGAAAAATATTTATTA
AGTAAAGATGATGTTCTTTTCAATCGTACTAATAGTCCCGAATTAGTTGGTAAAACAGCTATATATAAAGGTGAAAAACC
TGCTATTTTTGCTGGCTATCTAATAAGAATAAATCAACTTTCAGAATTAGCAGTGGCAGATTATTTGAACTACTTTTTGA
ATTGTCATATCGCAAAAGTTCATGGTAATTCCGTAAAAACTGATGGTGTTAATCAATCAAATATAAACGGTGAAAAGCTT
GGGAATTATCCATTTCCTCTCTGTTCTCTTCCCGAACAGCAAACCATTGTTCAGGAAATCGAAACCCGCCTTTCAATCTG
CGATAAGATAGAGCAGGATATTGAAACAAATCTGGAAAAAGCCGAAGCACTCAGGCAGAGTATTTTGAAAAAAGCGTTTG
AGGGGAAGCTGCTTAATGAAAGGGAACTGGCAGAGGTTCGCGGGGCAGAGGATTGGGAGCCGGCTGAGGTTTTGCTGGAA
AGAATAAAAGCTGAAAAGGCGCAGAATGGAAAGAAGTGA

Upstream 100 bases:

>100_bases
GTCGGGTGAACTCAATTATAATACAATTTTTGAAACTGAGGAATATATTTCAGAAGAGGCGATTAAAAAATCAAGTGCAA
AAATCATTCCCAAAGATACA

Downstream 100 bases:

>100_bases
GGAATATGGGTCTGGAAAGGTTGAAAAGGCTGCTTAAAGAGAAGGAAGGTATCCGTCTTGAATTCAAAGAGGCACGGACA
GCTTTACCCGGAAATTTATT

Product: type I restriction-modification system specificity subunit

Products: NA

Alternate protein names: S.EcoKI; Type I restriction enzyme EcoKI specificity protein; S protein [H]

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MLVALYGATIGKLAFLGVDAATNQAVCAIFKNGIFESKFLYYLFFHRKQDLIKEAIGGAQPNISQTILKNLEVTICPLPE
QRAIVSKIEQLFSELENGIANLKLAKEQLKVYRQAVLKKAFEGELTKKWREQQTDLPDAGGLLEQIRKEKEKAAKKAGKK
LKQVKPFTEDELEDLNRLPKEWNWVKIGNLTLGVEYGTSAKSKESGDVAVLRMGNIQNGRFDWSDLVYTSDKTEIEKYLL
SKDDVLFNRTNSPELVGKTAIYKGEKPAIFAGYLIRINQLSELAVADYLNYFLNCHIAKVHGNSVKTDGVNQSNINGEKL
GNYPFPLCSLPEQQTIVQEIETRLSICDKIEQDIETNLEKAEALRQSILKKAFEGKLLNERELAEVRGAEDWEPAEVLLE
RIKAEKAQNGKK

Sequences:

>Translated_412_residues
MLVALYGATIGKLAFLGVDAATNQAVCAIFKNGIFESKFLYYLFFHRKQDLIKEAIGGAQPNISQTILKNLEVTICPLPE
QRAIVSKIEQLFSELENGIANLKLAKEQLKVYRQAVLKKAFEGELTKKWREQQTDLPDAGGLLEQIRKEKEKAAKKAGKK
LKQVKPFTEDELEDLNRLPKEWNWVKIGNLTLGVEYGTSAKSKESGDVAVLRMGNIQNGRFDWSDLVYTSDKTEIEKYLL
SKDDVLFNRTNSPELVGKTAIYKGEKPAIFAGYLIRINQLSELAVADYLNYFLNCHIAKVHGNSVKTDGVNQSNINGEKL
GNYPFPLCSLPEQQTIVQEIETRLSICDKIEQDIETNLEKAEALRQSILKKAFEGKLLNERELAEVRGAEDWEPAEVLLE
RIKAEKAQNGKK
>Mature_412_residues
MLVALYGATIGKLAFLGVDAATNQAVCAIFKNGIFESKFLYYLFFHRKQDLIKEAIGGAQPNISQTILKNLEVTICPLPE
QRAIVSKIEQLFSELENGIANLKLAKEQLKVYRQAVLKKAFEGELTKKWREQQTDLPDAGGLLEQIRKEKEKAAKKAGKK
LKQVKPFTEDELEDLNRLPKEWNWVKIGNLTLGVEYGTSAKSKESGDVAVLRMGNIQNGRFDWSDLVYTSDKTEIEKYLL
SKDDVLFNRTNSPELVGKTAIYKGEKPAIFAGYLIRINQLSELAVADYLNYFLNCHIAKVHGNSVKTDGVNQSNINGEKL
GNYPFPLCSLPEQQTIVQEIETRLSICDKIEQDIETNLEKAEALRQSILKKAFEGKLLNERELAEVRGAEDWEPAEVLLE
RIKAEKAQNGKK

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

Organism=Escherichia coli, GI1790807, Length=357, Percent_Identity=28.0112044817927, Blast_Score=102, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 46440; Mature: 46440

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVALYGATIGKLAFLGVDAATNQAVCAIFKNGIFESKFLYYLFFHRKQDLIKEAIGGAQ
CEEEEECCHHHHHHHEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
PNISQTILKNLEVTICPLPEQRAIVSKIEQLFSELENGIANLKLAKEQLKVYRQAVLKKA
CCHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FEGELTKKWREQQTDLPDAGGLLEQIRKEKEKAAKKAGKKLKQVKPFTEDELEDLNRLPK
HCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCC
EWNWVKIGNLTLGVEYGTSAKSKESGDVAVLRMGNIQNGRFDWSDLVYTSDKTEIEKYLL
CCCEEEECCEEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCHHHCEEECCHHHHHHHHH
SKDDVLFNRTNSPELVGKTAIYKGEKPAIFAGYLIRINQLSELAVADYLNYFLNCHIAKV
CCCCEEEECCCCCHHHCCHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEE
HGNSVKTDGVNQSNINGEKLGNYPFPLCSLPEQQTIVQEIETRLSICDKIEQDIETNLEK
ECCCCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEALRQSILKKAFEGKLLNERELAEVRGAEDWEPAEVLLERIKAEKAQNGKK
HHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLVALYGATIGKLAFLGVDAATNQAVCAIFKNGIFESKFLYYLFFHRKQDLIKEAIGGAQ
CEEEEECCHHHHHHHEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
PNISQTILKNLEVTICPLPEQRAIVSKIEQLFSELENGIANLKLAKEQLKVYRQAVLKKA
CCHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FEGELTKKWREQQTDLPDAGGLLEQIRKEKEKAAKKAGKKLKQVKPFTEDELEDLNRLPK
HCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCC
EWNWVKIGNLTLGVEYGTSAKSKESGDVAVLRMGNIQNGRFDWSDLVYTSDKTEIEKYLL
CCCEEEECCEEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCHHHCEEECCHHHHHHHHH
SKDDVLFNRTNSPELVGKTAIYKGEKPAIFAGYLIRINQLSELAVADYLNYFLNCHIAKV
CCCCEEEECCCCCHHHCCHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEE
HGNSVKTDGVNQSNINGEKLGNYPFPLCSLPEQQTIVQEIETRLSICDKIEQDIETNLEK
ECCCCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEALRQSILKKAFEGKLLNERELAEVRGAEDWEPAEVLLERIKAEKAQNGKK
HHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6304321; 7610040; 9278503; 7739896 [H]