Click here to switch to the map view.

The map label for this gene is mtaC [H]

Identifier: 21227750

GI number: 21227750

Start: 1967461

End: 1968267

Strand: Reverse

Name: mtaC [H]

Synonym: MM_1648

Alternate gene names: 21227750

Gene position: 1968267-1967461 (Counterclockwise)

Preceding gene: 21227754

Following gene: 21227749

Centisome position: 48.05

GC content: 46.96

Gene sequence:

>807_bases
TTGTTAAAATCTAAATGGAGGTTTAGAAATATGTTGGACTTTACAGAGGCAAGTCTGAAAAAGGTATTAACCAGATACAA
TGTAGCTCTGGAAAAGGCAATGACGCCAGAAGACGCCGCCGAAGAGCTCTATCCTAAGGACGAACTTATTTACCCGATTG
CAAAAGCTATCTTCGAAGGAGAAGAAGACGACGTAATCGAGGGTCTCGAAGCAGCAATTAAAGCAGGCAAGGACCCAATT
GCTCTTATCGATGACGCACTAATGGTCGGCATGGGCGTTGTCACAAGACTCTACGATGAAGGTATCATTTTCCTCCCCAA
TGTCATGATGTCCGCTGACGCCATGCTTGATGGTATTGAATTCTGTAAGGAAAACTCCGAAACTGCTCCTGTAACAAAGG
GAACTGTTGTCTGCCACGTCGCAGAAGGTGACGTTCACGACATCGGGAAGAACATCGTTACAGCCCTCCTCAGAGCAAAC
GGCTACAATGTAGTTGACCTCGGAAGGGACGTTCCTGTCGATGAAGTTCTCAATGCAGTTGCTGAAAACAACCCAATCCT
GGTCACAGGTACTGCACTCATGACCACCACCATGTATGCATTCAAGGAAGTTAACGACAAACTTCTCGAGAAAGGATACA
AGATTCCATTCGCATGCGGTGGCGGAGCAGTTAACCAGGACTTCGTATCCCAGTATGCACTCGGTGTATATGGTGAAGAG
GCCGCTGACGCCCCCAAGATTGCTGACGCAATCGTTGCAGGTACCACAGATATTGCAGCATTAAGAGACAAATTCCACAA
GCACTGA

Upstream 100 bases:

>100_bases
AAGAGTATTACCACGGAAAAATAGCTTTACAGAGGCAAAACCGGAACCTCCAGCGCCTTATGAACCCGGAAACTGCAGAT
TCGGAGAGGCAAGGACCTTT

Downstream 100 bases:

>100_bases
GGTGAGTGAAATGGCAGCAACAAGATTCACTAAGATGGCATACGCAAGCGCAGACGAAATGACCTTCGGCGTATCCAAGT
ACCCTGTAAAAGCAGGACTA

Product: methanol corrinoid protein MtaC

Products: L-Methionine; 5,6,7,8-Tetrahydrofolate [C]

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MLKSKWRFRNMLDFTEASLKKVLTRYNVALEKAMTPEDAAEELYPKDELIYPIAKAIFEGEEDDVIEGLEAAIKAGKDPI
ALIDDALMVGMGVVTRLYDEGIIFLPNVMMSADAMLDGIEFCKENSETAPVTKGTVVCHVAEGDVHDIGKNIVTALLRAN
GYNVVDLGRDVPVDEVLNAVAENNPILVTGTALMTTTMYAFKEVNDKLLEKGYKIPFACGGGAVNQDFVSQYALGVYGEE
AADAPKIADAIVAGTTDIAALRDKFHKH

Sequences:

>Translated_268_residues
MLKSKWRFRNMLDFTEASLKKVLTRYNVALEKAMTPEDAAEELYPKDELIYPIAKAIFEGEEDDVIEGLEAAIKAGKDPI
ALIDDALMVGMGVVTRLYDEGIIFLPNVMMSADAMLDGIEFCKENSETAPVTKGTVVCHVAEGDVHDIGKNIVTALLRAN
GYNVVDLGRDVPVDEVLNAVAENNPILVTGTALMTTTMYAFKEVNDKLLEKGYKIPFACGGGAVNQDFVSQYALGVYGEE
AADAPKIADAIVAGTTDIAALRDKFHKH
>Mature_268_residues
MLKSKWRFRNMLDFTEASLKKVLTRYNVALEKAMTPEDAAEELYPKDELIYPIAKAIFEGEEDDVIEGLEAAIKAGKDPI
ALIDDALMVGMGVVTRLYDEGIIFLPNVMMSADAMLDGIEFCKENSETAPVTKGTVVCHVAEGDVHDIGKNIVTALLRAN
GYNVVDLGRDVPVDEVLNAVAENNPILVTGTALMTTTMYAFKEVNDKLLEKGYKIPFACGGGAVNQDFVSQYALGVYGEE
AADAPKIADAIVAGTTDIAALRDKFHKH

Specific function: Acts as a methyl group carrier between mtbB and mtbA [H]

COG id: COG5012

COG function: function code R; Predicted cobalamin binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=188, Percent_Identity=31.3829787234043, Blast_Score=85, Evalue=7e-17,
Organism=Escherichia coli, GI1790450, Length=182, Percent_Identity=35.7142857142857, Blast_Score=105, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17535405, Length=214, Percent_Identity=30.8411214953271, Blast_Score=84, Evalue=7e-17,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR003759
- InterPro:   IPR012741 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2 [H]

EC number: 2.1.1.13 [C]

Molecular weight: Translated: 29109; Mature: 29109

Theoretical pI: Translated: 4.34; Mature: 4.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKSKWRFRNMLDFTEASLKKVLTRYNVALEKAMTPEDAAEELYPKDELIYPIAKAIFEG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHCC
EEDDVIEGLEAAIKAGKDPIALIDDALMVGMGVVTRLYDEGIIFLPNVMMSADAMLDGIE
CCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHHHHHHH
FCKENSETAPVTKGTVVCHVAEGDVHDIGKNIVTALLRANGYNVVDLGRDVPVDEVLNAV
HHHCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHH
AENNPILVTGTALMTTTMYAFKEVNDKLLEKGYKIPFACGGGAVNQDFVSQYALGVYGEE
HCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCHHHHHHHHHHCCCCH
AADAPKIADAIVAGTTDIAALRDKFHKH
HCCCHHHHHHHHHCCHHHHHHHHHHCCC
>Mature Secondary Structure
MLKSKWRFRNMLDFTEASLKKVLTRYNVALEKAMTPEDAAEELYPKDELIYPIAKAIFEG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHCC
EEDDVIEGLEAAIKAGKDPIALIDDALMVGMGVVTRLYDEGIIFLPNVMMSADAMLDGIE
CCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHHHHHHH
FCKENSETAPVTKGTVVCHVAEGDVHDIGKNIVTALLRANGYNVVDLGRDVPVDEVLNAV
HHHCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHH
AENNPILVTGTALMTTTMYAFKEVNDKLLEKGYKIPFACGGGAVNQDFVSQYALGVYGEE
HCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCHHHHHHHHHHCCCCH
AADAPKIADAIVAGTTDIAALRDKFHKH
HCCCHHHHHHHHHCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: ATP; Cobinamide; S-adenosyl-L-methionine [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 500-780 [C]

Specific activity: 3.8

Km value (mM): 0.035 {S-methyltetrahydrofolate}} 0.0016 {S-adenosyl-L-methionine}} 0.00065 {S-adenosyl-L-methionine}} 0.06 {methyltetrahydrofolate}} 0.03 {methyltetrahydrofolate}} 0.016 {homocysteine}} [C]

Substrates: 5-Methyltetrahydrofolate; L-Homocysteine [C]

Specific reaction: 5-Methyltetrahydrofolate + L-Homocysteine --> L-Methionine + 5,6,7,8-Tetrahydrofolate [C]

General reaction: Methyl group transfer [C]

Inhibitor: 5, 6-Dimethyl benzimidazole; alpha-Ribazole 3'-phosphate; Be2+; Cobinamide; Propyl iodide; Sr2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12125824 [H]