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The map label for this gene is truA

Identifier: 21227597

GI number: 21227597

Start: 1782836

End: 1783645

Strand: Direct

Name: truA

Synonym: MM_1495

Alternate gene names: 21227597

Gene position: 1782836-1783645 (Clockwise)

Preceding gene: 21227596

Following gene: 21227605

Centisome position: 43.52

GC content: 44.94

Gene sequence:

>810_bases
ATGAGAGTCGCTTTAAAACTTGCATACATAGGCACCGAGTTTCATGGATCTCAGATTCAGCCTAGTGTTGAAACAGTGGA
GAAAGAACTCTTCAAAGCTCTTCGGAACCTCAGGATTATAGAAAGCCCCAAATCTGCTAACTATATCTGCGCGGGCAGGA
CAGATGCGGGTGTTCATGCTCTTGGACAGGTTATAGCTTTTGATACGGACAGGCCAAACCTGGCAATTCCCAGAATCATA
AACTCCGGACTTCCTCCAACCATATGGGTATGGGCTCATGCAGAAGTCCCCTACGATTTTGATGCCAGGAGGCATGCAGT
TTCCAGGCATTATAGATACGTAATTAGCGGTGAAGGGTACGATATCTCAAAAATGAGGGAAGCTTCAAAACTGCTTCTCG
GAACCCATGATTTCGAAAACTTTTCCAGATCAAACGGAGAGAAAAGTACGGTTCGTACCCTGGAAAGGATCAACGTCCGC
GTGGATGGAGATATTACAAAGATTGACGTTGTAGGCAACAGTTTTCTCTGGAATATGGTGAGGAAAATAGTAACCGCCCT
TTCAATGATCGGAAAAGGTGTACGTGACAATGACTGGCTGATCCAGATGTTAAATCCTGATATTTATGAAGAAGGTATTG
AACCGGCTCCTGCCTATGGGTTGACCCTTATGGGAGTAAACTACGGAGAGGAAATAAACTGGATTGAAGATAACTACTCA
ATCCGAAGGGCAAGTGACCAGAACTCCAAGCGCATCCTCAGGTACAGGGTTATGGCTGAAGTGCTCGAGGAATTGATTTA
CCATGAGTAA

Upstream 100 bases:

>100_bases
ATCCGGGGATCTGGTTCCTTTTCGGGTGCATCTTCGTGATATTTCTGGTAGTTGTAAGAGAAATCTATTATAAGAAAAAA
ACCAAGTAATCAGGGATTGT

Downstream 100 bases:

>100_bases
ATAACTTCATTTCCGTCTTCACTACTTTTTTTGCTTTTCCTATCAATTTTTTATCTTTATTCAGGTTCTGGCTCAGTCTC
TGGCTCAGTTTCTCACTTTT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MRVALKLAYIGTEFHGSQIQPSVETVEKELFKALRNLRIIESPKSANYICAGRTDAGVHALGQVIAFDTDRPNLAIPRII
NSGLPPTIWVWAHAEVPYDFDARRHAVSRHYRYVISGEGYDISKMREASKLLLGTHDFENFSRSNGEKSTVRTLERINVR
VDGDITKIDVVGNSFLWNMVRKIVTALSMIGKGVRDNDWLIQMLNPDIYEEGIEPAPAYGLTLMGVNYGEEINWIEDNYS
IRRASDQNSKRILRYRVMAEVLEELIYHE

Sequences:

>Translated_269_residues
MRVALKLAYIGTEFHGSQIQPSVETVEKELFKALRNLRIIESPKSANYICAGRTDAGVHALGQVIAFDTDRPNLAIPRII
NSGLPPTIWVWAHAEVPYDFDARRHAVSRHYRYVISGEGYDISKMREASKLLLGTHDFENFSRSNGEKSTVRTLERINVR
VDGDITKIDVVGNSFLWNMVRKIVTALSMIGKGVRDNDWLIQMLNPDIYEEGIEPAPAYGLTLMGVNYGEEINWIEDNYS
IRRASDQNSKRILRYRVMAEVLEELIYHE
>Mature_269_residues
MRVALKLAYIGTEFHGSQIQPSVETVEKELFKALRNLRIIESPKSANYICAGRTDAGVHALGQVIAFDTDRPNLAIPRII
NSGLPPTIWVWAHAEVPYDFDARRHAVSRHYRYVISGEGYDISKMREASKLLLGTHDFENFSRSNGEKSTVRTLERINVR
VDGDITKIDVVGNSFLWNMVRKIVTALSMIGKGVRDNDWLIQMLNPDIYEEGIEPAPAYGLTLMGVNYGEEINWIEDNYS
IRRASDQNSKRILRYRVMAEVLEELIYHE

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI199559785, Length=277, Percent_Identity=30.6859205776173, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI23503259, Length=269, Percent_Identity=28.2527881040892, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1788657, Length=246, Percent_Identity=33.739837398374, Blast_Score=119, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI71985046, Length=308, Percent_Identity=25.6493506493506, Blast_Score=104, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6321112, Length=293, Percent_Identity=27.9863481228669, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6325044, Length=119, Percent_Identity=36.1344537815126, Blast_Score=70, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6321375, Length=114, Percent_Identity=32.4561403508772, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19922728, Length=252, Percent_Identity=31.3492063492063, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI281362146, Length=259, Percent_Identity=26.2548262548263, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24648455, Length=259, Percent_Identity=26.2548262548263, Blast_Score=72, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_METMA (Q8PWT1)

Other databases:

- EMBL:   AE008384
- RefSeq:   NP_633519.1
- ProteinModelPortal:   Q8PWT1
- GeneID:   1479837
- GenomeReviews:   AE008384_GR
- KEGG:   mma:MM_1495
- NMPDR:   fig|192952.1.peg.1495
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- ProtClustDB:   PRK00021
- BioCyc:   MMAZ192952:MM1495-MONOMER
- BRENDA:   5.4.99.12
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 30684; Mature: 30684

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: ACT_SITE 55-55

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVALKLAYIGTEFHGSQIQPSVETVEKELFKALRNLRIIESPKSANYICAGRTDAGVHA
CCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCEEEECCCCHHHHH
LGQVIAFDTDRPNLAIPRIINSGLPPTIWVWAHAEVPYDFDARRHAVSRHYRYVISGEGY
HHHHEEECCCCCCEEHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHEEEEECCCCC
DISKMREASKLLLGTHDFENFSRSNGEKSTVRTLERINVRVDGDITKIDVVGNSFLWNMV
CHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHEEEECCCEEEEEEECCHHHHHHH
RKIVTALSMIGKGVRDNDWLIQMLNPDIYEEGIEPAPAYGLTLMGVNYGEEINWIEDNYS
HHHHHHHHHHCCCCCCCCCEEEECCCHHHHHCCCCCCCCCEEEEECCCCCCCCEECCCCE
IRRASDQNSKRILRYRVMAEVLEELIYHE
EEECCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRVALKLAYIGTEFHGSQIQPSVETVEKELFKALRNLRIIESPKSANYICAGRTDAGVHA
CCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCEEEECCCCHHHHH
LGQVIAFDTDRPNLAIPRIINSGLPPTIWVWAHAEVPYDFDARRHAVSRHYRYVISGEGY
HHHHEEECCCCCCEEHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHEEEEECCCCC
DISKMREASKLLLGTHDFENFSRSNGEKSTVRTLERINVRVDGDITKIDVVGNSFLWNMV
CHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHEEEECCCEEEEEEECCHHHHHHH
RKIVTALSMIGKGVRDNDWLIQMLNPDIYEEGIEPAPAYGLTLMGVNYGEEINWIEDNYS
HHHHHHHHHHCCCCCCCCCEEEECCCHHHHHCCCCCCCCCEEEEECCCCCCCCEECCCCE
IRRASDQNSKRILRYRVMAEVLEELIYHE
EEECCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12125824