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The map label for this gene is leuD [H]

Identifier: 21227592

GI number: 21227592

Start: 1776555

End: 1777043

Strand: Reverse

Name: leuD [H]

Synonym: MM_1490

Alternate gene names: 21227592

Gene position: 1777043-1776555 (Counterclockwise)

Preceding gene: 21227593

Following gene: 21227591

Centisome position: 43.38

GC content: 47.65

Gene sequence:

>489_bases
ATGAAAGGAAGAGCCTGGAAATTCGGAGATGACGTGGATACGGATGCAGTGATACCCGGAAGGTACCTGATATTCAATAC
CCCGGGAGAGCTTGCTAAGTATACATTTGAAGGTGTGCGCCCTGATTTCGCAAAAAATGTTCATGAAAATGACATTGTGG
TTGCAGGAAGTAATTTCGGCTGCGGGTCCTCGCGCGAACATGCACCCCTCGCCCTTAAAGGGTCAAAGGTATCCTGTGTG
ATTGCAAAGTCTTTCGCAAGGATCTTTTTCAGAAACGCGATCAATATCGGAGTTCCTGTCCTTGAATGCCCGGATACGGA
TAAAATAGATGACGGAGATGAACTCGAAGTAGATATTTCAACAGGGGTCATTCAGAACAGGACAAAAGGAGAGACCTATC
AGGCAACCCCTCTACCGGATTTCGTTCGCGAAATCGTGGATGAGGGAGGACTTATAGAATATGCCCGGAAGCTGGTCTCT
GAGCGCTGA

Upstream 100 bases:

>100_bases
ATTACCTGTGATCTTCAAACCTGTGAGTTTAGCTGAGTTACAGGCTGTGAATATTATATCAATCAACAGCATTTAATTAC
AGCAAATTACTGGTGATTTC

Downstream 100 bases:

>100_bases
AAAACAGGAAGCCTGTCCCGAAAAAATCCTGATCCACGTTTGACATCTCGCACTGAAAAGGCAGTTTTGCCTCTAAACTA
GAAAGGAGTATAAACATGAC

Product: 3-isopropylmalate dehydratase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 2; IPMI 2; Isopropylmalate isomerase 2 [H]

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MKGRAWKFGDDVDTDAVIPGRYLIFNTPGELAKYTFEGVRPDFAKNVHENDIVVAGSNFGCGSSREHAPLALKGSKVSCV
IAKSFARIFFRNAINIGVPVLECPDTDKIDDGDELEVDISTGVIQNRTKGETYQATPLPDFVREIVDEGGLIEYARKLVS
ER

Sequences:

>Translated_162_residues
MKGRAWKFGDDVDTDAVIPGRYLIFNTPGELAKYTFEGVRPDFAKNVHENDIVVAGSNFGCGSSREHAPLALKGSKVSCV
IAKSFARIFFRNAINIGVPVLECPDTDKIDDGDELEVDISTGVIQNRTKGETYQATPLPDFVREIVDEGGLIEYARKLVS
ER
>Mature_162_residues
MKGRAWKFGDDVDTDAVIPGRYLIFNTPGELAKYTFEGVRPDFAKNVHENDIVVAGSNFGCGSSREHAPLALKGSKVSCV
IAKSFARIFFRNAINIGVPVLECPDTDKIDDGDELEVDISTGVIQNRTKGETYQATPLPDFVREIVDEGGLIEYARKLVS
ER

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=147, Percent_Identity=34.0136054421769, Blast_Score=67, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6320440, Length=98, Percent_Identity=41.8367346938776, Blast_Score=76, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6321429, Length=103, Percent_Identity=41.747572815534, Blast_Score=64, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR011827 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 17810; Mature: 17810

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGRAWKFGDDVDTDAVIPGRYLIFNTPGELAKYTFEGVRPDFAKNVHENDIVVAGSNFG
CCCCCCCCCCCCCCCEECCCCEEEECCCHHHHHHHHCCCCCHHHCCCCCCCEEEECCCCC
CGSSREHAPLALKGSKVSCVIAKSFARIFFRNAINIGVPVLECPDTDKIDDGDELEVDIS
CCCCCCCCCEEEECCCEEEHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEEEEC
TGVIQNRTKGETYQATPLPDFVREIVDEGGLIEYARKLVSER
CCEECCCCCCCCEECCCCHHHHHHHHCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MKGRAWKFGDDVDTDAVIPGRYLIFNTPGELAKYTFEGVRPDFAKNVHENDIVVAGSNFG
CCCCCCCCCCCCCCCEECCCCEEEECCCHHHHHHHHCCCCCHHHCCCCCCCEEEECCCCC
CGSSREHAPLALKGSKVSCVIAKSFARIFFRNAINIGVPVLECPDTDKIDDGDELEVDIS
CCCCCCCCCEEEECCCEEEHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEEEEC
TGVIQNRTKGETYQATPLPDFVREIVDEGGLIEYARKLVSER
CCEECCCCCCCCEECCCCHHHHHHHHCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]