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The map label for this gene is leuB [H]

Identifier: 21227591

GI number: 21227591

Start: 1775332

End: 1776459

Strand: Reverse

Name: leuB [H]

Synonym: MM_1489

Alternate gene names: 21227591

Gene position: 1776459-1775332 (Counterclockwise)

Preceding gene: 21227592

Following gene: 21227590

Centisome position: 43.37

GC content: 48.58

Gene sequence:

>1128_bases
ATGACGCAGTATAAGGTTCCCGTCCTCCCAGGAGATGGGATTGGACCAGAAATCATCGCCGAAGGCAGAAAAGTCATAGA
TGCTGCAGGCGAAAGATTTGGTTTTGATGTAGAATGGATAGAATATCCTCATGGAGCAGACCACTACCTGGAAACGGGTG
AGCTGATCTCGGAAGAGACCTTAAAGGAATTATCCGGCTACCCTGCAATTTATCTTGGCTCCATAGGAGACCCGAGGATT
GCCCCAGGTGTCCTTGAAAAGGGGATCTTATTAACTGCACGCTTTTACTTTGACCAGTACATTAACCTGCGCCCGATAAA
ACTCCTTGAGGGTGTCTGGACCCCGATAAAGGACAAGACATCAAAAGACATTGACTTTGTCGTGGTAAGGGAAAATACCG
AGGACTTCTACATAGGCATTGGCGGCAGGGCAAAAAAAGGAGCGAGTAAAGACCTCCTTGAAGTCAAGAGGACACTTTAC
TCCGCAAAGTTCGGCCTCGACATCGAGACCGACAGCGAAGAAATAGGATACCAGATAGGCCTTATCTCCAAAGAGGGCAC
AAAGCGTGTTATTGAGTACGCTTTTGACCTTGCAGAAAACCGGAAGAAACACGTCTCATCTGTTGACAAGGCAAACGTCC
TTTCCGACATCTACGGGTTCTGGAGAGAAGAGTTCAACGCAGTTGCCGAAAAGCATCCTGGTTTTACCACAGACTTCAAC
TTCGTTGACGCTATCACAATGTGGTTTGTGAAAAACCCTGAATGGTTCGATGTGGTAGTAACCCCGAACATGTTCGGAGA
CATCATCACCGACCTCGGAGCCATGATCCAGGGCGGCCTCGGACTCGCCCCGGGCGGGAACATTAACCCGAACGGCACAA
GCATGTTTGAGCCCATCCACGGTTCAGCCCCTAAGTACAAGGGCCAGAACAAGGTAAACCCGATCGCAACAATCTGGGCA
GGTGCAATGCTCATAGAACAGCTCGGAGAAAAGGAAGCCGCAGATACCATAGTCAATGCAATCCAGAAAAATATCCTGGA
CGGCAAAGTAAAGACCTACGATATGGGCGGCAAAAATACTACCTCAGATGTCGGAGACGACATTGCAAGGATAATAAAGG
GAGAATAA

Upstream 100 bases:

>100_bases
GCTGAAAAACAGGAAGCCTGTCCCGAAAAAATCCTGATCCACGTTTGACATCTCGCACTGAAAAGGCAGTTTTGCCTCTA
AACTAGAAAGGAGTATAAAC

Downstream 100 bases:

>100_bases
TTTAAACCTCCCTCCACTACATTTTCTTTTCTGAATAAAGTCAAGTTTCAAGGACAATTTTGAAGTAAACTCCAAAAATC
TCCGGGCTATTATTACAATT

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MTQYKVPVLPGDGIGPEIIAEGRKVIDAAGERFGFDVEWIEYPHGADHYLETGELISEETLKELSGYPAIYLGSIGDPRI
APGVLEKGILLTARFYFDQYINLRPIKLLEGVWTPIKDKTSKDIDFVVVRENTEDFYIGIGGRAKKGASKDLLEVKRTLY
SAKFGLDIETDSEEIGYQIGLISKEGTKRVIEYAFDLAENRKKHVSSVDKANVLSDIYGFWREEFNAVAEKHPGFTTDFN
FVDAITMWFVKNPEWFDVVVTPNMFGDIITDLGAMIQGGLGLAPGGNINPNGTSMFEPIHGSAPKYKGQNKVNPIATIWA
GAMLIEQLGEKEAADTIVNAIQKNILDGKVKTYDMGGKNTTSDVGDDIARIIKGE

Sequences:

>Translated_375_residues
MTQYKVPVLPGDGIGPEIIAEGRKVIDAAGERFGFDVEWIEYPHGADHYLETGELISEETLKELSGYPAIYLGSIGDPRI
APGVLEKGILLTARFYFDQYINLRPIKLLEGVWTPIKDKTSKDIDFVVVRENTEDFYIGIGGRAKKGASKDLLEVKRTLY
SAKFGLDIETDSEEIGYQIGLISKEGTKRVIEYAFDLAENRKKHVSSVDKANVLSDIYGFWREEFNAVAEKHPGFTTDFN
FVDAITMWFVKNPEWFDVVVTPNMFGDIITDLGAMIQGGLGLAPGGNINPNGTSMFEPIHGSAPKYKGQNKVNPIATIWA
GAMLIEQLGEKEAADTIVNAIQKNILDGKVKTYDMGGKNTTSDVGDDIARIIKGE
>Mature_374_residues
TQYKVPVLPGDGIGPEIIAEGRKVIDAAGERFGFDVEWIEYPHGADHYLETGELISEETLKELSGYPAIYLGSIGDPRIA
PGVLEKGILLTARFYFDQYINLRPIKLLEGVWTPIKDKTSKDIDFVVVRENTEDFYIGIGGRAKKGASKDLLEVKRTLYS
AKFGLDIETDSEEIGYQIGLISKEGTKRVIEYAFDLAENRKKHVSSVDKANVLSDIYGFWREEFNAVAEKHPGFTTDFNF
VDAITMWFVKNPEWFDVVVTPNMFGDIITDLGAMIQGGLGLAPGGNINPNGTSMFEPIHGSAPKYKGQNKVNPIATIWAG
AMLIEQLGEKEAADTIVNAIQKNILDGKVKTYDMGGKNTTSDVGDDIARIIKGE

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=369, Percent_Identity=32.7913279132791, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI4758582, Length=373, Percent_Identity=28.686327077748, Blast_Score=135, Evalue=5e-32,
Organism=Homo sapiens, GI28178821, Length=366, Percent_Identity=28.1420765027322, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI28178816, Length=371, Percent_Identity=27.4932614555256, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI28178838, Length=351, Percent_Identity=27.9202279202279, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI28178819, Length=186, Percent_Identity=30.6451612903226, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1788101, Length=386, Percent_Identity=39.6373056994819, Blast_Score=265, Evalue=3e-72,
Organism=Escherichia coli, GI87081683, Length=380, Percent_Identity=32.6315789473684, Blast_Score=191, Evalue=7e-50,
Organism=Escherichia coli, GI1787381, Length=378, Percent_Identity=31.7460317460317, Blast_Score=150, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI71986051, Length=370, Percent_Identity=30.2702702702703, Blast_Score=165, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17550882, Length=373, Percent_Identity=28.1501340482574, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17505779, Length=364, Percent_Identity=27.7472527472527, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI25144293, Length=375, Percent_Identity=24, Blast_Score=114, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6322097, Length=371, Percent_Identity=32.0754716981132, Blast_Score=183, Evalue=4e-47,
Organism=Saccharomyces cerevisiae, GI6319830, Length=389, Percent_Identity=32.3907455012853, Blast_Score=157, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6324709, Length=373, Percent_Identity=29.2225201072386, Blast_Score=144, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6324291, Length=376, Percent_Identity=30.5851063829787, Blast_Score=133, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24643270, Length=377, Percent_Identity=31.8302387267904, Blast_Score=171, Evalue=8e-43,
Organism=Drosophila melanogaster, GI24643268, Length=377, Percent_Identity=31.8302387267904, Blast_Score=171, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24661184, Length=377, Percent_Identity=30.5039787798408, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI161078637, Length=375, Percent_Identity=25.6, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI161078635, Length=375, Percent_Identity=25.6, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI161078633, Length=375, Percent_Identity=25.6, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24650122, Length=375, Percent_Identity=25.6, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI161078639, Length=370, Percent_Identity=25.4054054054054, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI281362242, Length=364, Percent_Identity=26.6483516483516, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24648872, Length=364, Percent_Identity=26.6483516483516, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI20130355, Length=362, Percent_Identity=23.2044198895028, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 41396; Mature: 41265

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQYKVPVLPGDGIGPEIIAEGRKVIDAAGERFGFDVEWIEYPHGADHYLETGELISEET
CCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHH
LKELSGYPAIYLGSIGDPRIAPGVLEKGILLTARFYFDQYINLRPIKLLEGVWTPIKDKT
HHHHCCCCEEEECCCCCCCCCCHHHHCCHHEEHHHHHHHHCCCCHHHHHHHCCCCCCCCC
SKDIDFVVVRENTEDFYIGIGGRAKKGASKDLLEVKRTLYSAKFGLDIETDSEEIGYQIG
CCCCEEEEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCEECCCHHHHCEEEE
LISKEGTKRVIEYAFDLAENRKKHVSSVDKANVLSDIYGFWREEFNAVAEKHPGFTTDFN
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
FVDAITMWFVKNPEWFDVVVTPNMFGDIITDLGAMIQGGLGLAPGGNINPNGTSMFEPIH
HHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
GSAPKYKGQNKVNPIATIWAGAMLIEQLGEKEAADTIVNAIQKNILDGKVKTYDMGGKNT
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCC
TSDVGDDIARIIKGE
CHHHHHHHHHHHCCC
>Mature Secondary Structure 
TQYKVPVLPGDGIGPEIIAEGRKVIDAAGERFGFDVEWIEYPHGADHYLETGELISEET
CCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHH
LKELSGYPAIYLGSIGDPRIAPGVLEKGILLTARFYFDQYINLRPIKLLEGVWTPIKDKT
HHHHCCCCEEEECCCCCCCCCCHHHHCCHHEEHHHHHHHHCCCCHHHHHHHCCCCCCCCC
SKDIDFVVVRENTEDFYIGIGGRAKKGASKDLLEVKRTLYSAKFGLDIETDSEEIGYQIG
CCCCEEEEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCEECCCHHHHCEEEE
LISKEGTKRVIEYAFDLAENRKKHVSSVDKANVLSDIYGFWREEFNAVAEKHPGFTTDFN
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
FVDAITMWFVKNPEWFDVVVTPNMFGDIITDLGAMIQGGLGLAPGGNINPNGTSMFEPIH
HHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
GSAPKYKGQNKVNPIATIWAGAMLIEQLGEKEAADTIVNAIQKNILDGKVKTYDMGGKNT
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCC
TSDVGDDIARIIKGE
CHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA