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The map label for this gene is cofE

Identifier: 21227523

GI number: 21227523

Start: 1703918

End: 1704682

Strand: Direct

Name: cofE

Synonym: MM_1421

Alternate gene names: 21227523

Gene position: 1703918-1704682 (Clockwise)

Preceding gene: 21227521

Following gene: 21227526

Centisome position: 41.6

GC content: 44.18

Gene sequence:

>765_bases
TTGAAATTCGAAGCAATTGCCGTAGAAAAAATCCCTCTCATACATGAAGGAGACAACCTGCCCTCCATCATCTGTGAGAA
AATTGGACTTCAGGACAGAGACATTATGATTATTGCATCAACCATTGTTGCAAAAGCCGAAGGGGAAATTTTCAGGCTCG
AAGATATCACCCCTGGAGAAGAAGCTCTTGAAATTGCAGCCCGGACAGGAAAAGATCCTCGTTTTATACAGGCGGTCCTT
TCAAAGAGCAGGGAAGTCTTTGTCGAGACACCTTTTATGCTTGTGACGACTCTCGCAGGGCACACCTGTGTTAATGCAGG
AATTGACGAGTCTAATATTGAGAACGGGTTTTTACTGTACCCCCCTGAAAACCCGGATGCAAGTGCCTTAAAAATCGGAA
AGGAACTTGAAAAGCTTAGCGGGAAAAAGTTAAGCATTATTATTACGGACACAAACGGAAGAGCTTTCAAGATCGGGCAG
ACTGGAGTTGCCATCGGAATTTATAATATAAAACCTGTAAAACGCTGGATAGGGGAAAAGGACCTTTTCGGCAAAGTCCT
GGAAATCACAGAGCAGGCAATTGCTGACGAACTTGCAGGTGCTGCCAATCTTTTAATGGGAGAAGGAGCAGGTGGAATCC
CGGTTGTAATCATCCGCGGGCTTGATTACTACTGTGAAGGGGATACATCTATAAAAGAGTCATACCGCCCTGAAAACATG
GATGTCATTAAACAGGGTCTTCGGTGCTTGAGAAATAAGAAATGA

Upstream 100 bases:

>100_bases
ACTGCCTTTACTGGTCTAAATTATTCATTTATGGGTTTTTGTAACTCTGTAAACCAGCCCGCTCAATATTCTATCATTTT
AAATTATTTGAGGGAATCCT

Downstream 100 bases:

>100_bases
GCTGGAAAAAATGTTAAAAACATTATTGGATTAATGTTAAAAACATATTGGATTAATGTTAAAAACATATTGGATTAATG
TTAAAAACATATTGGATTAA

Product: F420-0--gamma-glutamyl ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MKFEAIAVEKIPLIHEGDNLPSIICEKIGLQDRDIMIIASTIVAKAEGEIFRLEDITPGEEALEIAARTGKDPRFIQAVL
SKSREVFVETPFMLVTTLAGHTCVNAGIDESNIENGFLLYPPENPDASALKIGKELEKLSGKKLSIIITDTNGRAFKIGQ
TGVAIGIYNIKPVKRWIGEKDLFGKVLEITEQAIADELAGAANLLMGEGAGGIPVVIIRGLDYYCEGDTSIKESYRPENM
DVIKQGLRCLRNKK

Sequences:

>Translated_254_residues
MKFEAIAVEKIPLIHEGDNLPSIICEKIGLQDRDIMIIASTIVAKAEGEIFRLEDITPGEEALEIAARTGKDPRFIQAVL
SKSREVFVETPFMLVTTLAGHTCVNAGIDESNIENGFLLYPPENPDASALKIGKELEKLSGKKLSIIITDTNGRAFKIGQ
TGVAIGIYNIKPVKRWIGEKDLFGKVLEITEQAIADELAGAANLLMGEGAGGIPVVIIRGLDYYCEGDTSIKESYRPENM
DVIKQGLRCLRNKK
>Mature_254_residues
MKFEAIAVEKIPLIHEGDNLPSIICEKIGLQDRDIMIIASTIVAKAEGEIFRLEDITPGEEALEIAARTGKDPRFIQAVL
SKSREVFVETPFMLVTTLAGHTCVNAGIDESNIENGFLLYPPENPDASALKIGKELEKLSGKKLSIIITDTNGRAFKIGQ
TGVAIGIYNIKPVKRWIGEKDLFGKVLEITEQAIADELAGAANLLMGEGAGGIPVVIIRGLDYYCEGDTSIKESYRPENM
DVIKQGLRCLRNKK

Specific function: Catalyzes the GTP-dependent successive addition of multiple gamma-linked L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form polyglutamated F420 derivatives

COG id: COG1478

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CofE family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COFE_METMA (Q8PX01)

Other databases:

- EMBL:   AE008384
- RefSeq:   NP_633445.1
- ProteinModelPortal:   Q8PX01
- SMR:   Q8PX01
- GeneID:   1479763
- GenomeReviews:   AE008384_GR
- KEGG:   mma:MM_1421
- NMPDR:   fig|192952.1.peg.1421
- HOGENOM:   HBG578188
- OMA:   GEKDLFG
- ProtClustDB:   PRK13293
- BioCyc:   MMAZ192952:MM1421-MONOMER
- HAMAP:   MF_01258
- InterPro:   IPR002847
- InterPro:   IPR008225
- TIGRFAMs:   TIGR01916

Pfam domain/function: PF01996 F420_ligase

EC number: NA

Molecular weight: Translated: 27742; Mature: 27742

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFEAIAVEKIPLIHEGDNLPSIICEKIGLQDRDIMIIASTIVAKAEGEIFRLEDITPGE
CCCCEEEEECCCEEECCCCCHHHHHHHCCCCCCCEEEEEEEHHHHCCCCEEEEECCCCCH
EALEIAARTGKDPRFIQAVLSKSREVFVETPFMLVTTLAGHTCVNAGIDESNIENGFLLY
HHHHHHHHCCCCHHHHHHHHHCCCEEEEECCHHHHHHHHCCHHEECCCCCCCCCCCEEEE
PPENPDASALKIGKELEKLSGKKLSIIITDTNGRAFKIGQTGVAIGIYNIKPVKRWIGEK
CCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEECCCCEEEEEECCHHHHHHCCCH
DLFGKVLEITEQAIADELAGAANLLMGEGAGGIPVVIIRGLDYYCEGDTSIKESYRPENM
HHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEEECCCEEECCCCCHHHCCCCCCH
DVIKQGLRCLRNKK
HHHHHHHHHHHCCC
>Mature Secondary Structure
MKFEAIAVEKIPLIHEGDNLPSIICEKIGLQDRDIMIIASTIVAKAEGEIFRLEDITPGE
CCCCEEEEECCCEEECCCCCHHHHHHHCCCCCCCEEEEEEEHHHHCCCCEEEEECCCCCH
EALEIAARTGKDPRFIQAVLSKSREVFVETPFMLVTTLAGHTCVNAGIDESNIENGFLLY
HHHHHHHHCCCCHHHHHHHHHCCCEEEEECCHHHHHHHHCCHHEECCCCCCCCCCCEEEE
PPENPDASALKIGKELEKLSGKKLSIIITDTNGRAFKIGQTGVAIGIYNIKPVKRWIGEK
CCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEECCCCEEEEEECCHHHHHHCCCH
DLFGKVLEITEQAIADELAGAANLLMGEGAGGIPVVIIRGLDYYCEGDTSIKESYRPENM
HHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEEECCCEEECCCCCHHHCCCCCCH
DVIKQGLRCLRNKK
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12125824