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The map label for this gene is thrA [C]

Identifier: 21227519

GI number: 21227519

Start: 1699001

End: 1700440

Strand: Reverse

Name: thrA [C]

Synonym: MM_1417

Alternate gene names: 21227519

Gene position: 1700440-1699001 (Counterclockwise)

Preceding gene: 21227522

Following gene: 21227518

Centisome position: 41.51

GC content: 47.08

Gene sequence:

>1440_bases
ATGCTAAAACGTGATCCGATCATGAAAATAGTAATGAAATTTGGAGGAACTTCCGTTGGGGACGGAAAAAAGATCCGTCA
TGTTGCCCATCTTCTCAAAAAATACAATGAGGAGGGCAACCAGATTGTGGTGGTAACCTCGGCACTCGGTGGAGTAACCG
ATAGGCTTCTGGAAAATGCTCGCCTTGCCTCAACAAAGGGCAAGGTTTCGCTTGTCAAAGAATTTAAAACAGAACTTACA
AACAAACACCACGAAGCCGTGAGGGACGCCATTGAAGACCCCAATGTCGCAAAAGATGTCCTCCACATCCTTGACCTCAG
GATTGACGAGCTTGAAAAAGCCCTGATCGGTATCTGCTATCTGGGTGAACTTACAAACAGGTCAATCGATTATATTTCTT
CGTACGGAGAACGTCTGGCGGCTCCAATAATTTCAGGCGCTGTCCGATCCCTGGGGATCCCATCAATCGAATACACCGGG
GGAGAGGCAGGGATTTTAACCACATCGGATTACGGAAACGCCCGTCCTCTTGAAAAGACATACGAGCTCGTATCAAAAAG
GCTTGGGTGCAGGCTTGATTCTCATATCCTTGTGGTCACAGGCTTCATAGGGGAAAATGAAGACGGGATAATTACCACCC
TTGGAAGAAGCGGGTCCGATTTCTCAGCTTCCATACTTGGAGCAGCCCTGAAAGCTGACGAGATCTGGCTCTGGAAAGAA
GTTAACGGGATCATGACAACGGACCCGAGAATCGTTCCCGAAGCAAAGACCATCCCGCAGATCTCATATGCAGAAGCCAT
GGAACTCTCTTATTTCGGAGCAAACGTTCTGCACCCGCGTACTATTGAACCGGCAATGCGCGAACACATTCCCGTAAGGG
TCAAAAACACCTTTGATCCGGAGTTCCCCGGTACACTGGTAGTATCTGAGAAGTTCCAGTGCAAGCATGTTGTAAAGGCA
GTGAGCCTGATCAAAAATGTAGCCCTTATTAACATCTCAGGCGCGGAAATGGCAGGGACAATCGGGACTGTAGCAAGGCT
CTTTACCGCTCTTGCAAGGGCAGGGGTAAATATTGTTATGATCAGCCAGGGGTCCTCAGAGTCCAACCTATCTTTCGTTG
TCAGTGAGGCTCATGTGGAACCTGCGTTAAAAGCTCTTCATGCAGAATTCAACCGTGAAATCATAAAGGAAATTACTTCG
GACAAAAATGTATGTGTGGTTGCGGTTGTTGGTGCTGGGATGGCAGGAACTCCCGGAGTGGCAAAAAGGGTATTCGGAGC
ACTTGGGAATTCAATGATTAATATTATTATGATAAGCCAGGGCTCTTCCCAGTACAATATCTCTTTCGTTGTCAGGGAAG
ATGACGCATTTGCCGCAGTCAAGACACTGCACGACGAATTCGAACTTTATAATGGAAATGGAATTGAAAAAAAGCTGTAA

Upstream 100 bases:

>100_bases
ATTTAGCCGTAAATTCTGATCGCAATGGTCAGATTAATTAAGATTCAGAACACTAAGGATAACATAGTACAAAAAATTAA
AATGTGAGATACCCGACTTA

Downstream 100 bases:

>100_bases
AGCTTGCAGGAATGGTCACAGAATGAGTGAAAAGCACTTAACATACGCGGATTCGGGCGTGGATATTAAAGAGGAAGAAA
AAACTGTCAAAACCCTTATC

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MLKRDPIMKIVMKFGGTSVGDGKKIRHVAHLLKKYNEEGNQIVVVTSALGGVTDRLLENARLASTKGKVSLVKEFKTELT
NKHHEAVRDAIEDPNVAKDVLHILDLRIDELEKALIGICYLGELTNRSIDYISSYGERLAAPIISGAVRSLGIPSIEYTG
GEAGILTTSDYGNARPLEKTYELVSKRLGCRLDSHILVVTGFIGENEDGIITTLGRSGSDFSASILGAALKADEIWLWKE
VNGIMTTDPRIVPEAKTIPQISYAEAMELSYFGANVLHPRTIEPAMREHIPVRVKNTFDPEFPGTLVVSEKFQCKHVVKA
VSLIKNVALINISGAEMAGTIGTVARLFTALARAGVNIVMISQGSSESNLSFVVSEAHVEPALKALHAEFNREIIKEITS
DKNVCVVAVVGAGMAGTPGVAKRVFGALGNSMINIIMISQGSSQYNISFVVREDDAFAAVKTLHDEFELYNGNGIEKKL

Sequences:

>Translated_479_residues
MLKRDPIMKIVMKFGGTSVGDGKKIRHVAHLLKKYNEEGNQIVVVTSALGGVTDRLLENARLASTKGKVSLVKEFKTELT
NKHHEAVRDAIEDPNVAKDVLHILDLRIDELEKALIGICYLGELTNRSIDYISSYGERLAAPIISGAVRSLGIPSIEYTG
GEAGILTTSDYGNARPLEKTYELVSKRLGCRLDSHILVVTGFIGENEDGIITTLGRSGSDFSASILGAALKADEIWLWKE
VNGIMTTDPRIVPEAKTIPQISYAEAMELSYFGANVLHPRTIEPAMREHIPVRVKNTFDPEFPGTLVVSEKFQCKHVVKA
VSLIKNVALINISGAEMAGTIGTVARLFTALARAGVNIVMISQGSSESNLSFVVSEAHVEPALKALHAEFNREIIKEITS
DKNVCVVAVVGAGMAGTPGVAKRVFGALGNSMINIIMISQGSSQYNISFVVREDDAFAAVKTLHDEFELYNGNGIEKKL
>Mature_479_residues
MLKRDPIMKIVMKFGGTSVGDGKKIRHVAHLLKKYNEEGNQIVVVTSALGGVTDRLLENARLASTKGKVSLVKEFKTELT
NKHHEAVRDAIEDPNVAKDVLHILDLRIDELEKALIGICYLGELTNRSIDYISSYGERLAAPIISGAVRSLGIPSIEYTG
GEAGILTTSDYGNARPLEKTYELVSKRLGCRLDSHILVVTGFIGENEDGIITTLGRSGSDFSASILGAALKADEIWLWKE
VNGIMTTDPRIVPEAKTIPQISYAEAMELSYFGANVLHPRTIEPAMREHIPVRVKNTFDPEFPGTLVVSEKFQCKHVVKA
VSLIKNVALINISGAEMAGTIGTVARLFTALARAGVNIVMISQGSSESNLSFVVSEAHVEPALKALHAEFNREIIKEITS
DKNVCVVAVVGAGMAGTPGVAKRVFGALGNSMINIIMISQGSSQYNISFVVREDDAFAAVKTLHDEFELYNGNGIEKKL

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=463, Percent_Identity=33.9092872570194, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI1790455, Length=467, Percent_Identity=30.6209850107066, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI1790376, Length=426, Percent_Identity=25.5868544600939, Blast_Score=114, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6320893, Length=493, Percent_Identity=28.6004056795132, Blast_Score=188, Evalue=2e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 51915; Mature: 51915

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKRDPIMKIVMKFGGTSVGDGKKIRHVAHLLKKYNEEGNQIVVVTSALGGVTDRLLENA
CCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCC
RLASTKGKVSLVKEFKTELTNKHHEAVRDAIEDPNVAKDVLHILDLRIDELEKALIGICY
HHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHH
LGELTNRSIDYISSYGERLAAPIISGAVRSLGIPSIEYTGGEAGILTTSDYGNARPLEKT
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCEEEEECCCCCCCCHHHH
YELVSKRLGCRLDSHILVVTGFIGENEDGIITTLGRSGSDFSASILGAALKADEIWLWKE
HHHHHHHHCCCCCCCEEEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCEEEEEE
VNGIMTTDPRIVPEAKTIPQISYAEAMELSYFGANVLHPRTIEPAMREHIPVRVKNTFDP
CCCEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCEEEECCCCC
EFPGTLVVSEKFQCKHVVKAVSLIKNVALINISGAEMAGTIGTVARLFTALARAGVNIVM
CCCCEEEEECCHHHHHHHHHHHHHHCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCEEEE
ISQGSSESNLSFVVSEAHVEPALKALHAEFNREIIKEITSDKNVCVVAVVGAGMAGTPGV
EECCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHH
AKRVFGALGNSMINIIMISQGSSQYNISFVVREDDAFAAVKTLHDEFELYNGNGIEKKL
HHHHHHHHCCCEEEEEEEECCCCCEEEEEEEECCCHHHHHHHHHHHHHEECCCCCCCCC
>Mature Secondary Structure
MLKRDPIMKIVMKFGGTSVGDGKKIRHVAHLLKKYNEEGNQIVVVTSALGGVTDRLLENA
CCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCC
RLASTKGKVSLVKEFKTELTNKHHEAVRDAIEDPNVAKDVLHILDLRIDELEKALIGICY
HHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHH
LGELTNRSIDYISSYGERLAAPIISGAVRSLGIPSIEYTGGEAGILTTSDYGNARPLEKT
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCEEEEECCCCCCCCHHHH
YELVSKRLGCRLDSHILVVTGFIGENEDGIITTLGRSGSDFSASILGAALKADEIWLWKE
HHHHHHHHCCCCCCCEEEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCEEEEEE
VNGIMTTDPRIVPEAKTIPQISYAEAMELSYFGANVLHPRTIEPAMREHIPVRVKNTFDP
CCCEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCEEEECCCCC
EFPGTLVVSEKFQCKHVVKAVSLIKNVALINISGAEMAGTIGTVARLFTALARAGVNIVM
CCCCEEEEECCHHHHHHHHHHHHHHCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCEEEE
ISQGSSESNLSFVVSEAHVEPALKALHAEFNREIIKEITSDKNVCVVAVVGAGMAGTPGV
EECCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHH
AKRVFGALGNSMINIIMISQGSSQYNISFVVREDDAFAAVKTLHDEFELYNGNGIEKKL
HHHHHHHHCCCEEEEEEEECCCCCEEEEEEEECCCHHHHHHHHHHHHHEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]