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The map label for this gene is sepS [H]

Identifier: 21227485

GI number: 21227485

Start: 1662114

End: 1663733

Strand: Reverse

Name: sepS [H]

Synonym: MM_1383

Alternate gene names: 21227485

Gene position: 1663733-1662114 (Counterclockwise)

Preceding gene: 21227486

Following gene: 21227484

Centisome position: 40.62

GC content: 47.78

Gene sequence:

>1620_bases
ATGAAATTTGATCCAGAAAAAATAAAAAAAGATGCAAAGGAAAATTTTGACCATGCCTGGAACGAAGGCAAAAAAATGGT
CAAAACGCCTACACTTAACGAGCGCTACCCGAGGACAACCCTGAGATACGGGAAGGCTCACCCTGTTTACGATACAATCC
AGAAACTGAGAGAAGCCTATATGCGTATGGGTTTTGAAGAGATGATGAACCCTCTTATAGTTGATGAAAAAGAGGTCCAC
AAGCAGTTCGGAAGCGAGGCACTCGCAGTCCTTGACCGCTGTTTTTATCTTGCAGGCCTGCCCCGCCCCAATGTGGGGAT
TTCTGACGAACGCATCGCACAGATAACCGAAATCCTGGGTGATATAGGAGAAGAAGGCATAGACAAAATCAGGAAAGAAC
TTCACGCTTATAAGAAAGGAAAGATTGAAGGAGACGACCTGGTTCCCGAAATCTCTGCAGCCCTCGAAGTTTCGGATGCC
CTTGTAGCTGACATGATTGACAGGGTTTTCCCTGAGTTTAAAGAACTTATCCCCCAGGCAAGCACAAAAACCCTGCGCAG
TCATATGACAAGCGGCTGGTTCATTTCCCTCGGAGCCCTGCTTGAGAGAGAAGAGCCTCCTTTCCAATTCTTCTCAATAG
ACCGCTGTTTCAGGCGCGAACAGCAGGAAGATGCTTCAAGGCTTATGACCTATTATTCCGCTTCCTGTGTGATAATGGAT
GAAGATGTGACTGTAGACCATGGAAAAGCAGTATCTGAAGGGCTTCTCTCACAGTTCGGTTTCGAAAAGTTCCTTTTCAG
GCCGGACGAGAAGCGCAGCAAATATTATGTCCCTGACACCCAGACCGAGGTCTTCGCTTTCCATCCGAAACTGGTAGGTT
CTAACTCAAAATACTCTGATGGCTGGATCGAAGTCGCAACTTTCGGCATATATTCTCCTACAGCCCTTGCTGAATACGAT
ATTCCGTATCCCGTAATGAACCTCGGGCTCGGAGTAGAAAGGCTTGCCATGATCCTGCACGATGCGCCCGATGTCCGCTC
TTTGACCTACCCTCAGATCCCTCAGTACAGTGAGTGGGAAATGTCTGACAGTGGGCTTGCAAAGCAAGTTTTTGTAGACA
GAATGCCTGAAACCCCTGAAGGACAGGAAATTGCATCTGCAGTGGTTGCTCAGTGTGAATTGCACGGAGAAGAGCCAAGC
CCCTGTGAGTTCCCTGCCTGGGAAGGCGAGGTTTGCGGAAGAAAGGTTAAGGTTTCCGTAATCGAGCCAGAAGAAAATAC
AAAACTCTGCGGGCCTGCCGCGTTCAATGAAGTAGTTGCCTATCAGGGAGACATTCTGGGAATTCCAAACAACAAGAAAT
GGCAGAAAGCTTTTGAAAACCACTCTGCAAGGGCAGGCATACGCTTTATAGAGGCTTTTGCAGCCCAGGCTGCCAGAGAA
ATAGAAGAGGCTGCAATGTCCGGAGCAGATGAGCATATTGTCAGGATTCGGATTGTCAAGGTCCCCTCTGAAGTCAACCT
GAAGATAGGAGCTACTGCCCAGAGATATATCACAGGCAAAAACAAAAAGATAGATATCAGAGGCCCGGTCTTCACCTCGG
TAAAAGCGGAATTCGAGTAA

Upstream 100 bases:

>100_bases
CCGTACCGGCATTCAAACAGCTCAACATATACATATAAATGATAAAACCCTAAGGGTCTGATAGATCCGAATTCCAGTAA
TCAGTTACAAGAGGATATAT

Downstream 100 bases:

>100_bases
AATAAGCCGGCAAACAGAAATAATAATGCACAACGGAATGGGCTGTATGCAAAATGAAGGTGTCCCATCATCAGGATATT
CAGAAATCAGGAGACTGATG

Product: O-phosphoseryl-tRNA synthetase

Products: NA

Alternate protein names: SepRS; Non-canonical O-phosphoseryl-tRNA(Cys) synthetase; O-phosphoserine--tRNA(Cys) ligase; O-phosphoserine--tRNA ligase [H]

Number of amino acids: Translated: 539; Mature: 539

Protein sequence:

>539_residues
MKFDPEKIKKDAKENFDHAWNEGKKMVKTPTLNERYPRTTLRYGKAHPVYDTIQKLREAYMRMGFEEMMNPLIVDEKEVH
KQFGSEALAVLDRCFYLAGLPRPNVGISDERIAQITEILGDIGEEGIDKIRKELHAYKKGKIEGDDLVPEISAALEVSDA
LVADMIDRVFPEFKELIPQASTKTLRSHMTSGWFISLGALLEREEPPFQFFSIDRCFRREQQEDASRLMTYYSASCVIMD
EDVTVDHGKAVSEGLLSQFGFEKFLFRPDEKRSKYYVPDTQTEVFAFHPKLVGSNSKYSDGWIEVATFGIYSPTALAEYD
IPYPVMNLGLGVERLAMILHDAPDVRSLTYPQIPQYSEWEMSDSGLAKQVFVDRMPETPEGQEIASAVVAQCELHGEEPS
PCEFPAWEGEVCGRKVKVSVIEPEENTKLCGPAAFNEVVAYQGDILGIPNNKKWQKAFENHSARAGIRFIEAFAAQAARE
IEEAAMSGADEHIVRIRIVKVPSEVNLKIGATAQRYITGKNKKIDIRGPVFTSVKAEFE

Sequences:

>Translated_539_residues
MKFDPEKIKKDAKENFDHAWNEGKKMVKTPTLNERYPRTTLRYGKAHPVYDTIQKLREAYMRMGFEEMMNPLIVDEKEVH
KQFGSEALAVLDRCFYLAGLPRPNVGISDERIAQITEILGDIGEEGIDKIRKELHAYKKGKIEGDDLVPEISAALEVSDA
LVADMIDRVFPEFKELIPQASTKTLRSHMTSGWFISLGALLEREEPPFQFFSIDRCFRREQQEDASRLMTYYSASCVIMD
EDVTVDHGKAVSEGLLSQFGFEKFLFRPDEKRSKYYVPDTQTEVFAFHPKLVGSNSKYSDGWIEVATFGIYSPTALAEYD
IPYPVMNLGLGVERLAMILHDAPDVRSLTYPQIPQYSEWEMSDSGLAKQVFVDRMPETPEGQEIASAVVAQCELHGEEPS
PCEFPAWEGEVCGRKVKVSVIEPEENTKLCGPAAFNEVVAYQGDILGIPNNKKWQKAFENHSARAGIRFIEAFAAQAARE
IEEAAMSGADEHIVRIRIVKVPSEVNLKIGATAQRYITGKNKKIDIRGPVFTSVKAEFE
>Mature_539_residues
MKFDPEKIKKDAKENFDHAWNEGKKMVKTPTLNERYPRTTLRYGKAHPVYDTIQKLREAYMRMGFEEMMNPLIVDEKEVH
KQFGSEALAVLDRCFYLAGLPRPNVGISDERIAQITEILGDIGEEGIDKIRKELHAYKKGKIEGDDLVPEISAALEVSDA
LVADMIDRVFPEFKELIPQASTKTLRSHMTSGWFISLGALLEREEPPFQFFSIDRCFRREQQEDASRLMTYYSASCVIMD
EDVTVDHGKAVSEGLLSQFGFEKFLFRPDEKRSKYYVPDTQTEVFAFHPKLVGSNSKYSDGWIEVATFGIYSPTALAEYD
IPYPVMNLGLGVERLAMILHDAPDVRSLTYPQIPQYSEWEMSDSGLAKQVFVDRMPETPEGQEIASAVVAQCELHGEEPS
PCEFPAWEGEVCGRKVKVSVIEPEENTKLCGPAAFNEVVAYQGDILGIPNNKKWQKAFENHSARAGIRFIEAFAAQAARE
IEEAAMSGADEHIVRIRIVKVPSEVNLKIGATAQRYITGKNKKIDIRGPVFTSVKAEFE

Specific function: Catalyzes the attachment of O-phosphoserine (Sep) to tRNA(Cys) [H]

COG id: COG2024

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit (archaeal type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. O-phosphoseryl-tRNA(Cys) synthetase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758340, Length=214, Percent_Identity=28.5046728971963, Blast_Score=73, Evalue=6e-13,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR005246
- InterPro:   IPR002319 [H]

Pfam domain/function: PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.27 [H]

Molecular weight: Translated: 60906; Mature: 60906

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFDPEKIKKDAKENFDHAWNEGKKMVKTPTLNERYPRTTLRYGKAHPVYDTIQKLREAY
CCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHH
MRMGFEEMMNPLIVDEKEVHKQFGSEALAVLDRCFYLAGLPRPNVGISDERIAQITEILG
HHCCHHHHCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
DIGEEGIDKIRKELHAYKKGKIEGDDLVPEISAALEVSDALVADMIDRVFPEFKELIPQA
HHCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
STKTLRSHMTSGWFISLGALLEREEPPFQFFSIDRCFRREQQEDASRLMTYYSASCVIMD
HHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEE
EDVTVDHGKAVSEGLLSQFGFEKFLFRPDEKRSKYYVPDTQTEVFAFHPKLVGSNSKYSD
CCCCCCCCHHHHHHHHHHHCCHHHHCCCCCCCCCEECCCCCCEEEEECCHHCCCCCCCCC
GWIEVATFGIYSPTALAEYDIPYPVMNLGLGVERLAMILHDAPDVRSLTYPQIPQYSEWE
CEEEEEEECCCCCCHHCCCCCCCHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
MSDSGLAKQVFVDRMPETPEGQEIASAVVAQCELHGEEPSPCEFPAWEGEVCGRKVKVSV
CCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEE
IEPEENTKLCGPAAFNEVVAYQGDILGIPNNKKWQKAFENHSARAGIRFIEAFAAQAARE
ECCCCCCCCCCHHHHHHHHHHCCCEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
IEEAAMSGADEHIVRIRIVKVPSEVNLKIGATAQRYITGKNKKIDIRGPVFTSVKAEFE
HHHHHHCCCCCEEEEEEEEECCCCCCEEECCCHHHHCCCCCCEEEECCCEEECCCCCCC
>Mature Secondary Structure
MKFDPEKIKKDAKENFDHAWNEGKKMVKTPTLNERYPRTTLRYGKAHPVYDTIQKLREAY
CCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHH
MRMGFEEMMNPLIVDEKEVHKQFGSEALAVLDRCFYLAGLPRPNVGISDERIAQITEILG
HHCCHHHHCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
DIGEEGIDKIRKELHAYKKGKIEGDDLVPEISAALEVSDALVADMIDRVFPEFKELIPQA
HHCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
STKTLRSHMTSGWFISLGALLEREEPPFQFFSIDRCFRREQQEDASRLMTYYSASCVIMD
HHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEE
EDVTVDHGKAVSEGLLSQFGFEKFLFRPDEKRSKYYVPDTQTEVFAFHPKLVGSNSKYSD
CCCCCCCCHHHHHHHHHHHCCHHHHCCCCCCCCCEECCCCCCEEEEECCHHCCCCCCCCC
GWIEVATFGIYSPTALAEYDIPYPVMNLGLGVERLAMILHDAPDVRSLTYPQIPQYSEWE
CEEEEEEECCCCCCHHCCCCCCCHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
MSDSGLAKQVFVDRMPETPEGQEIASAVVAQCELHGEEPSPCEFPAWEGEVCGRKVKVSV
CCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEE
IEPEENTKLCGPAAFNEVVAYQGDILGIPNNKKWQKAFENHSARAGIRFIEAFAAQAARE
ECCCCCCCCCCHHHHHHHHHHCCCEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
IEEAAMSGADEHIVRIRIVKVPSEVNLKIGATAQRYITGKNKKIDIRGPVFTSVKAEFE
HHHHHHCCCCCEEEEEEEEECCCCCCEEECCCHHHHCCCCCCEEEECCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]