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The map label for this gene is mttC [H]

Identifier: 21227157

GI number: 21227157

Start: 1237168

End: 1237701

Strand: Direct

Name: mttC [H]

Synonym: MM_1055

Alternate gene names: 21227157

Gene position: 1237168-1237701 (Clockwise)

Preceding gene: 21227156

Following gene: 21227160

Centisome position: 30.2

GC content: 47.75

Gene sequence:

>534_bases
TTGAGTTTACTGGTCCTGTTAATTCTGGGGATAGTTGCTGCTCAATTCATCTTCCCTGTGTCGGCCGGAGATCCGGGCGG
AAAGGAGAGAGGTAATGTGGGAACTGTCGTAATCGCTACTGTTGAAGGGGATTCCCACACTTACGGTAAGGACAGTATTG
CAGCCGCTTTTGAAAAAGAGGGCTTTGAAGTAATTAACCTTGGAGAGGGAGTCTCATCTGAAAGATTTGCTGAAGCCGCC
AAAGAACACGAAGCAGATTTTGTATTCAGTTCGGCATCCATGAGCACAACCATGATCCGGCAGATACAGATTGAAGAGCA
GCTTAAAAATGCCGGAATACGGGATAAAGTCGTAACAGGTGTGGGGGGATCGCTGATAACTCAGGAATGGGCTGACCGGA
TAGGAGCTGACATCTACGCTTCAGGTTCGGAAGACGCGGTCTTAAAAGCAAAATCGGCTCTCCTGAACAGCAGCACCCAG
AAAACCCATATTTCGGCTGATGAAAATACAAATTGCGGAAGCTGCCATGGATAA

Upstream 100 bases:

>100_bases
AAAGGTGCAGGAGGAACAGGTAATTCCGGAGAAAGAAAAAGTCAGAAGGAAGTCAAAAATCTGGAGAAAGAAAGGAGAGA
AAAATATGTGCGTTAAAAAA

Downstream 100 bases:

>100_bases
ACCCACAGAACAAAAATTTTGAACAGGAAAATTGGGAGACGTTAACTCTCCCAATCAAATTGTTTAGATAAAATGTAGTA
ATAAATCGGGCCAGCCGGGA

Product: trimethylamine corrinoid protein

Products: L-Methionine; 5,6,7,8-Tetrahydrofolate [C]

Alternate protein names: TCP 2 [H]

Number of amino acids: Translated: 177; Mature: 176

Protein sequence:

>177_residues
MSLLVLLILGIVAAQFIFPVSAGDPGGKERGNVGTVVIATVEGDSHTYGKDSIAAAFEKEGFEVINLGEGVSSERFAEAA
KEHEADFVFSSASMSTTMIRQIQIEEQLKNAGIRDKVVTGVGGSLITQEWADRIGADIYASGSEDAVLKAKSALLNSSTQ
KTHISADENTNCGSCHG

Sequences:

>Translated_177_residues
MSLLVLLILGIVAAQFIFPVSAGDPGGKERGNVGTVVIATVEGDSHTYGKDSIAAAFEKEGFEVINLGEGVSSERFAEAA
KEHEADFVFSSASMSTTMIRQIQIEEQLKNAGIRDKVVTGVGGSLITQEWADRIGADIYASGSEDAVLKAKSALLNSSTQ
KTHISADENTNCGSCHG
>Mature_176_residues
SLLVLLILGIVAAQFIFPVSAGDPGGKERGNVGTVVIATVEGDSHTYGKDSIAAAFEKEGFEVINLGEGVSSERFAEAAK
EHEADFVFSSASMSTTMIRQIQIEEQLKNAGIRDKVVTGVGGSLITQEWADRIGADIYASGSEDAVLKAKSALLNSSTQK
THISADENTNCGSCHG

Specific function: Acts probably as a methyl group carrier between mttB and either mtbA or mtaA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding N-terminal domain [H]

Homologues:

None

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR003759
- InterPro:   IPR012741 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2 [H]

EC number: 2.1.1.13 [C]

Molecular weight: Translated: 18565; Mature: 18434

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLLVLLILGIVAAQFIFPVSAGDPGGKERGNVGTVVIATVEGDSHTYGKDSIAAAFEKE
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHC
GFEVINLGEGVSSERFAEAAKEHEADFVFSSASMSTTMIRQIQIEEQLKNAGIRDKVVTG
CCEEEECCCCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHC
VGGSLITQEWADRIGADIYASGSEDAVLKAKSALLNSSTQKTHISADENTNCGSCHG
CCCHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCC
>Mature Secondary Structure 
SLLVLLILGIVAAQFIFPVSAGDPGGKERGNVGTVVIATVEGDSHTYGKDSIAAAFEKE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHC
GFEVINLGEGVSSERFAEAAKEHEADFVFSSASMSTTMIRQIQIEEQLKNAGIRDKVVTG
CCEEEECCCCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHC
VGGSLITQEWADRIGADIYASGSEDAVLKAKSALLNSSTQKTHISADENTNCGSCHG
CCCHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: ATP; Cobinamide; S-adenosyl-L-methionine [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 500-780 [C]

Specific activity: 3.8

Km value (mM): 0.035 {S-methyltetrahydrofolate}} 0.0016 {S-adenosyl-L-methionine}} 0.00065 {S-adenosyl-L-methionine}} 0.06 {methyltetrahydrofolate}} 0.03 {methyltetrahydrofolate}} 0.016 {homocysteine}} [C]

Substrates: 5-Methyltetrahydrofolate; L-Homocysteine [C]

Specific reaction: 5-Methyltetrahydrofolate + L-Homocysteine --> L-Methionine + 5,6,7,8-Tetrahydrofolate [C]

General reaction: Methyl group transfer [C]

Inhibitor: 5, 6-Dimethyl benzimidazole; alpha-Ribazole 3'-phosphate; Be2+; Cobinamide; Propyl iodide; Sr2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12125824 [H]