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The map label for this gene is ogt

Identifier: 21227122

GI number: 21227122

Start: 1193212

End: 1193688

Strand: Reverse

Name: ogt

Synonym: MM_1020

Alternate gene names: 21227122

Gene position: 1193688-1193212 (Counterclockwise)

Preceding gene: 161485678

Following gene: 21227121

Centisome position: 29.14

GC content: 41.72

Gene sequence:

>477_bases
ATGTATTATCACATAATCAAATCCCCTATCTACCCAATTCTTCTCGCAGGAGATGAAAAAGGGCTGAAACATCTTATTTT
CTTGAAAGACGAAAGAAAAGCTAAAATCCCCAATGACTGGGTAGAAAATAAAGATTTTTTCAGGGAAGTCTCTAACCAGC
TTGAAGCTTATTTTTCGGGAAAACTCAAGACTTTTGATGTAAAGCTGGCTCCGCAAGGAACTGAATTCCAGAAATCCGTC
TGGAAAGCTTTATGTGAAATCCCTTGTGGAGAGACCCGAACTTACGGGGAGATCGCAAAAAGAATTCAGAACCCGAAGGC
TTACAGGGCTGTCGGGCTTGCAAACAACAGGAATCCGATTGCAATAATTGTGCCCTGCCACAGGGTTATCGGGGCGAATG
GAAAACTTACGGGCTACGCGAGCGGGCTGGATATAAAAGAGTTTCTACTAAAACTTGAAGAAAACAATTTGAAATAA

Upstream 100 bases:

>100_bases
GTAAGGGGCAGGTTAGTGAATAAACATTTCTACCATTAGCCATAGAATTTGTTCTACTATTAATCATAGAATTATCTGTA
ATCGAGAGGAGAAAATAGAA

Downstream 100 bases:

>100_bases
GAGAATTACATAAATTTTATTTAAGTTCTCTGCAACACCTATCCAGTTCTCTTTGAATACTGTCAATTTTTTCTAATTGC
TCTCTTTGAATGCTGTAAAT

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase

Number of amino acids: Translated: 158; Mature: 158

Protein sequence:

>158_residues
MYYHIIKSPIYPILLAGDEKGLKHLIFLKDERKAKIPNDWVENKDFFREVSNQLEAYFSGKLKTFDVKLAPQGTEFQKSV
WKALCEIPCGETRTYGEIAKRIQNPKAYRAVGLANNRNPIAIIVPCHRVIGANGKLTGYASGLDIKEFLLKLEENNLK

Sequences:

>Translated_158_residues
MYYHIIKSPIYPILLAGDEKGLKHLIFLKDERKAKIPNDWVENKDFFREVSNQLEAYFSGKLKTFDVKLAPQGTEFQKSV
WKALCEIPCGETRTYGEIAKRIQNPKAYRAVGLANNRNPIAIIVPCHRVIGANGKLTGYASGLDIKEFLLKLEENNLK
>Mature_158_residues
MYYHIIKSPIYPILLAGDEKGLKHLIFLKDERKAKIPNDWVENKDFFREVSNQLEAYFSGKLKTFDVKLAPQGTEFQKSV
WKALCEIPCGETRTYGEIAKRIQNPKAYRAVGLANNRNPIAIIVPCHRVIGANGKLTGYASGLDIKEFLLKLEENNLK

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family

Homologues:

Organism=Homo sapiens, GI197304670, Length=170, Percent_Identity=31.7647058823529, Blast_Score=77, Evalue=7e-15,
Organism=Escherichia coli, GI1787596, Length=105, Percent_Identity=52.3809523809524, Blast_Score=117, Evalue=4e-28,
Organism=Escherichia coli, GI1788542, Length=111, Percent_Identity=41.4414414414414, Blast_Score=91, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI115533070, Length=85, Percent_Identity=50.5882352941176, Blast_Score=85, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI115533068, Length=85, Percent_Identity=50.5882352941176, Blast_Score=84, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6320001, Length=84, Percent_Identity=50, Blast_Score=89, Evalue=3e-19,
Organism=Drosophila melanogaster, GI17137554, Length=114, Percent_Identity=38.5964912280702, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): OGT_METMA (Q8PY44)

Other databases:

- EMBL:   AE008384
- RefSeq:   NP_633044.1
- ProteinModelPortal:   Q8PY44
- SMR:   Q8PY44
- GeneID:   1479362
- GenomeReviews:   AE008384_GR
- KEGG:   mma:MM_1020
- NMPDR:   fig|192952.1.peg.1020
- HOGENOM:   HBG752398
- OMA:   AVGMANS
- ProtClustDB:   PRK00901
- BioCyc:   MMAZ192952:MM1020-MONOMER
- BRENDA:   2.1.1.63
- GO:   GO:0005737
- HAMAP:   MF_00772
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PANTHER:   PTHR10815
- TIGRFAMs:   TIGR00589

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N; SSF46767 MethylDNA_cys_mtrans_DNA_bd

EC number: =2.1.1.63

Molecular weight: Translated: 17996; Mature: 17996

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: PS00374 MGMT

Important sites: ACT_SITE 126-126

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYYHIIKSPIYPILLAGDEKGLKHLIFLKDERKAKIPNDWVENKDFFREVSNQLEAYFSG
CEEEEECCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCHHCCHHHHHHHHHHHHHHHCC
KLKTFDVKLAPQGTEFQKSVWKALCEIPCGETRTYGEIAKRIQNPKAYRAVGLANNRNPI
CEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHEEEECCCCCCCE
AIIVPCHRVIGANGKLTGYASGLDIKEFLLKLEENNLK
EEEEECHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MYYHIIKSPIYPILLAGDEKGLKHLIFLKDERKAKIPNDWVENKDFFREVSNQLEAYFSG
CEEEEECCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCHHCCHHHHHHHHHHHHHHHCC
KLKTFDVKLAPQGTEFQKSVWKALCEIPCGETRTYGEIAKRIQNPKAYRAVGLANNRNPI
CEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHEEEECCCCCCCE
AIIVPCHRVIGANGKLTGYASGLDIKEFLLKLEENNLK
EEEEECHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12125824