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The map label for this gene is thiC [H]

Identifier: 161485678

GI number: 161485678

Start: 1197056

End: 1198342

Strand: Reverse

Name: thiC [H]

Synonym: MM_1025

Alternate gene names: 161485678

Gene position: 1198342-1197056 (Counterclockwise)

Preceding gene: 21227128

Following gene: 21227122

Centisome position: 29.25

GC content: 52.68

Gene sequence:

>1287_bases
ATGACAATTGTGGAAGATGCAAAAAAAGGAATTATCACCGAAGAGATGAAGATCGTCGCAAAGGATGAAGGACTTGACCC
TGAATTCATCCGCCGCGGTATTGCAGCCGGAAGAATCGTTATCCCCACCTCTCCTTACAGGCAGGTAAAGCTCTGTGGAC
TCGGAGAAGGTCTGAGGACCAAAATCAACGCTTCTATCGGTGTATCTTCTGACATCGTTGACGACGAAATGGAAGTCAAG
AAGGCAATCGCTGCCGAGAAGGCAGGTGCCGACACACTCATGGAACTGGGTACCGGTGGAGACTTCCTCGGGATCAGGAA
AAAAGTAATTGACAGCATCTCCCTTTCTGTCGGGTCTGTTCCTCTCTACCAGGCTTTCATCGAAGCCGCAAGGAAGTACG
GTTCAATTGTGCACATGACTGAAGACGAGCTCTTCAAGGCAACTGAAGACCAGGCAAAGCTCGGGACCAACTTCATGGCA
ATCCACACAGGTGTAAACAACATCACCCTTGACAGGCTTAAAGCACACGGAAGGTATGGCGGTCTCTGCTCCCGCGGCGG
TGCCTTCATGAGCGCCTGGATGATGCACAACGAAAAGGAAAACCCGCTTTACTCTCAGTTCGACTACCTTGTTGAGATCC
TCAAGGAACATGAAGTAGTTCTCTCAACCGGGAACGGGATGCGCGCAGGAGCAACCCACGACGCAACAGACCGCGCCCAG
ATTCAGGAACTGATCATCAACACAGAACTCGGCCAGAGAGCCCACGATGCAGGCCTGCAGGTCATCATCGAAGGTCCAGG
TCACATCCCACTCGACCAGATCGAGACCAATGTCAAGATCATGAAGGAAATGAGCGGTCACAAGCCCTTCTACATGCTCG
GACCCCTCGCAACCGACATCGCCCCCGGCTACGACCACATCGTAACCGCAATCGGCGCATCCCTTTCAGCTTCCTATGGC
TGTGACTTCCTCTGCTACGTGACCCCTGCAGAGCACCTTGCCCTCCCCAACATCGAAGATGTTATCACAGGTGTCAAGAC
CTCCAAGATCGCAGCTCACATAGGGGACATGGTAAAGTATCCTGAAAGAGCAAGACAGTGGGACCTTGACATGGGCCGTG
CCAGGAGAGACCTGGACTGGGAAAAGATGTACTCTCTCGCCCTGGACCCCGAACACGCCAGAGCAGTCAGGAACAGCAGG
GCACCTGAAGACTCAGATGCATGCACAATGTGCGGAAACTTCTGCGCCCTCAAGATCGTTAACCAGAACTACAACCTTGC
AAAATAA

Upstream 100 bases:

>100_bases
ATATAATTACTTTCCTTTTATGTTCTAACTCACAATCCCTTTACTTACATATGAACTCTGACGATAATCTCATTTACAAA
TGAAAGTTGAGGTAATAAAG

Downstream 100 bases:

>100_bases
ACTTAAAAAAATAAAGTAAGGCTTTAGAGCTTTACTTTTTCACCTTTTCTTTTATCCTCATATTTTCGATTTTTATTCTG
TTTTTCCCTATTTTTTCCGT

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase 1; HMP-P synthase 1; HMP-phosphate synthase 1; HMPP synthase 1; Thiamine biosynthesis protein thiC 1 [H]

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MTIVEDAKKGIITEEMKIVAKDEGLDPEFIRRGIAAGRIVIPTSPYRQVKLCGLGEGLRTKINASIGVSSDIVDDEMEVK
KAIAAEKAGADTLMELGTGGDFLGIRKKVIDSISLSVGSVPLYQAFIEAARKYGSIVHMTEDELFKATEDQAKLGTNFMA
IHTGVNNITLDRLKAHGRYGGLCSRGGAFMSAWMMHNEKENPLYSQFDYLVEILKEHEVVLSTGNGMRAGATHDATDRAQ
IQELIINTELGQRAHDAGLQVIIEGPGHIPLDQIETNVKIMKEMSGHKPFYMLGPLATDIAPGYDHIVTAIGASLSASYG
CDFLCYVTPAEHLALPNIEDVITGVKTSKIAAHIGDMVKYPERARQWDLDMGRARRDLDWEKMYSLALDPEHARAVRNSR
APEDSDACTMCGNFCALKIVNQNYNLAK

Sequences:

>Translated_428_residues
MTIVEDAKKGIITEEMKIVAKDEGLDPEFIRRGIAAGRIVIPTSPYRQVKLCGLGEGLRTKINASIGVSSDIVDDEMEVK
KAIAAEKAGADTLMELGTGGDFLGIRKKVIDSISLSVGSVPLYQAFIEAARKYGSIVHMTEDELFKATEDQAKLGTNFMA
IHTGVNNITLDRLKAHGRYGGLCSRGGAFMSAWMMHNEKENPLYSQFDYLVEILKEHEVVLSTGNGMRAGATHDATDRAQ
IQELIINTELGQRAHDAGLQVIIEGPGHIPLDQIETNVKIMKEMSGHKPFYMLGPLATDIAPGYDHIVTAIGASLSASYG
CDFLCYVTPAEHLALPNIEDVITGVKTSKIAAHIGDMVKYPERARQWDLDMGRARRDLDWEKMYSLALDPEHARAVRNSR
APEDSDACTMCGNFCALKIVNQNYNLAK
>Mature_427_residues
TIVEDAKKGIITEEMKIVAKDEGLDPEFIRRGIAAGRIVIPTSPYRQVKLCGLGEGLRTKINASIGVSSDIVDDEMEVKK
AIAAEKAGADTLMELGTGGDFLGIRKKVIDSISLSVGSVPLYQAFIEAARKYGSIVHMTEDELFKATEDQAKLGTNFMAI
HTGVNNITLDRLKAHGRYGGLCSRGGAFMSAWMMHNEKENPLYSQFDYLVEILKEHEVVLSTGNGMRAGATHDATDRAQI
QELIINTELGQRAHDAGLQVIIEGPGHIPLDQIETNVKIMKEMSGHKPFYMLGPLATDIAPGYDHIVTAIGASLSASYGC
DFLCYVTPAEHLALPNIEDVITGVKTSKIAAHIGDMVKYPERARQWDLDMGRARRDLDWEKMYSLALDPEHARAVRNSRA
PEDSDACTMCGNFCALKIVNQNYNLAK

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=465, Percent_Identity=37.4193548387097, Blast_Score=286, Evalue=2e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 46884; Mature: 46753

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIVEDAKKGIITEEMKIVAKDEGLDPEFIRRGIAAGRIVIPTSPYRQVKLCGLGEGLRT
CCCHHHHHCCCCHHHHEEEECCCCCCHHHHHHHHCCCEEEECCCCCCEEEEECCCCCHHH
KINASIGVSSDIVDDEMEVKKAIAAEKAGADTLMELGTGGDFLGIRKKVIDSISLSVGSV
EECCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCC
PLYQAFIEAARKYGSIVHMTEDELFKATEDQAKLGTNFMAIHTGVNNITLDRLKAHGRYG
HHHHHHHHHHHHCCCEEEECHHHHHHHCCHHHHHCCCEEEEECCCCCEEHHHHHHCCCCC
GLCSRGGAFMSAWMMHNEKENPLYSQFDYLVEILKEHEVVLSTGNGMRAGATHDATDRAQ
CCHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHH
IQELIINTELGQRAHDAGLQVIIEGPGHIPLDQIETNVKIMKEMSGHKPFYMLGPLATDI
HHHHHHHHHHCCHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEECCHHHHC
APGYDHIVTAIGASLSASYGCDFLCYVTPAEHLALPNIEDVITGVKTSKIAAHIGDMVKY
CCCHHHHHHHHHHHHCCCCCCCEEEEECCHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHC
PERARQWDLDMGRARRDLDWEKMYSLALDPEHARAVRNSRAPEDSDACTMCGNFCALKIV
CHHHHHCCCCHHHHHCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHCCEEEEEEE
NQNYNLAK
CCCCCCCC
>Mature Secondary Structure 
TIVEDAKKGIITEEMKIVAKDEGLDPEFIRRGIAAGRIVIPTSPYRQVKLCGLGEGLRT
CCHHHHHCCCCHHHHEEEECCCCCCHHHHHHHHCCCEEEECCCCCCEEEEECCCCCHHH
KINASIGVSSDIVDDEMEVKKAIAAEKAGADTLMELGTGGDFLGIRKKVIDSISLSVGSV
EECCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCC
PLYQAFIEAARKYGSIVHMTEDELFKATEDQAKLGTNFMAIHTGVNNITLDRLKAHGRYG
HHHHHHHHHHHHCCCEEEECHHHHHHHCCHHHHHCCCEEEEECCCCCEEHHHHHHCCCCC
GLCSRGGAFMSAWMMHNEKENPLYSQFDYLVEILKEHEVVLSTGNGMRAGATHDATDRAQ
CCHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHH
IQELIINTELGQRAHDAGLQVIIEGPGHIPLDQIETNVKIMKEMSGHKPFYMLGPLATDI
HHHHHHHHHHCCHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEECCHHHHC
APGYDHIVTAIGASLSASYGCDFLCYVTPAEHLALPNIEDVITGVKTSKIAAHIGDMVKY
CCCHHHHHHHHHHHHCCCCCCCEEEEECCHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHC
PERARQWDLDMGRARRDLDWEKMYSLALDPEHARAVRNSRAPEDSDACTMCGNFCALKIV
CHHHHHCCCCHHHHHCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHCCEEEEEEE
NQNYNLAK
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA