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The map label for this gene is cbiF [H]

Identifier: 21227100

GI number: 21227100

Start: 1172630

End: 1173358

Strand: Reverse

Name: cbiF [H]

Synonym: MM_0998

Alternate gene names: 21227100

Gene position: 1173358-1172630 (Counterclockwise)

Preceding gene: 21227101

Following gene: 21227099

Centisome position: 28.64

GC content: 48.29

Gene sequence:

>729_bases
ATGGAAAGAAAAGTATATTTTGTAGGGGCAGGGCCTGGAAACCCCAAACTGATTACCGTTCTGGGACGTGAAATGCTCGA
AAAAGCAGACCTTGTTATGTATGCGGGCTCCCTGGTAAACCCTGAAGTCCTTAATTACACTAAAGGGGAAACTGTGGACA
GCTACGGGCTTACCCTTGACGAAACCACGAAAATAATCGCCGATGCGGTGGATGCAGGGAAGTTTGTGGTGAGGCTCCAT
AGCGGAGACCCCTCTCTTTACGGGTCTGTCATAGAACAGATGGAAGAGCTGAGAAAATACGATATTGAGGTAGAAAGGGT
TGCAGGTGTCTCCTCGGTTTTTGCAAGTGCAGCAGCTCTCGGAACCCAGCTTACTCTTAACGGCGTTTCCGATACCCTTA
TCATTACCCGCCCTGCTGGAAAGACTCTGGAAAAAGACCTTATCCCCGAGCTTTCTGCTTATAACACCACAATGGCAATT
TTCCTAGGCACCCAGAAGATCAGGGAAATAATGGACAAAGTCCGCTGCCCAAAAGATACTCCCGTTGCAGTTGTTTTCCA
CGCATCCTGGGAAGACGAAGAAGTTATTGCAGGGACTGTGGAAGATATCGCAGATAAGGTCAAAGATGCAGGAATTACAC
GCTCGGCAATGATTATAATCGGCGGAGTTGTCGACCCTAAAAACTACAGGAGGTCCTATCTATACGGAGTGGCCCAGGAA
CCGCTGTAA

Upstream 100 bases:

>100_bases
CTGTAGACAATTGAAAACCTCAATATGCAAATTAACCTCCTATAATCAAATTAATTCACTATAATTCTGAAATACTCAGG
AAAGCAAAGGGAATGGTCAG

Downstream 100 bases:

>100_bases
TTACCTTTGAAAGGAACAGGGAAATTGCATCAAGGGTAGCAGAGCATCTTGAAGCCGACCTCCTTCTCTACGAAAAGGGC
ATCTTCAGGAAAGCCTTTGA

Product: precorrin-4 C11-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 5

Alternate protein names: Cobalt-precorrin-3 methylase [H]

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MERKVYFVGAGPGNPKLITVLGREMLEKADLVMYAGSLVNPEVLNYTKGETVDSYGLTLDETTKIIADAVDAGKFVVRLH
SGDPSLYGSVIEQMEELRKYDIEVERVAGVSSVFASAAALGTQLTLNGVSDTLIITRPAGKTLEKDLIPELSAYNTTMAI
FLGTQKIREIMDKVRCPKDTPVAVVFHASWEDEEVIAGTVEDIADKVKDAGITRSAMIIIGGVVDPKNYRRSYLYGVAQE
PL

Sequences:

>Translated_242_residues
MERKVYFVGAGPGNPKLITVLGREMLEKADLVMYAGSLVNPEVLNYTKGETVDSYGLTLDETTKIIADAVDAGKFVVRLH
SGDPSLYGSVIEQMEELRKYDIEVERVAGVSSVFASAAALGTQLTLNGVSDTLIITRPAGKTLEKDLIPELSAYNTTMAI
FLGTQKIREIMDKVRCPKDTPVAVVFHASWEDEEVIAGTVEDIADKVKDAGITRSAMIIIGGVVDPKNYRRSYLYGVAQE
PL
>Mature_242_residues
MERKVYFVGAGPGNPKLITVLGREMLEKADLVMYAGSLVNPEVLNYTKGETVDSYGLTLDETTKIIADAVDAGKFVVRLH
SGDPSLYGSVIEQMEELRKYDIEVERVAGVSSVFASAAALGTQLTLNGVSDTLIITRPAGKTLEKDLIPELSAYNTTMAI
FLGTQKIREIMDKVRCPKDTPVAVVFHASWEDEEVIAGTVEDIADKVKDAGITRSAMIIIGGVVDPKNYRRSYLYGVAQE
PL

Specific function: Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5 [H]

COG id: COG2875

COG function: function code H; Precorrin-4 methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=232, Percent_Identity=27.1551724137931, Blast_Score=80, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006362
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.133

Molecular weight: Translated: 26334; Mature: 26334

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERKVYFVGAGPGNPKLITVLGREMLEKADLVMYAGSLVNPEVLNYTKGETVDSYGLTLD
CCCEEEEEECCCCCCCEEEEHHHHHHHHHHHEEECCCCCCHHHHCCCCCCCCCCCCCCHH
ETTKIIADAVDAGKFVVRLHSGDPSLYGSVIEQMEELRKYDIEVERVAGVSSVFASAAAL
HHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
GTQLTLNGVSDTLIITRPAGKTLEKDLIPELSAYNTTMAIFLGTQKIREIMDKVRCPKDT
CCEEEECCCCCEEEEECCCCCCHHHHHCCCHHHCCCEEEEEECHHHHHHHHHHHCCCCCC
PVAVVFHASWEDEEVIAGTVEDIADKVKDAGITRSAMIIIGGVVDPKNYRRSYLYGVAQE
CEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHCCCCC
PL
CC
>Mature Secondary Structure
MERKVYFVGAGPGNPKLITVLGREMLEKADLVMYAGSLVNPEVLNYTKGETVDSYGLTLD
CCCEEEEEECCCCCCCEEEEHHHHHHHHHHHEEECCCCCCHHHHCCCCCCCCCCCCCCHH
ETTKIIADAVDAGKFVVRLHSGDPSLYGSVIEQMEELRKYDIEVERVAGVSSVFASAAAL
HHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
GTQLTLNGVSDTLIITRPAGKTLEKDLIPELSAYNTTMAIFLGTQKIREIMDKVRCPKDT
CCEEEECCCCCEEEEECCCCCCHHHHHCCCHHHCCCEEEEEECHHHHHHHHHHHCCCCCC
PVAVVFHASWEDEEVIAGTVEDIADKVKDAGITRSAMIIIGGVVDPKNYRRSYLYGVAQE
CEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHCCCCC
PL
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 4

Specific reaction: S-adenosyl-L-methionine + precorrin-4 = S-adenosyl-L-homocysteine + precorrin-5

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]