Click here to switch to the map view.

The map label for this gene is purB [H]

Identifier: 21227043

GI number: 21227043

Start: 1115096

End: 1116454

Strand: Reverse

Name: purB [H]

Synonym: MM_0941

Alternate gene names: 21227043

Gene position: 1116454-1115096 (Counterclockwise)

Preceding gene: 21227044

Following gene: 21227041

Centisome position: 27.25

GC content: 46.8

Gene sequence:

>1359_bases
GTGGTGATTGATATGGCTATTCATCCTATAGAATACCGGTACGGTACAGCAGAAATGAAATATGTGTGGAGTCAGGAAAA
CAGACTTGCTAAGATCTTGCAGACCGAGGCAGCTCTTGCCCGGGCAGAAGCGGATATGGGGCTTATTCCCGCAGAAGCGG
CTGAAATTATATTCGAAAGCATTGCCTCTGTAAAAGCCGAGAGAGTCGATGAAATAGAATCCGAGATCCACCATGACATG
ATGGCTGTCGTTGTTGCAATTTCAGAGATGTGCAGGGATGACGCAGGAAAATGGGTTCACTTCGGAGCCACTTCAAACGA
TATTCTTGATACCGCAACAGCTCTCCAGATTAAAGACGCAATTGATATTCTGGAAGACAAACTCAAAACCCTTCTAAAAG
TTCTGGTAAACCAGGCTGAAGCCCACAAAAATACTGTCTGCTGCGGAAGGACTCACGGGCAGATAGGTGTTCCAACCACT
TACGGTCTTCGTTTTGCAATCTGGGCTTCTGAAATCTCAAGGCATCTTGACCGCCTGTACGAACTGACTCCAAGAGCTAC
TGTGGGGCAGATGACCGGCGCTGTAGGCACTCAGGCAGCTTTTGGAAAATCGGGAATCCTTATTCAGAAACTTACAATGC
AACACCTCGGAATCGGGGCTGTTGATGTCTCAAACCAGATTATCCAGAGGGACAGACATGCCGAGTTTGTTATGTGGATG
GCAAATGTAGTTACAACACTGGACAAGATCGGGATCGAAATCAGGACTCTTCAACGCAGTGAGATCGCCGAGATAGAGGA
AAGTTTCGGGAAAAAGCAGGTAGGTTCATCCACCATGCCTCACAAGCGTAATCCTATCAAGTCCGAACAGATCTGCGGGC
TCGCCCGAATAGTAAGGGCAATGGTTGAGCCTGAACTCCTTAACAACACACTATGGGACGAGAGGGACCTCACGAATTCT
TCAAGTGAACGGATTGTCTTCCCTGAAGCCTGTATACTTACCGATCATATCATTAAACTCGGGATCAGCGTTCTTGAAAA
CCTCAGGTTCTATCCTGAAAATATCCGCAGGAACCTTGATTCCTTAAATGGTCTGAATATGGGCGAGGCTGTTATGATAG
AACTTGCAAAAAGAGGTGTCGGCAGGCAGGAAGCCCACGAACTTGTCCGGACTGCAGCTATGAGAGCTCATGATACAGGG
CAGCATTTCAAACAGGTTCTTCTGGATACTCCTTCCGTTTCAAAATATCTGAGTGCAGGAGATATTGAGAACCTTGTAAA
CCCTGACAAATATATAGGGACAGCAGTGGAACAGGTAGAAGGGCTCGTTATAAAGCTTCGAGAAGCCTATTCACTCTGA

Upstream 100 bases:

>100_bases
AAAACAATCAGGCAGAAAATATCGAACTCAGACTATTCAAAAACCAGGATTAATTTAAAATTTAATGTAGACCCGGGATA
ACTTAATTCTCGATTCCAGT

Downstream 100 bases:

>100_bases
AACTCTTTTCCTTTTTTATTAAATTTCATAAATACAAAAAAAGTAATTTCTTTCGTGATCCTAAAATATTATCGATCGTT
CTGTTTTTATCGACAGGCTT

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MVIDMAIHPIEYRYGTAEMKYVWSQENRLAKILQTEAALARAEADMGLIPAEAAEIIFESIASVKAERVDEIESEIHHDM
MAVVVAISEMCRDDAGKWVHFGATSNDILDTATALQIKDAIDILEDKLKTLLKVLVNQAEAHKNTVCCGRTHGQIGVPTT
YGLRFAIWASEISRHLDRLYELTPRATVGQMTGAVGTQAAFGKSGILIQKLTMQHLGIGAVDVSNQIIQRDRHAEFVMWM
ANVVTTLDKIGIEIRTLQRSEIAEIEESFGKKQVGSSTMPHKRNPIKSEQICGLARIVRAMVEPELLNNTLWDERDLTNS
SSERIVFPEACILTDHIIKLGISVLENLRFYPENIRRNLDSLNGLNMGEAVMIELAKRGVGRQEAHELVRTAAMRAHDTG
QHFKQVLLDTPSVSKYLSAGDIENLVNPDKYIGTAVEQVEGLVIKLREAYSL

Sequences:

>Translated_452_residues
MVIDMAIHPIEYRYGTAEMKYVWSQENRLAKILQTEAALARAEADMGLIPAEAAEIIFESIASVKAERVDEIESEIHHDM
MAVVVAISEMCRDDAGKWVHFGATSNDILDTATALQIKDAIDILEDKLKTLLKVLVNQAEAHKNTVCCGRTHGQIGVPTT
YGLRFAIWASEISRHLDRLYELTPRATVGQMTGAVGTQAAFGKSGILIQKLTMQHLGIGAVDVSNQIIQRDRHAEFVMWM
ANVVTTLDKIGIEIRTLQRSEIAEIEESFGKKQVGSSTMPHKRNPIKSEQICGLARIVRAMVEPELLNNTLWDERDLTNS
SSERIVFPEACILTDHIIKLGISVLENLRFYPENIRRNLDSLNGLNMGEAVMIELAKRGVGRQEAHELVRTAAMRAHDTG
QHFKQVLLDTPSVSKYLSAGDIENLVNPDKYIGTAVEQVEGLVIKLREAYSL
>Mature_452_residues
MVIDMAIHPIEYRYGTAEMKYVWSQENRLAKILQTEAALARAEADMGLIPAEAAEIIFESIASVKAERVDEIESEIHHDM
MAVVVAISEMCRDDAGKWVHFGATSNDILDTATALQIKDAIDILEDKLKTLLKVLVNQAEAHKNTVCCGRTHGQIGVPTT
YGLRFAIWASEISRHLDRLYELTPRATVGQMTGAVGTQAAFGKSGILIQKLTMQHLGIGAVDVSNQIIQRDRHAEFVMWM
ANVVTTLDKIGIEIRTLQRSEIAEIEESFGKKQVGSSTMPHKRNPIKSEQICGLARIVRAMVEPELLNNTLWDERDLTNS
SSERIVFPEACILTDHIIKLGISVLENLRFYPENIRRNLDSLNGLNMGEAVMIELAKRGVGRQEAHELVRTAAMRAHDTG
QHFKQVLLDTPSVSKYLSAGDIENLVNPDKYIGTAVEQVEGLVIKLREAYSL

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=449, Percent_Identity=28.7305122494432, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI183227688, Length=385, Percent_Identity=29.8701298701299, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI31541964, Length=442, Percent_Identity=22.1719457013575, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI68303542, Length=442, Percent_Identity=22.1719457013575, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI68303547, Length=431, Percent_Identity=22.737819025522, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1787376, Length=467, Percent_Identity=26.1241970021413, Blast_Score=82, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17508577, Length=428, Percent_Identity=28.0373831775701, Blast_Score=147, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI32564234, Length=344, Percent_Identity=28.1976744186047, Blast_Score=119, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6323391, Length=463, Percent_Identity=28.7257019438445, Blast_Score=162, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24647570, Length=450, Percent_Identity=28.4444444444444, Blast_Score=151, Evalue=8e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 50306; Mature: 50306

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVIDMAIHPIEYRYGTAEMKYVWSQENRLAKILQTEAALARAEADMGLIPAEAAEIIFES
CEEEEEECCCCEECCCCHHHEECCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
IASVKAERVDEIESEIHHDMMAVVVAISEMCRDDAGKWVHFGATSNDILDTATALQIKDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHH
IDILEDKLKTLLKVLVNQAEAHKNTVCCGRTHGQIGVPTTYGLRFAIWASEISRHLDRLY
HHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHCHHHHHHHHHHHHHHHHHH
ELTPRATVGQMTGAVGTQAAFGKSGILIQKLTMQHLGIGAVDVSNQIIQRDRHAEFVMWM
HCCCCCHHHHHHCCCCCHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
ANVVTTLDKIGIEIRTLQRSEIAEIEESFGKKQVGSSTMPHKRNPIKSEQICGLARIVRA
HHHHHHHHHHCCEEEEHHHHHHHHHHHHHCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
MVEPELLNNTLWDERDLTNSSSERIVFPEACILTDHIIKLGISVLENLRFYPENIRRNLD
HHCHHHHHCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
SLNGLNMGEAVMIELAKRGVGRQEAHELVRTAAMRAHDTGQHFKQVLLDTPSVSKYLSAG
HCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHCC
DIENLVNPDKYIGTAVEQVEGLVIKLREAYSL
CHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVIDMAIHPIEYRYGTAEMKYVWSQENRLAKILQTEAALARAEADMGLIPAEAAEIIFES
CEEEEEECCCCEECCCCHHHEECCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
IASVKAERVDEIESEIHHDMMAVVVAISEMCRDDAGKWVHFGATSNDILDTATALQIKDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHH
IDILEDKLKTLLKVLVNQAEAHKNTVCCGRTHGQIGVPTTYGLRFAIWASEISRHLDRLY
HHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHCHHHHHHHHHHHHHHHHHH
ELTPRATVGQMTGAVGTQAAFGKSGILIQKLTMQHLGIGAVDVSNQIIQRDRHAEFVMWM
HCCCCCHHHHHHCCCCCHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
ANVVTTLDKIGIEIRTLQRSEIAEIEESFGKKQVGSSTMPHKRNPIKSEQICGLARIVRA
HHHHHHHHHHCCEEEEHHHHHHHHHHHHHCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
MVEPELLNNTLWDERDLTNSSSERIVFPEACILTDHIIKLGISVLENLRFYPENIRRNLD
HHCHHHHHCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
SLNGLNMGEAVMIELAKRGVGRQEAHELVRTAAMRAHDTGQHFKQVLLDTPSVSKYLSAG
HCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHCC
DIENLVNPDKYIGTAVEQVEGLVIKLREAYSL
CHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]