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The map label for this gene is fen

Identifier: 21227008

GI number: 21227008

Start: 1071812

End: 1072828

Strand: Reverse

Name: fen

Synonym: MM_0906

Alternate gene names: 21227008

Gene position: 1072828-1071812 (Counterclockwise)

Preceding gene: 21227009

Following gene: 21227005

Centisome position: 26.19

GC content: 46.12

Gene sequence:

>1017_bases
ATGGGTACGGATATAGGAGATCTGCTCCAGAAAAGAAAAATAGAACTTTCTGACCTTTCAAACCGGGTGGTTGCAGTTGA
CGCATTTAACACCCTGCACCAGTTTTTGAGCATTATCCGCCAGAGAGATGGAAGCCCTCTGGTTAATTCACAGGGGAAAG
TAACTTCTCATCTCTCAGGGCTTCTCTATCGCACAGCCAGTCTGGTAGAGGCAGGTATCAAGCCTGTTTTCGTTTTTGAC
GGCAAGCCTCCTGAAATGAAAACAGGAACTCTGAATCGAAGGAAAGAAATCAGGGAATCTTCAAAGGAAAAGTGGGAGAA
TGCAAAAGCAGAGGGCAACCTTGAAGCTGCATATAAGTACGCCCAGGCTTCTTCAAAAGTTGACCAGGACATTATTGAGG
ACTCCAAATATCTCCTCGATATTATGGGCATCCCCTGGATACAGGCTCCATGTGAAGGCGAGGCGCAGGCTGCGCATATG
GTTCTTAAAAAAGATGCTGACTGTGTTGCTTCCCAGGACTACGATTCTTTCCTCTTCGGAGCCCCGACTGTAGTCCGTAA
TCTGGCTGCCACCGGCAAACGCAAGCTCCCCGGGAAAAATGTGTATGTTGATGTTGAACTTGAAATGATTGAGCTTGAGG
AAACCCTGGATTCCCTGGGAATCAACAGAGACCAGCTTATTGATATAGCAATCTGTGTGGGCACGGATTATAATAAAGGA
TTGGAAAAAGTGGGTCCCAAAACAGCCCTCAAGCTAATTAAAAAGCACGGAAACATTCATGCCGTGATCCGGGAAAAAGG
TATGGAGATTGAAGCTCTGGACAGCATAAAGGAACTTTTTACCCATCCTGATGTTACGGATGACTATGAAATCAAATGGG
GAAAACCCGACTCTGAAAAACTCATTAATTTCCTCTGTGACGAGAACGATTTCTCTGAAGACAGGGTGGTAAAAGCCGCA
GATCGCCTTAAAGCGGCTTCAGGGGCAAGACAGCAAACTCTTGACCAGTGGTTCTGA

Upstream 100 bases:

>100_bases
CTGCTGGAGATGATTATTCACCTGACAGCAAAAGGGTTAATGATAATAAACAAAAAAAATGCATCATTGTTTGATATAGA
GTTTTATTCTGAGGGAAAAC

Downstream 100 bases:

>100_bases
AAGGCTTTCAGGATTGCGGCGAAGAAGGTTTAAGGCTGGCAGAATCTGAACCGGGGCATTCGAGGGAAAAATACACTCAT
TCTCACTTACAAGGAGCCGT

Product: flap endonuclease-1

Products: NA

Alternate protein names: FEN-1; Flap structure-specific endonuclease 1

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MGTDIGDLLQKRKIELSDLSNRVVAVDAFNTLHQFLSIIRQRDGSPLVNSQGKVTSHLSGLLYRTASLVEAGIKPVFVFD
GKPPEMKTGTLNRRKEIRESSKEKWENAKAEGNLEAAYKYAQASSKVDQDIIEDSKYLLDIMGIPWIQAPCEGEAQAAHM
VLKKDADCVASQDYDSFLFGAPTVVRNLAATGKRKLPGKNVYVDVELEMIELEETLDSLGINRDQLIDIAICVGTDYNKG
LEKVGPKTALKLIKKHGNIHAVIREKGMEIEALDSIKELFTHPDVTDDYEIKWGKPDSEKLINFLCDENDFSEDRVVKAA
DRLKAASGARQQTLDQWF

Sequences:

>Translated_338_residues
MGTDIGDLLQKRKIELSDLSNRVVAVDAFNTLHQFLSIIRQRDGSPLVNSQGKVTSHLSGLLYRTASLVEAGIKPVFVFD
GKPPEMKTGTLNRRKEIRESSKEKWENAKAEGNLEAAYKYAQASSKVDQDIIEDSKYLLDIMGIPWIQAPCEGEAQAAHM
VLKKDADCVASQDYDSFLFGAPTVVRNLAATGKRKLPGKNVYVDVELEMIELEETLDSLGINRDQLIDIAICVGTDYNKG
LEKVGPKTALKLIKKHGNIHAVIREKGMEIEALDSIKELFTHPDVTDDYEIKWGKPDSEKLINFLCDENDFSEDRVVKAA
DRLKAASGARQQTLDQWF
>Mature_337_residues
GTDIGDLLQKRKIELSDLSNRVVAVDAFNTLHQFLSIIRQRDGSPLVNSQGKVTSHLSGLLYRTASLVEAGIKPVFVFDG
KPPEMKTGTLNRRKEIRESSKEKWENAKAEGNLEAAYKYAQASSKVDQDIIEDSKYLLDIMGIPWIQAPCEGEAQAAHMV
LKKDADCVASQDYDSFLFGAPTVVRNLAATGKRKLPGKNVYVDVELEMIELEETLDSLGINRDQLIDIAICVGTDYNKGL
EKVGPKTALKLIKKHGNIHAVIREKGMEIEALDSIKELFTHPDVTDDYEIKWGKPDSEKLINFLCDENDFSEDRVVKAAD
RLKAASGARQQTLDQWF

Specific function: Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymeras

COG id: COG0258

COG function: function code L; 5'-3' exonuclease (including N-terminal domain of PolI)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily

Homologues:

Organism=Homo sapiens, GI4758356, Length=340, Percent_Identity=36.7647058823529, Blast_Score=221, Evalue=5e-58,
Organism=Homo sapiens, GI194018535, Length=257, Percent_Identity=28.7937743190661, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI194018531, Length=257, Percent_Identity=28.7937743190661, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI51988900, Length=263, Percent_Identity=28.1368821292776, Blast_Score=79, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17510005, Length=333, Percent_Identity=36.3363363363363, Blast_Score=216, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6322736, Length=341, Percent_Identity=37.5366568914956, Blast_Score=208, Evalue=8e-55,
Organism=Saccharomyces cerevisiae, GI6321697, Length=155, Percent_Identity=34.8387096774194, Blast_Score=85, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6324607, Length=251, Percent_Identity=23.9043824701195, Blast_Score=70, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6320469, Length=257, Percent_Identity=24.124513618677, Blast_Score=69, Evalue=9e-13,
Organism=Drosophila melanogaster, GI17647423, Length=352, Percent_Identity=35.2272727272727, Blast_Score=211, Evalue=8e-55,
Organism=Drosophila melanogaster, GI24658219, Length=187, Percent_Identity=26.7379679144385, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI78706854, Length=127, Percent_Identity=33.0708661417323, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI78706850, Length=127, Percent_Identity=33.0708661417323, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI78706852, Length=127, Percent_Identity=33.0708661417323, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FEN_METMA (Q8PYF6)

Other databases:

- EMBL:   AE008384
- RefSeq:   NP_632930.1
- ProteinModelPortal:   Q8PYF6
- SMR:   Q8PYF6
- GeneID:   1479248
- GenomeReviews:   AE008384_GR
- KEGG:   mma:MM_0906
- NMPDR:   fig|192952.1.peg.906
- HOGENOM:   HBG316651
- OMA:   DYDSLLF
- ProtClustDB:   PRK03980
- BioCyc:   MMAZ192952:MM0906-MONOMER
- HAMAP:   MF_00614
- InterPro:   IPR020045
- InterPro:   IPR019973
- InterPro:   IPR008918
- InterPro:   IPR006086
- InterPro:   IPR019974
- InterPro:   IPR006085
- InterPro:   IPR006084
- PANTHER:   PTHR11081
- PRINTS:   PR00853
- SMART:   SM00279
- SMART:   SM00484
- SMART:   SM00485
- TIGRFAMs:   TIGR03674

Pfam domain/function: PF00867 XPG_I; PF00752 XPG_N; SSF47807 5_3_exo_C

EC number: 3.-.-.-

Molecular weight: Translated: 37711; Mature: 37580

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS00841 XPG_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTDIGDLLQKRKIELSDLSNRVVAVDAFNTLHQFLSIIRQRDGSPLVNSQGKVTSHLSG
CCCCHHHHHHHHCCCHHHCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
LLYRTASLVEAGIKPVFVFDGKPPEMKTGTLNRRKEIRESSKEKWENAKAEGNLEAAYKY
HHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
AQASSKVDQDIIEDSKYLLDIMGIPWIQAPCEGEAQAAHMVLKKDADCVASQDYDSFLFG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCHHHCCCCCHHHHC
APTVVRNLAATGKRKLPGKNVYVDVELEMIELEETLDSLGINRDQLIDIAICVGTDYNKG
CHHHHHHHHHCCCCCCCCCCEEEEEEEEHHHHHHHHHHHCCCHHHEEEEEEEECCCCHHH
LEKVGPKTALKLIKKHGNIHAVIREKGMEIEALDSIKELFTHPDVTDDYEIKWGKPDSEK
HHHHCHHHHHHHHHHCCCEEEEEHHCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCHHH
LINFLCDENDFSEDRVVKAADRLKAASGARQQTLDQWF
HHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCC
>Mature Secondary Structure 
GTDIGDLLQKRKIELSDLSNRVVAVDAFNTLHQFLSIIRQRDGSPLVNSQGKVTSHLSG
CCCHHHHHHHHCCCHHHCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
LLYRTASLVEAGIKPVFVFDGKPPEMKTGTLNRRKEIRESSKEKWENAKAEGNLEAAYKY
HHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
AQASSKVDQDIIEDSKYLLDIMGIPWIQAPCEGEAQAAHMVLKKDADCVASQDYDSFLFG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCHHHCCCCCHHHHC
APTVVRNLAATGKRKLPGKNVYVDVELEMIELEETLDSLGINRDQLIDIAICVGTDYNKG
CHHHHHHHHHCCCCCCCCCCEEEEEEEEHHHHHHHHHHHCCCHHHEEEEEEEECCCCHHH
LEKVGPKTALKLIKKHGNIHAVIREKGMEIEALDSIKELFTHPDVTDDYEIKWGKPDSEK
HHHHCHHHHHHHHHHCCCEEEEEHHCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCHHH
LINFLCDENDFSEDRVVKAADRLKAASGARQQTLDQWF
HHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12125824