| Definition | Methanosarcina mazei Go1 chromosome, complete genome. |
|---|---|
| Accession | NC_003901 |
| Length | 4,096,345 |
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The map label for this gene is purH [H]
Identifier: 21227000
GI number: 21227000
Start: 1058082
End: 1059698
Strand: Direct
Name: purH [H]
Synonym: MM_0898
Alternate gene names: 21227000
Gene position: 1058082-1059698 (Clockwise)
Preceding gene: 21226996
Following gene: 21227001
Centisome position: 25.83
GC content: 48.11
Gene sequence:
>1617_bases TTGGTAAAAAGGGCATTGCTCAGCGTCTCAGACAAGACAGGAATTGTAGAATTTGCACGCGGGCTCGAAGCACTTGGCGT GAAAATTATCTCAACAGGCGGGACCGCGAAAATCCTTCGCGATGCAGACATTGAGGTTACTGATGTTTCGGAAGTCACAG GCTACCCGGAGATGATGGGAGGAAGAGTCAAGACCCTCCACCCGAGAATTCATGGAGGACTTTTATGCCTCCGGGAAAGC AAAGAACAGATGGAGGAAGCTGCAAAAGAAGACATCTCCCTTATCGACCTGATAGCCGTAAACCTTTACCCCTTTGAAAT AACCGTTTCCAGAGAGAATGTGGAGCTTGAAGAAGCCATTGAAAATATAGATATCGGAGGCCCTACCCTTCTCCGCTCCG CAGCCAAGAACTACCGCTCGGTGACGGTGTTATCTGACCCCTCGGACTACGGGCGTATCCTTAAGGAGCTGCGCTCAAGC GGGATAATCTCTGATAAAACCCGTGCTGAACTTGCGGTAAAAGCTTTCAGGCACACAGCCGACTATGATGCGGCAATTGA CACATATCTTAGCAGGACCCTTCTTGGGGAAGAAGTTCTCCACCTGAAATTTGCTGACGGTGTAAAGCTTCGCTATGGAG AAAACTGGCACCAGAAAGCATATTTCTATAAAGACTCTGCAATAAAAGGTCCGAGTCTGGCAAAAGCCATCCAGCTTCAT GGAAAAGAACTCTCCTATAACAACTATGTGGATGCGGACAACGCCCTCCAGACTGTCAAAGAACTCGGGAACGCTTCCCC TGCCGTAGCAATAGTAAAACACAATAACCCATGCGGACTTGCTACCGGAGAGTCCCTCCTTCAGGCGCTGCATTCTGCCT GGGACGGGGACCCTATTTCGGCTTATGGGAGTATAATCTGCACAAACGAGGTTTTTGACCTGGAAGCTGCAACTTTTTTG AACGGGAAATTCGTAGAAATTATCCTTGCCCCTGATTTCAAGCCCGATGCACTTGAATACCTGAAAAAGAAAAGCGAAAA TCTGAGGCTCCTGAAACTTCCTGACCTCAGGGAGGGCTTCGGAGCGGAATACACCTACAAATACGTCATAGGCGGCATGC TCAAGCAGAGCCGGGACATAGGGATCTACGAAAAATGGGAGTCTGTTACTGAAGTACCTTATCCTGAAAATAAGCGTGCC CTCTCGGAGTTTTGCCTTAAAGCATGTAAAGCTACAAAGTCCAATGCCGTGATCCTTGCTTATGAATATGAACCAGGGAA TTTCATGGTCCTGGGAATGGGAGCCGGGCAGCCTAACAGGGTAGATTCCATCCGTAAACTGGCAGCCACAAAAGCAGTTG AAAACCTGAAAGTAATCTACGAACGTGAGCAGCCTGCTGTACCCTTTGAAGAATACTGCCAGAAGATTATGTCAGAATGT GTAATGGCTTCAGATGCTTTCTTCCCATTTGATGACAGCGTGGTCCATGCAGCTGAGAATAATATCCGCTATATCGTTTC CCCTGGTGGCTCGATAAGGGACAATGAGGTCATTGCTACTGCAAACAGACTTGGAGTTGCCCTTGTTTTCACAGGAATGC GGCATTTCCTTCACTGA
Upstream 100 bases:
>100_bases CTCTGATTTTTTCAACGAAAAAACCTCTTTCAAATAAAATAATGTGTAAACCATAAGTTTTATAGCTAATAATTTTTAAA CCAAGTATATTGGTGATTTT
Downstream 100 bases:
>100_bases AAAAATAGAGACAAAGAGGACTCAGGTATAACTGAGTCCAGAATAAAACATGAGTACTCCTGCGTACAGGGGCAGGGTAC GCCTAATTTTAGATCTGTCT
Product: IMP cyclohydrolase
Products: NA
Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]
Number of amino acids: Translated: 538; Mature: 538
Protein sequence:
>538_residues MVKRALLSVSDKTGIVEFARGLEALGVKIISTGGTAKILRDADIEVTDVSEVTGYPEMMGGRVKTLHPRIHGGLLCLRES KEQMEEAAKEDISLIDLIAVNLYPFEITVSRENVELEEAIENIDIGGPTLLRSAAKNYRSVTVLSDPSDYGRILKELRSS GIISDKTRAELAVKAFRHTADYDAAIDTYLSRTLLGEEVLHLKFADGVKLRYGENWHQKAYFYKDSAIKGPSLAKAIQLH GKELSYNNYVDADNALQTVKELGNASPAVAIVKHNNPCGLATGESLLQALHSAWDGDPISAYGSIICTNEVFDLEAATFL NGKFVEIILAPDFKPDALEYLKKKSENLRLLKLPDLREGFGAEYTYKYVIGGMLKQSRDIGIYEKWESVTEVPYPENKRA LSEFCLKACKATKSNAVILAYEYEPGNFMVLGMGAGQPNRVDSIRKLAATKAVENLKVIYEREQPAVPFEEYCQKIMSEC VMASDAFFPFDDSVVHAAENNIRYIVSPGGSIRDNEVIATANRLGVALVFTGMRHFLH
Sequences:
>Translated_538_residues MVKRALLSVSDKTGIVEFARGLEALGVKIISTGGTAKILRDADIEVTDVSEVTGYPEMMGGRVKTLHPRIHGGLLCLRES KEQMEEAAKEDISLIDLIAVNLYPFEITVSRENVELEEAIENIDIGGPTLLRSAAKNYRSVTVLSDPSDYGRILKELRSS GIISDKTRAELAVKAFRHTADYDAAIDTYLSRTLLGEEVLHLKFADGVKLRYGENWHQKAYFYKDSAIKGPSLAKAIQLH GKELSYNNYVDADNALQTVKELGNASPAVAIVKHNNPCGLATGESLLQALHSAWDGDPISAYGSIICTNEVFDLEAATFL NGKFVEIILAPDFKPDALEYLKKKSENLRLLKLPDLREGFGAEYTYKYVIGGMLKQSRDIGIYEKWESVTEVPYPENKRA LSEFCLKACKATKSNAVILAYEYEPGNFMVLGMGAGQPNRVDSIRKLAATKAVENLKVIYEREQPAVPFEEYCQKIMSEC VMASDAFFPFDDSVVHAAENNIRYIVSPGGSIRDNEVIATANRLGVALVFTGMRHFLH >Mature_538_residues MVKRALLSVSDKTGIVEFARGLEALGVKIISTGGTAKILRDADIEVTDVSEVTGYPEMMGGRVKTLHPRIHGGLLCLRES KEQMEEAAKEDISLIDLIAVNLYPFEITVSRENVELEEAIENIDIGGPTLLRSAAKNYRSVTVLSDPSDYGRILKELRSS GIISDKTRAELAVKAFRHTADYDAAIDTYLSRTLLGEEVLHLKFADGVKLRYGENWHQKAYFYKDSAIKGPSLAKAIQLH GKELSYNNYVDADNALQTVKELGNASPAVAIVKHNNPCGLATGESLLQALHSAWDGDPISAYGSIICTNEVFDLEAATFL NGKFVEIILAPDFKPDALEYLKKKSENLRLLKLPDLREGFGAEYTYKYVIGGMLKQSRDIGIYEKWESVTEVPYPENKRA LSEFCLKACKATKSNAVILAYEYEPGNFMVLGMGAGQPNRVDSIRKLAATKAVENLKVIYEREQPAVPFEEYCQKIMSEC VMASDAFFPFDDSVVHAAENNIRYIVSPGGSIRDNEVIATANRLGVALVFTGMRHFLH
Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]
COG id: COG0138
COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the purH family [H]
Homologues:
Organism=Homo sapiens, GI20127454, Length=621, Percent_Identity=37.037037037037, Blast_Score=293, Evalue=3e-79, Organism=Escherichia coli, GI1790439, Length=553, Percent_Identity=44.8462929475588, Blast_Score=423, Evalue=1e-119, Organism=Caenorhabditis elegans, GI71985564, Length=616, Percent_Identity=34.5779220779221, Blast_Score=303, Evalue=2e-82, Organism=Caenorhabditis elegans, GI71985574, Length=328, Percent_Identity=30.4878048780488, Blast_Score=95, Evalue=8e-20, Organism=Saccharomyces cerevisiae, GI6323768, Length=626, Percent_Identity=33.7060702875399, Blast_Score=280, Evalue=5e-76, Organism=Saccharomyces cerevisiae, GI6323056, Length=617, Percent_Identity=33.7115072933549, Blast_Score=270, Evalue=4e-73, Organism=Drosophila melanogaster, GI24649832, Length=613, Percent_Identity=35.2365415986949, Blast_Score=288, Evalue=5e-78,
Paralogues:
None
Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002695 - InterPro: IPR013982 - InterPro: IPR016193 - InterPro: IPR011607 [H]
Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]
EC number: =2.1.2.3; =3.5.4.10 [H]
Molecular weight: Translated: 59495; Mature: 59495
Theoretical pI: Translated: 5.56; Mature: 5.56
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.3 %Cys (Translated Protein) 1.9 %Met (Translated Protein) 3.2 %Cys+Met (Translated Protein) 1.3 %Cys (Mature Protein) 1.9 %Met (Mature Protein) 3.2 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MVKRALLSVSDKTGIVEFARGLEALGVKIISTGGTAKILRDADIEVTDVSEVTGYPEMMG CCCHHHCCCCCCCCHHHHHCCHHHHCEEEEECCCCEEEEECCCCEEEEHHHHCCCHHHHC GRVKTLHPRIHGGLLCLRESKEQMEEAAKEDISLIDLIAVNLYPFEITVSRENVELEEAI CEEEEECCCCCCCEEEEHHHHHHHHHHHHHCCCEEEEEEEEEEEEEEEEECCCCCHHHHH ENIDIGGPTLLRSAAKNYRSVTVLSDPSDYGRILKELRSSGIISDKTRAELAVKAFRHTA HHCCCCCHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCC DYDAAIDTYLSRTLLGEEVLHLKFADGVKLRYGENWHQKAYFYKDSAIKGPSLAKAIQLH CCHHHHHHHHHHHHHHHHHEEEEECCCEEEEECCCCHHCEEEEECCCCCCCHHHHHHHHC GKELSYNNYVDADNALQTVKELGNASPAVAIVKHNNPCGLATGESLLQALHSAWDGDPIS CCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCHH AYGSIICTNEVFDLEAATFLNGKFVEIILAPDFKPDALEYLKKKSENLRLLKLPDLREGF HCCCEEEECCEECCCHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCHHCC GAEYTYKYVIGGMLKQSRDIGIYEKWESVTEVPYPENKRALSEFCLKACKATKSNAVILA CCCEEHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEE YEYEPGNFMVLGMGAGQPNRVDSIRKLAATKAVENLKVIYEREQPAVPFEEYCQKIMSEC EEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHH VMASDAFFPFDDSVVHAAENNIRYIVSPGGSIRDNEVIATANRLGVALVFTGMRHFLH HHHCCCCCCCCCCEEEECCCCEEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCC >Mature Secondary Structure MVKRALLSVSDKTGIVEFARGLEALGVKIISTGGTAKILRDADIEVTDVSEVTGYPEMMG CCCHHHCCCCCCCCHHHHHCCHHHHCEEEEECCCCEEEEECCCCEEEEHHHHCCCHHHHC GRVKTLHPRIHGGLLCLRESKEQMEEAAKEDISLIDLIAVNLYPFEITVSRENVELEEAI CEEEEECCCCCCCEEEEHHHHHHHHHHHHHCCCEEEEEEEEEEEEEEEEECCCCCHHHHH ENIDIGGPTLLRSAAKNYRSVTVLSDPSDYGRILKELRSSGIISDKTRAELAVKAFRHTA HHCCCCCHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCC DYDAAIDTYLSRTLLGEEVLHLKFADGVKLRYGENWHQKAYFYKDSAIKGPSLAKAIQLH CCHHHHHHHHHHHHHHHHHEEEEECCCEEEEECCCCHHCEEEEECCCCCCCHHHHHHHHC GKELSYNNYVDADNALQTVKELGNASPAVAIVKHNNPCGLATGESLLQALHSAWDGDPIS CCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCHH AYGSIICTNEVFDLEAATFLNGKFVEIILAPDFKPDALEYLKKKSENLRLLKLPDLREGF HCCCEEEECCEECCCHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCHHCC GAEYTYKYVIGGMLKQSRDIGIYEKWESVTEVPYPENKRALSEFCLKACKATKSNAVILA CCCEEHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEE YEYEPGNFMVLGMGAGQPNRVDSIRKLAATKAVENLKVIYEREQPAVPFEEYCQKIMSEC EEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHH VMASDAFFPFDDSVVHAAENNIRYIVSPGGSIRDNEVIATANRLGVALVFTGMRHFLH HHHCCCCCCCCCCEEEECCCCEEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA