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The map label for this gene is lpdA [C]

Identifier: 21226998

GI number: 21226998

Start: 1056333

End: 1056650

Strand: Reverse

Name: lpdA [C]

Synonym: MM_0896

Alternate gene names: 21226998

Gene position: 1056650-1056333 (Counterclockwise)

Preceding gene: 21226999

Following gene: 21226997

Centisome position: 25.79

GC content: 43.71

Gene sequence:

>318_bases
GTGCAGTATTTACCCATCCCTGTGCTTGCTTCCGTTGGAATAAGTGAGGAAAAAGAAAGTGATAAATACAGGGTTATTTT
CCGTGACCGCAGTACATGGAGCACTACCAGGAGGGCAGGGATGGAATTTGCAGCTTCAAAGATAATTCTTGACGAAGAAA
ATGACCATATAATGGGAGCCCACATCCTGGGACCCAATGCCGAAGAAGTTATCAATATCTTTGCCATGGCAATGCGTCTC
GGGCTCAGGACTTCGGATATAAGGAAAGTTGTATTTACCTACCCGACAATATGTTCCGATATTCGATATATGCTGTAA

Upstream 100 bases:

>100_bases
GGAATTCAGCTTACTCCGGTAGCAACTCTTCAGGGAGAAGTTGCAGCTGCTAATATTTTTGACAGAAACGGGGCTGAAGC
CGATTATACAGGAATTCCAA

Downstream 100 bases:

>100_bases
TTCCGGTAGCTTTAAAGACTGCCTTTTTCTTTTATCTGGTCCGAAAAACTTAGAAATAAAAAACGACCAATACTTTAACA
GATACATTTCAAAAAGCTGT

Product: hypothetical protein

Products: lipoamide; NADH; acetyl-CoA; CO2; Proton; NH3; 5,10-methylene-THF; succinyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 105; Mature: 105

Protein sequence:

>105_residues
MQYLPIPVLASVGISEEKESDKYRVIFRDRSTWSTTRRAGMEFAASKIILDEENDHIMGAHILGPNAEEVINIFAMAMRL
GLRTSDIRKVVFTYPTICSDIRYML

Sequences:

>Translated_105_residues
MQYLPIPVLASVGISEEKESDKYRVIFRDRSTWSTTRRAGMEFAASKIILDEENDHIMGAHILGPNAEEVINIFAMAMRL
GLRTSDIRKVVFTYPTICSDIRYML
>Mature_105_residues
MQYLPIPVLASVGISEEKESDKYRVIFRDRSTWSTTRRAGMEFAASKIILDEENDHIMGAHILGPNAEEVINIFAMAMRL
GLRTSDIRKVVFTYPTICSDIRYML

Specific function: Lipoamide Dehydrogenase Is A Component Of The Glycine Cleavage System As Well As Of The Alpha-Ketoacid Dehydrogenase Complexes. [C]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR015723
- InterPro:   IPR004099 [H]

Pfam domain/function: PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: 1.8.1.4 [C]

Molecular weight: Translated: 12013; Mature: 12013

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQYLPIPVLASVGISEEKESDKYRVIFRDRSTWSTTRRAGMEFAASKIILDEENDHIMGA
CCCCCCHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHEEEEECCCCEEEEE
HILGPNAEEVINIFAMAMRLGLRTSDIRKVVFTYPTICSDIRYML
EEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHCC
>Mature Secondary Structure
MQYLPIPVLASVGISEEKESDKYRVIFRDRSTWSTTRRAGMEFAASKIILDEENDHIMGA
CCCCCCHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHEEEEECCCCEEEEE
HILGPNAEEVINIFAMAMRLGLRTSDIRKVVFTYPTICSDIRYML
EEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADH [C]

Metal ions: NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {lipoamide}} 1.1 {NAD+}} [C]

Substrates: Dihydrolipoamide; NAD+; pyruvate; Coenzyme A; L-glycine; THF; 2-oxoglutarate [C]

Specific reaction: Dihydrolipoamide + NAD+ = lipoamide + NADH pyruvate + Coenzyme A + NAD = acetyl-CoA + CO2 + NADH + Proton NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF 2-oxoglutarate + Coenzyme A + NAD = succinyl-CoA + CO2 + NADH + Proton [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; p-Aminophenyl dichloroarsine [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3037534 [H]