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The map label for this gene is nth [H]

Identifier: 21226997

GI number: 21226997

Start: 1055481

End: 1056185

Strand: Reverse

Name: nth [H]

Synonym: MM_0895

Alternate gene names: 21226997

Gene position: 1056185-1055481 (Counterclockwise)

Preceding gene: 21226998

Following gene: 21226994

Centisome position: 25.78

GC content: 43.97

Gene sequence:

>705_bases
ATGCCTGAAAAAAAACCCAGAAAATCGACTTCAAAAGAAGTCCTGATTGAATATGATATTCCTGACAACAGGCATAACTT
TGACCGCATCTGGGACCTTCTAAAAGAGGAATACACGGATGCAAAACCATCTCTTAATTACAGCAATCCTTTGGAACTCC
TTGTGGCAACCGTTCTTTCTGCCCAGTCGACGGACGTTCAGATCAATAAGGTGACGGAAAATCTGTTCAAAAAGTACAGG
ACCGCCTGGGATTACGCAAGCGCAGATATCAGAGAACTGGAAGCGGATATTTATTCTACAGGGTTTTACAAAAGCAAAGC
AAAAAATATCAAAGCGGCTGCACAACTGATTATCGAAAATTATGGGGGAGAAGTCCCGCAAACAATGGAAGAACTCGTCA
CCCTTCCAGGAGTGGGGAGAAAGACCGCAAATATAGTGCTTGCCAGGGCTTTTGGGATAATAGAGGGTATTGCCGTGGAT
ACTCATGTGAAAAGAGTCTCAGGAAGGCTGGGGCTTACCAGGAATTCGGACCCGGTAAAAATAGAACAGGACCTTATATC
CCTTGCCAGGAAAGAAGACCTTGATTCGATTTCCATGACCCTGATCTATCACGGGAGAAAAGTTTGCCAGGCAAGAAAAC
CGAGATGCTCGATCTGTGTTGTAAAAGAACTGTGCCCTTCCAGCATTATTTTTATTGGAGAGTAA

Upstream 100 bases:

>100_bases
ACTTAGAAATAAAAAACGACCAATACTTTAACAGATACATTTCAAAAAGCTGTTTTATAGTTATAATCTGAAATTTGAAC
CTCTTAACGGAGTTCTTTTA

Downstream 100 bases:

>100_bases
ACCGGGGTTTCAGGGTACAAAAATAGTTTTACAGTTTTATTAAGAGGAATCTGGAATGCTCATTCCAGAGCATGCACTGT
GGTAATTTAAGAGTAGCCGT

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 234; Mature: 233

Protein sequence:

>234_residues
MPEKKPRKSTSKEVLIEYDIPDNRHNFDRIWDLLKEEYTDAKPSLNYSNPLELLVATVLSAQSTDVQINKVTENLFKKYR
TAWDYASADIRELEADIYSTGFYKSKAKNIKAAAQLIIENYGGEVPQTMEELVTLPGVGRKTANIVLARAFGIIEGIAVD
THVKRVSGRLGLTRNSDPVKIEQDLISLARKEDLDSISMTLIYHGRKVCQARKPRCSICVVKELCPSSIIFIGE

Sequences:

>Translated_234_residues
MPEKKPRKSTSKEVLIEYDIPDNRHNFDRIWDLLKEEYTDAKPSLNYSNPLELLVATVLSAQSTDVQINKVTENLFKKYR
TAWDYASADIRELEADIYSTGFYKSKAKNIKAAAQLIIENYGGEVPQTMEELVTLPGVGRKTANIVLARAFGIIEGIAVD
THVKRVSGRLGLTRNSDPVKIEQDLISLARKEDLDSISMTLIYHGRKVCQARKPRCSICVVKELCPSSIIFIGE
>Mature_233_residues
PEKKPRKSTSKEVLIEYDIPDNRHNFDRIWDLLKEEYTDAKPSLNYSNPLELLVATVLSAQSTDVQINKVTENLFKKYRT
AWDYASADIRELEADIYSTGFYKSKAKNIKAAAQLIIENYGGEVPQTMEELVTLPGVGRKTANIVLARAFGIIEGIAVDT
HVKRVSGRLGLTRNSDPVKIEQDLISLARKEDLDSISMTLIYHGRKVCQARKPRCSICVVKELCPSSIIFIGE

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=179, Percent_Identity=34.0782122905028, Blast_Score=114, Evalue=5e-26,
Organism=Homo sapiens, GI6912520, Length=217, Percent_Identity=29.9539170506912, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI115298648, Length=217, Percent_Identity=29.9539170506912, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI190358497, Length=217, Percent_Identity=29.9539170506912, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI115298654, Length=217, Percent_Identity=29.9539170506912, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI115298652, Length=217, Percent_Identity=29.9539170506912, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI115298650, Length=217, Percent_Identity=29.9539170506912, Blast_Score=83, Evalue=2e-16,
Organism=Escherichia coli, GI1787920, Length=192, Percent_Identity=40.625, Blast_Score=146, Evalue=1e-36,
Organism=Escherichia coli, GI1789331, Length=182, Percent_Identity=28.021978021978, Blast_Score=81, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17554540, Length=179, Percent_Identity=35.195530726257, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6324530, Length=190, Percent_Identity=27.8947368421053, Blast_Score=90, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6319304, Length=76, Percent_Identity=43.421052631579, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI45550361, Length=179, Percent_Identity=34.0782122905028, Blast_Score=108, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 26349; Mature: 26217

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEKKPRKSTSKEVLIEYDIPDNRHNFDRIWDLLKEEYTDAKPSLNYSNPLELLVATVLS
CCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
AQSTDVQINKVTENLFKKYRTAWDYASADIRELEADIYSTGFYKSKAKNIKAAAQLIIEN
CCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
YGGEVPQTMEELVTLPGVGRKTANIVLARAFGIIEGIAVDTHVKRVSGRLGLTRNSDPVK
CCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
IEQDLISLARKEDLDSISMTLIYHGRKVCQARKPRCSICVVKELCPSSIIFIGE
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEHHHCCCCCEEEECC
>Mature Secondary Structure 
PEKKPRKSTSKEVLIEYDIPDNRHNFDRIWDLLKEEYTDAKPSLNYSNPLELLVATVLS
CCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
AQSTDVQINKVTENLFKKYRTAWDYASADIRELEADIYSTGFYKSKAKNIKAAAQLIIEN
CCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
YGGEVPQTMEELVTLPGVGRKTANIVLARAFGIIEGIAVDTHVKRVSGRLGLTRNSDPVK
CCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
IEQDLISLARKEDLDSISMTLIYHGRKVCQARKPRCSICVVKELCPSSIIFIGE
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]