Click here to switch to the map view.

The map label for this gene is purA

Identifier: 21226903

GI number: 21226903

Start: 954914

End: 956053

Strand: Direct

Name: purA

Synonym: MM_0801

Alternate gene names: 21226903

Gene position: 954914-956053 (Clockwise)

Preceding gene: 21226902

Following gene: 21226904

Centisome position: 23.31

GC content: 48.07

Gene sequence:

>1140_bases
ATGTTTACGATAATCACAGGCGCGCAGTTCGGTGACGAAGGAAAGGGTAAGATTGTCGACCTTCTTGCAAAAGACTATGA
CGTTGTTGCCCGCTTCCAGGGAGGGAATAACGCGGGCCATACAGTAAAGGTAGGAGATGAGGTCTACAAACTGCACCTGA
TTCCTTCAGGCATCCTGCTTGATGCCAGGGTCCTTATCGGGCCTGGGGTTGTCCTTAACCCTGAGGTTTTAGCTGAAGAA
ATCGAAATGTTTGAAAAGCACGGAGTAAAGGTCAATTCGGAAAAGCTGGGAGTGGACGCCAAGACAAGCATTATTATGCC
CTACCACATAGAGCTTGACGGCCTCAGAGAAGCTTCAAGGGAAACAAAAATAGGGACCACAAAACGCGGAATCGGTTATG
CTTATATTGACAAGGTGGCAAGAGACGAGATCCGCATGGCTGAACTTGTGGATAAAGAGCGCTTCCTTGCAAGGCTTGAA
GAACTCGCCCCCCAGAAAGAAAAAGAAATTGAAGCAATGGGCGGTGACCCCAGGATCGTAAGAGACCCTGAACTTATAAA
GAGGTACCTGGAACTGGGAGAGCAGTTTGCTGCTTATATTACTGATGTTTCCAGGGAAATCAACCAGGCTCTTGACGAAG
GAAAAAATGTAATGGCTGAAGCTGCCCAGGGCACACATCTCGACGTTATTCACGGAACCCAGAAGTTCGTAACTTCTTCT
TCTACAATTGCAGGTTCTGCATGTGCAAACCTCGGGGTAGGTCCAACAAGAGTGGACAATGTCATTGCCATAGTAAAAGC
CTATATAACCAGAGTAGGGGAAGGCCCGCTTCCGACAGAGCTCATTGGAGAACTTGGAGAAAGAATACAGAAAGCCGGAG
GAGAATTCGGGACAACTACAGGCAGAGGAAGAAGATGCGGCTGGTTTGACCTCCCACTCCTCAAGAAGGCTATTGCTCTC
AATGGATACACGGAAATTTCCCTTACGAAACTGGACGTACTTACCGGGCTTGACCCTCTAAGATCTGCACAGGTTATGAG
TATAAAGGGAAAAAACTGGACTATCCTCCGGAACTTACGGAAGACCTCCAGGCATGCAGCCCTGTGTATGAAGACTTGCC
AGGATGGAAAGAAGAACTGA

Upstream 100 bases:

>100_bases
CGAAAGAAACTGAGAAGAAATTTCAGGTTGAAAATACGCTGAGAAGAAGGTAAAACAAACCGGACAAAACCTTATTAAGG
TTAAGCCCGATTCAGGGCTT

Downstream 100 bases:

>100_bases
CAGAGGTCAAAGCCTACAATGAGCTCCCGGAAAATGCCAGAAATTATGTTGAGAAGCTGGAAGAGCTCATGAAAGTCCCT
GTCAATTATATTTCCGTAGG

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MFTIITGAQFGDEGKGKIVDLLAKDYDVVARFQGGNNAGHTVKVGDEVYKLHLIPSGILLDARVLIGPGVVLNPEVLAEE
IEMFEKHGVKVNSEKLGVDAKTSIIMPYHIELDGLREASRETKIGTTKRGIGYAYIDKVARDEIRMAELVDKERFLARLE
ELAPQKEKEIEAMGGDPRIVRDPELIKRYLELGEQFAAYITDVSREINQALDEGKNVMAEAAQGTHLDVIHGTQKFVTSS
STIAGSACANLGVGPTRVDNVIAIVKAYITRVGEGPLPTELIGELGERIQKAGGEFGTTTGRGRRCGWFDLPLLKKAIAL
NGYTEISLTKLDVLTGLDPLRSAQVMSIKGKNWTILRNLRKTSRHAALCMKTCQDGKKN

Sequences:

>Translated_379_residues
MFTIITGAQFGDEGKGKIVDLLAKDYDVVARFQGGNNAGHTVKVGDEVYKLHLIPSGILLDARVLIGPGVVLNPEVLAEE
IEMFEKHGVKVNSEKLGVDAKTSIIMPYHIELDGLREASRETKIGTTKRGIGYAYIDKVARDEIRMAELVDKERFLARLE
ELAPQKEKEIEAMGGDPRIVRDPELIKRYLELGEQFAAYITDVSREINQALDEGKNVMAEAAQGTHLDVIHGTQKFVTSS
STIAGSACANLGVGPTRVDNVIAIVKAYITRVGEGPLPTELIGELGERIQKAGGEFGTTTGRGRRCGWFDLPLLKKAIAL
NGYTEISLTKLDVLTGLDPLRSAQVMSIKGKNWTILRNLRKTSRHAALCMKTCQDGKKN
>Mature_379_residues
MFTIITGAQFGDEGKGKIVDLLAKDYDVVARFQGGNNAGHTVKVGDEVYKLHLIPSGILLDARVLIGPGVVLNPEVLAEE
IEMFEKHGVKVNSEKLGVDAKTSIIMPYHIELDGLREASRETKIGTTKRGIGYAYIDKVARDEIRMAELVDKERFLARLE
ELAPQKEKEIEAMGGDPRIVRDPELIKRYLELGEQFAAYITDVSREINQALDEGKNVMAEAAQGTHLDVIHGTQKFVTSS
STIAGSACANLGVGPTRVDNVIAIVKAYITRVGEGPLPTELIGELGERIQKAGGEFGTTTGRGRRCGWFDLPLLKKAIAL
NGYTEISLTKLDVLTGLDPLRSAQVMSIKGKNWTILRNLRKTSRHAALCMKTCQDGKKN

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=357, Percent_Identity=41.7366946778711, Blast_Score=253, Evalue=1e-67,
Organism=Homo sapiens, GI34577063, Length=341, Percent_Identity=43.108504398827, Blast_Score=240, Evalue=2e-63,
Organism=Homo sapiens, GI40316944, Length=327, Percent_Identity=39.4495412844037, Blast_Score=209, Evalue=4e-54,
Organism=Escherichia coli, GI1790620, Length=340, Percent_Identity=46.4705882352941, Blast_Score=270, Evalue=1e-73,
Organism=Caenorhabditis elegans, GI25146553, Length=360, Percent_Identity=40.5555555555556, Blast_Score=244, Evalue=6e-65,
Organism=Caenorhabditis elegans, GI25146555, Length=360, Percent_Identity=40.5555555555556, Blast_Score=244, Evalue=7e-65,
Organism=Saccharomyces cerevisiae, GI6324109, Length=363, Percent_Identity=39.9449035812672, Blast_Score=253, Evalue=5e-68,
Organism=Drosophila melanogaster, GI21356033, Length=344, Percent_Identity=40.406976744186, Blast_Score=248, Evalue=5e-66,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_METMA (Q8PYQ9)

Other databases:

- EMBL:   AE008384
- RefSeq:   NP_632825.1
- ProteinModelPortal:   Q8PYQ9
- SMR:   Q8PYQ9
- GeneID:   1479143
- GenomeReviews:   AE008384_GR
- KEGG:   mma:MM_0801
- NMPDR:   fig|192952.1.peg.801
- HOGENOM:   HBG658237
- OMA:   ARDEIRM
- BioCyc:   MMAZ192952:MM0801-MONOMER
- BRENDA:   6.3.4.4
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 41453; Mature: 41453

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 12-12 ACT_SITE 40-40 BINDING 127-127 BINDING 141-141 BINDING 223-223 BINDING 238-238 BINDING 302-302 BINDING 304-304

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTIITGAQFGDEGKGKIVDLLAKDYDVVARFQGGNNAGHTVKVGDEVYKLHLIPSGILL
CEEEEECCCCCCCCCCHHHHHHHCCCEEEEEECCCCCCCCEEEECCEEEEEEECCCCCEE
DARVLIGPGVVLNPEVLAEEIEMFEKHGVKVNSEKLGVDAKTSIIMPYHIELDGLREASR
ECCEEECCCEEECHHHHHHHHHHHHHCCCEECCHHCCCCCCCEEEEEEEEEEHHHHHHHH
ETKIGTTKRGIGYAYIDKVARDEIRMAELVDKERFLARLEELAPQKEKEIEAMGGDPRIV
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCEE
RDPELIKRYLELGEQFAAYITDVSREINQALDEGKNVMAEAAQGTHLDVIHGTQKFVTSS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHCCC
STIAGSACANLGVGPTRVDNVIAIVKAYITRVGEGPLPTELIGELGERIQKAGGEFGTTT
CCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
GRGRRCGWFDLPLLKKAIALNGYTEISLTKLDVLTGLDPLRSAQVMSIKGKNWTILRNLR
CCCCCCCCCCHHHHHHHHHCCCCCEEEEEHHHHHHCCCCCCCCEEEEECCCCCHHHHHHH
KTSRHAALCMKTCQDGKKN
HHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MFTIITGAQFGDEGKGKIVDLLAKDYDVVARFQGGNNAGHTVKVGDEVYKLHLIPSGILL
CEEEEECCCCCCCCCCHHHHHHHCCCEEEEEECCCCCCCCEEEECCEEEEEEECCCCCEE
DARVLIGPGVVLNPEVLAEEIEMFEKHGVKVNSEKLGVDAKTSIIMPYHIELDGLREASR
ECCEEECCCEEECHHHHHHHHHHHHHCCCEECCHHCCCCCCCEEEEEEEEEEHHHHHHHH
ETKIGTTKRGIGYAYIDKVARDEIRMAELVDKERFLARLEELAPQKEKEIEAMGGDPRIV
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCEE
RDPELIKRYLELGEQFAAYITDVSREINQALDEGKNVMAEAAQGTHLDVIHGTQKFVTSS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHCCC
STIAGSACANLGVGPTRVDNVIAIVKAYITRVGEGPLPTELIGELGERIQKAGGEFGTTT
CCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
GRGRRCGWFDLPLLKKAIALNGYTEISLTKLDVLTGLDPLRSAQVMSIKGKNWTILRNLR
CCCCCCCCCCHHHHHHHHHCCCCCEEEEEHHHHHHCCCCCCCCEEEEECCCCCHHHHHHH
KTSRHAALCMKTCQDGKKN
HHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12125824