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The map label for this gene is rfaG [C]

Identifier: 212225007

GI number: 212225007

Start: 1735946

End: 1737121

Strand: Reverse

Name: rfaG [C]

Synonym: TON_1855

Alternate gene names: 212225007

Gene position: 1737121-1735946 (Counterclockwise)

Preceding gene: 212225008

Following gene: 212225006

Centisome position: 94.02

GC content: 35.8

Gene sequence:

>1176_bases
ATGGATACTATACAAGTGGTACAAGTATACCATCATGACCCATGTGATAAAGGAAAGGGTGGTGTTGTTAGATATATTAA
TAATATTTTAAAGTATATGCCAAGGAAAGGCATTAAAGTGACATTATTCGGAGTTGATACCGGTAAATGCCAAGAAAATG
AATTAGATACTCTAAAGATATCATTTGTGCCGATAATTAAATCGTCCGCCACATGGTGGAGGTATTTGATCAAATTAACT
TTTAGATTGCCTTTCATGAGAATACCAAAAGAAAGCGTAATCCATGTGCATCGCATAGAATATGTTTTACCATTTATAAT
CTCAAAACGAAAGAATCCTGTGGTACTCACACTACACGGAGAGAGATTAGCAACAGCAAAAGTGAAATATCAAAAAGCAT
TCCCTTTAATTGAAGCAATTTATAGAATACTAGAATATTTCATTTTCAAAAGAGCCAATGTCATTGTAGCAGTATCTAAC
AAAGTGAAAGAGTCCTTTGAAAATGTTCATAAAAACATATCGGATAAAATCATTGTACTCCCAGTAGGAGTGGATATGGA
TGAATTCAAACCACTAGATAAGGATTACCTAAGACAAAAATACGACATAGGCGAGGAAACCAAAGTTCTACTTTTTGCGG
GAGTGCTAGAAGAAAGGAAAAACATTGGCTTGTTAATAAGAGCATTTAGATACGTCCAAGAGAGATATCCAGACTCTATA
TTACTTATCGTGGGAGACGGCCCATTGAGGTCCTCCTTAGAAAAGTTAGTTGAAGAGTTAAAAATTAAGTCGAAAGTGTG
GTTTTTAGGTGAAGTTTCCCGCGAGCAGTTACCAGAGAGTTATAATCTTGCGGACGTATTCGCTCTCCCATCTTTATCTG
AGGGAAGTCCTACTGTAGTAAGAGAAGCACTAGCGTGTGGTATTCCAGTAGTTTCTACTGATGTAGGGGATGTTAAGGAA
ATAATTACGGACCCTCTCCTAGGCACAGTCGTTAACACATATACTGACGAAAGAGTTTTTGCAGAAGCATTAATAGAGAC
TATTGAAAACATTGACAAAAATAACGAAGAAATAAAGAAGAGATGCCGAGATATAGCAAGAAAGTATAGTTTTGAAAATG
TTGTTGAAAAATATGTTGAAATTTACCACAAACTTAGAAGTGGAGAGCAAAGGTGA

Upstream 100 bases:

>100_bases
ACTAGATATTTATGTGTTTGATAACTTCAAGAGCTTGTTCAAATTAAAAGAAGAGCCTATTATATTACGTGATTTCAAAG
GAGCTTAGGGGGATAAACAT

Downstream 100 bases:

>100_bases
GCAAGAATGGGGAGGCATAAAAAACAATTGCTATCGTATTTACTATTGATCTTTAGTTTGCTGTTACCAATTCTCTTATA
TAGTGGATTAGTTAATCCGT

Product: glycosyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MDTIQVVQVYHHDPCDKGKGGVVRYINNILKYMPRKGIKVTLFGVDTGKCQENELDTLKISFVPIIKSSATWWRYLIKLT
FRLPFMRIPKESVIHVHRIEYVLPFIISKRKNPVVLTLHGERLATAKVKYQKAFPLIEAIYRILEYFIFKRANVIVAVSN
KVKESFENVHKNISDKIIVLPVGVDMDEFKPLDKDYLRQKYDIGEETKVLLFAGVLEERKNIGLLIRAFRYVQERYPDSI
LLIVGDGPLRSSLEKLVEELKIKSKVWFLGEVSREQLPESYNLADVFALPSLSEGSPTVVREALACGIPVVSTDVGDVKE
IITDPLLGTVVNTYTDERVFAEALIETIENIDKNNEEIKKRCRDIARKYSFENVVEKYVEIYHKLRSGEQR

Sequences:

>Translated_391_residues
MDTIQVVQVYHHDPCDKGKGGVVRYINNILKYMPRKGIKVTLFGVDTGKCQENELDTLKISFVPIIKSSATWWRYLIKLT
FRLPFMRIPKESVIHVHRIEYVLPFIISKRKNPVVLTLHGERLATAKVKYQKAFPLIEAIYRILEYFIFKRANVIVAVSN
KVKESFENVHKNISDKIIVLPVGVDMDEFKPLDKDYLRQKYDIGEETKVLLFAGVLEERKNIGLLIRAFRYVQERYPDSI
LLIVGDGPLRSSLEKLVEELKIKSKVWFLGEVSREQLPESYNLADVFALPSLSEGSPTVVREALACGIPVVSTDVGDVKE
IITDPLLGTVVNTYTDERVFAEALIETIENIDKNNEEIKKRCRDIARKYSFENVVEKYVEIYHKLRSGEQR
>Mature_391_residues
MDTIQVVQVYHHDPCDKGKGGVVRYINNILKYMPRKGIKVTLFGVDTGKCQENELDTLKISFVPIIKSSATWWRYLIKLT
FRLPFMRIPKESVIHVHRIEYVLPFIISKRKNPVVLTLHGERLATAKVKYQKAFPLIEAIYRILEYFIFKRANVIVAVSN
KVKESFENVHKNISDKIIVLPVGVDMDEFKPLDKDYLRQKYDIGEETKVLLFAGVLEERKNIGLLIRAFRYVQERYPDSI
LLIVGDGPLRSSLEKLVEELKIKSKVWFLGEVSREQLPESYNLADVFALPSLSEGSPTVVREALACGIPVVSTDVGDVKE
IITDPLLGTVVNTYTDERVFAEALIETIENIDKNNEEIKKRCRDIARKYSFENVVEKYVEIYHKLRSGEQR

Specific function: Involved In The Addition Of The First Glucose Residue To The Lipopolysaccharide Core. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Homo sapiens, GI11863130, Length=236, Percent_Identity=25.4237288135593, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1790061, Length=217, Percent_Identity=28.110599078341, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17532709, Length=230, Percent_Identity=26.0869565217391, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI9755344, Length=241, Percent_Identity=24.896265560166, Blast_Score=80, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR013534 [H]

Pfam domain/function: PF08323 Glyco_transf_5; PF00534 Glycos_transf_1 [H]

EC number: NA

Molecular weight: Translated: 45050; Mature: 45050

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTIQVVQVYHHDPCDKGKGGVVRYINNILKYMPRKGIKVTLFGVDTGKCQENELDTLKI
CCHHHEEHEECCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCEEEE
SFVPIIKSSATWWRYLIKLTFRLPFMRIPKESVIHVHRIEYVLPFIISKRKNPVVLTLHG
EEEEEECCCHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECC
ERLATAKVKYQKAFPLIEAIYRILEYFIFKRANVIVAVSNKVKESFENVHKNISDKIIVL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHCCCCCCEEEE
PVGVDMDEFKPLDKDYLRQKYDIGEETKVLLFAGVLEERKNIGLLIRAFRYVQERYPDSI
EECCCHHHCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCEE
LLIVGDGPLRSSLEKLVEELKIKSKVWFLGEVSREQLPESYNLADVFALPSLSEGSPTVV
EEEECCCHHHHHHHHHHHHHHHHHHEEEEECCCHHHCCCCCCCHHHHCCCCCCCCCHHHH
REALACGIPVVSTDVGDVKEIITDPLLGTVVNTYTDERVFAEALIETIENIDKNNEEIKK
HHHHHCCCCEEECCHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHH
RCRDIARKYSFENVVEKYVEIYHKLRSGEQR
HHHHHHHHCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDTIQVVQVYHHDPCDKGKGGVVRYINNILKYMPRKGIKVTLFGVDTGKCQENELDTLKI
CCHHHEEHEECCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCEEEE
SFVPIIKSSATWWRYLIKLTFRLPFMRIPKESVIHVHRIEYVLPFIISKRKNPVVLTLHG
EEEEEECCCHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECC
ERLATAKVKYQKAFPLIEAIYRILEYFIFKRANVIVAVSNKVKESFENVHKNISDKIIVL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHCCCCCCEEEE
PVGVDMDEFKPLDKDYLRQKYDIGEETKVLLFAGVLEERKNIGLLIRAFRYVQERYPDSI
EECCCHHHCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCEE
LLIVGDGPLRSSLEKLVEELKIKSKVWFLGEVSREQLPESYNLADVFALPSLSEGSPTVV
EEEECCCHHHHHHHHHHHHHHHHHHEEEEECCCHHHCCCCCCCHHHHCCCCCCCCCHHHH
REALACGIPVVSTDVGDVKEIITDPLLGTVVNTYTDERVFAEALIETIENIDKNNEEIKK
HHHHHCCCCEEECCHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHH
RCRDIARKYSFENVVEKYVEIYHKLRSGEQR
HHHHHHHHCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Glycosyltransferases; Hexosyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]