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The map label for this gene is priB [H]

Identifier: 212224929

GI number: 212224929

Start: 1643624

End: 1644829

Strand: Reverse

Name: priB [H]

Synonym: TON_1778

Alternate gene names: 212224929

Gene position: 1644829-1643624 (Counterclockwise)

Preceding gene: 212224933

Following gene: 212224928

Centisome position: 89.02

GC content: 51.24

Gene sequence:

>1206_bases
ATGCTGGATCCCTTTGGAAGCGAAGCCAAGAGGCTTGTCAAGGAAGAATTCGGTGGAATAAACGAATTGCTTTCAATAAT
TCCTTCATACATTGGAATAGAGCATGCACTGGAGAGGGTCTCGTGGATAAAATCCGGGGAGATTCCAGAAGACATCCTTG
AGCTAAGCGACATCCAGGATTTACTGACATTCTATGCCCTTCTTGGTGCTCTTGCCTTTTCGCCGTACGGAATCGAGATG
GAGCTCGTGAGAGAGGCCAATATTAAGATATACTCCGAGCGGATAAGGAGAGCCAGCAAAATAGAAGGAACGGCAATACC
TTTGAAGATTGTTGAAAGGGATGAGATTCCCAATAGGGACAGAACAATACTCGAAAGAACCCACCACACCGAACTTCCTC
CAAGCGAGAGAGAAAAGCTGAGACTGAGATACAAGACCGGGCTCAAAAAGTTCCTCGAGCTTTGGGACGGGAGCCTCAAA
GAAGTCTACATCAGAAAAGGCCACGCTTACCTGACAGGGGAACAGGCGCTGAAGCTGTGGGAGGAGGCCTTTGAGAAGAA
CTTCGAAAAAGCGGTAAATCTGCTCTACGAGATAAGAGACGAGCTGTCCACCTACTACCTCCAGCTCTATGAGAAGCTGA
GCGAGATAGCCAGGGAGTACTTTAAGGAAAGACTCGAGAGGATGGGTTCTGCAGAGGCCCAACCGCTTCGCTTTGAGCTG
TTCCCCCCATGCGTTAAAATCGCCCTTGGCGGCGTCCCCGCTGGTTTGAGGAACTACGCGATAACCGTCCTCCTGACGAG
CTTCCTGAGCTATGCGAGGCTCTGCCCCAACCCACCGCGGAGAGACGTCAGGGTCAGGGACTGCGTTCAGGATTTAAGCG
TAATCGAGAAGGAGATATTTCCCATTATCATAGAGGCAGGAAACAGGTGCAGCCCACCGCTATTCGAAGATCAGCCTCAC
GAGATCAAAAACATCTGGTACCACCTCGGCTTCGGCTTAACAGACAAACCAACGCTCGAGGACAGCGGGAACTCTACATG
GTACTTCCCTCCCAACTGCTCCAAAATAAGGGCCAATGCTCCCCAGCTCTGCAAGCCCGACAGACACTGCAGGAACATCA
AGAACCCACTGACGTACTACCTGAGACGGCTCTACCTGGAGAACAGAAAGAAAGGGGAGCGCGAAGAGGGTGGTGGAAGT
GGCTGA

Upstream 100 bases:

>100_bases
TAATTGCAAACATGCAATGTTCTTGTCAGCAGACAAAACTTTCTTGGGAAAGGAAACCGTTTTATGTTCTAACCAACACC
CATTGATGAGAGGTGAGGTC

Downstream 100 bases:

>100_bases
GCTATTTAGGGAGGTCACCAAAGAAGAGAGAGTCCTCTACTACAAAGCCGAATGGAACGCAAAGAAGCTACCAAGGTTCC
TAGTTGAGAAGCTGGAAAAC

Product: DNA primase large subunit

Products: NA

Alternate protein names: DNA primase 46 kDa subunit; p46 [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MLDPFGSEAKRLVKEEFGGINELLSIIPSYIGIEHALERVSWIKSGEIPEDILELSDIQDLLTFYALLGALAFSPYGIEM
ELVREANIKIYSERIRRASKIEGTAIPLKIVERDEIPNRDRTILERTHHTELPPSEREKLRLRYKTGLKKFLELWDGSLK
EVYIRKGHAYLTGEQALKLWEEAFEKNFEKAVNLLYEIRDELSTYYLQLYEKLSEIAREYFKERLERMGSAEAQPLRFEL
FPPCVKIALGGVPAGLRNYAITVLLTSFLSYARLCPNPPRRDVRVRDCVQDLSVIEKEIFPIIIEAGNRCSPPLFEDQPH
EIKNIWYHLGFGLTDKPTLEDSGNSTWYFPPNCSKIRANAPQLCKPDRHCRNIKNPLTYYLRRLYLENRKKGEREEGGGS
G

Sequences:

>Translated_401_residues
MLDPFGSEAKRLVKEEFGGINELLSIIPSYIGIEHALERVSWIKSGEIPEDILELSDIQDLLTFYALLGALAFSPYGIEM
ELVREANIKIYSERIRRASKIEGTAIPLKIVERDEIPNRDRTILERTHHTELPPSEREKLRLRYKTGLKKFLELWDGSLK
EVYIRKGHAYLTGEQALKLWEEAFEKNFEKAVNLLYEIRDELSTYYLQLYEKLSEIAREYFKERLERMGSAEAQPLRFEL
FPPCVKIALGGVPAGLRNYAITVLLTSFLSYARLCPNPPRRDVRVRDCVQDLSVIEKEIFPIIIEAGNRCSPPLFEDQPH
EIKNIWYHLGFGLTDKPTLEDSGNSTWYFPPNCSKIRANAPQLCKPDRHCRNIKNPLTYYLRRLYLENRKKGEREEGGGS
G
>Mature_401_residues
MLDPFGSEAKRLVKEEFGGINELLSIIPSYIGIEHALERVSWIKSGEIPEDILELSDIQDLLTFYALLGALAFSPYGIEM
ELVREANIKIYSERIRRASKIEGTAIPLKIVERDEIPNRDRTILERTHHTELPPSEREKLRLRYKTGLKKFLELWDGSLK
EVYIRKGHAYLTGEQALKLWEEAFEKNFEKAVNLLYEIRDELSTYYLQLYEKLSEIAREYFKERLERMGSAEAQPLRFEL
FPPCVKIALGGVPAGLRNYAITVLLTSFLSYARLCPNPPRRDVRVRDCVQDLSVIEKEIFPIIIEAGNRCSPPLFEDQPH
EIKNIWYHLGFGLTDKPTLEDSGNSTWYFPPNCSKIRANAPQLCKPDRHCRNIKNPLTYYLRRLYLENRKKGEREEGGGS
G

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG2219

COG function: function code L; Eukaryotic-type DNA primase, large subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eukaryotic-type primase large subunit family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007238 [H]

Pfam domain/function: PF04104 DNA_primase_lrg [H]

EC number: NA

Molecular weight: Translated: 46411; Mature: 46411

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDPFGSEAKRLVKEEFGGINELLSIIPSYIGIEHALERVSWIKSGEIPEDILELSDIQD
CCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
LLTFYALLGALAFSPYGIEMELVREANIKIYSERIRRASKIEGTAIPLKIVERDEIPNRD
HHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCH
RTILERTHHTELPPSEREKLRLRYKTGLKKFLELWDGSLKEVYIRKGHAYLTGEQALKLW
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEECHHHHHHHH
EEAFEKNFEKAVNLLYEIRDELSTYYLQLYEKLSEIAREYFKERLERMGSAEAQPLRFEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEC
FPPCVKIALGGVPAGLRNYAITVLLTSFLSYARLCPNPPRRDVRVRDCVQDLSVIEKEIF
CCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
PIIIEAGNRCSPPLFEDQPHEIKNIWYHLGFGLTDKPTLEDSGNSTWYFPPNCSKIRANA
HHHEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCHHCCCC
PQLCKPDRHCRNIKNPLTYYLRRLYLENRKKGEREEGGGSG
CCCCCCHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MLDPFGSEAKRLVKEEFGGINELLSIIPSYIGIEHALERVSWIKSGEIPEDILELSDIQD
CCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
LLTFYALLGALAFSPYGIEMELVREANIKIYSERIRRASKIEGTAIPLKIVERDEIPNRD
HHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCH
RTILERTHHTELPPSEREKLRLRYKTGLKKFLELWDGSLKEVYIRKGHAYLTGEQALKLW
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEECHHHHHHHH
EEAFEKNFEKAVNLLYEIRDELSTYYLQLYEKLSEIAREYFKERLERMGSAEAQPLRFEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEC
FPPCVKIALGGVPAGLRNYAITVLLTSFLSYARLCPNPPRRDVRVRDCVQDLSVIEKEIF
CCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
PIIIEAGNRCSPPLFEDQPHEIKNIWYHLGFGLTDKPTLEDSGNSTWYFPPNCSKIRANA
HHHEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCHHCCCC
PQLCKPDRHCRNIKNPLTYYLRRLYLENRKKGEREEGGGSG
CCCCCCHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11584001 [H]