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The map label for this gene is priA

Identifier: 212224928

GI number: 212224928

Start: 1642591

End: 1643631

Strand: Reverse

Name: priA

Synonym: TON_1777

Alternate gene names: 212224928

Gene position: 1643631-1642591 (Counterclockwise)

Preceding gene: 212224929

Following gene: 212224927

Centisome position: 88.96

GC content: 52.45

Gene sequence:

>1041_bases
GTGGCTGAGCTATTTAGGGAGGTCACCAAAGAAGAGAGAGTCCTCTACTACAAAGCCGAATGGAACGCAAAGAAGCTACC
AAGGTTCCTAGTTGAGAAGCTGGAAAACAGGGAGTTCGGCTTTGACCACACAGGGGAAGGGCCAAGCGATAGGAAAAACG
TCTTCATGGACGTCAGAGATCTGGAGGACTACATCAAGGCCACGGCCCCCTATGCGATTTACTCGAGCGTTGCACTGTAC
GAGGATCCAAAAGGGATGAGCGGCTGGCTCGGTGCGGAGCTGGTGTTCGACATAGACGCCAAGGACTTGCCCCTGAGAAG
GTGCGGACACATTCACGAGCACGGAAAGGTGTGTCCAATTTGTCTGGGTGACGCGAAGGAGCTCGCAAGAGACACCCTGG
TGATTTTAAAGGAGGACTTCGGTTTTGAGGATGTTCACGTGGTCTATTCCGGGAGGGGTTATCACATAAGAGTTCTCGAT
GACTGGGCCATCCAGCTTGACTCGAAATCAAGAGAGAAGATTTTAGCCTATATTTCGGCGGCCGAAGAGGTAACTTTCGA
AGACATCCAGAGCAGGAAGATAATGCTCTCCTCGGGCTACTTCAGGGTCTTTCGCCTTCGCTTCGGCTACTTCATCGCCC
GCGCCAACGAGAATCACCTGCTTAACATCGGGCTGAGAAAAGGGCAGGTGAAGAAGATACTCGAGAGCCGCGATGAGATA
TACGAGGGCTTCGTCAGAAAGGGTCTTTTAACGGCCTTTCCACAGGGGATAGGATATAAAACACTGGCGAGGCTGTTCGC
CCTCTCCAGCACTTTCTCAAAGGCCTACTTTGACGGGAGAGTCACGGTTGACGTCAAGAGAATCCTCCGCGTTCCATCCA
GCCTGCACTCAAAAGTTGGACTGGTAGCGACATACATCGGCTCAGACGAGAGAAAGCTCGAGAAGTTCAATCCCTTCCGC
GATGCGGTTCCAAAGTTCAGGGAGGAAGAAGTCAAAGAGGCCTACGAAGAGTGGCTCGAGCTTCACGGGGATGAGCTGTG
A

Upstream 100 bases:

>100_bases
GACAGACACTGCAGGAACATCAAGAACCCACTGACGTACTACCTGAGACGGCTCTACCTGGAGAACAGAAAGAAAGGGGA
GCGCGAAGAGGGTGGTGGAA

Downstream 100 bases:

>100_bases
ACGGAAGACTGAAAATAATTGTCTCAATGCTCATATGGGGAAGCGTGGGCATCTTCGCGCGTTTTTCCAACCTCTCAGGA
CTAGGGGTAGCCTTCTTCAG

Product: DNA primase small subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 346; Mature: 345

Protein sequence:

>346_residues
MAELFREVTKEERVLYYKAEWNAKKLPRFLVEKLENREFGFDHTGEGPSDRKNVFMDVRDLEDYIKATAPYAIYSSVALY
EDPKGMSGWLGAELVFDIDAKDLPLRRCGHIHEHGKVCPICLGDAKELARDTLVILKEDFGFEDVHVVYSGRGYHIRVLD
DWAIQLDSKSREKILAYISAAEEVTFEDIQSRKIMLSSGYFRVFRLRFGYFIARANENHLLNIGLRKGQVKKILESRDEI
YEGFVRKGLLTAFPQGIGYKTLARLFALSSTFSKAYFDGRVTVDVKRILRVPSSLHSKVGLVATYIGSDERKLEKFNPFR
DAVPKFREEEVKEAYEEWLELHGDEL

Sequences:

>Translated_346_residues
MAELFREVTKEERVLYYKAEWNAKKLPRFLVEKLENREFGFDHTGEGPSDRKNVFMDVRDLEDYIKATAPYAIYSSVALY
EDPKGMSGWLGAELVFDIDAKDLPLRRCGHIHEHGKVCPICLGDAKELARDTLVILKEDFGFEDVHVVYSGRGYHIRVLD
DWAIQLDSKSREKILAYISAAEEVTFEDIQSRKIMLSSGYFRVFRLRFGYFIARANENHLLNIGLRKGQVKKILESRDEI
YEGFVRKGLLTAFPQGIGYKTLARLFALSSTFSKAYFDGRVTVDVKRILRVPSSLHSKVGLVATYIGSDERKLEKFNPFR
DAVPKFREEEVKEAYEEWLELHGDEL
>Mature_345_residues
AELFREVTKEERVLYYKAEWNAKKLPRFLVEKLENREFGFDHTGEGPSDRKNVFMDVRDLEDYIKATAPYAIYSSVALYE
DPKGMSGWLGAELVFDIDAKDLPLRRCGHIHEHGKVCPICLGDAKELARDTLVILKEDFGFEDVHVVYSGRGYHIRVLDD
WAIQLDSKSREKILAYISAAEEVTFEDIQSRKIMLSSGYFRVFRLRFGYFIARANENHLLNIGLRKGQVKKILESRDEIY
EGFVRKGLLTAFPQGIGYKTLARLFALSSTFSKAYFDGRVTVDVKRILRVPSSLHSKVGLVATYIGSDERKLEKFNPFRD
AVPKFREEEVKEAYEEWLELHGDEL

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis

COG id: COG1467

COG function: function code L; Eukaryotic-type DNA primase, catalytic (small) subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eukaryotic-type primase small subunit family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PRIS_THEON (B6YV39)

Other databases:

- EMBL:   CP000855
- RefSeq:   YP_002308164.1
- GeneID:   7017446
- GenomeReviews:   CP000855_GR
- KEGG:   ton:TON_1777
- HOGENOM:   HBG551858
- OMA:   REFGFDH
- ProtClustDB:   PRK00419
- HAMAP:   MF_00700
- InterPro:   IPR002755
- InterPro:   IPR014052
- PANTHER:   PTHR10536
- TIGRFAMs:   TIGR00335

Pfam domain/function: PF01896 DNA_primase_S

EC number: NA

Molecular weight: Translated: 39973; Mature: 39842

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: ACT_SITE 97-97 ACT_SITE 99-99

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAELFREVTKEERVLYYKAEWNAKKLPRFLVEKLENREFGFDHTGEGPSDRKNVFMDVRD
CHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHEEEHHH
LEDYIKATAPYAIYSSVALYEDPKGMSGWLGAELVFDIDAKDLPLRRCGHIHEHGKVCPI
HHHHHHHCCCHHHHHCEEEECCCCCCCCCCCCEEEEECCCCCCCHHHHCCHHHCCCEEEE
CLGDAKELARDTLVILKEDFGFEDVHVVYSGRGYHIRVLDDWAIQLDSKSREKILAYISA
EECCHHHHHHHHEEEEECCCCCCEEEEEEECCCEEEEEEECEEEEECCCHHHHHHHHHHH
AEEVTFEDIQSRKIMLSSGYFRVFRLRFGYFIARANENHLLNIGLRKGQVKKILESRDEI
HHHHHHHHHHCCEEEEECCHHHHHHHHHHHEEEECCCCCEEEEECCCHHHHHHHHHHHHH
YEGFVRKGLLTAFPQGIGYKTLARLFALSSTFSKAYFDGRVTVDVKRILRVPSSLHSKVG
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHCCHHHHHHHC
LVATYIGSDERKLEKFNPFRDAVPKFREEEVKEAYEEWLELHGDEL
EEEEECCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AELFREVTKEERVLYYKAEWNAKKLPRFLVEKLENREFGFDHTGEGPSDRKNVFMDVRD
HHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHEEEHHH
LEDYIKATAPYAIYSSVALYEDPKGMSGWLGAELVFDIDAKDLPLRRCGHIHEHGKVCPI
HHHHHHHCCCHHHHHCEEEECCCCCCCCCCCCEEEEECCCCCCCHHHHCCHHHCCCEEEE
CLGDAKELARDTLVILKEDFGFEDVHVVYSGRGYHIRVLDDWAIQLDSKSREKILAYISA
EECCHHHHHHHHEEEEECCCCCCEEEEEEECCCEEEEEEECEEEEECCCHHHHHHHHHHH
AEEVTFEDIQSRKIMLSSGYFRVFRLRFGYFIARANENHLLNIGLRKGQVKKILESRDEI
HHHHHHHHHHCCEEEEECCHHHHHHHHHHHEEEECCCCCEEEEECCCHHHHHHHHHHHHH
YEGFVRKGLLTAFPQGIGYKTLARLFALSSTFSKAYFDGRVTVDVKRILRVPSSLHSKVG
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHCCHHHHHHHC
LVATYIGSDERKLEKFNPFRDAVPKFREEEVKEAYEEWLELHGDEL
EEEEECCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA