| Definition | Thermococcus onnurineus NA1, complete genome. |
|---|---|
| Accession | NC_011529 |
| Length | 1,847,607 |
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The map label for this gene is amyA [H]
Identifier: 212224811
GI number: 212224811
Start: 1524547
End: 1525923
Strand: Reverse
Name: amyA [H]
Synonym: TON_1660
Alternate gene names: 212224811
Gene position: 1525923-1524547 (Counterclockwise)
Preceding gene: 212224812
Following gene: 212224801
Centisome position: 82.59
GC content: 55.56
Gene sequence:
>1377_bases ATGGCCAGAAAAGCAGCCGTTGCAGTTTTGATGGCCATTCTGATTTCCATGAGCCTGTTTGCAGTTCCAGCCAGGGCGGA AACACTGGAAAACGGCGGAGTCATAATGCAGGCCTTCTACTGGGATGTGCCAATGGGTGGAATTTGGTGGGATACAATAA GAGCCAAGGTGCCGGAGTGGTCACAAGCGGGCATAGCCGCAATATGGCTACCTCCGGCGAGTAAAGGCATGAGCGGCAAC TACTCCATGGGCTACGATCCATACGACTACTTCGACCTCGGCGAGTATGACCAGAAGGGAACGGTAGAAACGAGGTTCGG CTCAAAGGACGAGCTCATATCCCTAATCAACACGGCACACGCCTACAACATGAAGGTGGTAGCGGACATAGTCATAAACC ACAGGGCGGGCGGCGATTTAGAGTGGAACCCCTTCGTCAACGACTACACCTGGACGGACTTTTCTGGTGTTGCCTCAGGC AAATACACCGCCTGCTACCTCGACTTCCATCCCAATGACGTCAGCTACGCCGATGAAGGCACCTTCGGAGGTTTCCCCGA CATAGACCACGACAAAGAGTGGAACCAGTACTGGCTGTGGAAGAGCAACGAGAGCTACGCTGCTTACCTCAGGAGCATTG GAATTGACGCCTGGCGCTTTGACTACGTCAAGGGCTACCCGGCGTGGGTGGTTAACTCGTGGCTGAGCTACTGGGGAGGC TGGGCCGTTGGAGAATACTGGGACACTAACGTCGACGCCCTTCTCAACTGGGCTTACTCCAGTGGTGCCAAAGTCTTTGA CTTCCCGCTCTACTACAAGATGGACGAGGCCTTCGATAACAACAACATCCCCGCCCTCGTCGATGCCCTCAGATACGGCC AGACCGTAGTTAGCCGCGACCCGTTCAAGGCAGTGACCTTCGTCGCCAACCACGACACGGACATAATCTGGAACAAGTAT CCAGCTTATGCGTTCATCCTCACCTACGAGGGTCAGCCCATGATATTCTACCGCGACTACGAGGAGTGGCTCAACAAGGA CAAGCTGATAAACCTCATCTGGATACACGACAACCTCGCGGGGGGGAGCACCGACATAGTCTACTACGACAGCGACGAGC TGATATTCGTCAGAAGCGGCTACGGAAGCAAACCGGGACTGATAACCTATATCAACCTCGGCTCAAGCAAAGCCGGAAGG TGGGTCTACGTTCCGAAGTTTGCCGGGTCGTGCATACACGAGTACACCGGCAACCTCGGCGGCTGGATTGACAAGTGGGT GGACTCAAGCGGCTGGGTCTACCTTGAAGCCCCGGCCCACGACCCGGCCAACGGCCAGTACGGCTACTCCGTCTGGAGCT ACTGTGGTGTTGGCTGA
Upstream 100 bases:
>100_bases CCAGTGGATGAACGATTAGGTTCTCCAATGCACATTGGCGGCAAAGTTTAAATATTTCACCATCGGTGATATATTGTGAG CAGCTTACCCGGGTGGTAAC
Downstream 100 bases:
>100_bases ACTCTGTTTTCTTTTCCTATTTAAAAAGACGAAGAAGATTAGAACTCCACCACCCATTTAACCGCGTGCGGCACCTTTTT GGCAACCTCCAAAGCGGTGA
Product: cytoplasmic alpha-amylase
Products: NA
Alternate protein names: 1,4-alpha-D-glucan glucanohydrolase [H]
Number of amino acids: Translated: 458; Mature: 457
Protein sequence:
>458_residues MARKAAVAVLMAILISMSLFAVPARAETLENGGVIMQAFYWDVPMGGIWWDTIRAKVPEWSQAGIAAIWLPPASKGMSGN YSMGYDPYDYFDLGEYDQKGTVETRFGSKDELISLINTAHAYNMKVVADIVINHRAGGDLEWNPFVNDYTWTDFSGVASG KYTACYLDFHPNDVSYADEGTFGGFPDIDHDKEWNQYWLWKSNESYAAYLRSIGIDAWRFDYVKGYPAWVVNSWLSYWGG WAVGEYWDTNVDALLNWAYSSGAKVFDFPLYYKMDEAFDNNNIPALVDALRYGQTVVSRDPFKAVTFVANHDTDIIWNKY PAYAFILTYEGQPMIFYRDYEEWLNKDKLINLIWIHDNLAGGSTDIVYYDSDELIFVRSGYGSKPGLITYINLGSSKAGR WVYVPKFAGSCIHEYTGNLGGWIDKWVDSSGWVYLEAPAHDPANGQYGYSVWSYCGVG
Sequences:
>Translated_458_residues MARKAAVAVLMAILISMSLFAVPARAETLENGGVIMQAFYWDVPMGGIWWDTIRAKVPEWSQAGIAAIWLPPASKGMSGN YSMGYDPYDYFDLGEYDQKGTVETRFGSKDELISLINTAHAYNMKVVADIVINHRAGGDLEWNPFVNDYTWTDFSGVASG KYTACYLDFHPNDVSYADEGTFGGFPDIDHDKEWNQYWLWKSNESYAAYLRSIGIDAWRFDYVKGYPAWVVNSWLSYWGG WAVGEYWDTNVDALLNWAYSSGAKVFDFPLYYKMDEAFDNNNIPALVDALRYGQTVVSRDPFKAVTFVANHDTDIIWNKY PAYAFILTYEGQPMIFYRDYEEWLNKDKLINLIWIHDNLAGGSTDIVYYDSDELIFVRSGYGSKPGLITYINLGSSKAGR WVYVPKFAGSCIHEYTGNLGGWIDKWVDSSGWVYLEAPAHDPANGQYGYSVWSYCGVG >Mature_457_residues ARKAAVAVLMAILISMSLFAVPARAETLENGGVIMQAFYWDVPMGGIWWDTIRAKVPEWSQAGIAAIWLPPASKGMSGNY SMGYDPYDYFDLGEYDQKGTVETRFGSKDELISLINTAHAYNMKVVADIVINHRAGGDLEWNPFVNDYTWTDFSGVASGK YTACYLDFHPNDVSYADEGTFGGFPDIDHDKEWNQYWLWKSNESYAAYLRSIGIDAWRFDYVKGYPAWVVNSWLSYWGGW AVGEYWDTNVDALLNWAYSSGAKVFDFPLYYKMDEAFDNNNIPALVDALRYGQTVVSRDPFKAVTFVANHDTDIIWNKYP AYAFILTYEGQPMIFYRDYEEWLNKDKLINLIWIHDNLAGGSTDIVYYDSDELIFVRSGYGSKPGLITYINLGSSKAGRW VYVPKFAGSCIHEYTGNLGGWIDKWVDSSGWVYLEAPAHDPANGQYGYSVWSYCGVG
Specific function: Unknown
COG id: COG0366
COG function: function code G; Glycosidases
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the glycosyl hydrolase 13 family [H]
Homologues:
Organism=Escherichia coli, GI1788236, Length=444, Percent_Identity=30.1801801801802, Blast_Score=154, Evalue=1e-38,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR013776 - InterPro: IPR013780 - InterPro: IPR006047 - InterPro: IPR006589 - InterPro: IPR017853 - InterPro: IPR013781 [H]
Pfam domain/function: PF00128 Alpha-amylase [H]
EC number: =3.2.1.1 [H]
Molecular weight: Translated: 51948; Mature: 51817
Theoretical pI: Translated: 4.40; Mature: 4.40
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.7 %Cys (Translated Protein) 2.2 %Met (Translated Protein) 2.8 %Cys+Met (Translated Protein) 0.7 %Cys (Mature Protein) 2.0 %Met (Mature Protein) 2.6 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MARKAAVAVLMAILISMSLFAVPARAETLENGGVIMQAFYWDVPMGGIWWDTIRAKVPEW CCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEEEEEEECCCCCCEEHHHHHCCCCCC SQAGIAAIWLPPASKGMSGNYSMGYDPYDYFDLGEYDQKGTVETRFGSKDELISLINTAH CCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHH AYNMKVVADIVINHRAGGDLEWNPFVNDYTWTDFSGVASGKYTACYLDFHPNDVSYADEG HCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCC TFGGFPDIDHDKEWNQYWLWKSNESYAAYLRSIGIDAWRFDYVKGYPAWVVNSWLSYWGG CCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCEEEEHHHCCCHHHHHHHHHHHHCC WAVGEYWDTNVDALLNWAYSSGAKVFDFPLYYKMDEAFDNNNIPALVDALRYGQTVVSRD CCCCCCCCCCHHHHEEEHHCCCCEEEEECEEEEEHHHCCCCCCHHHHHHHHHCCHHHCCC PFKAVTFVANHDTDIIWNKYPAYAFILTYEGQPMIFYRDYEEWLNKDKLINLIWIHDNLA CCEEEEEEECCCCCEEEECCCCEEEEEEECCCEEEEEECHHHHCCCCCEEEEEEEECCCC GGSTDIVYYDSDELIFVRSGYGSKPGLITYINLGSSKAGRWVYVPKFAGSCIHEYTGNLG CCCEEEEEECCCCEEEEECCCCCCCCEEEEEECCCCCCCCEEEECCHHHHHHHHHCCCCC GWIDKWVDSSGWVYLEAPAHDPANGQYGYSVWSYCGVG HHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHCCCC >Mature Secondary Structure ARKAAVAVLMAILISMSLFAVPARAETLENGGVIMQAFYWDVPMGGIWWDTIRAKVPEW CHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEEEEEEECCCCCCEEHHHHHCCCCCC SQAGIAAIWLPPASKGMSGNYSMGYDPYDYFDLGEYDQKGTVETRFGSKDELISLINTAH CCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHH AYNMKVVADIVINHRAGGDLEWNPFVNDYTWTDFSGVASGKYTACYLDFHPNDVSYADEG HCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCC TFGGFPDIDHDKEWNQYWLWKSNESYAAYLRSIGIDAWRFDYVKGYPAWVVNSWLSYWGG CCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCEEEEHHHCCCHHHHHHHHHHHHCC WAVGEYWDTNVDALLNWAYSSGAKVFDFPLYYKMDEAFDNNNIPALVDALRYGQTVVSRD CCCCCCCCCCHHHHEEEHHCCCCEEEEECEEEEEHHHCCCCCCHHHHHHHHHCCHHHCCC PFKAVTFVANHDTDIIWNKYPAYAFILTYEGQPMIFYRDYEEWLNKDKLINLIWIHDNLA CCEEEEEEECCCCCEEEECCCCEEEEEEECCCEEEEEECHHHHCCCCCEEEEEEEECCCC GGSTDIVYYDSDELIFVRSGYGSKPGLITYINLGSSKAGRWVYVPKFAGSCIHEYTGNLG CCCEEEEEECCCCEEEEECCCCCCCCEEEEEECCCCCCCCEEEECCHHHHHHHHHCCCCC GWIDKWVDSSGWVYLEAPAHDPANGQYGYSVWSYCGVG HHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 1400215; 11677609; 1527488; 8371104 [H]