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The map label for this gene is speE

Identifier: 212224473

GI number: 212224473

Start: 1200519

End: 1201367

Strand: Reverse

Name: speE

Synonym: TON_1322

Alternate gene names: 212224473

Gene position: 1201367-1200519 (Counterclockwise)

Preceding gene: 212224474

Following gene: 212224472

Centisome position: 65.02

GC content: 53.36

Gene sequence:

>849_bases
ATGGGATTTAACGAGCAGGAGAATGCATTCATCGAGTGGTATCCAAGGGGCTACGGAGTGGGCTTCAGGGTTAAGGAGAG
GCTCTTTGAGACCCAGACTAAGTATCAGAGGCTTGAGCTCTACGAAACCGAAGGTTTCGGCAAACTCCTTGTCCTTGACG
GGACGGTTCAGCTCGTCGAGATGGGTGAGGAAAGCTACCACGAGCCCCTCGTCCACCCCGTCATGCTCGCTCACCCTAAT
CCGAGAAGGGTGCTCATCATAGGTGGCGGCGACGGAGGAACGCTGAGGGAAGTGCTGAGGCACAAAACTGTAGAAAAGGC
CATCATGGTCGAGATAGACGAGATGGTCATAGAGGTCTCCAGGATCTACATGAACGTCGCGAGGGGCGCCTTCGAGGATC
CGAGGGCGGAATTGATAGTCAGCGACGGCGTTGAGTACCTCAAAAACACGGACGAGAAGTTTGACGTCATCATCGTTGAC
TCAACGGACCCTGTTGGACCGGCAAAGATGCTCTTCAGCGAGGGATTCTTCAGAAACGCATACGAGAAGCTCAACGATAA
CGGTCTGTATATCACCCAGGCGGGCAGCGTGTACCTATTCACCAACGAGCTGCTCGACGCCTACAGGGATATGGGGAAGG
TCTTCGACGAAGTCCACTACTTCAGTTTCCCTGTCATAGGCTACGCATCTCCATGGAGCTTCCTCGTCGGAGTCAAGGGA
GATATTAACTTCAGAAAGGTTGACCTTCAGCGCGCTAAGGAGCTTAAGCTCTACTACTACGACCCTGAGAGGCACGAAAC
CCTCTTTCAGATGCCAAAGTACGTGAGGGAGCTTCTGGAGAAGGTCTGA

Upstream 100 bases:

>100_bases
GGACAAGCCGGCTGCGGCCATAGCCGCTGTCGTAATGTTCCCCTACTGAGGGTTTAGTTTTTAAGCCCTTTCCCTTCTTC
AGCTTTTAGAGGTGGAACTT

Downstream 100 bases:

>100_bases
CCCTTCTCCTTACTTCAATACCTTTTTGGAGGTGATTAACCCGTGCTCAAGGCAGCCTTCAAAAACACCAGAATCCTCGA
TGGAAGAGCGTTCATTGGCA

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MGFNEQENAFIEWYPRGYGVGFRVKERLFETQTKYQRLELYETEGFGKLLVLDGTVQLVEMGEESYHEPLVHPVMLAHPN
PRRVLIIGGGDGGTLREVLRHKTVEKAIMVEIDEMVIEVSRIYMNVARGAFEDPRAELIVSDGVEYLKNTDEKFDVIIVD
STDPVGPAKMLFSEGFFRNAYEKLNDNGLYITQAGSVYLFTNELLDAYRDMGKVFDEVHYFSFPVIGYASPWSFLVGVKG
DINFRKVDLQRAKELKLYYYDPERHETLFQMPKYVRELLEKV

Sequences:

>Translated_282_residues
MGFNEQENAFIEWYPRGYGVGFRVKERLFETQTKYQRLELYETEGFGKLLVLDGTVQLVEMGEESYHEPLVHPVMLAHPN
PRRVLIIGGGDGGTLREVLRHKTVEKAIMVEIDEMVIEVSRIYMNVARGAFEDPRAELIVSDGVEYLKNTDEKFDVIIVD
STDPVGPAKMLFSEGFFRNAYEKLNDNGLYITQAGSVYLFTNELLDAYRDMGKVFDEVHYFSFPVIGYASPWSFLVGVKG
DINFRKVDLQRAKELKLYYYDPERHETLFQMPKYVRELLEKV
>Mature_281_residues
GFNEQENAFIEWYPRGYGVGFRVKERLFETQTKYQRLELYETEGFGKLLVLDGTVQLVEMGEESYHEPLVHPVMLAHPNP
RRVLIIGGGDGGTLREVLRHKTVEKAIMVEIDEMVIEVSRIYMNVARGAFEDPRAELIVSDGVEYLKNTDEKFDVIIVDS
TDPVGPAKMLFSEGFFRNAYEKLNDNGLYITQAGSVYLFTNELLDAYRDMGKVFDEVHYFSFPVIGYASPWSFLVGVKGD
INFRKVDLQRAKELKLYYYDPERHETLFQMPKYVRELLEKV

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family

Homologues:

Organism=Homo sapiens, GI63253298, Length=280, Percent_Identity=33.5714285714286, Blast_Score=174, Evalue=1e-43,
Organism=Escherichia coli, GI1786312, Length=269, Percent_Identity=36.4312267657993, Blast_Score=193, Evalue=9e-51,
Organism=Caenorhabditis elegans, GI25154106, Length=270, Percent_Identity=40.7407407407407, Blast_Score=180, Evalue=8e-46,
Organism=Caenorhabditis elegans, GI212645616, Length=322, Percent_Identity=34.7826086956522, Blast_Score=161, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6323175, Length=278, Percent_Identity=37.7697841726619, Blast_Score=180, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6325326, Length=268, Percent_Identity=33.955223880597, Blast_Score=168, Evalue=7e-43,
Organism=Drosophila melanogaster, GI24645443, Length=267, Percent_Identity=34.8314606741573, Blast_Score=176, Evalue=1e-44,
Organism=Drosophila melanogaster, GI21355941, Length=209, Percent_Identity=35.8851674641148, Blast_Score=140, Evalue=8e-34,
Organism=Drosophila melanogaster, GI24663238, Length=188, Percent_Identity=28.7234042553192, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21358269, Length=188, Percent_Identity=28.7234042553192, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SPEE_THEON (B6YXJ9)

Other databases:

- EMBL:   CP000855
- RefSeq:   YP_002307709.1
- ProteinModelPortal:   B6YXJ9
- GeneID:   7018350
- GenomeReviews:   CP000855_GR
- KEGG:   ton:TON_1322
- HOGENOM:   HBG635113
- OMA:   DGHIYNE
- ProtClustDB:   PRK00811
- HAMAP:   MF_00198
- InterPro:   IPR001045
- PANTHER:   PTHR11558
- TIGRFAMs:   TIGR00417

Pfam domain/function: PF01564 Spermine_synth

EC number: =2.5.1.16

Molecular weight: Translated: 32658; Mature: 32527

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: BINDING 36-36 BINDING 91-91 BINDING 111-111 BINDING 160-160 BINDING 163-163

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFNEQENAFIEWYPRGYGVGFRVKERLFETQTKYQRLELYETEGFGKLLVLDGTVQLVE
CCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCEEEEECCCEEEEC
MGEESYHEPLVHPVMLAHPNPRRVLIIGGGDGGTLREVLRHKTVEKAIMVEIDEMVIEVS
CCCHHHCCCCCCCEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RIYMNVARGAFEDPRAELIVSDGVEYLKNTDEKFDVIIVDSTDPVGPAKMLFSEGFFRNA
HHHHHHHHCCCCCCHHHEEHHCCHHHHHCCCCCEEEEEEECCCCCCHHHHHHHCCHHHHH
YEKLNDNGLYITQAGSVYLFTNELLDAYRDMGKVFDEVHYFSFPVIGYASPWSFLVGVKG
HHHHCCCCEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCEEEECCC
DINFRKVDLQRAKELKLYYYDPERHETLFQMPKYVRELLEKV
CCCEEEEEHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
GFNEQENAFIEWYPRGYGVGFRVKERLFETQTKYQRLELYETEGFGKLLVLDGTVQLVE
CCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCEEEEECCCEEEEC
MGEESYHEPLVHPVMLAHPNPRRVLIIGGGDGGTLREVLRHKTVEKAIMVEIDEMVIEVS
CCCHHHCCCCCCCEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RIYMNVARGAFEDPRAELIVSDGVEYLKNTDEKFDVIIVDSTDPVGPAKMLFSEGFFRNA
HHHHHHHHCCCCCCHHHEEHHCCHHHHHCCCCCEEEEEEECCCCCCHHHHHHHCCHHHHH
YEKLNDNGLYITQAGSVYLFTNELLDAYRDMGKVFDEVHYFSFPVIGYASPWSFLVGVKG
HHHHCCCCEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCEEEECCC
DINFRKVDLQRAKELKLYYYDPERHETLFQMPKYVRELLEKV
CCCEEEEEHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA