Click here to switch to the map view.

The map label for this gene is tfb [H]

Identifier: 212224386

GI number: 212224386

Start: 1127891

End: 1128802

Strand: Reverse

Name: tfb [H]

Synonym: TON_1237

Alternate gene names: 212224386

Gene position: 1128802-1127891 (Counterclockwise)

Preceding gene: 212224387

Following gene: 212224385

Centisome position: 61.1

GC content: 56.69

Gene sequence:

>912_bases
TTGGCTGATAAGAACATCTGTCCGGTTTGTGGTTCCGATAAGCTGTTCTACGATCCGCGGAGGGGCGAGATCGTCTGTTC
TGTCTGCGGTTATGTCGTTCAGCAGAATGTTATCGATGAGGGTCCCGAATGGCGCGCCTTTGACCCCGATCAAAGGACAA
AGCGCGCCAGGACCGGCGCGCCCATGACATTGATGATCCACGATAAGGGCCTCTCCACCGATATAGACTGGCGCGATAGG
GACATTCACGGCAACCAGATTACGGGAATGTATCGTACCAAGATGCGCAGGCTCAGGATGTGGCAGCGCAGGATGAGAAT
AAACGACGCCGCCGAGCGTAACTTGGCATTCGCCCTGAGCGAGCTTGACAGGATGGCTGCCCAGATGATGCTGCCGAGGC
GCGTTAAGGAGGTGGCCGCCTCGCTCTACAGGAAAGCCGTCATGAAGAAGCTCATCCGCGGAAGGTCAATCGAGGGAATG
GTCTCGGCTGCCCTCTATGCCGCCTGCAGGATGGAGGGCATTCCAAGGACGCTCGACGAGATTGCTGCAGTTTCGAAGGT
AACCAAGAAGGAGATAGGAAGGAGCTACCGCTTCTTGGCTAGAGGCCTTGGGCTGAACCTTAGGCCGACGAGTCCGATCG
AGTACATTGACCGCTTCGGCGATTCCCTCGACGTGAGTCAGAGAACCAAGAAGCGCGCCAAGGAGATACTTCAGGAGGCC
ATCAAGAGGGGAATAACAAGTGGAAAAGGACCAACTGGTTTAGCTGCCGCGGCTCTGTACGTTGCCTCACTCCTCGAGGG
GGAGAAGAAGACCCAGCGCGAGGTAGCCGAGGTAGCCCACGTGACCGAAGTTACTGTCAGGAACCGCTACAAGGAGCTCG
TCGAGAAGCTCGGCATAAACGTCCCGATCTGA

Upstream 100 bases:

>100_bases
TACCAAGCGCAAAAAGACCAAGCCGAATCACGGGAAGAAGCGAAGAGCTAAGGGCGGAACGGGTAGACGCCCTGCCCCCA
GAAAAAGGGGGTGAGAACTT

Downstream 100 bases:

>100_bases
GGGATGGCCTTGCTGATAGCGGTGATGAGCGACACACACTACGGCGACAAGACAAGAAATTTCCCTTCCCTTCTTTTCCG
AGAGCTTGAGAAGAGAGGGC

Product: transcription initiation factor IIB

Products: NA

Alternate protein names: TFIIB 2 [H]

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MADKNICPVCGSDKLFYDPRRGEIVCSVCGYVVQQNVIDEGPEWRAFDPDQRTKRARTGAPMTLMIHDKGLSTDIDWRDR
DIHGNQITGMYRTKMRRLRMWQRRMRINDAAERNLAFALSELDRMAAQMMLPRRVKEVAASLYRKAVMKKLIRGRSIEGM
VSAALYAACRMEGIPRTLDEIAAVSKVTKKEIGRSYRFLARGLGLNLRPTSPIEYIDRFGDSLDVSQRTKKRAKEILQEA
IKRGITSGKGPTGLAAAALYVASLLEGEKKTQREVAEVAHVTEVTVRNRYKELVEKLGINVPI

Sequences:

>Translated_303_residues
MADKNICPVCGSDKLFYDPRRGEIVCSVCGYVVQQNVIDEGPEWRAFDPDQRTKRARTGAPMTLMIHDKGLSTDIDWRDR
DIHGNQITGMYRTKMRRLRMWQRRMRINDAAERNLAFALSELDRMAAQMMLPRRVKEVAASLYRKAVMKKLIRGRSIEGM
VSAALYAACRMEGIPRTLDEIAAVSKVTKKEIGRSYRFLARGLGLNLRPTSPIEYIDRFGDSLDVSQRTKKRAKEILQEA
IKRGITSGKGPTGLAAAALYVASLLEGEKKTQREVAEVAHVTEVTVRNRYKELVEKLGINVPI
>Mature_302_residues
ADKNICPVCGSDKLFYDPRRGEIVCSVCGYVVQQNVIDEGPEWRAFDPDQRTKRARTGAPMTLMIHDKGLSTDIDWRDRD
IHGNQITGMYRTKMRRLRMWQRRMRINDAAERNLAFALSELDRMAAQMMLPRRVKEVAASLYRKAVMKKLIRGRSIEGMV
SAALYAACRMEGIPRTLDEIAAVSKVTKKEIGRSYRFLARGLGLNLRPTSPIEYIDRFGDSLDVSQRTKKRAKEILQEAI
KRGITSGKGPTGLAAAALYVASLLEGEKKTQREVAEVAHVTEVTVRNRYKELVEKLGINVPI

Specific function: Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre- initiation complex (DNA-TBP-TFIIB) [H]

COG id: COG1405

COG function: function code K; Transcription initiation factor TFIIIB, Brf1 subunit/Transcription initiation factor TFIIB

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TFIIB-type zinc finger [H]

Homologues:

Organism=Homo sapiens, GI4504193, Length=286, Percent_Identity=33.5664335664336, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI22035556, Length=176, Percent_Identity=30.1136363636364, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17564892, Length=295, Percent_Identity=32.8813559322034, Blast_Score=173, Evalue=8e-44,
Organism=Caenorhabditis elegans, GI17531397, Length=290, Percent_Identity=24.8275862068966, Blast_Score=100, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6325343, Length=316, Percent_Identity=26.2658227848101, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6321685, Length=169, Percent_Identity=24.8520710059172, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI17136758, Length=290, Percent_Identity=33.7931034482759, Blast_Score=168, Evalue=5e-42,
Organism=Drosophila melanogaster, GI281361961, Length=162, Percent_Identity=32.0987654320988, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24647658, Length=162, Percent_Identity=32.0987654320988, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006670
- InterPro:   IPR011028
- InterPro:   IPR013763
- InterPro:   IPR000812
- InterPro:   IPR013150
- InterPro:   IPR013137 [H]

Pfam domain/function: PF00382 TFIIB; PF08271 TFIIB_Zn_Ribbon [H]

EC number: NA

Molecular weight: Translated: 34301; Mature: 34170

Theoretical pI: Translated: 10.45; Mature: 10.45

Prosite motif: PS51134 ZF_TFIIB ; PS00782 TFIIB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCC
>Mature Secondary Structure 
ADKNICPVCGSDKLFYDPRRGEIVCSVCGYVVQQNVIDEGPEWRAFDPDQRTKRARTGA
CCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCC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CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11453461 [H]