Click here to switch to the map view.

The map label for this gene is kipI [H]

Identifier: 212224199

GI number: 212224199

Start: 970797

End: 971483

Strand: Reverse

Name: kipI [H]

Synonym: TON_1050

Alternate gene names: 212224199

Gene position: 971483-970797 (Counterclockwise)

Preceding gene: 212224200

Following gene: 212224198

Centisome position: 52.58

GC content: 53.71

Gene sequence:

>687_bases
ATGGTCGAGTTCAAACCAGCCGGCGACTCAGCGCTTGTTATCTCCTTCGGCGAGGTAATAGATGAGAATGTAAACAGAAA
AGTCCACGCCGTGGCGAGAGCCATCGAAGAAGCTAGTCCTCAGTGGCTCATCGAGGTTGTCCCAACTTACTCAACCGTTT
ACGTCTACTACGATCCGTTCATGGTGAGCTATACCGAGGTCGCCGAGGCAGTGAAACATTTCCTCTCCGCTGAGCCTGAT
GAGGAGAAGTCGAGAATAGTCGAGATTCCAACGGTTTACGGCGGTGAATTCGGGCCTGACATCGAGTTCGTCGCTCAATA
CAATGGCCTCAGCGTGGATGACGTCATCGATATCCACTCGAAGCCTTTGTACAGGGTCTACATGCTAGGCTTCACCCCAG
GCTTTGCCTATCTGGGTGGAATGGACGAGAAGATAGCCACCCCCCGCTTGGAAAAACCGAGGCTGAAAGTTCCCGCCGGA
AGCGTGGGCATAGCTGGAAAGCAGACGGGCATATATCCCCTCGAAAGTCCCGGTGGCTGGCGGCTTATCGGCAGAACTCC
TCTGAAGCTTTTTGATCCAAGGAAAGACCCACCGACGCTCCTCCAGCCTGGCGACTACGTGAAGTTTGTGCCGATAAGTG
AGGAGGAGTTCTGGGAGCTTTACAGAGGGGGTCAGGACAATGATTGA

Upstream 100 bases:

>100_bases
GACAACCCGAAGGCAGTGGAGATAGCAGCTTATATCAGGCGCGTCCTCGAAGATGAGGGCATCAATGTAGTGCCTATGGG
TGAGTTCATAAGGTGAGAGC

Downstream 100 bases:

>100_bases
GCTTCTCCAAGTCCCCTCGCTCCTAACCGTCCAAGATCTTGGCAGAACCGGCTACAGAAAGCTTGGCGTCCCAGTAAGCG
GCGTCATGGACGAAGTGAGC

Product: protein

Products: NA

Alternate protein names: Sporulation inhibitor kipI [H]

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MVEFKPAGDSALVISFGEVIDENVNRKVHAVARAIEEASPQWLIEVVPTYSTVYVYYDPFMVSYTEVAEAVKHFLSAEPD
EEKSRIVEIPTVYGGEFGPDIEFVAQYNGLSVDDVIDIHSKPLYRVYMLGFTPGFAYLGGMDEKIATPRLEKPRLKVPAG
SVGIAGKQTGIYPLESPGGWRLIGRTPLKLFDPRKDPPTLLQPGDYVKFVPISEEEFWELYRGGQDND

Sequences:

>Translated_228_residues
MVEFKPAGDSALVISFGEVIDENVNRKVHAVARAIEEASPQWLIEVVPTYSTVYVYYDPFMVSYTEVAEAVKHFLSAEPD
EEKSRIVEIPTVYGGEFGPDIEFVAQYNGLSVDDVIDIHSKPLYRVYMLGFTPGFAYLGGMDEKIATPRLEKPRLKVPAG
SVGIAGKQTGIYPLESPGGWRLIGRTPLKLFDPRKDPPTLLQPGDYVKFVPISEEEFWELYRGGQDND
>Mature_228_residues
MVEFKPAGDSALVISFGEVIDENVNRKVHAVARAIEEASPQWLIEVVPTYSTVYVYYDPFMVSYTEVAEAVKHFLSAEPD
EEKSRIVEIPTVYGGEFGPDIEFVAQYNGLSVDDVIDIHSKPLYRVYMLGFTPGFAYLGGMDEKIATPRLEKPRLKVPAG
SVGIAGKQTGIYPLESPGGWRLIGRTPLKLFDPRKDPPTLLQPGDYVKFVPISEEEFWELYRGGQDND

Specific function: Is a potent inhibitor of the autophosphorylation reaction of kinase A and its reverse reaction, but does not inhibit phosphate transfer to the Spo0F response regulator once kinase A is phosphorylated. KipI is an inhibitor of the catalytic domain of kinase

COG id: COG2049

COG function: function code E; Allophanate hydrolase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786929, Length=208, Percent_Identity=42.3076923076923, Blast_Score=169, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6319685, Length=252, Percent_Identity=22.6190476190476, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003833
- InterPro:   IPR020899
- InterPro:   IPR010016
- InterPro:   IPR002130 [H]

Pfam domain/function: PF02682 AHS1 [H]

EC number: NA

Molecular weight: Translated: 25481; Mature: 25481

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEFKPAGDSALVISFGEVIDENVNRKVHAVARAIEEASPQWLIEVVPTYSTVYVYYDPF
CCEECCCCCCEEEEEHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEECCCEEEEEEECCC
MVSYTEVAEAVKHFLSAEPDEEKSRIVEIPTVYGGEFGPDIEFVAQYNGLSVDDVIDIHS
EEEHHHHHHHHHHHHCCCCCCCCCEEEECCEEECCCCCCCEEEEEECCCCCHHHHHHCCC
KPLYRVYMLGFTPGFAYLGGMDEKIATPRLEKPRLKVPAGSVGIAGKQTGIYPLESPGGW
CCEEEEEEEECCCCHHHHCCCCHHHCCCCCCCCEEECCCCCCCCCCCCCCCEECCCCCCE
RLIGRTPLKLFDPRKDPPTLLQPGDYVKFVPISEEEFWELYRGGQDND
EEECCCCHHEECCCCCCCCCCCCCCEEEEEECCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MVEFKPAGDSALVISFGEVIDENVNRKVHAVARAIEEASPQWLIEVVPTYSTVYVYYDPF
CCEECCCCCCEEEEEHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEECCCEEEEEEECCC
MVSYTEVAEAVKHFLSAEPDEEKSRIVEIPTVYGGEFGPDIEFVAQYNGLSVDDVIDIHS
EEEHHHHHHHHHHHHCCCCCCCCCEEEECCEEECCCCCCCEEEEEECCCCCHHHHHHCCC
KPLYRVYMLGFTPGFAYLGGMDEKIATPRLEKPRLKVPAGSVGIAGKQTGIYPLESPGGW
CCEEEEEEEECCCCHHHHCCCCHHHCCCCCCCCEEECCCCCCCCCCCCCCCEECCCCCCE
RLIGRTPLKLFDPRKDPPTLLQPGDYVKFVPISEEEFWELYRGGQDND
EEECCCCHHEECCCCCCCCCCCCCCEEEEEECCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377; 10568751 [H]