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The map label for this gene is thiB [H]

Identifier: 212224112

GI number: 212224112

Start: 886790

End: 887932

Strand: Reverse

Name: thiB [H]

Synonym: TON_0963

Alternate gene names: 212224112

Gene position: 887932-886790 (Counterclockwise)

Preceding gene: 212224113

Following gene: 212224111

Centisome position: 48.06

GC content: 56.43

Gene sequence:

>1143_bases
ATGAGGAAACTTGGCATCGCACTCCTAGTAGTGCTCCTTGTGGGGCTTTTCGCGGTTCCAAAGCCCGCTGCTGCCCAGGA
AGAGCTGACTGTTTACTCCTACGACAGCATAGAGTGGTGGATGAAGGAGATAGTTCCGATATTCGAGGAGAAGTACGGTG
TTAAGGTCAACCTCGTCCTCATAGGTGACGCTGGAGAGGTCCTCAACAGGCTCATCCTCGAGAAGGACAGCCCGCAGGCT
GATGTCGTCGTCGGCATAGACAACAGCTACCTCGCCAAGGCTATAGATGCAGGCGTTCTCGAGCCATATAAGCCAGAGAA
CGCCGACGTTATTCCTGACTGGATAATCGAGAAGTTTGACCCAACCTTCCACCTCACACCCTACGACTACGGATACATAG
CTATCAACTACCGCAAGGACATGGTTCAGAACCCGCCGACCAGCCTCGAAGACCTCATCAAGCCCGAGTGGAAGGGCAAG
CTCATCATCGAGGATCCGAGGACCAGCTCGCCGGGAATGGCATTCCTCCTGTGGACGATAGCCGTTTATGGCGACGACTG
GCTCTACTACTGGGAGAAGCTCAAGGAGAACGATGTCCAGATTGTCGAGGGCTGGAGCGCGGCCTGGGGAGCTTTCACCA
AGGGCGAGTATCCGCTCGTGCTCAGCTACGCAACTTCCCCTGCTGCAACCGTTTACTATGAGAACAACACCAACATCGGC
GCGGTCGCCTTTAAGGAGGGTAACTACCTCCAGATCGAGGGTGCTGGAATAGTCAAGGGCGCCAAGCACCCAGAGCTCGC
CAAGAAGTTCATAGAGTTCCTCATCAGCGAGGAGGCCCAGGAGAAGCTTCCGGTCAACCAGTGGATGTACCCTGTCAACA
AGAACGTCCAGCTTCCTGAGGTCTTCCAGTACGCGGTTGAGGTCGACAAGCCCGTCACCGTTGACCCGGCCGAGATAGAG
AAGAACTACGAGGCCTGGCTCAAGCAGTGGACCGAACTCATGGTCGAGGGCAAGAGCGCTGACGAGATACTCGGCAAGAC
GACCACCGAAACCAGCGGGGAAACTGATAACGCCGGCATCTGCGGACCGGCCCTGATAGTCGGCCTTGCCATAGTGCCGC
TCCTCCTTAGGAGGAGGCGCTGA

Upstream 100 bases:

>100_bases
TCTGACTTTTTCAAGGTCACAATTCTGGGAGGCAAAGCTTTATTAGGCATCGTATTACCACATTATTGAAAATCACAACC
GAGTATTTGGAGGTGATTCG

Downstream 100 bases:

>100_bases
ATTCTTCTTCCCTTTTCTTCCCACCGTAACTTTTATAAGAGCGCCCTTTAACCACTAACCCGAGGGCCCGTAGCCTAGCA
GGATAGGGCGCCGGCCTTCT

Product: thiamin-binding periplasmic protein precursor

Products: ADP; phosphate; thiamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MRKLGIALLVVLLVGLFAVPKPAAAQEELTVYSYDSIEWWMKEIVPIFEEKYGVKVNLVLIGDAGEVLNRLILEKDSPQA
DVVVGIDNSYLAKAIDAGVLEPYKPENADVIPDWIIEKFDPTFHLTPYDYGYIAINYRKDMVQNPPTSLEDLIKPEWKGK
LIIEDPRTSSPGMAFLLWTIAVYGDDWLYYWEKLKENDVQIVEGWSAAWGAFTKGEYPLVLSYATSPAATVYYENNTNIG
AVAFKEGNYLQIEGAGIVKGAKHPELAKKFIEFLISEEAQEKLPVNQWMYPVNKNVQLPEVFQYAVEVDKPVTVDPAEIE
KNYEAWLKQWTELMVEGKSADEILGKTTTETSGETDNAGICGPALIVGLAIVPLLLRRRR

Sequences:

>Translated_380_residues
MRKLGIALLVVLLVGLFAVPKPAAAQEELTVYSYDSIEWWMKEIVPIFEEKYGVKVNLVLIGDAGEVLNRLILEKDSPQA
DVVVGIDNSYLAKAIDAGVLEPYKPENADVIPDWIIEKFDPTFHLTPYDYGYIAINYRKDMVQNPPTSLEDLIKPEWKGK
LIIEDPRTSSPGMAFLLWTIAVYGDDWLYYWEKLKENDVQIVEGWSAAWGAFTKGEYPLVLSYATSPAATVYYENNTNIG
AVAFKEGNYLQIEGAGIVKGAKHPELAKKFIEFLISEEAQEKLPVNQWMYPVNKNVQLPEVFQYAVEVDKPVTVDPAEIE
KNYEAWLKQWTELMVEGKSADEILGKTTTETSGETDNAGICGPALIVGLAIVPLLLRRRR
>Mature_380_residues
MRKLGIALLVVLLVGLFAVPKPAAAQEELTVYSYDSIEWWMKEIVPIFEEKYGVKVNLVLIGDAGEVLNRLILEKDSPQA
DVVVGIDNSYLAKAIDAGVLEPYKPENADVIPDWIIEKFDPTFHLTPYDYGYIAINYRKDMVQNPPTSLEDLIKPEWKGK
LIIEDPRTSSPGMAFLLWTIAVYGDDWLYYWEKLKENDVQIVEGWSAAWGAFTKGEYPLVLSYATSPAATVYYENNTNIG
AVAFKEGNYLQIEGAGIVKGAKHPELAKKFIEFLISEEAQEKLPVNQWMYPVNKNVQLPEVFQYAVEVDKPVTVDPAEIE
KNYEAWLKQWTELMVEGKSADEILGKTTTETSGETDNAGICGPALIVGLAIVPLLLRRRR

Specific function: Part of the ABC transporter complex thiBPQ involved in thiamine import [H]

COG id: COG4143

COG function: function code H; ABC-type thiamine transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1786255, Length=314, Percent_Identity=39.4904458598726, Blast_Score=223, Evalue=2e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR006061
- InterPro:   IPR005948
- InterPro:   IPR005967 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 42727; Mature: 42727

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLGIALLVVLLVGLFAVPKPAAAQEELTVYSYDSIEWWMKEIVPIFEEKYGVKVNLVL
CCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCEEEEEE
IGDAGEVLNRLILEKDSPQADVVVGIDNSYLAKAIDAGVLEPYKPENADVIPDWIIEKFD
ECCHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCC
PTFHLTPYDYGYIAINYRKDMVQNPPTSLEDLIKPEWKGKLIIEDPRTSSPGMAFLLWTI
CCEEECCCCCEEEEEEEEHHHHCCCCCCHHHHCCCCCCCEEEEECCCCCCCCHHHEEEEH
AVYGDDWLYYWEKLKENDVQIVEGWSAAWGAFTKGEYPLVLSYATSPAATVYYENNTNIG
HHHCCCHHHHHHHHCCCCEEEEECCCHHHCCCCCCCCCEEEEECCCCEEEEEEECCCCEE
AVAFKEGNYLQIEGAGIVKGAKHPELAKKFIEFLISEEAQEKLPVNQWMYPVNKNVQLPE
EEEEECCCEEEEECCCEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHEECCCCCCCCHHH
VFQYAVEVDKPVTVDPAEIEKNYEAWLKQWTELMVEGKSADEILGKTTTETSGETDNAGI
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCC
CGPALIVGLAIVPLLLRRRR
CHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRKLGIALLVVLLVGLFAVPKPAAAQEELTVYSYDSIEWWMKEIVPIFEEKYGVKVNLVL
CCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCEEEEEE
IGDAGEVLNRLILEKDSPQADVVVGIDNSYLAKAIDAGVLEPYKPENADVIPDWIIEKFD
ECCHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCC
PTFHLTPYDYGYIAINYRKDMVQNPPTSLEDLIKPEWKGKLIIEDPRTSSPGMAFLLWTI
CCEEECCCCCEEEEEEEEHHHHCCCCCCHHHHCCCCCCCEEEEECCCCCCCCHHHEEEEH
AVYGDDWLYYWEKLKENDVQIVEGWSAAWGAFTKGEYPLVLSYATSPAATVYYENNTNIG
HHHCCCHHHHHHHHCCCCEEEEECCCHHHCCCCCCCCCEEEEECCCCEEEEEEECCCCEE
AVAFKEGNYLQIEGAGIVKGAKHPELAKKFIEFLISEEAQEKLPVNQWMYPVNKNVQLPE
EEEEECCCEEEEECCCEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHEECCCCCCCCHHH
VFQYAVEVDKPVTVDPAEIEKNYEAWLKQWTELMVEGKSADEILGKTTTETSGETDNAGI
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCC
CGPALIVGLAIVPLLLRRRR
CHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; thiamine [Periplasm]; H2O [C]

Specific reaction: ATP + thiamine [Periplasm] + H2O = ADP + phosphate + thiamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1630901; 9278503; 9535878 [H]