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The map label for this gene is infB [H]

Identifier: 212224097

GI number: 212224097

Start: 874248

End: 876041

Strand: Reverse

Name: infB [H]

Synonym: TON_0948

Alternate gene names: 212224097

Gene position: 876041-874248 (Counterclockwise)

Preceding gene: 212224100

Following gene: 212224094

Centisome position: 47.41

GC content: 56.41

Gene sequence:

>1794_bases
ATGAAGAGGATTAGACAGCCGATCATAGCGGTTCTCGGTCACGTTGACCATGGAAAAACCACTCTCCTTGACAGGATCAG
GCGCACAAACGTCGCCGGCAAAGAGGCCGGCGGAATAACCCAGCACATTGGTGCAACTGAGGTTCCGATTGAGACCGTAA
AGAACCTCGCCGGTCCGCTCATCAAACTCTGGAAGGGCGAGATAAAGCTCCCTGGCCTGCTTTTCATCGACACGCCCGGT
CACGAGGCCTTCACGAGCCTGCGCGCGAGGGGTGGTAGCCTCGCGGATCTTGCCGTCCTCGTTGTTGATATAAACGAGGG
CTTCCAGCCCCAGACTATAGAGAGCATCGAGATCCTCCGGAAGAACAGAACGCCATTCATCGTTGCAGCCAACAAGATAG
ACAGGATTAAAGGATGGAAGATTGAGAAGGACGAGCCTTTCCTCGTGAACATCAAGAAGCAGGATCAGAGGGCCCAGCAG
GAGCTTGAGACCAAGCTCTGGGAGCTGATAGGCAAGTTCTACGAGATGGGCTTCCAGGCGAACCGCTTTGATCGCGTCCA
GAACTTCACCCGCGAGCTGGCGATAGTTCCGATTTCGGCCAAGTACGGCATAGGCGTCCCCGAGCTGCTCGTCCTTATCG
CGGGTCTCAGCCAGAAGTACTTAGAGGAGAAGCTCAAGATTGAAGTTGAAGGCCCGGCACGCGGCACAATCCTCGAAGTC
AGGGAAGAGATCGGTCTCGGAACTACCATAGACGTCATAATCTATGACGGAACGCTTCACAAAGATGATACAATCGTCGT
GGGTGGCAAGGACAAGGCGATAGTGACGAAAATCCGCGCTCTGCTCAAGCCTAAGCCCCTCGACGAGATACGTGACCCGC
GCTTCCGCTTCGACCAGGTTGACGAGGTTACAGCCGCTGCGGGTGTCAAGATAGCTGCTCCTGGCCTTGAGGAGGCCCTT
GCAGGATCGCCGGTCATCGCCGCTCGCTCCGAGGAGGAGGTCGAGAAGGCCAAACAGGAAATACTCAGCCAGATACAGAG
CGTTGTCATAAGCACCGGCAAGGTCGGTGTGATAGTCAAGGCCGATACCCTCGGCTCTCTGGAGGCCCTTAGCAAGGAGC
TCCAGGAGAAGAACATCCCAATAAGGAAGGCCGACGTCGGGAACATCAGCAAGACCGACGTCATGGAGGCCCTGAGCGTC
AAGGACGAGGATCCAAAGTACGGCGTCGTCCTCGGCTTCAACGTCAAGGTAAACGAGGACGCTGAAGAGGTTGCTAAGGC
CAGGGGCGTGCCCATATTCACAGGCAACATCATCTACAAGCTCATTGAGGACTACGAGGCCTGGGTCAAGGGCGAGGAGG
AGAAGAAGAAGCGCGAGCTACTCAGCAAGGTCACCTTCCCGGGCGTCATCAGGCTCTATCCTGACGAGCGCTATGTCTTC
AGGAGGAGCCATCCTGCGATAGTCGGTATCGAGGTCGTTGAGGGCAGGATAAGGCCCGGCGTCACGCTCATCAAGCAGAA
CGGCCAGAAGGTCGGCGTTATTAAGTCCATCAAGAACCGCAACGACTTCGTGCAGGAGGCCAAGAAGGGCGAGGCGGTGG
CAATAGCCATCGAGGGCGCGATAGTCGGCAGGCACATCCACCCAGGTGAGACGCTCTACGTTGACCTGAGCAAGAACGAC
GTCATAATCCTGGCGAAGCAGCTCAAGAACGAGCTCGACGAGACTGACATAAAAGCCCTGAAGATGACGGCGAAGGTGAA
GGCCCAACAGGATCCGTTCTGGAAGGCGGTTTGA

Upstream 100 bases:

>100_bases
TTTTGCCGCTTCTCTGGAATTACCACCCGGACTTTTAATCCTTCCGCCCGAACGAACCCTTTAAAAACTCACGCCGGAGT
ATCTTCCAGAGGTGAGGGAA

Downstream 100 bases:

>100_bases
CCTCTACTTCTTTTCTAACATTATTACCGCCTCAACATGCGGCGTATGCGGGAACATATCGGCCAGAACGGCCTCCTCGA
CTTGGTAGGCCTTCTTTAAG

Product: translation initiation factor IF-2

Products: NA

Alternate protein names: Pko IF2 intein [H]

Number of amino acids: Translated: 597; Mature: 597

Protein sequence:

>597_residues
MKRIRQPIIAVLGHVDHGKTTLLDRIRRTNVAGKEAGGITQHIGATEVPIETVKNLAGPLIKLWKGEIKLPGLLFIDTPG
HEAFTSLRARGGSLADLAVLVVDINEGFQPQTIESIEILRKNRTPFIVAANKIDRIKGWKIEKDEPFLVNIKKQDQRAQQ
ELETKLWELIGKFYEMGFQANRFDRVQNFTRELAIVPISAKYGIGVPELLVLIAGLSQKYLEEKLKIEVEGPARGTILEV
REEIGLGTTIDVIIYDGTLHKDDTIVVGGKDKAIVTKIRALLKPKPLDEIRDPRFRFDQVDEVTAAAGVKIAAPGLEEAL
AGSPVIAARSEEEVEKAKQEILSQIQSVVISTGKVGVIVKADTLGSLEALSKELQEKNIPIRKADVGNISKTDVMEALSV
KDEDPKYGVVLGFNVKVNEDAEEVAKARGVPIFTGNIIYKLIEDYEAWVKGEEEKKKRELLSKVTFPGVIRLYPDERYVF
RRSHPAIVGIEVVEGRIRPGVTLIKQNGQKVGVIKSIKNRNDFVQEAKKGEAVAIAIEGAIVGRHIHPGETLYVDLSKND
VIILAKQLKNELDETDIKALKMTAKVKAQQDPFWKAV

Sequences:

>Translated_597_residues
MKRIRQPIIAVLGHVDHGKTTLLDRIRRTNVAGKEAGGITQHIGATEVPIETVKNLAGPLIKLWKGEIKLPGLLFIDTPG
HEAFTSLRARGGSLADLAVLVVDINEGFQPQTIESIEILRKNRTPFIVAANKIDRIKGWKIEKDEPFLVNIKKQDQRAQQ
ELETKLWELIGKFYEMGFQANRFDRVQNFTRELAIVPISAKYGIGVPELLVLIAGLSQKYLEEKLKIEVEGPARGTILEV
REEIGLGTTIDVIIYDGTLHKDDTIVVGGKDKAIVTKIRALLKPKPLDEIRDPRFRFDQVDEVTAAAGVKIAAPGLEEAL
AGSPVIAARSEEEVEKAKQEILSQIQSVVISTGKVGVIVKADTLGSLEALSKELQEKNIPIRKADVGNISKTDVMEALSV
KDEDPKYGVVLGFNVKVNEDAEEVAKARGVPIFTGNIIYKLIEDYEAWVKGEEEKKKRELLSKVTFPGVIRLYPDERYVF
RRSHPAIVGIEVVEGRIRPGVTLIKQNGQKVGVIKSIKNRNDFVQEAKKGEAVAIAIEGAIVGRHIHPGETLYVDLSKND
VIILAKQLKNELDETDIKALKMTAKVKAQQDPFWKAV
>Mature_597_residues
MKRIRQPIIAVLGHVDHGKTTLLDRIRRTNVAGKEAGGITQHIGATEVPIETVKNLAGPLIKLWKGEIKLPGLLFIDTPG
HEAFTSLRARGGSLADLAVLVVDINEGFQPQTIESIEILRKNRTPFIVAANKIDRIKGWKIEKDEPFLVNIKKQDQRAQQ
ELETKLWELIGKFYEMGFQANRFDRVQNFTRELAIVPISAKYGIGVPELLVLIAGLSQKYLEEKLKIEVEGPARGTILEV
REEIGLGTTIDVIIYDGTLHKDDTIVVGGKDKAIVTKIRALLKPKPLDEIRDPRFRFDQVDEVTAAAGVKIAAPGLEEAL
AGSPVIAARSEEEVEKAKQEILSQIQSVVISTGKVGVIVKADTLGSLEALSKELQEKNIPIRKADVGNISKTDVMEALSV
KDEDPKYGVVLGFNVKVNEDAEEVAKARGVPIFTGNIIYKLIEDYEAWVKGEEEKKKRELLSKVTFPGVIRLYPDERYVF
RRSHPAIVGIEVVEGRIRPGVTLIKQNGQKVGVIKSIKNRNDFVQEAKKGEAVAIAIEGAIVGRHIHPGETLYVDLSKND
VIILAKQLKNELDETDIKALKMTAKVKAQQDPFWKAV

Specific function: Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2 [H]

COG id: COG0532

COG function: function code J; Translation initiation factor 2 (IF-2; GTPase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DOD-type homing endonuclease domain [H]

Homologues:

Organism=Homo sapiens, GI84043963, Length=585, Percent_Identity=38.6324786324786, Blast_Score=395, Evalue=1e-110,
Organism=Homo sapiens, GI53729339, Length=264, Percent_Identity=35.2272727272727, Blast_Score=136, Evalue=6e-32,
Organism=Homo sapiens, GI53729337, Length=264, Percent_Identity=35.2272727272727, Blast_Score=136, Evalue=6e-32,
Organism=Escherichia coli, GI1789559, Length=603, Percent_Identity=29.6849087893864, Blast_Score=161, Evalue=1e-40,
Organism=Escherichia coli, GI2367247, Length=142, Percent_Identity=35.2112676056338, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI212656558, Length=584, Percent_Identity=38.013698630137, Blast_Score=378, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI71994658, Length=497, Percent_Identity=26.7605633802817, Blast_Score=118, Evalue=8e-27,
Organism=Saccharomyces cerevisiae, GI6319282, Length=601, Percent_Identity=38.7687188019967, Blast_Score=375, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6324550, Length=589, Percent_Identity=26.9949066213922, Blast_Score=139, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24656849, Length=583, Percent_Identity=38.4219554030875, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI28572034, Length=480, Percent_Identity=28.5416666666667, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI160714833, Length=87, Percent_Identity=49.4252873563218, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI78706572, Length=137, Percent_Identity=34.3065693430657, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: 1150 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003586
- InterPro:   IPR003587
- InterPro:   IPR006142
- InterPro:   IPR004042
- InterPro:   IPR006141
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004544
- InterPro:   IPR015760
- InterPro:   IPR023115
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF11987 IF-2 [H]

EC number: NA

Molecular weight: Translated: 66125; Mature: 66125

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRIRQPIIAVLGHVDHGKTTLLDRIRRTNVAGKEAGGITQHIGATEVPIETVKNLAGPL
CCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHH
IKLWKGEIKLPGLLFIDTPGHEAFTSLRARGGSLADLAVLVVDINEGFQPQTIESIEILR
HHHHCCCCCCCEEEEEECCCHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCHHHHHHHHH
KNRTPFIVAANKIDRIKGWKIEKDEPFLVNIKKQDQRAQQELETKLWELIGKFYEMGFQA
CCCCCEEEECCCCCCCCCCEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCC
NRFDRVQNFTRELAIVPISAKYGIGVPELLVLIAGLSQKYLEEKLKIEVEGPARGTILEV
HHHHHHHHHHHCEEEEEECCCCCCCHHHHHHHHHCCHHHHHHHHEEEEECCCCCCHHHHH
REEIGLGTTIDVIIYDGTLHKDDTIVVGGKDKAIVTKIRALLKPKPLDEIRDPRFRFDQV
HHHCCCCCEEEEEEECCEECCCCEEEECCCCHHHHHHHHHHHCCCCHHHHCCCCCCCCHH
DEVTAAAGVKIAAPGLEEALAGSPVIAARSEEEVEKAKQEILSQIQSVVISTGKVGVIVK
HHHHHHCCCEEECCCHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
ADTLGSLEALSKELQEKNIPIRKADVGNISKTDVMEALSVKDEDPKYGVVLGFNVKVNED
ECCCCHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHCCCCCCCEEEEEEEEEEECCC
AEEVAKARGVPIFTGNIIYKLIEDYEAWVKGEEEKKKRELLSKVTFPGVIRLYPDERYVF
HHHHHHHCCCEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEECCCCCEEE
RRSHPAIVGIEVVEGRIRPGVTLIKQNGQKVGVIKSIKNRNDFVQEAKKGEAVAIAIEGA
ECCCCCEEEEEEECCCCCCCEEEEECCCCEEEHHHHHHCHHHHHHHHCCCCEEEEEEECE
IVGRHIHPGETLYVDLSKNDVIILAKQLKNELDETDIKALKMTAKVKAQQDPFWKAV
EEECEECCCCEEEEEECCCCEEEEEHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKRIRQPIIAVLGHVDHGKTTLLDRIRRTNVAGKEAGGITQHIGATEVPIETVKNLAGPL
CCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHH
IKLWKGEIKLPGLLFIDTPGHEAFTSLRARGGSLADLAVLVVDINEGFQPQTIESIEILR
HHHHCCCCCCCEEEEEECCCHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCHHHHHHHHH
KNRTPFIVAANKIDRIKGWKIEKDEPFLVNIKKQDQRAQQELETKLWELIGKFYEMGFQA
CCCCCEEEECCCCCCCCCCEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCC
NRFDRVQNFTRELAIVPISAKYGIGVPELLVLIAGLSQKYLEEKLKIEVEGPARGTILEV
HHHHHHHHHHHCEEEEEECCCCCCCHHHHHHHHHCCHHHHHHHHEEEEECCCCCCHHHHH
REEIGLGTTIDVIIYDGTLHKDDTIVVGGKDKAIVTKIRALLKPKPLDEIRDPRFRFDQV
HHHCCCCCEEEEEEECCEECCCCEEEECCCCHHHHHHHHHHHCCCCHHHHCCCCCCCCHH
DEVTAAAGVKIAAPGLEEALAGSPVIAARSEEEVEKAKQEILSQIQSVVISTGKVGVIVK
HHHHHHCCCEEECCCHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
ADTLGSLEALSKELQEKNIPIRKADVGNISKTDVMEALSVKDEDPKYGVVLGFNVKVNED
ECCCCHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHCCCCCCCEEEEEEEEEEECCC
AEEVAKARGVPIFTGNIIYKLIEDYEAWVKGEEEKKKRELLSKVTFPGVIRLYPDERYVF
HHHHHHHCCCEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEECCCCCEEE
RRSHPAIVGIEVVEGRIRPGVTLIKQNGQKVGVIKSIKNRNDFVQEAKKGEAVAIAIEGA
ECCCCCEEEEEEECCCCCCCEEEEECCCCEEEHHHHHHCHHHHHHHHCCCCEEEEEEECE
IVGRHIHPGETLYVDLSKNDVIILAKQLKNELDETDIKALKMTAKVKAQQDPFWKAV
EEECEECCCCEEEEEECCCCEEEEEHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; In phosphorus-containing anhydrides [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA