Click here to switch to the map view.

The map label for this gene is trm1

Identifier: 212223796

GI number: 212223796

Start: 604606

End: 605739

Strand: Reverse

Name: trm1

Synonym: TON_0648

Alternate gene names: 212223796

Gene position: 605739-604606 (Counterclockwise)

Preceding gene: 212223797

Following gene: 212223795

Centisome position: 32.79

GC content: 53.79

Gene sequence:

>1134_bases
ATGGAGCTAAAGGAAGTGAGGGAAGGTCTCGCGAGGATTTTGGTTCCAAAGGCCGAGAGGATTTACGATGCGCCAGTCTT
CTACAATCCCCTTATGGCTCTGAACAGAGACATAAGCGTTCTTGCTCTGAAGGCTCTCGGCTCCAGGCGCGTTCTTGATG
CGCTCTCTGCGACAGGGATAAGAGGCATCCGCTACGCCCTCGAAACCCCTGCTGGGGAGATATGGCTCAACGACATAAAT
CCCAATGCGTTCAGGCTTATAGTCGATAACGTGAAACTGAACTTCTCCGGAGAGCTCGAAATCGAAGGGAAGATGGCCGT
TCTCGAGGGAGAAAAAACGTTGGTTGTAACCAACGCCGACGCTAACAGGCTGATGAACGAGCAGTTTAGATACTTCGACT
TCGTTGACTTGGACCCATTCGGCTCGCCGATGGAGTTCCTTGATTCGGCTCTTAGAGCCGTAAAAAGGCGCGGCGTTTTG
GCTATCACTGCGACCGACACTGGAGTCCTCTGCGGAGCCTACAGGAACGCTTGCCTGCGCAAATACCTTGCAGAGCCCAT
AAGGGGCGAGCTCTGCCATGAGGCAGGCCTTAGGATCCTCATTGGGACCCTCGTCAGGTACGCTGCCAAATACGACCTCG
GCGTTGAGGTTCTTCTGGCCTACTACCGTGACCACTACTTCAGGGCCTTCCTCCAGCTGAGGAACGGAGCAAAGACGGCA
GATAAAAGCATGAAGCAGCTTGGCTACCTTTACCAAGACGAGAGCGGAAAGTTCGAGTTTGAAAACACCTTCCTCCCATC
GAAAGCCACCGCCCATGGGCCGCTGTGGCTTGGCCCGCTGAAGAACCAGGAGTTCGTCGAGGAGCTTCATAGGCTGAGTG
CTGAACATCCACTCGGAAGTAAAAAAACACAGAAATTCTTGGGACTCTTAAAAGACGAGCTTGAAGTCCCATTTTTCTAC
GACACCCACGCGGTAGCGAGGAGGCACGGGCTTGAGGTCAGGAAGCTCGCTGGAGTCATCGAGATACTCTACGAGAAGGG
CTATAAGGCAACGAGGACGCACTTCTCGCCGACGGCCCTTAAAACGGACGCACCATTTGAAGAGGTGCTTGAGGCGCTGA
GGGAACTTCAGTGA

Upstream 100 bases:

>100_bases
AGGTCAAGGCCTACTTCAAGCCAGGCCTTCCCGGACAGGCCCTCGGCGACTACGTCGAAATCCTTTAAACCCCCCAACCT
TTTCTTTCTCTGGTGAGATG

Downstream 100 bases:

>100_bases
TTTCATATTGAAGAACGATCGGAATTCCTCAACCTTCCGATAGGCATTTTAACTTTTCTTCGGATTTCTTACGGAGGTGG
GCAAATGGACGAGATGCTTA

Product: N(2),N(2)-dimethylguanosine tRNA methyltransferase

Products: NA

Alternate protein names: tRNA 2,2-dimethylguanosine-26 methyltransferase; tRNA(guanine-26,N(2)-N(2)) methyltransferase; tRNA(m(2,2)G26)dimethyltransferase

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MELKEVREGLARILVPKAERIYDAPVFYNPLMALNRDISVLALKALGSRRVLDALSATGIRGIRYALETPAGEIWLNDIN
PNAFRLIVDNVKLNFSGELEIEGKMAVLEGEKTLVVTNADANRLMNEQFRYFDFVDLDPFGSPMEFLDSALRAVKRRGVL
AITATDTGVLCGAYRNACLRKYLAEPIRGELCHEAGLRILIGTLVRYAAKYDLGVEVLLAYYRDHYFRAFLQLRNGAKTA
DKSMKQLGYLYQDESGKFEFENTFLPSKATAHGPLWLGPLKNQEFVEELHRLSAEHPLGSKKTQKFLGLLKDELEVPFFY
DTHAVARRHGLEVRKLAGVIEILYEKGYKATRTHFSPTALKTDAPFEEVLEALRELQ

Sequences:

>Translated_377_residues
MELKEVREGLARILVPKAERIYDAPVFYNPLMALNRDISVLALKALGSRRVLDALSATGIRGIRYALETPAGEIWLNDIN
PNAFRLIVDNVKLNFSGELEIEGKMAVLEGEKTLVVTNADANRLMNEQFRYFDFVDLDPFGSPMEFLDSALRAVKRRGVL
AITATDTGVLCGAYRNACLRKYLAEPIRGELCHEAGLRILIGTLVRYAAKYDLGVEVLLAYYRDHYFRAFLQLRNGAKTA
DKSMKQLGYLYQDESGKFEFENTFLPSKATAHGPLWLGPLKNQEFVEELHRLSAEHPLGSKKTQKFLGLLKDELEVPFFY
DTHAVARRHGLEVRKLAGVIEILYEKGYKATRTHFSPTALKTDAPFEEVLEALRELQ
>Mature_377_residues
MELKEVREGLARILVPKAERIYDAPVFYNPLMALNRDISVLALKALGSRRVLDALSATGIRGIRYALETPAGEIWLNDIN
PNAFRLIVDNVKLNFSGELEIEGKMAVLEGEKTLVVTNADANRLMNEQFRYFDFVDLDPFGSPMEFLDSALRAVKRRGVL
AITATDTGVLCGAYRNACLRKYLAEPIRGELCHEAGLRILIGTLVRYAAKYDLGVEVLLAYYRDHYFRAFLQLRNGAKTA
DKSMKQLGYLYQDESGKFEFENTFLPSKATAHGPLWLGPLKNQEFVEELHRLSAEHPLGSKKTQKFLGLLKDELEVPFFY
DTHAVARRHGLEVRKLAGVIEILYEKGYKATRTHFSPTALKTDAPFEEVLEALRELQ

Specific function: Dimethylates a single guanine residue at position 26 of a number of tRNAs using S-adenosyl-L-methionine as donor of the methyl groups

COG id: COG1867

COG function: function code J; N2,N2-dimethylguanosine tRNA methyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TRM1 family

Homologues:

Organism=Homo sapiens, GI8923219, Length=359, Percent_Identity=29.8050139275766, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI209862871, Length=359, Percent_Identity=29.8050139275766, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI216548445, Length=332, Percent_Identity=29.8192771084337, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI163792194, Length=310, Percent_Identity=32.258064516129, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17564890, Length=444, Percent_Identity=29.2792792792793, Blast_Score=171, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6320325, Length=468, Percent_Identity=28.6324786324786, Blast_Score=153, Evalue=4e-38,
Organism=Drosophila melanogaster, GI19921190, Length=443, Percent_Identity=29.5711060948081, Blast_Score=159, Evalue=3e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRM1_THEON (B6YUU9)

Other databases:

- EMBL:   CP000855
- RefSeq:   YP_002307032.1
- GeneID:   7016947
- GenomeReviews:   CP000855_GR
- KEGG:   ton:TON_0648
- HOGENOM:   HBG577872
- OMA:   DAPVFYN
- ProtClustDB:   PRK04338
- HAMAP:   MF_00290
- InterPro:   IPR022923
- InterPro:   IPR002905
- PANTHER:   PTHR10631
- TIGRFAMs:   TIGR00308

Pfam domain/function: PF02005 TRM

EC number: =2.1.1.32

Molecular weight: Translated: 42633; Mature: 42633

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELKEVREGLARILVPKAERIYDAPVFYNPLMALNRDISVLALKALGSRRVLDALSATGI
CCHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHCCH
RGIRYALETPAGEIWLNDINPNAFRLIVDNVKLNFSGELEIEGKMAVLEGEKTLVVTNAD
HHHHHHHCCCCCEEEEECCCCCEEEEEEEEEEEEECCCEEECCEEEEEECCEEEEEECCC
ANRLMNEQFRYFDFVDLDPFGSPMEFLDSALRAVKRRGVLAITATDTGVLCGAYRNACLR
HHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHH
KYLAEPIRGELCHEAGLRILIGTLVRYAAKYDLGVEVLLAYYRDHYFRAFLQLRNGAKTA
HHHHCHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHH
DKSMKQLGYLYQDESGKFEFENTFLPSKATAHGPLWLGPLKNQEFVEELHRLSAEHPLGS
HHHHHHHCCCEECCCCCEEECCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCCCCC
KKTQKFLGLLKDELEVPFFYDTHAVARRHGLEVRKLAGVIEILYEKGYKATRTHFSPTAL
HHHHHHHHHHHHHCCCCEEECHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHCCCCCCCC
KTDAPFEEVLEALRELQ
CCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MELKEVREGLARILVPKAERIYDAPVFYNPLMALNRDISVLALKALGSRRVLDALSATGI
CCHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHCCH
RGIRYALETPAGEIWLNDINPNAFRLIVDNVKLNFSGELEIEGKMAVLEGEKTLVVTNAD
HHHHHHHCCCCCEEEEECCCCCEEEEEEEEEEEEECCCEEECCEEEEEECCEEEEEECCC
ANRLMNEQFRYFDFVDLDPFGSPMEFLDSALRAVKRRGVLAITATDTGVLCGAYRNACLR
HHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHH
KYLAEPIRGELCHEAGLRILIGTLVRYAAKYDLGVEVLLAYYRDHYFRAFLQLRNGAKTA
HHHHCHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHH
DKSMKQLGYLYQDESGKFEFENTFLPSKATAHGPLWLGPLKNQEFVEELHRLSAEHPLGS
HHHHHHHCCCEECCCCCEEECCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCCCCC
KKTQKFLGLLKDELEVPFFYDTHAVARRHGLEVRKLAGVIEILYEKGYKATRTHFSPTAL
HHHHHHHHHHHHHCCCCEEECHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHCCCCCCCC
KTDAPFEEVLEALRELQ
CCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA