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The map label for this gene is glyS [H]

Identifier: 212223791

GI number: 212223791

Start: 599611

End: 601314

Strand: Reverse

Name: glyS [H]

Synonym: TON_0643

Alternate gene names: 212223791

Gene position: 601314-599611 (Counterclockwise)

Preceding gene: 212223795

Following gene: 212223790

Centisome position: 32.55

GC content: 53.93

Gene sequence:

>1704_bases
ATGGCGGATAAGTACGAGATTCTTCAGGACCTCATGAGGAGGAGAGGCTTTGCCTGGGGTAGCTTTGAAATCTACGGTGG
TACGAGAGGTTTTTACGATTACGGTCCTCTGGGGGCTACAATAAAGCGCAAAATAGAGCAGAAGATAAGGGAAGCCTTCC
AGAGGGAGGGTTTCTTCGAGCTTGAAACCCCGGACATAACCCCCGAAAAGGTCTTCATAGCGAGCGGCCACGTTGACAAG
TTCGTTGACCCGCTTGTTGAGTGTAGGAAGTGCGGCGCCAGGTTCAGGGCCGACCACCTCGTTGAGGAGGCCCTCGGCAT
AGACACCGAGGGTATGAGTGCCGACCATTTGACCCGGCTCATAAGGGAGCACGACATAAAATGCCCCGAGTGCGGCGGTG
AGCTTTCCGATGTCTGGTACTTCAACCTCATGTTCGAGACTAAAATCGGTCCCTACGGCGACCAGAAGGGTTATCTGAGG
CCTGAAACGGCCCAGGGCATTTTCGTGAACTTCAGGCGCCTAAACGCTTTCGCAAGGAACAAACTTCCCTTCGGCGTTTT
CCAGATAGGCAAGGCCTACCGTAATGAGATCTCACCGAGACAGGGAATGCTCCGCCTGAGGGAGTTCACGCAGGCCGAGG
CGGAGATATTCTTCAACCCGAAGGAGACTGAACATCCGCACTTCGACGAGGTCAAGGACGAGGTTCTGAGGCTCTATCCG
ATAGAGAACCAGCTCAAGAACCTCGGGGAAATCGAGATGACGGCTGAAGAGGCAGTCAAGAAGGGCTACATCATGAACAC
ATTCTTCGCATACTACATGGTTATGGTCAAGCGCGTTCTCCTCGACATAGGCATTCCCGAGAGTGCCATACGCTTCCGCC
AGCAGCTACCCGAGGAGAGGGCCCACTACTCCAGCGACACATGGGATGTTGAGATACACAGCGAGCGCTTTGGGTGGGTG
GAGTGCGTCGGCATAGCATACCGTGGAGATTATGACCTCAGCAAGCACATGAAGATGAGCGGGGCAGATTTAACCGTCCT
CATCCATTATGACGAACCAAAGATAGTCAAACGCCTGGTTGTCAGCCTCAACATGAAGCGTGTCGGACCGAAGCTCAAAG
GCGACGCCAAGAGGATAAACGAGCTGATCCAGAGCTGGGACGAGGAGAAGCTCAGGAACCTGGTGGATGTGCTGGAGAGG
GACGGCAGGATAACCATCGAGGGCTACGAGCTTGAGAAGGATGACTTCATAATCAAAGAAGTCGAGGAGAAGATAACTGG
CGAGAAGATAGTGCCCCATGTCCTCGAGCCAAGCTTTGGTATCGACAGGCCGTTCTACCTGCTCCTCGAGAACTCTCTCG
TCATAGAGGATGATAGGACATACCTCAGGCTCAAGAAGGACATGGCACCGATTGAAGTTGCCGTCCTTCCGCTCGTTGCG
AAGGAACCGCTCAAGAGTATAGCCTACGAGGTGTTTAGAAAGCTCCAGAAAGCGGGCTTCATAGCGGTGTATGACGAGAA
GGACACGATAGGGAGGCGCTACATCAGGTACGACGAGATTGGAACGCCCTACTGCGTCACGATAGACAACCAGACACCCG
AGGATGGCACGGTAACGATCAGGGACCGCGACACGAGGGAGCAGGTCAGGGTGAAGATAGAGGAGCTCCCGGAGAAGCTG
AGAGAACTGATTTTTGGGGAGTGA

Upstream 100 bases:

>100_bases
CGGTTTATAACTCTTTCCCTCTGCCTATCTTGACGATTAGACGAACGAACGCCCAAAAGGTTTTTATGAAAATCCCTTAC
TTGAGTTTCAGGTGGTGAGT

Downstream 100 bases:

>100_bases
GTTCTTCTTTTTTCTAATATCCTTTCTGGTGTCTCTCATGAAGATCCACCTCATCTACCGTCGCCTTCCGAACAGGGTTC
TTGAGAGGTATGATGATGTC

Product: glycyl-tRNA synthetase

Products: NA

Alternate protein names: Glycine--tRNA ligase; GlyRS [H]

Number of amino acids: Translated: 567; Mature: 566

Protein sequence:

>567_residues
MADKYEILQDLMRRRGFAWGSFEIYGGTRGFYDYGPLGATIKRKIEQKIREAFQREGFFELETPDITPEKVFIASGHVDK
FVDPLVECRKCGARFRADHLVEEALGIDTEGMSADHLTRLIREHDIKCPECGGELSDVWYFNLMFETKIGPYGDQKGYLR
PETAQGIFVNFRRLNAFARNKLPFGVFQIGKAYRNEISPRQGMLRLREFTQAEAEIFFNPKETEHPHFDEVKDEVLRLYP
IENQLKNLGEIEMTAEEAVKKGYIMNTFFAYYMVMVKRVLLDIGIPESAIRFRQQLPEERAHYSSDTWDVEIHSERFGWV
ECVGIAYRGDYDLSKHMKMSGADLTVLIHYDEPKIVKRLVVSLNMKRVGPKLKGDAKRINELIQSWDEEKLRNLVDVLER
DGRITIEGYELEKDDFIIKEVEEKITGEKIVPHVLEPSFGIDRPFYLLLENSLVIEDDRTYLRLKKDMAPIEVAVLPLVA
KEPLKSIAYEVFRKLQKAGFIAVYDEKDTIGRRYIRYDEIGTPYCVTIDNQTPEDGTVTIRDRDTREQVRVKIEELPEKL
RELIFGE

Sequences:

>Translated_567_residues
MADKYEILQDLMRRRGFAWGSFEIYGGTRGFYDYGPLGATIKRKIEQKIREAFQREGFFELETPDITPEKVFIASGHVDK
FVDPLVECRKCGARFRADHLVEEALGIDTEGMSADHLTRLIREHDIKCPECGGELSDVWYFNLMFETKIGPYGDQKGYLR
PETAQGIFVNFRRLNAFARNKLPFGVFQIGKAYRNEISPRQGMLRLREFTQAEAEIFFNPKETEHPHFDEVKDEVLRLYP
IENQLKNLGEIEMTAEEAVKKGYIMNTFFAYYMVMVKRVLLDIGIPESAIRFRQQLPEERAHYSSDTWDVEIHSERFGWV
ECVGIAYRGDYDLSKHMKMSGADLTVLIHYDEPKIVKRLVVSLNMKRVGPKLKGDAKRINELIQSWDEEKLRNLVDVLER
DGRITIEGYELEKDDFIIKEVEEKITGEKIVPHVLEPSFGIDRPFYLLLENSLVIEDDRTYLRLKKDMAPIEVAVLPLVA
KEPLKSIAYEVFRKLQKAGFIAVYDEKDTIGRRYIRYDEIGTPYCVTIDNQTPEDGTVTIRDRDTREQVRVKIEELPEKL
RELIFGE
>Mature_566_residues
ADKYEILQDLMRRRGFAWGSFEIYGGTRGFYDYGPLGATIKRKIEQKIREAFQREGFFELETPDITPEKVFIASGHVDKF
VDPLVECRKCGARFRADHLVEEALGIDTEGMSADHLTRLIREHDIKCPECGGELSDVWYFNLMFETKIGPYGDQKGYLRP
ETAQGIFVNFRRLNAFARNKLPFGVFQIGKAYRNEISPRQGMLRLREFTQAEAEIFFNPKETEHPHFDEVKDEVLRLYPI
ENQLKNLGEIEMTAEEAVKKGYIMNTFFAYYMVMVKRVLLDIGIPESAIRFRQQLPEERAHYSSDTWDVEIHSERFGWVE
CVGIAYRGDYDLSKHMKMSGADLTVLIHYDEPKIVKRLVVSLNMKRVGPKLKGDAKRINELIQSWDEEKLRNLVDVLERD
GRITIEGYELEKDDFIIKEVEEKITGEKIVPHVLEPSFGIDRPFYLLLENSLVIEDDRTYLRLKKDMAPIEVAVLPLVAK
EPLKSIAYEVFRKLQKAGFIAVYDEKDTIGRRYIRYDEIGTPYCVTIDNQTPEDGTVTIRDRDTREQVRVKIEELPEKLR
ELIFGE

Specific function: Catalyzes the attachment of glycine to tRNA(Gly) [H]

COG id: COG0423

COG function: function code J; Glycyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI116805340, Length=592, Percent_Identity=38.3445945945946, Blast_Score=392, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17554996, Length=592, Percent_Identity=39.8648648648649, Blast_Score=398, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI32564541, Length=592, Percent_Identity=39.8648648648649, Blast_Score=398, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6319597, Length=627, Percent_Identity=36.2041467304625, Blast_Score=369, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6325338, Length=590, Percent_Identity=36.7796610169491, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24664462, Length=574, Percent_Identity=39.7212543554007, Blast_Score=403, Evalue=1e-112,
Organism=Drosophila melanogaster, GI21357965, Length=574, Percent_Identity=39.7212543554007, Blast_Score=402, Evalue=1e-112,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR022960
- InterPro:   IPR002315 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 66023; Mature: 65891

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADKYEILQDLMRRRGFAWGSFEIYGGTRGFYDYGPLGATIKRKIEQKIREAFQREGFFE
CCCHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEE
LETPDITPEKVFIASGHVDKFVDPLVECRKCGARFRADHLVEEALGIDTEGMSADHLTRL
ECCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHH
IREHDIKCPECGGELSDVWYFNLMFETKIGPYGDQKGYLRPETAQGIFVNFRRLNAFARN
HHHHCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHC
KLPFGVFQIGKAYRNEISPRQGMLRLREFTQAEAEIFFNPKETEHPHFDEVKDEVLRLYP
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHCC
IENQLKNLGEIEMTAEEAVKKGYIMNTFFAYYMVMVKRVLLDIGIPESAIRFRQQLPEER
CHHHHHCCCCEEECHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHH
AHYSSDTWDVEIHSERFGWVECVGIAYRGDYDLSKHMKMSGADLTVLIHYDEPKIVKRLV
HCCCCCCEEEEECCCCCCEEEEEEEEEECCCCHHHHHCCCCCCEEEEEECCCHHHHHHHH
VSLNMKRVGPKLKGDAKRINELIQSWDEEKLRNLVDVLERDGRITIEGYELEKDDFIIKE
HHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHH
VEEKITGEKIVPHVLEPSFGIDRPFYLLLENSLVIEDDRTYLRLKKDMAPIEVAVLPLVA
HHHHHCCCCCCHHHCCCCCCCCCCEEEEEECCEEEECCCHHHHHHHCCCCEEEEEEHHHH
KEPLKSIAYEVFRKLQKAGFIAVYDEKDTIGRRYIRYDEIGTPYCVTIDNQTPEDGTVTI
HHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHEEEECCCCCCEEEEECCCCCCCCEEEE
RDRDTREQVRVKIEELPEKLRELIFGE
ECCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ADKYEILQDLMRRRGFAWGSFEIYGGTRGFYDYGPLGATIKRKIEQKIREAFQREGFFE
CCHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEE
LETPDITPEKVFIASGHVDKFVDPLVECRKCGARFRADHLVEEALGIDTEGMSADHLTRL
ECCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHH
IREHDIKCPECGGELSDVWYFNLMFETKIGPYGDQKGYLRPETAQGIFVNFRRLNAFARN
HHHHCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHC
KLPFGVFQIGKAYRNEISPRQGMLRLREFTQAEAEIFFNPKETEHPHFDEVKDEVLRLYP
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHCC
IENQLKNLGEIEMTAEEAVKKGYIMNTFFAYYMVMVKRVLLDIGIPESAIRFRQQLPEER
CHHHHHCCCCEEECHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHH
AHYSSDTWDVEIHSERFGWVECVGIAYRGDYDLSKHMKMSGADLTVLIHYDEPKIVKRLV
HCCCCCCEEEEECCCCCCEEEEEEEEEECCCCHHHHHCCCCCCEEEEEECCCHHHHHHHH
VSLNMKRVGPKLKGDAKRINELIQSWDEEKLRNLVDVLERDGRITIEGYELEKDDFIIKE
HHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHH
VEEKITGEKIVPHVLEPSFGIDRPFYLLLENSLVIEDDRTYLRLKKDMAPIEVAVLPLVA
HHHHHCCCCCCHHHCCCCCCCCCCEEEEEECCEEEECCCHHHHHHHCCCCEEEEEEHHHH
KEPLKSIAYEVFRKLQKAGFIAVYDEKDTIGRRYIRYDEIGTPYCVTIDNQTPEDGTVTI
HHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHEEEECCCCCCEEEEECCCCCCCCEEEE
RDRDTREQVRVKIEELPEKLRELIFGE
ECCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA