Click here to switch to the map view.

The map label for this gene is nfi [H]

Identifier: 212223706

GI number: 212223706

Start: 512808

End: 513377

Strand: Reverse

Name: nfi [H]

Synonym: TON_0558

Alternate gene names: 212223706

Gene position: 513377-512808 (Counterclockwise)

Preceding gene: 212223707

Following gene: 212223705

Centisome position: 27.79

GC content: 51.4

Gene sequence:

>570_bases
ATGGAATTGGGGAAAAAGCTCAAAAAAATTGAAGAAACCCAGCGAAAGCTTGCCCTGAGAGTGGTGGAGAGCCCGCTCGA
GATCGAAAAAGTGCAAACTGTCGCGGCTGTGGACGTTTCATACAGAGAAAACACAGCCAGAGCTGCCTTTCTCCTCTGCT
CCTTCCCAGACTGCGAGGTTCTAAAGACAAAGGTCGTGGAAACTGAGGTAAGCTTTCCCTACGTACCCACCTTCTTTTTC
CTGCGAGAGACGAGGCCAATTCTGCTGGTTCTCCGTGGTGAGGATTTTGACCTCCTTCTTATTGATGGTCACGGAAAGGC
CCATCCACGGGGCTACGGACTGGCTTCCCACGTCGGCTTGCTAATCGGAAGACCAACCATAGGAGTTGCCAAGAAGTTCC
TCCGCGGTGCTCCTGAGAGGTCCTTCGTGAGGGTGGGAAAAGCTTATGTAAGCGTCGGATATTTAATCGATTTAGACTCT
GCGGCCAGAATAGTTGAGACCCTGCTGGAGCGAGACTATCCAAAACCACTGAGGCTCGCAGACAGACTGTCAAAGAGGGG
AAGCTTATGA

Upstream 100 bases:

>100_bases
CGAGGACCTGACGAGGGCGAAGCTCAACAGGAACCTTGAGGAGAAGCTCGGAAAGGTGTTAAATGATTAACGAATTACAT
AATTAGGTGATTCGGTAATT

Downstream 100 bases:

>100_bases
AGAGGCTCAAACTGCTCATCCTGCTAGCAAAGAAAGGAGCGATAGGAGAAAAGGTCAAAATAACTCTAAGAGAGCTTGCA
AACGAGCTTGGTGTATCACC

Product: endonuclease V

Products: NA

Alternate protein names: Deoxyinosine 3'endonuclease; Deoxyribonuclease V; DNase V [H]

Number of amino acids: Translated: 189; Mature: 189

Protein sequence:

>189_residues
MELGKKLKKIEETQRKLALRVVESPLEIEKVQTVAAVDVSYRENTARAAFLLCSFPDCEVLKTKVVETEVSFPYVPTFFF
LRETRPILLVLRGEDFDLLLIDGHGKAHPRGYGLASHVGLLIGRPTIGVAKKFLRGAPERSFVRVGKAYVSVGYLIDLDS
AARIVETLLERDYPKPLRLADRLSKRGSL

Sequences:

>Translated_189_residues
MELGKKLKKIEETQRKLALRVVESPLEIEKVQTVAAVDVSYRENTARAAFLLCSFPDCEVLKTKVVETEVSFPYVPTFFF
LRETRPILLVLRGEDFDLLLIDGHGKAHPRGYGLASHVGLLIGRPTIGVAKKFLRGAPERSFVRVGKAYVSVGYLIDLDS
AARIVETLLERDYPKPLRLADRLSKRGSL
>Mature_189_residues
MELGKKLKKIEETQRKLALRVVESPLEIEKVQTVAAVDVSYRENTARAAFLLCSFPDCEVLKTKVVETEVSFPYVPTFFF
LRETRPILLVLRGEDFDLLLIDGHGKAHPRGYGLASHVGLLIGRPTIGVAKKFLRGAPERSFVRVGKAYVSVGYLIDLDS
AARIVETLLERDYPKPLRLADRLSKRGSL

Specific function: Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. Acts in DNA repair [H]

COG id: COG1515

COG function: function code L; Deoxyinosine 3'endonuclease (endonuclease V)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the endonuclease V family [H]

Homologues:

Organism=Homo sapiens, GI31542775, Length=201, Percent_Identity=30.3482587064677, Blast_Score=73, Evalue=2e-13,
Organism=Escherichia coli, GI87082357, Length=209, Percent_Identity=32.5358851674641, Blast_Score=94, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007581 [H]

Pfam domain/function: PF04493 Endonuclease_5 [H]

EC number: =3.1.21.7 [H]

Molecular weight: Translated: 21196; Mature: 21196

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELGKKLKKIEETQRKLALRVVESPLEIEKVQTVAAVDVSYRENTARAAFLLCSFPDCEV
CCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEECHHCCCCCEEEEEEECCCCHHH
LKTKVVETEVSFPYVPTFFFLRETRPILLVLRGEDFDLLLIDGHGKAHPRGYGLASHVGL
HHHHHHHHCCCCCCCHHHHEEECCCCEEEEEECCCEEEEEECCCCCCCCCCCCHHHHHHH
LIGRPTIGVAKKFLRGAPERSFVRVGKAYVSVGYLIDLDSAARIVETLLERDYPKPLRLA
HCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHCCCCCHHHHH
DRLSKRGSL
HHHHHCCCC
>Mature Secondary Structure
MELGKKLKKIEETQRKLALRVVESPLEIEKVQTVAAVDVSYRENTARAAFLLCSFPDCEV
CCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEECHHCCCCCEEEEEEECCCCHHH
LKTKVVETEVSFPYVPTFFFLRETRPILLVLRGEDFDLLLIDGHGKAHPRGYGLASHVGL
HHHHHHHHCCCCCCCHHHHEEECCCCEEEEEECCCEEEEEECCCCCCCCCCCCHHHHHHH
LIGRPTIGVAKKFLRGAPERSFVRVGKAYVSVGYLIDLDSAARIVETLLERDYPKPLRLA
HCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHCCCCCHHHHH
DRLSKRGSL
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA