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The map label for this gene is gatD [H]

Identifier: 212223549

GI number: 212223549

Start: 375701

End: 377020

Strand: Reverse

Name: gatD [H]

Synonym: TON_0403

Alternate gene names: 212223549

Gene position: 377020-375701 (Counterclockwise)

Preceding gene: 212223550

Following gene: 212223548

Centisome position: 20.41

GC content: 52.95

Gene sequence:

>1320_bases
ATGCGCAAAGTTGAGCGGTTTATGCAGGAAAAGGGCCTCAGTGTTGGGGATTACGTCAGAATAATCGAAAAGGATAACGG
AAACAAGAGCATCTATGAAGGAATAGTCATGAACCCCTACGAGCTCTCCACCGGCGAGACGCTTACGCTCAAGCTTGACA
ATGGCTACAACATTGGAATCCTCGTTGATCAGATAGTTTCAGTTGAAATCATCGAGAAGGCGAAGCCAAGAGAGGAAGTA
GAGTTCGAAGAAGTGTTTCCGAAAAGGCCAGAGCTTCCAAACGTTGCTATCCTTGGAACGGGCGGAACCATAGCAAGCAG
GATAGACTACAAGACTGGAGCCGTCCATCCGGCTTTCACTGCGGAGGAACTTGCCAAGGCAGTTCCGGAGATTTTTGAAA
TAGCCAACATAACACCGAAGCTCCTTATGAACATCCTCAGTGAGGACATGCGCCCGACGTACTGGGTCAGGATAGCCCAC
GAAGTTGCGGGCATACTGAACAACGGCGAGGACGGCGTCATCATAGCCCACGGGACGGATACGATGGCATACACCGCATC
GGCTTTGAGCTTCATGCTCAGGGACTTGGGCAAACCGGTCATACTCGTCGGCGCTCAAAGAAGCTCGGACAGGCCGAGCA
GCGACGCGGCGATGAACCTCATATGCTCTGTCAGGATGGCAAGGGCTGATTTCGGCGAGGTTGCCGTTGTAATGCATGGG
GAGACGAGTGACACATACTGTCTCGCTCACAGGGGAACCAAGGTCAGGAAGATGCACACCAGCAGGAGGGACGCCTTCAG
GAGCATAAACGATATACCGATAGCCAGAATATGGCCGGGTGGTGAGATTGAGTTCCTCAGAAAGGACTACCGCAGAAGGA
CTGAAAGCGAGGTCTGGGTCGACGACGAGATGGAGGAGAAGGTCGCTTTGGTAAAGGCCTTCCCCGGAATGAGCACCGAG
ATAATAGACTTCTTCGTGGACAAGGGGTACAAGGGCATAGTCATAGAGGGAACCGGTCTCGGCCACGTGCCGACCTACGT
CATAGACTCAATACAACGCGCTACTGAGGAAGGCGTTGCCGTCTGCATGACGAGTCAGTGTCTCTACGGCAGGGTGAATC
TCAACGTTTACTCCACTGGAAGAAGGCTCCTCAAGGCGGGCGTTATTCCGTGCGAAGACATGCTTCCAGAGACGGCTTAC
GTCAAGCTTATGTGGGTGCTTGGCCACACTGATGATCCAAAAGAGGTCAGGGAAATGATGCTCACGAACTATGCGGGCGA
GATAACGCCCTACACGAGGTTTGACACGTTCCTGAGGTGA

Upstream 100 bases:

>100_bases
TTATGACAGAATTCATCTAAATTTTTGGGAGATTCTTGTCATTCATCCAGCCCGAAGGTTTATATTAACCTCTCACCAGT
ATAAGGCGGAGGGAAAGGCT

Downstream 100 bases:

>100_bases
TGACAATGGCGTACGATTACGAGAAGCTTGGTCTTAAGGTCGGTCTTGAAATCCACAGGCAACTCGACACCAAAAAGCTA
TTCTCACCCGTTCCAAGCGA

Product: glutamyl-tRNA(Gln) amidotransferase subunit D

Products: L-aspartate; NH3

Alternate protein names: Glu-ADT subunit D [H]

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MRKVERFMQEKGLSVGDYVRIIEKDNGNKSIYEGIVMNPYELSTGETLTLKLDNGYNIGILVDQIVSVEIIEKAKPREEV
EFEEVFPKRPELPNVAILGTGGTIASRIDYKTGAVHPAFTAEELAKAVPEIFEIANITPKLLMNILSEDMRPTYWVRIAH
EVAGILNNGEDGVIIAHGTDTMAYTASALSFMLRDLGKPVILVGAQRSSDRPSSDAAMNLICSVRMARADFGEVAVVMHG
ETSDTYCLAHRGTKVRKMHTSRRDAFRSINDIPIARIWPGGEIEFLRKDYRRRTESEVWVDDEMEEKVALVKAFPGMSTE
IIDFFVDKGYKGIVIEGTGLGHVPTYVIDSIQRATEEGVAVCMTSQCLYGRVNLNVYSTGRRLLKAGVIPCEDMLPETAY
VKLMWVLGHTDDPKEVREMMLTNYAGEITPYTRFDTFLR

Sequences:

>Translated_439_residues
MRKVERFMQEKGLSVGDYVRIIEKDNGNKSIYEGIVMNPYELSTGETLTLKLDNGYNIGILVDQIVSVEIIEKAKPREEV
EFEEVFPKRPELPNVAILGTGGTIASRIDYKTGAVHPAFTAEELAKAVPEIFEIANITPKLLMNILSEDMRPTYWVRIAH
EVAGILNNGEDGVIIAHGTDTMAYTASALSFMLRDLGKPVILVGAQRSSDRPSSDAAMNLICSVRMARADFGEVAVVMHG
ETSDTYCLAHRGTKVRKMHTSRRDAFRSINDIPIARIWPGGEIEFLRKDYRRRTESEVWVDDEMEEKVALVKAFPGMSTE
IIDFFVDKGYKGIVIEGTGLGHVPTYVIDSIQRATEEGVAVCMTSQCLYGRVNLNVYSTGRRLLKAGVIPCEDMLPETAY
VKLMWVLGHTDDPKEVREMMLTNYAGEITPYTRFDTFLR
>Mature_439_residues
MRKVERFMQEKGLSVGDYVRIIEKDNGNKSIYEGIVMNPYELSTGETLTLKLDNGYNIGILVDQIVSVEIIEKAKPREEV
EFEEVFPKRPELPNVAILGTGGTIASRIDYKTGAVHPAFTAEELAKAVPEIFEIANITPKLLMNILSEDMRPTYWVRIAH
EVAGILNNGEDGVIIAHGTDTMAYTASALSFMLRDLGKPVILVGAQRSSDRPSSDAAMNLICSVRMARADFGEVAVVMHG
ETSDTYCLAHRGTKVRKMHTSRRDAFRSINDIPIARIWPGGEIEFLRKDYRRRTESEVWVDDEMEEKVALVKAFPGMSTE
IIDFFVDKGYKGIVIEGTGLGHVPTYVIDSIQRATEEGVAVCMTSQCLYGRVNLNVYSTGRRLLKAGVIPCEDMLPETAY
VKLMWVLGHTDDPKEVREMMLTNYAGEITPYTRFDTFLR

Specific function: Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-ph

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family. GatD subfamily [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=358, Percent_Identity=32.4022346368715, Blast_Score=124, Evalue=2e-28,
Organism=Escherichia coli, GI1788065, Length=320, Percent_Identity=33.75, Blast_Score=162, Evalue=6e-41,
Organism=Escherichia coli, GI1789327, Length=341, Percent_Identity=31.6715542521994, Blast_Score=127, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI193207372, Length=288, Percent_Identity=35.0694444444444, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17558280, Length=288, Percent_Identity=35.0694444444444, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI193207374, Length=288, Percent_Identity=35.0694444444444, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17558278, Length=288, Percent_Identity=35.0694444444444, Blast_Score=149, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6320527, Length=345, Percent_Identity=25.2173913043478, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6323189, Length=268, Percent_Identity=27.9850746268657, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6323187, Length=268, Percent_Identity=27.9850746268657, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6323186, Length=268, Percent_Identity=27.9850746268657, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6323184, Length=268, Percent_Identity=27.9850746268657, Blast_Score=84, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24639609, Length=303, Percent_Identity=32.6732673267327, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24645530, Length=280, Percent_Identity=33.5714285714286, Blast_Score=138, Evalue=8e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827
- InterPro:   IPR011878 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: 3.5.1.1

Molecular weight: Translated: 49164; Mature: 49164

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKVERFMQEKGLSVGDYVRIIEKDNGNKSIYEGIVMNPYELSTGETLTLKLDNGYNIGI
CCHHHHHHHHCCCCCCCEEEEEECCCCCHHHHCCEEECCEEECCCCEEEEEECCCCEEEE
LVDQIVSVEIIEKAKPREEVEFEEVFPKRPELPNVAILGTGGTIASRIDYKTGAVHPAFT
HHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCEEEEECCCCHHHCCCCCCCCCCCCCC
AEELAKAVPEIFEIANITPKLLMNILSEDMRPTYWVRIAHEVAGILNNGEDGVIIAHGTD
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHEEEEHHHHHHHHCCCCCCEEEEECCC
TMAYTASALSFMLRDLGKPVILVGAQRSSDRPSSDAAMNLICSVRMARADFGEVAVVMHG
CHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEC
ETSDTYCLAHRGTKVRKMHTSRRDAFRSINDIPIARIWPGGEIEFLRKDYRRRTESEVWV
CCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCC
DDEMEEKVALVKAFPGMSTEIIDFFVDKGYKGIVIEGTGLGHVPTYVIDSIQRATEEGVA
CCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCEE
VCMTSQCLYGRVNLNVYSTGRRLLKAGVIPCEDMLPETAYVKLMWVLGHTDDPKEVREMM
EEEECCEEEEEEEEEEEHHHHHHHHHCCCCHHHHCCCCHHEEEEEEECCCCCHHHHHHHH
LTNYAGEITPYTRFDTFLR
HHHCCCCCCCCHHHHHHCC
>Mature Secondary Structure
MRKVERFMQEKGLSVGDYVRIIEKDNGNKSIYEGIVMNPYELSTGETLTLKLDNGYNIGI
CCHHHHHHHHCCCCCCCEEEEEECCCCCHHHHCCEEECCEEECCCCEEEEEECCCCEEEE
LVDQIVSVEIIEKAKPREEVEFEEVFPKRPELPNVAILGTGGTIASRIDYKTGAVHPAFT
HHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCEEEEECCCCHHHCCCCCCCCCCCCCC
AEELAKAVPEIFEIANITPKLLMNILSEDMRPTYWVRIAHEVAGILNNGEDGVIIAHGTD
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHEEEEHHHHHHHHCCCCCCEEEEECCC
TMAYTASALSFMLRDLGKPVILVGAQRSSDRPSSDAAMNLICSVRMARADFGEVAVVMHG
CHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEC
ETSDTYCLAHRGTKVRKMHTSRRDAFRSINDIPIARIWPGGEIEFLRKDYRRRTESEVWV
CCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCC
DDEMEEKVALVKAFPGMSTEIIDFFVDKGYKGIVIEGTGLGHVPTYVIDSIQRATEEGVA
CCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCEE
VCMTSQCLYGRVNLNVYSTGRRLLKAGVIPCEDMLPETAYVKLMWVLGHTDDPKEVREMM
EEEECCEEEEEEEEEEEHHHHHHHHHCCCCHHHHCCCCHHEEEEEEECCCCCHHHHHHHH
LTNYAGEITPYTRFDTFLR
HHHCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-asparagine; H2O

Specific reaction: L-asparagine + H2O = L-aspartate + NH3

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: 5-Bromo-4-oxo-L-norvaline; Hg2+; NH3 [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]