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The map label for this gene is gatE [H]

Identifier: 212223548

GI number: 212223548

Start: 373803

End: 375695

Strand: Reverse

Name: gatE [H]

Synonym: TON_0402

Alternate gene names: 212223548

Gene position: 375695-373803 (Counterclockwise)

Preceding gene: 212223549

Following gene: 212223543

Centisome position: 20.33

GC content: 52.93

Gene sequence:

>1893_bases
ATGGCGTACGATTACGAGAAGCTTGGTCTTAAGGTCGGTCTTGAAATCCACAGGCAACTCGACACCAAAAAGCTATTCTC
ACCCGTTCCAAGCGAGCTGAGCGACGAGGTGGAGTTTACCTTCCAGCGTCGTTTAAGGCCAACTATAAGCGAGCTCGGCG
AGATTGATCCGGCTGCTCTGGAGGAGTTCAAGAAGGGGAGGACTTACATCTACGAGGGCAGTTACGAACTGGCAGACCTC
GTTTACATTGACGAAGAGCCGCCACACATGCCGGATGAAGAAGCCCTCAAGGTCGCCCTTCAGATTTCCTACCTTCTCAA
CGCCACTCCCATTGACGAGGTTCACTTCATGCGTAAAATCGTCATAGATGGCTCCAACGTCTCAGGCTTCCAGAGGACGG
CAATAATAGCCATGAACGGAAAGGTGGACACACCGTGGGGAAGTGTTGGAATCCCAACGATTTGCCTTGAAGAGGACGCC
TGTAGAATAGTCGAGAGGAAAGACAAGGAGGTAATCTACCGCCTCGACAGGCTGGGTATTCCTCTCGTTGAGATAAGCAC
GACTCCGGACATACACCACCCGGAGCAGGCAAAGGTGGTCGCCAAATACATAGGCGACGCTCTGAGAGCGACGAGGAAAG
TCAAGCGCGGTCTTGGAACCATCAGGCAGGATCTCAACATTTCAATAAAGGGCGGTGCCAGAGTAGAGATCAAGGGGGTC
CAGGAGCTAGATATGATACCAATCATCATTGAGAGAGAAATCGAAAGGCAGCTCAAGCTCCTCGAGATACGAGACGAGCT
GAGGAGGCGCGGAGTCAAGCCAGAGGACATCAAAGAGGAATTCTACGACGTCACTGAGATATTCGAGAACACTGGCTCTA
AGATAATAGCGCGCACGATAAATAACGGTGGAAAGGTCCTCGCCGTTAAGCTCCCCAAGTTCCGCGGGCTCATCGGGAAA
GAGATTCAGCCCGGCAGGAGGCTCGGAACTGAGATGGCTGACAGAGCCAAGAAATACGTGAAAGGTATCTTCCACATTGA
TGAATTACCGAATTATGGAATTACAGAATTGGAGGTTAATGCAGTTATCGAAAAACTCGGTCTCGGCGAGGATGACGCCT
TCGTCCTCGTCGCCGCAGAGGAGGAAACTGCCAAGAAAGCACTCCGCGAGGTTCTCCAGAGGGCGAGGGAGGCTATAGAC
GGCGTGCCGGAGGAAACAAGAAGAGCTCTACCCGATGGAAACACCCAGTACATGCGCCCGCTTCCCGGCAAGGCGAGGAT
GTATCCCGAGACAGACATACCGCCGATACTCATCACGGAGGATATGAAAGAGGAGATACTCGCCAACCTGCCAGAGCTCC
CGCAGGAGAGGGCCGAACGCTACGTAAAGGAGTACAATATCGACAAGAGCCTTGCTGAAACCCTTGTAAATGACGAACGC
GACGAGCTCTTCGAAGAGCTCATAGCGAAGGGTGTCAAGCCGTCCCTCGCTGCATCAATACTCGTCGTCGTACTCAAGGG
TCTCAAAAAGGAGGTTCCCATCGAAAACATCACCGACGAGCACATCAGGGATGCTTTTGAGCTCTATCTCAACGGCAAGA
TAGCCAAGGAGGCCTTCGAAGAGATATTCAAGGAACTCGCGCTTCACCCAGAGAAGACGGCCCAGCAGGTTGCTGAAGAA
AAGGGCCTGACGCTGCTCAGCGAGGAGGAAGTTGAGCGCATCATAGACGAAGTCATCCAGCAGAACATCGAAGTCATAAA
GGCCAAGGGCATGGGAGCGATGGGTATGATAATGGGCCGCGCTATGGCGAAGCTCCGCGGAAGGGCCGACGGAAAGCTCG
TGAGCCAGCTCGTAAAGAAAAAGCTGAAGGAAGTCCAAGGATCCGCACCTTGA

Upstream 100 bases:

>100_bases
ACACTGATGATCCAAAAGAGGTCAGGGAAATGATGCTCACGAACTATGCGGGCGAGATAACGCCCTACACGAGGTTTGAC
ACGTTCCTGAGGTGATGACA

Downstream 100 bases:

>100_bases
AGTTTCCTTTTAAGTTCCATTTTATTCTTTGAATTCATCAAATGCCAAGGAATTTGCGTATTTTGAATTTGAGATGACCA
AAAAACACACTGTTCAGATA

Product: glutamyl-tRNA(Gln) amidotransferase subunit E

Products: NA

Alternate protein names: Glu-ADT subunit E [H]

Number of amino acids: Translated: 630; Mature: 629

Protein sequence:

>630_residues
MAYDYEKLGLKVGLEIHRQLDTKKLFSPVPSELSDEVEFTFQRRLRPTISELGEIDPAALEEFKKGRTYIYEGSYELADL
VYIDEEPPHMPDEEALKVALQISYLLNATPIDEVHFMRKIVIDGSNVSGFQRTAIIAMNGKVDTPWGSVGIPTICLEEDA
CRIVERKDKEVIYRLDRLGIPLVEISTTPDIHHPEQAKVVAKYIGDALRATRKVKRGLGTIRQDLNISIKGGARVEIKGV
QELDMIPIIIEREIERQLKLLEIRDELRRRGVKPEDIKEEFYDVTEIFENTGSKIIARTINNGGKVLAVKLPKFRGLIGK
EIQPGRRLGTEMADRAKKYVKGIFHIDELPNYGITELEVNAVIEKLGLGEDDAFVLVAAEEETAKKALREVLQRAREAID
GVPEETRRALPDGNTQYMRPLPGKARMYPETDIPPILITEDMKEEILANLPELPQERAERYVKEYNIDKSLAETLVNDER
DELFEELIAKGVKPSLAASILVVVLKGLKKEVPIENITDEHIRDAFELYLNGKIAKEAFEEIFKELALHPEKTAQQVAEE
KGLTLLSEEEVERIIDEVIQQNIEVIKAKGMGAMGMIMGRAMAKLRGRADGKLVSQLVKKKLKEVQGSAP

Sequences:

>Translated_630_residues
MAYDYEKLGLKVGLEIHRQLDTKKLFSPVPSELSDEVEFTFQRRLRPTISELGEIDPAALEEFKKGRTYIYEGSYELADL
VYIDEEPPHMPDEEALKVALQISYLLNATPIDEVHFMRKIVIDGSNVSGFQRTAIIAMNGKVDTPWGSVGIPTICLEEDA
CRIVERKDKEVIYRLDRLGIPLVEISTTPDIHHPEQAKVVAKYIGDALRATRKVKRGLGTIRQDLNISIKGGARVEIKGV
QELDMIPIIIEREIERQLKLLEIRDELRRRGVKPEDIKEEFYDVTEIFENTGSKIIARTINNGGKVLAVKLPKFRGLIGK
EIQPGRRLGTEMADRAKKYVKGIFHIDELPNYGITELEVNAVIEKLGLGEDDAFVLVAAEEETAKKALREVLQRAREAID
GVPEETRRALPDGNTQYMRPLPGKARMYPETDIPPILITEDMKEEILANLPELPQERAERYVKEYNIDKSLAETLVNDER
DELFEELIAKGVKPSLAASILVVVLKGLKKEVPIENITDEHIRDAFELYLNGKIAKEAFEEIFKELALHPEKTAQQVAEE
KGLTLLSEEEVERIIDEVIQQNIEVIKAKGMGAMGMIMGRAMAKLRGRADGKLVSQLVKKKLKEVQGSAP
>Mature_629_residues
AYDYEKLGLKVGLEIHRQLDTKKLFSPVPSELSDEVEFTFQRRLRPTISELGEIDPAALEEFKKGRTYIYEGSYELADLV
YIDEEPPHMPDEEALKVALQISYLLNATPIDEVHFMRKIVIDGSNVSGFQRTAIIAMNGKVDTPWGSVGIPTICLEEDAC
RIVERKDKEVIYRLDRLGIPLVEISTTPDIHHPEQAKVVAKYIGDALRATRKVKRGLGTIRQDLNISIKGGARVEIKGVQ
ELDMIPIIIEREIERQLKLLEIRDELRRRGVKPEDIKEEFYDVTEIFENTGSKIIARTINNGGKVLAVKLPKFRGLIGKE
IQPGRRLGTEMADRAKKYVKGIFHIDELPNYGITELEVNAVIEKLGLGEDDAFVLVAAEEETAKKALREVLQRAREAIDG
VPEETRRALPDGNTQYMRPLPGKARMYPETDIPPILITEDMKEEILANLPELPQERAERYVKEYNIDKSLAETLVNDERD
ELFEELIAKGVKPSLAASILVVVLKGLKKEVPIENITDEHIRDAFELYLNGKIAKEAFEEIFKELALHPEKTAQQVAEEK
GLTLLSEEEVERIIDEVIQQNIEVIKAKGMGAMGMIMGRAMAKLRGRADGKLVSQLVKKKLKEVQGSAP

Specific function: Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-ph

COG id: COG2511

COG function: function code J; Archaeal Glu-tRNAGln amidotransferase subunit E (contains GAD domain)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatE subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004115
- InterPro:   IPR023168
- InterPro:   IPR004414
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02938 GAD; PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: NA

Molecular weight: Translated: 71109; Mature: 70978

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYDYEKLGLKVGLEIHRQLDTKKLFSPVPSELSDEVEFTFQRRLRPTISELGEIDPAAL
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHCCCCHHHH
EEFKKGRTYIYEGSYELADLVYIDEEPPHMPDEEALKVALQISYLLNATPIDEVHFMRKI
HHHHCCCEEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
VIDGSNVSGFQRTAIIAMNGKVDTPWGSVGIPTICLEEDACRIVERKDKEVIYRLDRLGI
HCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEECHHHHHHHHHCCHHHHHHHHHCCC
PLVEISTTPDIHHPEQAKVVAKYIGDALRATRKVKRGLGTIRQDLNISIKGGARVEIKGV
CEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCEEEECCC
QELDMIPIIIEREIERQLKLLEIRDELRRRGVKPEDIKEEFYDVTEIFENTGSKIIARTI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEHEEC
NNGGKVLAVKLPKFRGLIGKEIQPGRRLGTEMADRAKKYVKGIFHIDELPNYGITELEVN
CCCCEEEEEECCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
AVIEKLGLGEDDAFVLVAAEEETAKKALREVLQRAREAIDGVPEETRRALPDGNTQYMRP
HHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCC
LPGKARMYPETDIPPILITEDMKEEILANLPELPQERAERYVKEYNIDKSLAETLVNDER
CCCCCCCCCCCCCCCEEECCHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCH
DELFEELIAKGVKPSLAASILVVVLKGLKKEVPIENITDEHIRDAFELYLNGKIAKEAFE
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHH
EIFKELALHPEKTAQQVAEEKGLTLLSEEEVERIIDEVIQQNIEVIKAKGMGAMGMIMGR
HHHHHHCCCCHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
AMAKLRGRADGKLVSQLVKKKLKEVQGSAP
HHHHHHCCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AYDYEKLGLKVGLEIHRQLDTKKLFSPVPSELSDEVEFTFQRRLRPTISELGEIDPAAL
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHCCCCHHHH
EEFKKGRTYIYEGSYELADLVYIDEEPPHMPDEEALKVALQISYLLNATPIDEVHFMRKI
HHHHCCCEEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
VIDGSNVSGFQRTAIIAMNGKVDTPWGSVGIPTICLEEDACRIVERKDKEVIYRLDRLGI
HCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEECHHHHHHHHHCCHHHHHHHHHCCC
PLVEISTTPDIHHPEQAKVVAKYIGDALRATRKVKRGLGTIRQDLNISIKGGARVEIKGV
CEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCEEEECCC
QELDMIPIIIEREIERQLKLLEIRDELRRRGVKPEDIKEEFYDVTEIFENTGSKIIARTI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEHEEC
NNGGKVLAVKLPKFRGLIGKEIQPGRRLGTEMADRAKKYVKGIFHIDELPNYGITELEVN
CCCCEEEEEECCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
AVIEKLGLGEDDAFVLVAAEEETAKKALREVLQRAREAIDGVPEETRRALPDGNTQYMRP
HHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCC
LPGKARMYPETDIPPILITEDMKEEILANLPELPQERAERYVKEYNIDKSLAETLVNDER
CCCCCCCCCCCCCCCEEECCHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCH
DELFEELIAKGVKPSLAASILVVVLKGLKKEVPIENITDEHIRDAFELYLNGKIAKEAFE
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHH
EIFKELALHPEKTAQQVAEEKGLTLLSEEEVERIIDEVIQQNIEVIKAKGMGAMGMIMGR
HHHHHHCCCCHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
AMAKLRGRADGKLVSQLVKKKLKEVQGSAP
HHHHHHCCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA